Rw3G021790

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000579
Physical Location & Seq
Reverse (-)
26427801 .. 26431018
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021790.1

Sequence Viewer

Length: 1308 bp
ATGCACGAGTCAATTGTATTAAGTATAAGGGAGCAGCTTCCAACTATGAGAAAACTACTTGCAAGCTTTTTGATAGTTTGCATTTTTACTACATCGTCAAATTTTAATATTGGGTATTGCCAAGAAACTTTTAATGTGCTTGACCACGGTGCTGTTGGAGATGGCCAAACCGATGATTCTGAAGCTTTCTTGAAAGCATGGAGTGCTTTATGTGCAGCAAAAGGAACACCAGCGCTTGTAATACCAGTGGAAAAAACATTCCTTTTGCAACCTACAAAATTCTCAGGTCCTTGCATATCCAATGGCTCTATCTATGTTGAGATTTTGGGGAAAATTGTGGCACCCAAAACTCCTCAAGAATGGAAAAAGTGTGAAAAAAAATCCTGGCTGCTCTTTTCAGAGGTGAACAACCTGATCATTAATGGAAATGAAACAGGAGTAATCAATGGGAATGGTTTACCTTGGTGGACAGAGGCTCTACGCTTCTTCAATTGTAATGATCTTCAGTTAAGTGGACTCACACACGTTGATAGTCCAAAAGGGCATATTAGCATACTAAACTGCACCAATGTTATCGTATCCAATCTTCATATCATTGCACCAGATGATAGTGAAAACACAGATGGCATTGATATCAATATGTCAAGACATGTCAATATTCATGACTGCATAATTGGAACAGGAGACGATTGCATTGCTATTAAACGTGGCTCAGTCCTTGTATATGTCACCAACATTGCTTGTGGACCAGGTCATGGAATTAGTGTGGGAAGCATAGGTGAAATAGAAGGAGCTTATGAGACAGTCGCAGAGGTTTATGTACGAAATTGTACCTTCAATGGTACGATGAATGGAGCTAGAATCAAGACATGGCCCGGTGGTTCTGGATATGCCAAGAATATCTCGTATGAGAAAATCACTCTAATAGGAGTTCAAAATCCTATTATTATTGATCAAAACTACTGCAATGGTAAACACGACTGCGGTAATATAACGTCGAATTCAGCAGTGGAGGTGAGTAATGTGATATTCAACGACTTTAGAGGAACTCTGGCGGATGAACATGCGATTACATTTGATTGCTCCAAGACTGCATATGGCACTCTAGCTTGTCTCAACATTAATATGGAGCAAATCAACATTACTTCCATAGATCCCGAAAAGGAAATCTCCGTCTTATGCAAGAATGTAAATGGAACTACTGAAGATACCAGTCCCAGTGTTCATTGCCTACAAGCTAGAAATTCAACCATTGATGCAGATAGTCCTGATGCAGATAGTCCTGATCCCTACGCCTATTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

435

Amino Acids

47.18

Weight (kDa)

4.92

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 44 - 256 9.5e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 71 - 398 5.6e-72 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 340
AccB7I CCANNNNNTGG 1 cut(s) 755
AciI CCGC 2 cut(s) 984, 1055
AclWI GGATC 2 cut(s) 1148, 1280
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 4 cut(s) 100, 278, 1000, 1243
AcuI CTGAAG 3 cut(s) 201, 488, 1224
AfaI GTAC 3 cut(s) 822, 832, 844
AfeI AGCGCT 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 755
AflIII ACRYGT 2 cut(s) 523, 649
AgsI TTSAA 6 cut(s) 193, 490, 838, 935, 1033, 1248
AjnI CCWGG 2 cut(s) 383, 748
AluBI AGCT 7 cut(s) 37, 66, 185, 794, 857, 1109, 1238
AluI AGCT 7 cut(s) 37, 66, 185, 794, 857, 1109, 1238
Alw26I GTCTC 3 cut(s) 678, 794, 1118
AlwI GGATC 2 cut(s) 1148, 1280
Aor51HI AGCGCT 1 cut(s) 234
AoxI GGCC 2 cut(s) 163, 872
ApeKI GCWGC 3 cut(s) 34, 215, 388
ApoI RAATTY 4 cut(s) 100, 278, 1000, 1243
AseI ATTAAT 2 cut(s) 420, 1122
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 3 cut(s) 287, 746, 873
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 4 cut(s) 415, 721, 791, 1027
AvaII GGWCC 2 cut(s) 287, 746
BalI TGGCCA 1 cut(s) 165
BanI GGYRCC 1 cut(s) 340
BarI GAAGNNNNNNTAC 2 cut(s) 570, 602
BauI CACGAG 1 cut(s) 5
BbvI GCAGC 3 cut(s) 46, 227, 375
BccI CCATC 2 cut(s) 155, 617
BcgI CGANNNNNNTGC 2 cut(s) 677, 711
BciT130I CCWGG 2 cut(s) 385, 750
BciVI GTATCC 1 cut(s) 589
BclI TGATCA 2 cut(s) 414, 952
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 678, 794, 1118
BfaI CTAG 3 cut(s) 858, 1106, 1239
BfoI RGCGCY 1 cut(s) 236
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 3 cut(s) 35, 216, 389
BlsI GCNGC 3 cut(s) 36, 217, 390
Bme1390I CCNGG 3 cut(s) 385, 750, 876
Bme18I GGWCC 2 cut(s) 287, 746
BmgT120I GGNCC 3 cut(s) 287, 746, 873
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 3 cut(s) 385, 750, 876
BmrI ACTGGG 1 cut(s) 1212
BmsI GCATC 2 cut(s) 1246, 1261
BmuI ACTGGG 1 cut(s) 1212
BpuEI CTTGAG 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 876
BsaJI CCNNGG 2 cut(s) 145, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 3 cut(s) 245, 1212, 1218
Bse3DI GCAATG 5 cut(s) 594, 693, 735, 973, 1225
BseBI CCWGG 2 cut(s) 385, 750
BseDI CCNNGG 2 cut(s) 145, 461
BseGI GGATG 1 cut(s) 1063
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 5 cut(s) 594, 693, 735, 973, 1225
BseMII CTCAG 2 cut(s) 297, 726
BseNI ACTGG 3 cut(s) 245, 1212, 1218
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 3 cut(s) 46, 227, 375
BsgI GTGCAG 2 cut(s) 234, 547
BshFI GGCC 2 cut(s) 165, 874
BshNI GGYRCC 1 cut(s) 340
BsiSI CCGG 1 cut(s) 876
BslFI GGGAC 1 cut(s) 1200
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 3 cut(s) 678, 794, 1118
BsmBI CGTCTC 1 cut(s) 678
BsmFI GGGAC 1 cut(s) 1200
BsnI GGCC 2 cut(s) 165, 874
Bsp143I GATC 5 cut(s) 414, 499, 952, 1153, 1285
BspACI CCGC 2 cut(s) 984, 1055
BspANI GGCC 2 cut(s) 165, 874
BspCNI CTCAG 2 cut(s) 296, 725
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 2 cut(s) 1148, 1280
BspT107I GGYRCC 1 cut(s) 340
BsrDI GCAATG 5 cut(s) 594, 693, 735, 973, 1225
BsrI ACTGG 3 cut(s) 245, 1212, 1218
BssECI CCNNGG 2 cut(s) 145, 461
BssMI GATC 5 cut(s) 414, 499, 952, 1153, 1285
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 385, 750
Bst4CI ACNGT 2 cut(s) 149, 805
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 283, 712
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 1063
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 5 cut(s) 417, 502, 955, 1156, 1288
BstMAI GTCTC 3 cut(s) 678, 794, 1118
BstMBI GATC 5 cut(s) 414, 499, 952, 1153, 1285
BstMWI GCNNNNNNNGC 2 cut(s) 203, 212
BstNI CCWGG 2 cut(s) 385, 750
BstNSI RCATGY 2 cut(s) 653, 1067
BstSCI CCNGG 3 cut(s) 383, 748, 874
BstV1I GCAGC 3 cut(s) 46, 227, 375
BstX2I RGATCY 1 cut(s) 1153
BstYI RGATCY 1 cut(s) 1153
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 2 cut(s) 165, 874
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 1063
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 3 cut(s) 252, 1014, 1225
Cac8I GCNNGC 1 cut(s) 64
CciI TCATGA 1 cut(s) 661
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 3 cut(s) 287, 746, 873
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 3 cut(s) 821, 831, 843
CviAII CATG 6 cut(s) 198, 650, 662, 755, 870, 1064
CviQI GTAC 3 cut(s) 821, 831, 843
DdeI CTNAG 2 cut(s) 283, 712
DpnI GATC 5 cut(s) 416, 501, 954, 1155, 1287
DpnII GATC 5 cut(s) 414, 499, 952, 1153, 1285
EaeI YGGCCR 1 cut(s) 163
EciI GGCGGA 1 cut(s) 1070
Eco130I CCWWGG 1 cut(s) 461
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 287, 746
Eco47III AGCGCT 1 cut(s) 234
Eco57I CTGAAG 3 cut(s) 201, 488, 1224
EcoO109I RGGNCCY 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 1000
EcoRII CCWGG 2 cut(s) 383, 748
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 6 cut(s) 201, 653, 665, 758, 873, 1067
FaqI GGGAC 1 cut(s) 1200
FatI CATG 6 cut(s) 197, 649, 661, 754, 869, 1063
FauNDI CATATG 1 cut(s) 1096
FbaI TGATCA 2 cut(s) 414, 952
Fnu4HI GCNGC 3 cut(s) 35, 216, 389
FokI GGATG 1 cut(s) 1070
Fsp4HI GCNGC 3 cut(s) 35, 216, 389
FspBI CTAG 3 cut(s) 858, 1106, 1239
GlaI GCGC 1 cut(s) 234
GluI GCNGC 3 cut(s) 35, 216, 389
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 2 cut(s) 165, 874
HapII CCGG 1 cut(s) 876
HhaI GCGC 1 cut(s) 235
Hin1II CATG 6 cut(s) 201, 653, 665, 758, 873, 1067
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 64, 183
HinfI GANTC 4 cut(s) 8, 176, 516, 861
HpaII CCGG 1 cut(s) 876
HphI GGTGA 4 cut(s) 415, 721, 791, 1027
Hpy166II GTNNAC 6 cut(s) 406, 458, 468, 515, 746, 974
Hpy188I TCNGA 2 cut(s) 181, 400
Hpy188III TCNNGA 9 cut(s) 190, 356, 645, 662, 865, 885, 1157, 1268, 1283
Hpy8I GTNNAC 6 cut(s) 406, 458, 468, 515, 746, 974
Hpy99I CGWCG 1 cut(s) 1000
HpyAV CCTTC 2 cut(s) 782, 844
HpyCH4III ACNGT 2 cut(s) 149, 805
HpyCH4IV ACGT 3 cut(s) 525, 706, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 212
HpyF3I CTNAG 2 cut(s) 283, 712
HpySE526I ACGT 3 cut(s) 525, 706, 995
Hsp92II CATG 6 cut(s) 201, 653, 665, 758, 873, 1067
HspAI GCGC 1 cut(s) 233
Ksp22I TGATCA 2 cut(s) 414, 952
Kzo9I GATC 5 cut(s) 414, 499, 952, 1153, 1285
LmnI GCTCC 5 cut(s) 31, 791, 854, 1088, 1129
Lsp1109I GCAGC 3 cut(s) 46, 227, 375
LweI GCATC 2 cut(s) 1246, 1261
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 3 cut(s) 858, 1106, 1239
MaeII ACGT 3 cut(s) 525, 706, 995
MaeIII GTNAC 1 cut(s) 727
MalI GATC 5 cut(s) 416, 501, 954, 1155, 1287
MboI GATC 5 cut(s) 414, 499, 952, 1153, 1285
MboII GAAGA 4 cut(s) 478, 494, 578, 1217
MfeI CAATTG 2 cut(s) 12, 490
MflI RGATCY 1 cut(s) 1153
MlsI TGGCCA 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 165
MlyI GAGTC 2 cut(s) 17, 510
MmeI TCCRAC 2 cut(s) 65, 136
MnlI CCTC 6 cut(s) 363, 394, 466, 805, 1006, 1037
Mox20I TGGCCA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 165
MseI TTAA 7 cut(s) 20, 105, 132, 420, 509, 702, 1122
MslI CAYNNNNRTG 1 cut(s) 1124
Msp20I TGGCCA 1 cut(s) 165
MspI CCGG 1 cut(s) 876
MspR9I CCNGG 3 cut(s) 385, 750, 876
MunI CAATTG 2 cut(s) 12, 490
MvaI CCWGG 2 cut(s) 385, 750
MwoI GCNNNNNNNGC 2 cut(s) 203, 212
NciI CCSGG 1 cut(s) 876
NdeI CATATG 1 cut(s) 1096
NdeII GATC 5 cut(s) 414, 499, 952, 1153, 1285
NlaIII CATG 6 cut(s) 201, 653, 665, 758, 873, 1067
NlaIV GGNNCC 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 727
NspI RCATGY 2 cut(s) 653, 1067
PagI TCATGA 1 cut(s) 661
PciI ACATGT 1 cut(s) 649
PfeI GAWTC 2 cut(s) 176, 861
PflFI GACNNNGTC 1 cut(s) 750
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 3 cut(s) 36, 217, 390
PleI GAGTC 2 cut(s) 16, 510
PpsI GAGTC 2 cut(s) 16, 510
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 649
PshBI ATTAAT 2 cut(s) 420, 1122
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 383, 748
PspGI CCWGG 2 cut(s) 383, 748
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 3 cut(s) 287, 746, 873
PspPPI RGGWCCY 1 cut(s) 287
PsuI RGATCY 1 cut(s) 1153
PsyI GACNNNGTC 1 cut(s) 750
RsaI GTAC 3 cut(s) 822, 832, 844
RsaNI GTAC 3 cut(s) 821, 831, 843
RseI CAYNNNNRTG 1 cut(s) 1124
SaqAI TTAA 7 cut(s) 20, 105, 132, 420, 509, 702, 1122
SatI GCNGC 3 cut(s) 35, 216, 389
Sau3AI GATC 5 cut(s) 414, 499, 952, 1153, 1285
Sau96I GGNCC 3 cut(s) 287, 746, 873
SchI GAGTC 2 cut(s) 17, 510
ScrFI CCNGG 3 cut(s) 385, 750, 876
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 2 cut(s) 1246, 1261
SinI GGWCC 2 cut(s) 287, 746
SmiMI CAYNNNNRTG 1 cut(s) 1124
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
SsiI CCGC 2 cut(s) 984, 1055
SspI AATATT 2 cut(s) 109, 658
SspMI CTAG 3 cut(s) 858, 1106, 1239
StyD4I CCNGG 3 cut(s) 383, 748, 874
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 2 cut(s) 149, 805
TaiI ACGT 3 cut(s) 528, 709, 998
TaqI TCGA 1 cut(s) 998
TfiI GAWTC 2 cut(s) 176, 861
Tru1I TTAA 7 cut(s) 20, 105, 132, 420, 509, 702, 1122
Tru9I TTAA 7 cut(s) 20, 105, 132, 420, 509, 702, 1122
TscAI CASTG 3 cut(s) 252, 1014, 1225
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 3 cut(s) 34, 215, 388
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 6 cut(s) 444, 578, 650, 863, 1074, 1214
TspGWI ACGGA 1 cut(s) 1162
TspRI CASTG 3 cut(s) 252, 1014, 1225
Tth111I GACNNNGTC 1 cut(s) 750
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 2 cut(s) 287, 746
VspI ATTAAT 2 cut(s) 420, 1122
XapI RAATTY 4 cut(s) 100, 278, 1000, 1243
XceI RCATGY 2 cut(s) 653, 1067
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XspI CTAG 3 cut(s) 858, 1106, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.