Rroxscaffold_6G00400330

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000579
Physical Location & Seq
Forward (+)
22448487 .. 22453105
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400330.1

Sequence Viewer

Length: 1290 bp
ATGAGAAAACTACTTGCAAGCTTTTTGATAGTTTGCATTTTTACTACATCGTATTGCCAAGAAACTTTTAATGTGCTTGACTACGGTGCTGTTGGAGATGGCCAAACCGATGATTCTGAAGCTTTCTTGAAAGCATGGAGTGCTTTATGTGCAGCAAAAGGAACACCAGAACTTGTAATACCAATGGAAAAAACATTCCTTTTGCAACCTACAAAATTCTCAGGTCCTTGCATATCCAATGGCTCTATCGATGTTGAGATTTTGGGGAAAATTGTGGCACCCAAAACTCCTCAAGAATGGAAAGAGTGTGAAAAAGAATCCTGGCTGCTCTTTTCAGAGGTGAACAACCTGATCATTAATGGAAATGAAACAGGAGTAATCAATGGGAATGGTTTACCTTGGTGGACAGAAGTGAATAAGTCGGAAGTAGAGAAGTCAAAATGCGATAAACCAAAGGCTTTACGCTTCTTCAATTGTAATGATCTTCAGTTAAGTGGACTCACACACGTTGATAGTCCAAAAGGGCATATTAGCATACTAAACTGCACCAATGTTATAGTATCCAATCTTCATATCATTGCACCAGATGATAGTGAAAACACAGATGGCATTGATATCAGTAAGTCAAGACATGTCAATATTCATGACTGCATAATTGGAACAGGAGACGATTGCATTGCTATTAAAGGTGGCTCAGTCCTTGTCAATGTCGCCAACATTGCTTGCGGACCGGGTCATGGAATTAGTGTGGGAAGCATAGGTGAAGAAGGAGCTTATGAGACAGTCGCAGAGGTTTATGTACGAAATTGTACCTTCAATGGTACGATGAATGGAGCTAGAATCAAGACATGGCCGGGTGGTTCTGGATATGCCAAGAATATCTTGTATGAGAAAATCACTCTAATAGGAGTTCAAAATCCTATTATTATTGATCAAAACTACTGCAATGGTGAACGTGACTGCGGAGATATAACGTCGTCGAATTCAGCAGTGGAGGTGAGTAATGTGACATTCAATGACTTTCGAGGAACTCTGGCGGATGATCATGCGATTACATTTGATTGCTCCAAGACTGCAGATGGCACTCTAGCTTGTCTCAACATTAATATGAAGCAAATCAACATTACTTCCATAGATCCCGAAAAGGAAATCTCCGTCTTATGCAAGAATGTAAATGGAACTACTGAAGATACTAGTCCCAGTGTTCATTGCCTACAAGCTAGAGATTCAACCATTGATGCAGATAGTCCTGACGCATATAGTCCCGATCCCTACGCCTTTTCCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

429

Amino Acids

46.36

Weight (kDa)

4.67

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 50 - 392 4.8e-82 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 277
AciI CCGC 3 cut(s) 726, 963, 1037
AclWI GGATC 2 cut(s) 1130, 1262
AcoI YGGCCR 2 cut(s) 100, 851
AcsI RAATTY 2 cut(s) 215, 982
AcuI CTGAAG 3 cut(s) 138, 470, 1206
AfaI GTAC 3 cut(s) 801, 811, 823
AfiI CCNNNNNNNGG 1 cut(s) 737
AflIII ACRYGT 2 cut(s) 505, 631
AgsI TTSAA 6 cut(s) 130, 472, 817, 914, 1015, 1230
AhlI ACTAGT 1 cut(s) 1193
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 6 cut(s) 21, 122, 773, 836, 1091, 1220
AluI AGCT 6 cut(s) 21, 122, 773, 836, 1091, 1220
Alw26I GTCTC 3 cut(s) 660, 773, 1100
AlwI GGATC 2 cut(s) 1130, 1262
AoxI GGCC 2 cut(s) 100, 851
ApeKI GCWGC 2 cut(s) 152, 325
ApoI RAATTY 2 cut(s) 215, 982
AseI ATTAAT 2 cut(s) 357, 1104
AspS9I GGNCC 2 cut(s) 224, 728
AsuC2I CCSGG 2 cut(s) 732, 855
AsuHPI GGTGA 4 cut(s) 352, 773, 962, 1009
AvaII GGWCC 2 cut(s) 224, 728
BalI TGGCCA 1 cut(s) 102
BanI GGYRCC 1 cut(s) 277
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
BbvI GCAGC 2 cut(s) 164, 312
BccI CCATC 3 cut(s) 92, 599, 1073
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 1 cut(s) 322
BciVI GTATCC 1 cut(s) 571
BclI TGATCA 3 cut(s) 351, 931, 1042
BcnI CCSGG 2 cut(s) 732, 855
BcoDI GTCTC 3 cut(s) 660, 773, 1100
BcuI ACTAGT 1 cut(s) 1193
BfaI CTAG 4 cut(s) 837, 1088, 1194, 1221
BfmI CTRYAG 1 cut(s) 1074
BfuI GTATCC 1 cut(s) 571
BisI GCNGC 2 cut(s) 153, 326
BlsI GCNGC 2 cut(s) 154, 327
Bme1390I CCNGG 3 cut(s) 322, 732, 855
Bme18I GGWCC 2 cut(s) 224, 728
BmgT120I GGNCC 2 cut(s) 224, 728
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 3 cut(s) 322, 732, 855
BmrI ACTGGG 1 cut(s) 1194
BmsI GCATC 1 cut(s) 1228
BmuI ACTGGG 1 cut(s) 1194
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 732, 855
Bsa29I ATCGAT 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse1I ACTGG 1 cut(s) 1200
Bse3DI GCAATG 5 cut(s) 576, 675, 717, 952, 1207
BseBI CCWGG 1 cut(s) 322
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 1045
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMI GCAATG 5 cut(s) 576, 675, 717, 952, 1207
BseMII CTCAG 2 cut(s) 234, 708
BseNI ACTGG 1 cut(s) 1200
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 164, 312
BsgI GTGCAG 2 cut(s) 171, 529
BshFI GGCC 2 cut(s) 102, 853
BshNI GGYRCC 1 cut(s) 277
BshVI ATCGAT 1 cut(s) 249
BsiSI CCGG 2 cut(s) 731, 854
BslFI GGGAC 2 cut(s) 1182, 1248
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 3 cut(s) 660, 773, 1100
BsmBI CGTCTC 1 cut(s) 660
BsmFI GGGAC 2 cut(s) 1182, 1248
BsnI GGCC 2 cut(s) 102, 853
Bsp143I GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
BspACI CCGC 3 cut(s) 726, 963, 1037
BspANI GGCC 2 cut(s) 102, 853
BspCNI CTCAG 2 cut(s) 233, 707
BspDI ATCGAT 1 cut(s) 249
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 1078
BspPI GGATC 2 cut(s) 1130, 1262
BspT107I GGYRCC 1 cut(s) 277
BsrDI GCAATG 5 cut(s) 576, 675, 717, 952, 1207
BsrI ACTGG 1 cut(s) 1200
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
BssT1I CCWWGG 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 86, 784
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 19, 724
BstDEI CTNAG 2 cut(s) 220, 694
BstF5I GGATG 1 cut(s) 1045
BstKTI GATC 6 cut(s) 354, 484, 934, 1045, 1138, 1270
BstMAI GTCTC 3 cut(s) 660, 773, 1100
BstMBI GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
BstMWI GCNNNNNNNGC 3 cut(s) 140, 149, 719
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 3 cut(s) 320, 730, 853
BstSFI CTRYAG 1 cut(s) 1074
BstV1I GCAGC 2 cut(s) 164, 312
BstX2I RGATCY 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 1135
Bsu15I ATCGAT 1 cut(s) 249
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 2 cut(s) 102, 853
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 1 cut(s) 1045
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 2 cut(s) 996, 1207
Cac8I GCNNGC 2 cut(s) 19, 724
CciI TCATGA 1 cut(s) 643
Cfr13I GGNCC 2 cut(s) 224, 728
ClaI ATCGAT 1 cut(s) 249
CpoI CGGWCCG 1 cut(s) 728
CseI GACGC 1 cut(s) 1262
Csp6I GTAC 3 cut(s) 800, 810, 822
CspI CGGWCCG 1 cut(s) 728
CviAII CATG 6 cut(s) 135, 632, 644, 737, 849, 1046
CviQI GTAC 3 cut(s) 800, 810, 822
DdeI CTNAG 2 cut(s) 220, 694
DpnI GATC 6 cut(s) 353, 483, 933, 1044, 1137, 1269
DpnII GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
EaeI YGGCCR 2 cut(s) 100, 851
EciI GGCGGA 1 cut(s) 1052
Eco130I CCWWGG 1 cut(s) 398
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 224, 728
Eco57I CTGAAG 3 cut(s) 138, 470, 1206
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 1 cut(s) 320
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 398
Esp3I CGTCTC 1 cut(s) 660
FaeI CATG 6 cut(s) 138, 635, 647, 740, 852, 1049
FalI AAGNNNNNCTT 2 cut(s) 866, 898
FaqI GGGAC 2 cut(s) 1182, 1248
FatI CATG 6 cut(s) 134, 631, 643, 736, 848, 1045
FbaI TGATCA 3 cut(s) 351, 931, 1042
Fnu4HI GCNGC 2 cut(s) 153, 326
FokI GGATG 1 cut(s) 1052
Fsp4HI GCNGC 2 cut(s) 153, 326
FspBI CTAG 4 cut(s) 837, 1088, 1194, 1221
GluI GCNGC 2 cut(s) 153, 326
HaeIII GGCC 2 cut(s) 102, 853
HapII CCGG 2 cut(s) 731, 854
HgaI GACGC 1 cut(s) 1262
Hin1II CATG 6 cut(s) 138, 635, 647, 740, 852, 1049
HindIII AAGCTT 2 cut(s) 19, 120
HinfI GANTC 5 cut(s) 113, 317, 498, 840, 1226
HpaII CCGG 2 cut(s) 731, 854
HphI GGTGA 4 cut(s) 352, 773, 962, 1009
Hpy166II GTNNAC 5 cut(s) 343, 395, 405, 497, 953
Hpy188I TCNGA 3 cut(s) 118, 337, 424
Hpy188III TCNNGA 9 cut(s) 127, 293, 627, 644, 844, 864, 1139, 1250, 1265
Hpy8I GTNNAC 5 cut(s) 343, 395, 405, 497, 953
Hpy99I CGWCG 2 cut(s) 979, 982
HpyAV CCTTC 2 cut(s) 761, 823
HpyCH4III ACNGT 2 cut(s) 86, 784
HpyCH4IV ACGT 3 cut(s) 507, 955, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 149, 719
HpyF3I CTNAG 2 cut(s) 220, 694
HpySE526I ACGT 3 cut(s) 507, 955, 974
Hsp92II CATG 6 cut(s) 138, 635, 647, 740, 852, 1049
Ksp22I TGATCA 3 cut(s) 351, 931, 1042
Kzo9I GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
LmnI GCTCC 3 cut(s) 770, 833, 1070
Lsp1109I GCAGC 2 cut(s) 164, 312
LweI GCATC 1 cut(s) 1228
MaeI CTAG 4 cut(s) 837, 1088, 1194, 1221
MaeII ACGT 3 cut(s) 507, 955, 974
MaeIII GTNAC 2 cut(s) 956, 1006
MalI GATC 6 cut(s) 353, 483, 933, 1044, 1137, 1269
MboI GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
MboII GAAGA 5 cut(s) 460, 476, 560, 776, 1199
MfeI CAATTG 1 cut(s) 472
MflI RGATCY 1 cut(s) 1135
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 7 cut(s) 215, 270, 472, 654, 741, 805, 982
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 1 cut(s) 492
MmeI TCCRAC 2 cut(s) 73, 402
MnlI CCTC 5 cut(s) 300, 331, 784, 988, 1019
Mox20I TGGCCA 1 cut(s) 102
MscI TGGCCA 1 cut(s) 102
MseI TTAA 5 cut(s) 69, 357, 491, 684, 1104
MslI CAYNNNNRTG 1 cut(s) 1106
Msp20I TGGCCA 1 cut(s) 102
MspI CCGG 2 cut(s) 731, 854
MspR9I CCNGG 3 cut(s) 322, 732, 855
MunI CAATTG 1 cut(s) 472
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 140, 149, 719
NciI CCSGG 2 cut(s) 732, 855
NdeII GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
NlaIII CATG 6 cut(s) 138, 635, 647, 740, 852, 1049
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 2 cut(s) 956, 1006
NspI RCATGY 1 cut(s) 635
PagI TCATGA 1 cut(s) 643
PciI ACATGT 1 cut(s) 631
PfeI GAWTC 4 cut(s) 113, 317, 840, 1226
PflFI GACNNNGTC 1 cut(s) 732
PkrI GCNGC 2 cut(s) 154, 327
PleI GAGTC 1 cut(s) 492
PpsI GAGTC 1 cut(s) 492
PpuMI RGGWCCY 1 cut(s) 224
PscI ACATGT 1 cut(s) 631
PshBI ATTAAT 2 cut(s) 357, 1104
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 2 cut(s) 224, 728
PspPPI RGGWCCY 1 cut(s) 224
PsrI GAACNNNNNNTAC 2 cut(s) 162, 194
PstI CTGCAG 1 cut(s) 1078
PsuI RGATCY 1 cut(s) 1135
PsyI GACNNNGTC 1 cut(s) 732
RsaI GTAC 3 cut(s) 801, 811, 823
RsaNI GTAC 3 cut(s) 800, 810, 822
RseI CAYNNNNRTG 1 cut(s) 1106
Rsr2I CGGWCCG 1 cut(s) 728
RsrII CGGWCCG 1 cut(s) 728
SaqAI TTAA 5 cut(s) 69, 357, 491, 684, 1104
SatI GCNGC 2 cut(s) 153, 326
Sau3AI GATC 6 cut(s) 351, 481, 931, 1042, 1135, 1267
Sau96I GGNCC 2 cut(s) 224, 728
SchI GAGTC 1 cut(s) 492
ScrFI CCNGG 3 cut(s) 322, 732, 855
SfaNI GCATC 1 cut(s) 1228
SfcI CTRYAG 1 cut(s) 1074
SinI GGWCC 2 cut(s) 224, 728
SmiMI CAYNNNNRTG 1 cut(s) 1106
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
SpeI ACTAGT 1 cut(s) 1193
Sse9I AATT 7 cut(s) 215, 270, 472, 654, 741, 805, 982
SsiI CCGC 3 cut(s) 726, 963, 1037
SspI AATATT 1 cut(s) 640
SspMI CTAG 4 cut(s) 837, 1088, 1194, 1221
StyD4I CCNGG 3 cut(s) 320, 730, 853
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 86, 784
TaiI ACGT 3 cut(s) 510, 958, 977
TaqI TCGA 3 cut(s) 249, 980, 1024
TasI AATT 7 cut(s) 215, 270, 472, 654, 741, 805, 982
TfiI GAWTC 4 cut(s) 113, 317, 840, 1226
Tru1I TTAA 5 cut(s) 69, 357, 491, 684, 1104
Tru9I TTAA 5 cut(s) 69, 357, 491, 684, 1104
TscAI CASTG 2 cut(s) 996, 1207
TseFI GTSAC 2 cut(s) 956, 1006
TseI GCWGC 2 cut(s) 152, 325
Tsp45I GTSAC 2 cut(s) 956, 1006
TspDTI ATGAA 6 cut(s) 381, 560, 632, 842, 1124, 1196
TspGWI ACGGA 1 cut(s) 1144
TspRI CASTG 2 cut(s) 996, 1207
Tth111I GACNNNGTC 1 cut(s) 732
VpaK11BI GGWCC 2 cut(s) 224, 728
VspI ATTAAT 2 cut(s) 357, 1104
XapI RAATTY 2 cut(s) 215, 982
XceI RCATGY 1 cut(s) 635
XspI CTAG 4 cut(s) 837, 1088, 1194, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.