pycom15g06200

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000579
Physical Location & Seq
Reverse (-)
3815386 .. 3817237
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06200.1

Sequence Viewer

Length: 1761 bp
ATGTCGATCATCAACACACTCGTTCGCATTTTAGTTTTCTTCCTCGCTTCGTCATTGTTTATTGGCATCGGACAAGGCCAAAAAATATTAGACGTAACCAAATACGGTGCTGTGGGAGATGGACGGGCAGATGACTCCCAAGCTTTTTTTAAAGCGTGGAAAGATTTATGTCAAGGAAATAATGACGCTACGGATGACATACCGATACTTATTGTGCCGGAGGGGAAAACGTACTTATTGCAACGTATAAAGTTTGAAGGTCCATGCAATTCGAAGAGCGTTCACGTTCAGGTTCTAGGGAATTTGTTGGCTCCTATGCGTGGTGCATGGAAAGAATGCGATTCGGGGGATTGGCTAAGTTTTTCAAACATTGAAAACCTAATGCTCGACGGCTCAGGAAAAATCGACGGCCAAGGGGCTGCGTGGTGGAGCACCAGCCAGTATGATGCGAGATCACGCAAACTCTTGGCAAATAACAACTGCAAACCAAATAAGGCATTGCAGTTCCACAACTGTCAAAACCTTAAACTCAGGGGACTGACTTTTCTGAACAGCCCGCAAGCGCATATTGGTATAAATAGTTGTGACAATGCAGATGTCTCAGATTTGAACATACATGCGCCGGAAGACAGTCCAAACACTGATGGTATTGACATCTCTTCGTCAACCCATGTCACCATTCGCAACTCGTTTATCGGAACAGGCGATGATTGTATCGCCATTGGCGACGGGTGTTCCTTTCTCTACATCACCAACATTGCGTGCGGACCAGGCCATGGAATTAGCGTTGGAAGCTTAGGAGAAGATGGAGCTAGTAATAAAGTGGACAACATATACGTGAAAGATTGTAGTTTTACTGGAACTACAAACGGAGCGAGAATAAAAACGTGGCAGGGCGGCTCCGGGTATGCGAGGAACATCACGTTTGAAAGGATCAAACTTGACAGAGCCAAATACCCTATCATTATTGACCAGTATTATTGCAATGGGGAACACAATTGCAAAAGTCAGTCGTCGGCCGTGGTGGTGGCGGATGTGACGTACAGCGATTTTGAAGGCACTTCTGCGACGGATGAGGCGATTAAACTCAACTGCGACAAAGTGTCGGGTGGCTGTCATAATATTTTGATGGATAGAATTAATATAGTGTCAGCGGTCGGGGATATGAAGATTTTTTCGTCTTGTAACAATGCCGACGGAACTTATGTCGGCACTACGGTGCCCGACGTGGCTTGTCTAAAGAGTTCAACAACGGAGCCTGCGACCCCAACAACACCATCTTCACCAAAAACTACCGCTCCGCCGGTACAAATCCTACCATTGCCATCGCCACCACCTATACCGGTAATGCCGCTGCCGTCGCCGTCACCTGTACCGGTAATGCCACTGCCGTCGCCGTCACCTGTACCGGTAATGCCACTGCCATCGCCGTCACCTGTACCAATCATTCCTCCGCCAGCGCGACCACCGGTATCAGTCATACCACCGCCATCGCTGCCATCAATCACCCCACCACCAGAAGATATCATGCCACCGCCAGTGCCAATCACACCACCACCGCAACCAATCACGCCACCACAGTCAACTGCACCACCTGTGCCACAGGGACTAGACATCACAGGAGGGGCAGGGGAGGCTGGCGAGCTAGATCCTTCGTACTTCCCAAGTGGAGCTCCTGGCAGTGCTAATCTACCTATAAGCTACTACCTATTTTGTCCACTGATGGTGGTCCTTGCTGCAACAACTATAATTTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

587

Amino Acids

62.04

Weight (kDa)

5.01

Isoelectric Point (pI)

59.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 399 2.9e-88 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1219
AccB7I CCANNNNNTGG 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 1298
AccII CGCG 1 cut(s) 1462
AclWI GGATC 2 cut(s) 941, 1643
AcoI YGGCCR 2 cut(s) 409, 1017
AcsI RAATTY 1 cut(s) 301
AfaI GTAC 7 cut(s) 233, 1043, 1308, 1374, 1407, 1440, 1658
AfiI CCNNNNNNNGG 2 cut(s) 320, 776
AgeI ACCGGT 4 cut(s) 1342, 1375, 1408, 1468
AgsI TTSAA 7 cut(s) 257, 366, 374, 610, 929, 1055, 1248
AhdI GACNNNNNGTC 1 cut(s) 1102
AjiI CACGTC 1 cut(s) 1228
AjnI CCWGG 2 cut(s) 769, 1675
AleI CACNNNNGTG 1 cut(s) 1217
AluBI AGCT 6 cut(s) 143, 795, 812, 1645, 1673, 1701
AluI AGCT 6 cut(s) 143, 795, 812, 1645, 1673, 1701
Alw21I GWGCWC 2 cut(s) 434, 1675
Alw26I GTCTC 1 cut(s) 604
AlwI GGATC 2 cut(s) 941, 1643
AoxI GGCC 4 cut(s) 76, 409, 772, 1017
ApeKI GCWGC 4 cut(s) 419, 1354, 1495, 1736
ApoI RAATTY 1 cut(s) 301
AseI ATTAAT 1 cut(s) 1140
AsiGI ACCGGT 4 cut(s) 1342, 1375, 1408, 1468
AspLEI GCGC 3 cut(s) 565, 622, 1462
AspS9I GGNCC 3 cut(s) 260, 767, 1729
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 7 cut(s) 667, 742, 1275, 1359, 1392, 1425, 1498
AsuII TTCGAA 1 cut(s) 272
AvaII GGWCC 3 cut(s) 260, 767, 1729
BaeGI GKGCMC 1 cut(s) 1224
BanI GGYRCC 1 cut(s) 1219
BanII GRGCYC 1 cut(s) 1675
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 2 cut(s) 434, 1675
BbvI GCAGC 4 cut(s) 406, 1341, 1482, 1723
BceAI ACGGC 8 cut(s) 406, 424, 1004, 1342, 1348, 1375, 1381, 1414
BciT130I CCWGG 2 cut(s) 771, 1677
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 1 cut(s) 604
BfaI CTAG 4 cut(s) 296, 813, 1610, 1646
BisI GCNGC 6 cut(s) 420, 898, 1352, 1355, 1496, 1737
BlsI GCNGC 6 cut(s) 421, 899, 1353, 1356, 1497, 1738
Bme1390I CCNGG 3 cut(s) 771, 904, 1677
Bme18I GGWCC 3 cut(s) 260, 767, 1729
BmeRI GACNNNNNGTC 1 cut(s) 1102
BmgBI CACGTC 1 cut(s) 1228
BmgT120I GGNCC 3 cut(s) 260, 767, 1729
BmiI GGNNCC 4 cut(s) 312, 901, 1221, 1257
BmrFI CCNGG 3 cut(s) 771, 904, 1677
BmsI GCATC 2 cut(s) 75, 436
BpiI GAAGAC 1 cut(s) 633
Bpu10I CCTNAGC 2 cut(s) 394, 796
Bpu14I TTCGAA 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 904
BsaAI YACGTR 1 cut(s) 838
BsaJI CCNNGG 3 cut(s) 412, 775, 1020
BsaWI WCCGGW 4 cut(s) 1342, 1375, 1408, 1468
BsaXI ACNNNNNCTCC 2 cut(s) 1282, 1312
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 776
Bse118I RCCGGY 5 cut(s) 1303, 1342, 1375, 1408, 1468
Bse1I ACTGG 4 cut(s) 439, 862, 973, 1538
Bse3DI GCAATG 4 cut(s) 497, 756, 991, 1319
BseBI CCWGG 2 cut(s) 771, 1677
BseDI CCNNGG 3 cut(s) 412, 775, 1020
BseGI GGATG 3 cut(s) 199, 1039, 1078
BseLI CCNNNNNNNGG 2 cut(s) 320, 776
BseMI GCAATG 4 cut(s) 497, 756, 991, 1319
BseMII CTCAG 3 cut(s) 408, 544, 615
BseNI ACTGG 4 cut(s) 439, 862, 973, 1538
BseSI GKGCMC 1 cut(s) 1224
BseX3I CGGCCG 1 cut(s) 1017
BseXI GCAGC 4 cut(s) 406, 1341, 1482, 1723
BsgI GTGCAG 1 cut(s) 1572
Bsh1236I CGCG 1 cut(s) 1462
Bsh1285I CGRYCG 2 cut(s) 1020, 1158
BshFI GGCC 4 cut(s) 78, 411, 774, 1019
BshNI GGYRCC 1 cut(s) 1219
BshTI ACCGGT 4 cut(s) 1342, 1375, 1408, 1468
BsiEI CGRYCG 2 cut(s) 1020, 1158
BsiHKAI GWGCWC 2 cut(s) 434, 1675
BsiSI CCGG 8 cut(s) 218, 623, 903, 1304, 1343, 1376, 1409, 1469
BslFI GGGAC 2 cut(s) 549, 1620
BslI CCNNNNNNNGG 2 cut(s) 320, 776
BsmAI GTCTC 1 cut(s) 604
BsmFI GGGAC 2 cut(s) 549, 1620
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 4 cut(s) 78, 411, 774, 1019
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 434, 1224, 1675
Bsp143I GATC 4 cut(s) 6, 452, 933, 1648
Bsp19I CCATGG 1 cut(s) 775
BspANI GGCC 4 cut(s) 78, 411, 774, 1019
BspCNI CTCAG 3 cut(s) 407, 543, 614
BspFNI CGCG 1 cut(s) 1462
BspLI GGNNCC 4 cut(s) 312, 901, 1221, 1257
BspPI GGATC 2 cut(s) 941, 1643
BspQI GCTCTTC 1 cut(s) 269
BspT104I TTCGAA 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 1219
BsrBI CCGCTC 1 cut(s) 1298
BsrDI GCAATG 4 cut(s) 497, 756, 991, 1319
BsrFI RCCGGY 5 cut(s) 1303, 1342, 1375, 1408, 1468
BsrI ACTGG 4 cut(s) 439, 862, 973, 1538
BssAI RCCGGY 5 cut(s) 1303, 1342, 1375, 1408, 1468
BssECI CCNNGG 3 cut(s) 412, 775, 1020
BssMI GATC 4 cut(s) 6, 452, 933, 1648
BssT1I CCWWGG 2 cut(s) 412, 775
Bst2UI CCWGG 2 cut(s) 771, 1677
Bst4CI ACNGT 5 cut(s) 107, 515, 632, 1219, 1581
Bst6I CTCTTC 2 cut(s) 269, 664
BstBAI YACGTR 1 cut(s) 838
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 7 cut(s) 557, 561, 763, 1260, 1458, 1639, 1643
BstDEI CTNAG 5 cut(s) 356, 394, 530, 601, 796
BstDSI CCRYGG 2 cut(s) 775, 1020
BstF5I GGATG 3 cut(s) 199, 1039, 1078
BstFNI CGCG 1 cut(s) 1462
BstHHI GCGC 3 cut(s) 565, 622, 1462
BstKTI GATC 4 cut(s) 9, 455, 936, 1651
BstMAI GTCTC 1 cut(s) 604
BstMBI GATC 4 cut(s) 6, 452, 933, 1648
BstMCI CGRYCG 2 cut(s) 1020, 1158
BstMWI GCNNNNNNNGC 5 cut(s) 771, 792, 1360, 1495, 1634
BstNI CCWGG 2 cut(s) 771, 1677
BstNSI RCATGY 1 cut(s) 620
BstSCI CCNGG 3 cut(s) 769, 902, 1675
BstSLI GKGCMC 1 cut(s) 1224
BstUI CGCG 1 cut(s) 1462
BstV1I GCAGC 4 cut(s) 406, 1341, 1482, 1723
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 1648
BstYI RGATCY 1 cut(s) 1648
BstZI CGGCCG 1 cut(s) 1017
BsuRI GGCC 4 cut(s) 78, 411, 774, 1019
BtgI CCRYGG 2 cut(s) 775, 1020
BtgZI GCGATG 4 cut(s) 720, 1311, 1410, 1476
BtrI CACGTC 1 cut(s) 1228
BtsCI GGATG 3 cut(s) 199, 1039, 1078
BtsI GCAGTG 3 cut(s) 1385, 1418, 1687
BtsIMutI CAGTG 6 cut(s) 639, 1385, 1418, 1545, 1687, 1718
Cac8I GCNNGC 7 cut(s) 557, 561, 763, 1260, 1458, 1639, 1643
CfoI GCGC 3 cut(s) 565, 622, 1462
Cfr10I RCCGGY 5 cut(s) 1303, 1342, 1375, 1408, 1468
Cfr13I GGNCC 3 cut(s) 260, 767, 1729
CseI GACGC 1 cut(s) 194
Csp6I GTAC 7 cut(s) 232, 1042, 1307, 1373, 1406, 1439, 1657
CspAI ACCGGT 4 cut(s) 1342, 1375, 1408, 1468
CviAII CATG 6 cut(s) 264, 327, 617, 671, 776, 1528
CviQI GTAC 7 cut(s) 232, 1042, 1307, 1373, 1406, 1439, 1657
DdeI CTNAG 5 cut(s) 356, 394, 530, 601, 796
DpnI GATC 4 cut(s) 8, 454, 935, 1650
DpnII GATC 4 cut(s) 6, 452, 933, 1648
DraI TTTAAA 1 cut(s) 151
DriI GACNNNNNGTC 1 cut(s) 1102
EaeI YGGCCR 2 cut(s) 409, 1017
EagI CGGCCG 1 cut(s) 1017
Eam1104I CTCTTC 2 cut(s) 269, 664
Eam1105I GACNNNNNGTC 1 cut(s) 1102
EarI CTCTTC 2 cut(s) 269, 664
EciI GGCGGA 3 cut(s) 1046, 1290, 1443
Ecl136II GAGCTC 1 cut(s) 1673
EclXI CGGCCG 1 cut(s) 1017
Eco130I CCWWGG 2 cut(s) 412, 775
Eco24I GRGCYC 1 cut(s) 1675
Eco32I GATATC 1 cut(s) 1525
Eco47I GGWCC 3 cut(s) 260, 767, 1729
Eco52I CGGCCG 1 cut(s) 1017
Eco53kI GAGCTC 1 cut(s) 1673
EcoICRI GAGCTC 1 cut(s) 1673
EcoRII CCWGG 2 cut(s) 769, 1675
EcoRV GATATC 1 cut(s) 1525
EcoT14I CCWWGG 2 cut(s) 412, 775
EcoT38I GRGCYC 1 cut(s) 1675
ErhI CCWWGG 2 cut(s) 412, 775
FaeI CATG 6 cut(s) 267, 330, 620, 674, 779, 1531
FaqI GGGAC 2 cut(s) 549, 1620
FatI CATG 6 cut(s) 263, 326, 616, 670, 775, 1527
FauI CCCGC 1 cut(s) 564
Fnu4HI GCNGC 6 cut(s) 420, 898, 1352, 1355, 1496, 1737
FokI GGATG 3 cut(s) 206, 1046, 1085
FriOI GRGCYC 1 cut(s) 1675
Fsp4HI GCNGC 6 cut(s) 420, 898, 1352, 1355, 1496, 1737
FspBI CTAG 4 cut(s) 296, 813, 1610, 1646
GlaI GCGC 3 cut(s) 564, 621, 1461
GluI GCNGC 6 cut(s) 420, 898, 1352, 1355, 1496, 1737
HaeIII GGCC 4 cut(s) 78, 411, 774, 1019
HapII CCGG 8 cut(s) 218, 623, 903, 1304, 1343, 1376, 1409, 1469
HgaI GACGC 1 cut(s) 194
HhaI GCGC 3 cut(s) 565, 622, 1462
Hin1II CATG 6 cut(s) 267, 330, 620, 674, 779, 1531
Hin6I GCGC 3 cut(s) 563, 620, 1460
HinP1I GCGC 3 cut(s) 563, 620, 1460
HincII GTYRAC 2 cut(s) 666, 1584
HindII GTYRAC 2 cut(s) 666, 1584
HindIII AAGCTT 2 cut(s) 141, 793
HinfI GANTC 2 cut(s) 134, 341
HpaII CCGG 8 cut(s) 218, 623, 903, 1304, 1343, 1376, 1409, 1469
HphI GGTGA 7 cut(s) 667, 742, 1275, 1359, 1392, 1425, 1498
Hpy166II GTNNAC 5 cut(s) 283, 666, 826, 1584, 1718
Hpy188I TCNGA 4 cut(s) 71, 549, 604, 698
Hpy188III TCNNGA 1 cut(s) 396
Hpy8I GTNNAC 5 cut(s) 283, 666, 826, 1584, 1718
Hpy99I CGWCG 9 cut(s) 392, 410, 731, 1018, 1072, 1199, 1229, 1363, 1396
HpyAV CCTTC 3 cut(s) 251, 1049, 1662
HpyCH4III ACNGT 5 cut(s) 107, 515, 632, 1219, 1581
HpyCH4IV ACGT 9 cut(s) 93, 230, 244, 285, 837, 887, 923, 1040, 1227
HpyF10VI GCNNNNNNNGC 5 cut(s) 771, 792, 1360, 1495, 1634
HpyF3I CTNAG 5 cut(s) 356, 394, 530, 601, 796
HpySE526I ACGT 9 cut(s) 93, 230, 244, 285, 837, 887, 923, 1040, 1227
Hsp92II CATG 6 cut(s) 267, 330, 620, 674, 779, 1531
HspAI GCGC 3 cut(s) 563, 620, 1460
Kzo9I GATC 4 cut(s) 6, 452, 933, 1648
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 9 cut(s) 316, 429, 809, 872, 905, 1255, 1303, 1670, 1678
Lsp1109I GCAGC 4 cut(s) 406, 1341, 1482, 1723
LweI GCATC 2 cut(s) 75, 436
MaeI CTAG 4 cut(s) 296, 813, 1610, 1646
MaeII ACGT 9 cut(s) 93, 230, 244, 285, 837, 887, 923, 1040, 1227
MaeIII GTNAC 8 cut(s) 94, 584, 673, 1036, 1184, 1365, 1398, 1431
MalI GATC 4 cut(s) 8, 454, 935, 1650
MbiI CCGCTC 1 cut(s) 1298
MboI GATC 4 cut(s) 6, 452, 933, 1648
MboII GAAGA 8 cut(s) 31, 286, 638, 651, 815, 1180, 1272, 1532
MfeI CAATTG 1 cut(s) 997
MflI RGATCY 1 cut(s) 1648
MhlI GDGCHC 3 cut(s) 434, 1224, 1675
MluCI AATT 7 cut(s) 268, 301, 780, 997, 1137, 1749, 1756
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 769
MnlI CCTC 7 cut(s) 53, 214, 906, 1069, 1461, 1616, 1627
MseI TTAA 6 cut(s) 150, 525, 1083, 1140, 1755, 1759
MslI CAYNNNNRTG 2 cut(s) 836, 1217
MspA1I CMGCKG 2 cut(s) 1154, 1354
MspI CCGG 8 cut(s) 218, 623, 903, 1304, 1343, 1376, 1409, 1469
MspR9I CCNGG 3 cut(s) 771, 904, 1677
MunI CAATTG 1 cut(s) 997
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 2 cut(s) 771, 1677
MvnI CGCG 1 cut(s) 1462
MwoI GCNNNNNNNGC 5 cut(s) 771, 792, 1360, 1495, 1634
NciI CCSGG 1 cut(s) 904
NcoI CCATGG 1 cut(s) 775
NdeII GATC 4 cut(s) 6, 452, 933, 1648
NlaIII CATG 6 cut(s) 267, 330, 620, 674, 779, 1531
NlaIV GGNNCC 4 cut(s) 312, 901, 1221, 1257
NmuCI GTSAC 6 cut(s) 584, 673, 1036, 1365, 1398, 1431
NspI RCATGY 1 cut(s) 620
NspV TTCGAA 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 1217
PacI TTAATTAA 1 cut(s) 1759
PciSI GCTCTTC 1 cut(s) 269
PcsI WCGNNNNNNNCGW 4 cut(s) 702, 723, 1076, 1259
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 776
PinAI ACCGGT 4 cut(s) 1342, 1375, 1408, 1468
PkrI GCNGC 6 cut(s) 421, 899, 1353, 1356, 1497, 1738
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 838
PshBI ATTAAT 1 cut(s) 1140
Psp124BI GAGCTC 1 cut(s) 1675
Psp6I CCWGG 2 cut(s) 769, 1675
PspGI CCWGG 2 cut(s) 769, 1675
PspN4I GGNNCC 4 cut(s) 312, 901, 1221, 1257
PspPI GGNCC 3 cut(s) 260, 767, 1729
PsuI RGATCY 1 cut(s) 1648
RsaI GTAC 7 cut(s) 233, 1043, 1308, 1374, 1407, 1440, 1658
RsaNI GTAC 7 cut(s) 232, 1042, 1307, 1373, 1406, 1439, 1657
RseI CAYNNNNRTG 2 cut(s) 836, 1217
SacI GAGCTC 1 cut(s) 1675
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 6 cut(s) 150, 525, 1083, 1140, 1755, 1759
SatI GCNGC 6 cut(s) 420, 898, 1352, 1355, 1496, 1737
Sau3AI GATC 4 cut(s) 6, 452, 933, 1648
Sau96I GGNCC 3 cut(s) 260, 767, 1729
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 771, 904, 1677
SduI GDGCHC 3 cut(s) 434, 1224, 1675
SfaNI GCATC 2 cut(s) 75, 436
SfuI TTCGAA 1 cut(s) 272
SinI GGWCC 3 cut(s) 260, 767, 1729
SmiMI CAYNNNNRTG 2 cut(s) 836, 1217
Sse9I AATT 7 cut(s) 268, 301, 780, 997, 1137, 1749, 1756
SspI AATATT 2 cut(s) 87, 1123
SspMI CTAG 4 cut(s) 296, 813, 1610, 1646
SstI GAGCTC 1 cut(s) 1675
StyD4I CCNGG 3 cut(s) 769, 902, 1675
StyI CCWWGG 2 cut(s) 412, 775
TaaI ACNGT 5 cut(s) 107, 515, 632, 1219, 1581
TaiI ACGT 9 cut(s) 96, 233, 247, 288, 840, 890, 926, 1043, 1230
TaqI TCGA 4 cut(s) 5, 272, 387, 405
TasI AATT 7 cut(s) 268, 301, 780, 997, 1137, 1749, 1756
TauI GCSGC 2 cut(s) 900, 1354
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 6 cut(s) 150, 525, 1083, 1140, 1755, 1759
Tru9I TTAA 6 cut(s) 150, 525, 1083, 1140, 1755, 1759
TscAI CASTG 6 cut(s) 646, 1392, 1425, 1545, 1687, 1725
TseFI GTSAC 6 cut(s) 584, 673, 1036, 1365, 1398, 1431
TseI GCWGC 4 cut(s) 419, 1354, 1495, 1736
Tsp45I GTSAC 6 cut(s) 584, 673, 1036, 1365, 1398, 1431
TspDTI ATGAA 1 cut(s) 1181
TspGWI ACGGA 5 cut(s) 206, 885, 1085, 1212, 1268
TspRI CASTG 6 cut(s) 646, 1392, 1425, 1545, 1687, 1725
Van91I CCANNNNNTGG 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 260, 767, 1729
VspI ATTAAT 1 cut(s) 1140
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 620
XspI CTAG 4 cut(s) 296, 813, 1610, 1646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.