FvH4_6g35100

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000579
Physical Location & Seq
Reverse (-)
27738681 .. 27741561
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35100.t1

Sequence Viewer

Length: 1215 bp
ATGAGAAGTGTTCTTGTGAGCTGTGTGTTACTTTGTATTCTTGCTTGGTGTGATTTTGAAATTGGTTATGGCCAAATGACTTATAATGTACTCGACCATGGTGCGGTTGGAGATGGCCACAGAGATGATTCTGAAGCTTTTTTGAAAGCATGGAACGCCTCATGTCTAGATAAAGGGACACCAAGACTTGTAATACCAGGGGAGAAGACCTATCTTCTTCAACCTACGAACTTCTCAGGTCCTTGCCAGTCCAGCTCCATTCACGTTGAGATTTCTGGAAAACTTCTGGCACCCAGTCCAGATGCATGGAAAGAGTGTGTAGACCTTTGGTTAACCTTTTCAGAGGTGACCAACCTCACCGTAAGTGGATCAGGAGAAATCAACGGCCAAGGTTCCCTTTGGTGGAATAAATATGACCTGCTTAACTATGTGAATAATTATAAGGTGCAATGCGAACGACCAAAGGCTTTACGTTTCTACAGATGCGATAGTCTTCAATTAAGTGGACTTACTCTAGCTGACAGTCCAAGAACACACATCTCCATTCAGTATTGCAATTATGCGAATGTCTCGTACCTTCAGATTAGGGCACCTGAAGATAGTCCCAACACAGATGGAATTGACATCTCTATGTCAAGCAATGTCAATATCCATGATTGTAATATTGGAACAGGTGATGATTGTATCGCTGTTAACAATGGAACATTCATTATCGATATCAAGAACATCGCTTGCGGACCAGGTCATGGAATTAGCATTGGAAGTTTGGGCAAGAATGGAGATCATAACACAGTAGAAAACATACATGTAAAGAATTGCAGTTTCAATAGCACAATGAATGGAGCCAGAATTAAGACATTTCAGGGTGGTTCTGGATATGCCAGAAGGATATCATTTGAAGAAATCACTCTTACAACTGCTGGGCACCCCATTATCATTGATCAATACTACTGTCCTGAACATGACTGCGAAAATTCGACTTCGTCCGTACAGGTGAGTGATGTGACATTCAGTGGCTTCCATGGAACTTCTAAGAATGAGCAAGCAATTACATTGAACTGCAGCCAGAGTGTTAATTGTGTAAACATTGTGATGAACAATATCAATATCACCTCAGCAGTTGCCAGTGAGGAGGTGAGGGCTTCTTGCAGCTATGTAAATGGGACTTCTTACGCAACTGTACCACAAGTCCCTTGCCTGAGCTCTAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

405

Amino Acids

43.94

Weight (kDa)

5.24

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 387 5.1e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 84, 441
Acc36I ACCTGC 1 cut(s) 426
AccB1I GGYRCC 3 cut(s) 289, 589, 924
AccB7I CCANNNNNTGG 1 cut(s) 746
AccI GTMKAC 1 cut(s) 321
AciI CCGC 2 cut(s) 104, 735
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 3 cut(s) 70, 115, 385
AcsI RAATTY 1 cut(s) 973
AcuI CTGAAG 3 cut(s) 153, 563, 615
AfaI GTAC 4 cut(s) 90, 575, 990, 1182
AfiI CCNNNNNNNGG 3 cut(s) 103, 402, 746
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 7 cut(s) 59, 145, 221, 497, 826, 899, 1057
AjnI CCWGG 2 cut(s) 196, 739
AluBI AGCT 6 cut(s) 21, 137, 255, 518, 1152, 1203
AluI AGCT 6 cut(s) 21, 137, 255, 518, 1152, 1203
Alw21I GWGCWC 1 cut(s) 1205
Alw26I GTCTC 1 cut(s) 574
AlwI GGATC 1 cut(s) 376
AoxI GGCC 3 cut(s) 70, 115, 385
ApeKI GCWGC 2 cut(s) 1062, 1149
ApoI RAATTY 1 cut(s) 973
AspS9I GGNCC 2 cut(s) 239, 737
AsuHPI GGTGA 6 cut(s) 349, 358, 686, 1006, 1102, 1147
AvaII GGWCC 2 cut(s) 239, 737
BaeGI GKGCMC 2 cut(s) 592, 927
BalI TGGCCA 2 cut(s) 72, 117
BanI GGYRCC 3 cut(s) 289, 589, 924
BanII GRGCYC 1 cut(s) 1205
BbsI GAAGAC 2 cut(s) 212, 485
Bbv12I GWGCWC 1 cut(s) 1205
BbvCI CCTCAGC 1 cut(s) 1114
BbvI GCAGC 2 cut(s) 1074, 1161
BccI CCATC 2 cut(s) 107, 608
BceAI ACGGC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 198, 741
BclI TGATCA 1 cut(s) 940
BcoDI GTCTC 1 cut(s) 574
BfaI CTAG 2 cut(s) 167, 515
BfmI CTRYAG 2 cut(s) 478, 1060
BfuAI ACCTGC 1 cut(s) 426
BisI GCNGC 2 cut(s) 1063, 1150
BlsI GCNGC 2 cut(s) 1064, 1151
Bme1390I CCNGG 2 cut(s) 198, 741
Bme18I GGWCC 2 cut(s) 239, 737
BmgT120I GGNCC 2 cut(s) 239, 737
BmiI GGNNCC 5 cut(s) 291, 394, 591, 844, 926
BmrFI CCNGG 2 cut(s) 198, 741
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 2 cut(s) 292, 473
BmuI ACTGGG 1 cut(s) 288
BpiI GAAGAC 2 cut(s) 212, 485
Bpu10I CCTNAGC 2 cut(s) 1114, 1199
Bsa29I ATCGAT 1 cut(s) 714
BsaJI CCNNGG 4 cut(s) 97, 197, 388, 1021
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 402, 746
Bse1I ACTGG 3 cut(s) 247, 294, 1125
Bse3DI GCAATG 2 cut(s) 455, 646
BseBI CCWGG 2 cut(s) 198, 741
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 4 cut(s) 97, 197, 388, 1021
BseLI CCNNNNNNNGG 3 cut(s) 103, 402, 746
BseMI GCAATG 2 cut(s) 455, 646
BseMII CTCAG 3 cut(s) 249, 1128, 1190
BseNI ACTGG 3 cut(s) 247, 294, 1125
BseRI GAGGAG 1 cut(s) 1145
BseSI GKGCMC 2 cut(s) 592, 927
BseXI GCAGC 2 cut(s) 1074, 1161
BseYI CCCAGC 1 cut(s) 920
BshFI GGCC 3 cut(s) 72, 117, 387
BshNI GGYRCC 3 cut(s) 289, 589, 924
BshVI ATCGAT 1 cut(s) 714
BsiHKAI GWGCWC 1 cut(s) 1205
BslFI GGGAC 4 cut(s) 190, 588, 1175, 1177
BslI CCNNNNNNNGG 3 cut(s) 103, 402, 746
BsmAI GTCTC 1 cut(s) 574
BsmFI GGGAC 4 cut(s) 190, 588, 1175, 1177
BsnI GGCC 3 cut(s) 72, 117, 387
Bsp1286I GDGCHC 3 cut(s) 592, 927, 1205
Bsp143I GATC 3 cut(s) 368, 781, 940
Bsp19I CCATGG 2 cut(s) 97, 1021
BspACI CCGC 2 cut(s) 104, 735
BspANI GGCC 3 cut(s) 72, 117, 387
BspCNI CTCAG 3 cut(s) 248, 1127, 1191
BspDI ATCGAT 1 cut(s) 714
BspLI GGNNCC 5 cut(s) 291, 394, 591, 844, 926
BspMAI CTGCAG 1 cut(s) 1064
BspMI ACCTGC 1 cut(s) 426
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 3 cut(s) 289, 589, 924
BsrDI GCAATG 2 cut(s) 455, 646
BsrI ACTGG 3 cut(s) 247, 294, 1125
BssECI CCNNGG 4 cut(s) 97, 197, 388, 1021
BssMI GATC 3 cut(s) 368, 781, 940
BssT1I CCWWGG 3 cut(s) 97, 388, 1021
Bst2UI CCWGG 2 cut(s) 198, 741
Bst4CI ACNGT 5 cut(s) 361, 524, 793, 953, 1180
BstC8I GCNNGC 2 cut(s) 733, 1044
BstDEI CTNAG 4 cut(s) 235, 1032, 1114, 1199
BstDSI CCRYGG 2 cut(s) 97, 1021
BstEII GGTNACC 1 cut(s) 346
BstKTI GATC 3 cut(s) 371, 784, 943
BstMAI GTCTC 1 cut(s) 574
BstMBI GATC 3 cut(s) 368, 781, 940
BstMWI GCNNNNNNNGC 2 cut(s) 155, 252
BstNI CCWGG 2 cut(s) 198, 741
BstNSI RCATGY 1 cut(s) 809
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 196, 739
BstSFI CTRYAG 2 cut(s) 478, 1060
BstSLI GKGCMC 2 cut(s) 592, 927
BstV1I GCAGC 2 cut(s) 1074, 1161
BstV2I GAAGAC 2 cut(s) 212, 485
BstXI CCANNNNNNTGG 1 cut(s) 306
Bsu15I ATCGAT 1 cut(s) 714
BsuRI GGCC 3 cut(s) 72, 117, 387
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 2 cut(s) 97, 1021
BtgZI GCGATG 1 cut(s) 712
BtsIMutI CAGTG 2 cut(s) 1018, 1132
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 733, 1044
Cfr13I GGNCC 2 cut(s) 239, 737
ClaI ATCGAT 1 cut(s) 714
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 4 cut(s) 89, 574, 989, 1181
CviQI GTAC 4 cut(s) 89, 574, 989, 1181
DdeI CTNAG 4 cut(s) 235, 1032, 1114, 1199
DpnI GATC 3 cut(s) 370, 783, 942
DpnII GATC 3 cut(s) 368, 781, 940
EaeI YGGCCR 3 cut(s) 70, 115, 385
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 3 cut(s) 97, 388, 1021
Eco24I GRGCYC 1 cut(s) 1205
Eco32I GATATC 2 cut(s) 718, 891
Eco47I GGWCC 2 cut(s) 239, 737
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 3 cut(s) 153, 563, 615
Eco91I GGTNACC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 1203
EcoO109I RGGNCCY 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 196, 739
EcoRV GATATC 2 cut(s) 718, 891
EcoT14I CCWWGG 3 cut(s) 97, 388, 1021
EcoT22I ATGCAT 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 3 cut(s) 97, 388, 1021
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 4 cut(s) 190, 588, 1175, 1177
FbaI TGATCA 1 cut(s) 940
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 1063, 1150
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 2 cut(s) 1063, 1150
FspBI CTAG 2 cut(s) 167, 515
GluI GCNGC 2 cut(s) 1063, 1150
GsaI CCCAGC 1 cut(s) 924
HaeIII GGCC 3 cut(s) 72, 117, 387
HincII GTYRAC 2 cut(s) 333, 694
HindII GTYRAC 2 cut(s) 333, 694
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 1 cut(s) 128
HpaI GTTAAC 2 cut(s) 333, 694
HphI GGTGA 6 cut(s) 349, 358, 686, 1006, 1102, 1147
Hpy166II GTNNAC 5 cut(s) 322, 333, 506, 694, 1084
Hpy188I TCNGA 3 cut(s) 133, 343, 582
Hpy188III TCNNGA 7 cut(s) 167, 276, 299, 372, 721, 873, 956
Hpy8I GTNNAC 5 cut(s) 322, 333, 506, 694, 1084
HpyAV CCTTC 2 cut(s) 587, 879
HpyCH4III ACNGT 5 cut(s) 361, 524, 793, 953, 1180
HpyCH4IV ACGT 2 cut(s) 264, 472
HpyCH4V TGCA 6 cut(s) 305, 448, 555, 819, 1062, 1149
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 252
HpyF3I CTNAG 4 cut(s) 235, 1032, 1114, 1199
HpySE526I ACGT 2 cut(s) 264, 472
Ksp22I TGATCA 1 cut(s) 940
KspAI GTTAAC 2 cut(s) 333, 694
Kzo9I GATC 3 cut(s) 368, 781, 940
LmnI GCTCC 2 cut(s) 260, 842
Lsp1109I GCAGC 2 cut(s) 1074, 1161
LweI GCATC 2 cut(s) 292, 473
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 2 cut(s) 167, 515
MaeII ACGT 2 cut(s) 264, 472
MaeIII GTNAC 3 cut(s) 27, 346, 1003
MalI GATC 3 cut(s) 370, 783, 942
MboI GATC 3 cut(s) 368, 781, 940
MboII GAAGA 6 cut(s) 206, 209, 217, 485, 608, 911
MhlI GDGCHC 3 cut(s) 592, 927, 1205
MlsI TGGCCA 2 cut(s) 72, 117
MluNI TGGCCA 2 cut(s) 72, 117
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 7 cut(s) 169, 337, 365, 1123, 1123, 1126, 1131
Mox20I TGGCCA 2 cut(s) 72, 117
Mph1103I ATGCAT 1 cut(s) 307
MscI TGGCCA 2 cut(s) 72, 117
MseI TTAA 6 cut(s) 332, 423, 500, 693, 852, 1074
MslI CAYNNNNRTG 3 cut(s) 123, 629, 1091
Msp20I TGGCCA 2 cut(s) 72, 117
MspR9I CCNGG 2 cut(s) 198, 741
MvaI CCWGG 2 cut(s) 198, 741
MwoI GCNNNNNNNGC 2 cut(s) 155, 252
NcoI CCATGG 2 cut(s) 97, 1021
NdeII GATC 3 cut(s) 368, 781, 940
NlaIV GGNNCC 5 cut(s) 291, 394, 591, 844, 926
NmuCI GTSAC 2 cut(s) 346, 1003
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 1 cut(s) 809
PciI ACATGT 1 cut(s) 805
PfeI GAWTC 1 cut(s) 128
PflFI GACNNNGTC 2 cut(s) 741, 982
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 2 cut(s) 1064, 1151
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 805
PsiI TTATAA 2 cut(s) 84, 441
Psp124BI GAGCTC 1 cut(s) 1205
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 196, 739
PspEI GGTNACC 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 2 cut(s) 196, 739
PspN4I GGNNCC 5 cut(s) 291, 394, 591, 844, 926
PspPI GGNCC 2 cut(s) 239, 737
PspPPI RGGWCCY 1 cut(s) 239
PstI CTGCAG 1 cut(s) 1064
PsyI GACNNNGTC 2 cut(s) 741, 982
RsaI GTAC 4 cut(s) 90, 575, 990, 1182
RsaNI GTAC 4 cut(s) 89, 574, 989, 1181
RseI CAYNNNNRTG 3 cut(s) 123, 629, 1091
SacI GAGCTC 1 cut(s) 1205
SaqAI TTAA 6 cut(s) 332, 423, 500, 693, 852, 1074
SatI GCNGC 2 cut(s) 1063, 1150
Sau3AI GATC 3 cut(s) 368, 781, 940
Sau96I GGNCC 2 cut(s) 239, 737
ScrFI CCNGG 2 cut(s) 198, 741
SduI GDGCHC 3 cut(s) 592, 927, 1205
SexAI ACCWGGT 1 cut(s) 739
SfaNI GCATC 2 cut(s) 292, 473
SfcI CTRYAG 2 cut(s) 478, 1060
SinI GGWCC 2 cut(s) 239, 737
SmiMI CAYNNNNRTG 3 cut(s) 123, 629, 1091
SsiI CCGC 2 cut(s) 104, 735
SspI AATATT 1 cut(s) 664
SspMI CTAG 2 cut(s) 167, 515
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 2 cut(s) 196, 739
StyI CCWWGG 3 cut(s) 97, 388, 1021
TaaI ACNGT 5 cut(s) 361, 524, 793, 953, 1180
TaiI ACGT 2 cut(s) 267, 475
TaqI TCGA 3 cut(s) 93, 714, 977
TatI WGTACW 1 cut(s) 88
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 6 cut(s) 332, 423, 500, 693, 852, 1074
Tru9I TTAA 6 cut(s) 332, 423, 500, 693, 852, 1074
TscAI CASTG 2 cut(s) 1018, 1132
TseFI GTSAC 2 cut(s) 346, 1003
TseI GCWGC 2 cut(s) 1062, 1149
Tsp45I GTSAC 2 cut(s) 346, 1003
TspDTI ATGAA 3 cut(s) 697, 851, 1109
TspGWI ACGGA 1 cut(s) 976
TspRI CASTG 2 cut(s) 1018, 1132
Tth111I GACNNNGTC 2 cut(s) 741, 982
Van91I CCANNNNNTGG 1 cut(s) 746
VpaK11BI GGWCC 2 cut(s) 239, 737
XapI RAATTY 1 cut(s) 973
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 167, 515
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.