MD15G1124300.v1.1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000579
Physical Location & Seq
Reverse (-)
9028868 .. 9035904
7037 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1124300.v1.1.491

Sequence Viewer

Length: 2307 bp
ATGCAGGAACTACTGCGCACCAGTTTGATGCTTTATATTGCTTTAACGAGTTTTGTGTTTGGCGCTGTGGATGAAACCCGAAGTAATATTTTTAACGTGCTTGACTATGGCGCCTTTGCAGATGGCCAGCACGATGATTCCGAAGCCTTTTTAAAAGCATGGAAGGAGTTGTGTGAAGCTGAAGGAAGTTATTATGGTCTGCCAATGCTTGAAATACCTTGCGGAAAGACATTTAAACTGAAACCCGTGCATTTCCAGGGCCCCTGCAAGTCGAGATCAGTTCAAATTAAGTTTTTTGGAACTATCATTGCTCCTGCGTATATAAGTGGATGGACTGGTTGTGTTGGAAATAGCTGGCTACTATTTTCAAACGTGGACAACCTCATGATCAATGGATCGGGAAAGATCGACGGCCAGGGTGAAAATTGGTGGCATAAACCAAAGGAACACCAAAAACACAAAGTACACATTTCTCATTCTATATGCATATTAACCTTATCCAAATTACATCATGACACAGGTAACCAAACTTTAATTTATGTCCAGGAAATAATCAGATACCAATCTCTCGATTTTCAGGGTGAAGGAAACTGCTATAGTCCTGCAGCTTTACGCCTCAGCAACTGCTCTAATCTTCATCTTAGGGGACTTACTCATGTTAATAGCCCAAGAAGCCATATTTATATCAGTAACAGTGAAAATGTAACTCTTACTAATCTCAATATAACTGCACCTGAATCAAGCCCTAACACAGATGGAATTCATCTCTCAAGCTCAAAAAATGTGAGCATCCATGAAAGCACCATTGCAACTGGGGATGACTGCATTGCTATCAAGCGAAATTGTTCCAAGATCAATATTAAAGGGATTATGTGTGGCCCAGGGCATGGTATAAGCGTGGGAAGCCTTGGAGCACAAGGAGCGTATGAAACAGTTGAAGAAGTGTTCGTGGGAAATTGTACCTTCACAGAGTCCATGTATGGAGCAAGGATCAAGACCTGGCAGGGAGGTTCAGGGTACGCCAGGAACATCCATTTCCAACACATCCATCTTAGGGAAGCTAAAAATCCGATCATCATTGACCAATTTTACTGCAATGGTCGACACGATTGCAAAAATTCGACGGAGGCAGTGTCAGTGAGCAATGTTACATATTTTGGTGTGAGGGGAACTAGTGCTAGGAAGGATGCAATCACATTGAAGTGTGCAAGTACAAATTGCACCAACATTTCAATGAACAAGATTAACATTTCATCATGGGACTATGGAAGTGAGGTTTCTGCTTATTGTCTGAATGCAAAAGGAACATCAAGCTGCACCACACCTACTGTGCCTTGCCTGAACGGTGTTTGCGAGGGTGGAGGTGACTGTGGAAGTGAAGATTTTGACCTACATTTAGTCAGCTTTCCGTTTTTATTCATTGCTACATCTAGTTTTGGCATTGGATATGGGCAAAATGGTACTTATAATGTGTTTGATTTTGGTGCATCTGGAGATGGCGTCGCAGATGATTCACAAACATGGCAAAGAGCATGTGGAACTCCAGGATCTTCACAAGCCCTAAGCATCCCACAAGGGAAAACCTACTTACTCAACCCTCTCAGATTTTTGGGTCCTTGCAAGTCCAACAATGTTCAAATTCAGGTTGACGGGAATATTGTGGGTCCTTCCGATCCTAATGTATGGACAACGGCGTGCGAATCTGGATGCTGGTTATGCTTCGAAGACGTGAAGGGTCTGATCATCAAGGGAAGCGGACATGTAAATGGGAAGGGTGCAATTTGGTGGAACCAAGCTCTACACTTTCACAAATGTGACAACCTTCAACTTAGTGGATTTACTTCTCAAGACAGCCCAAGAAATCATATCATGATCCATCAATGCACTGGTGTACATATATCAAGTGTTCATCTAAATGCACCTGCAAATAGCCCCAACACTGATGGCATTGACATCAGTTTGTCAAGTCAAATAGATATACATGACTCTTCCGTTGGAACCAGTGATGACTGCATTGCAATTAAGGGTGGCTCTTCTTTTGTAAACATAACAAATGTGGCATGTGGACCAGGTCACGGTATTAGCTTAGGACAAGGTGAAGCTGATGATAGAGTTGAACAAGTGCATGTTCAACATTGTACCTTCACCAATACTCAAAATGGTGCAAGAATCAAGACTTGGAAGGGAGGATATGGGTATGCTAAGTCGATTTTCTTTAGTCAAATCACACTTGTTCTAGCTCAAAATCCTATTATTATTGACCAGCATTACATCGATACAACTAAAAGCAAAGATTCCGTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

769

Amino Acids

83.37

Weight (kDa)

6.28

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 69 - 158 3.7e-10 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 199 - 439 1.1e-69 Glycosyl hydrolases family 28
Pect-lyase_RHGA_epim PF12708 490 - 725 1.2e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 518 - 763 1.4e-56 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1467
AarI CACCTGC 1 cut(s) 1930
Acc16I TGCGCA 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 1930
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 887
AccI GTMKAC 1 cut(s) 1102
AciI CCGC 2 cut(s) 222, 1755
AclWI GGATC 5 cut(s) 403, 998, 1555, 1667, 1867
AcoI YGGCCR 2 cut(s) 124, 412
AcsI RAATTY 3 cut(s) 759, 1117, 1638
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 2 cut(s) 111, 1500
AfaI GTAC 7 cut(s) 465, 961, 1019, 1213, 1462, 1893, 2140
AfiI CCNNNNNNNGG 3 cut(s) 887, 1054, 2075
AflIII ACRYGT 1 cut(s) 1759
AhlI ACTAGT 1 cut(s) 1172
AjiI CACGTC 1 cut(s) 1729
AjnI CCWGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
AjuI GAANNNNNNNTTGG 2 cut(s) 2161, 2193
AleI CACNNNNGTG 1 cut(s) 1812
Alw21I GWGCWC 1 cut(s) 916
AlwI GGATC 5 cut(s) 403, 998, 1555, 1667, 1867
AlwNI CAGNNNCTG 1 cut(s) 624
AoxI GGCC 4 cut(s) 124, 259, 412, 877
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 605, 1314
ApoI RAATTY 3 cut(s) 759, 1117, 1638
Asp700I GAANNNNTTC 1 cut(s) 844
AspLEI GCGC 3 cut(s) 18, 65, 113
AspS9I GGNCC 6 cut(s) 259, 260, 878, 1613, 1664, 2066
AsuHPI GGTGA 5 cut(s) 431, 593, 1376, 2108, 2137
AsuII TTCGAA 1 cut(s) 1722
AvaII GGWCC 3 cut(s) 1613, 1664, 2066
BaeGI GKGCMC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 1731
Bbv12I GWGCWC 1 cut(s) 916
BbvCI CCTCAGC 1 cut(s) 617
BbvI GCAGC 2 cut(s) 617, 1301
BccI CCATC 7 cut(s) 116, 324, 749, 1056, 1490, 1884, 1937
BceAI ACGGC 2 cut(s) 427, 1707
BcgI CGANNNNNNTGC 2 cut(s) 1092, 1126
BciT130I CCWGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
BclI TGATCA 2 cut(s) 387, 1740
BcuI ACTAGT 1 cut(s) 1172
BfaI CTAG 4 cut(s) 1173, 1179, 1431, 2237
BfmI CTRYAG 2 cut(s) 595, 603
BfoI RGCGCY 2 cut(s) 66, 114
BfuAI ACCTGC 1 cut(s) 1930
BisI GCNGC 2 cut(s) 606, 1315
BlsI GCNGC 2 cut(s) 607, 1316
Bme1390I CCNGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
Bme18I GGWCC 3 cut(s) 1613, 1664, 2066
BmgBI CACGTC 1 cut(s) 1729
BmgT120I GGNCC 6 cut(s) 259, 260, 878, 1613, 1664, 2066
BmiI GGNNCC 7 cut(s) 112, 261, 262, 1614, 1665, 1790, 1999
BmrFI CCNGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 6 cut(s) 18, 798, 1177, 1496, 1575, 1697
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 1731
BpmI CTGGAG 2 cut(s) 1512, 1527
Bpu10I CCTNAGC 3 cut(s) 617, 1562, 2086
Bpu14I TTCGAA 1 cut(s) 1722
BpuEI CTTGAG 2 cut(s) 754, 1830
Bsa29I ATCGAT 1 cut(s) 2274
BsaBI GATNNNNATC 1 cut(s) 562
BsaHI GRCGYC 2 cut(s) 111, 1500
BsaJI CCNNGG 5 cut(s) 256, 415, 880, 881, 907
BsaXI ACNNNNNCTCC 4 cut(s) 1118, 1148, 1485, 1515
Bsc4I CCNNNNNNNGG 3 cut(s) 887, 1054, 2075
Bse1I ACTGG 5 cut(s) 21, 340, 817, 1891, 2001
Bse3DI GCAATG 7 cut(s) 306, 804, 825, 1102, 1150, 1419, 2013
Bse8I GATNNNNATC 1 cut(s) 562
BseBI CCWGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
BseCI ATCGAT 1 cut(s) 2274
BseDI CCNNGG 5 cut(s) 256, 415, 880, 881, 907
BseGI GGATG 9 cut(s) 76, 335, 789, 823, 1029, 1044, 1192, 1566, 1712
BseJI GATNNNNATC 1 cut(s) 562
BseLI CCNNNNNNNGG 3 cut(s) 887, 1054, 2075
BseMI GCAATG 7 cut(s) 306, 804, 825, 1102, 1150, 1419, 2013
BseMII CTCAG 2 cut(s) 631, 1615
BseNI ACTGG 5 cut(s) 21, 340, 817, 1891, 2001
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 617, 1301
BsgI GTGCAG 2 cut(s) 714, 1300
BshFI GGCC 4 cut(s) 126, 261, 414, 879
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 2274
BsiHKAI GWGCWC 1 cut(s) 916
BslFI GGGAC 2 cut(s) 660, 1274
BslI CCNNNNNNNGG 3 cut(s) 887, 1054, 2075
BsmFI GGGAC 2 cut(s) 660, 1274
BsmI GAATGC 1 cut(s) 1300
BsnI GGCC 4 cut(s) 126, 261, 414, 879
Bsp119I TTCGAA 1 cut(s) 1722
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 263, 916
Bsp1407I TGTACA 1 cut(s) 1891
BspACI CCGC 2 cut(s) 222, 1755
BspANI GGCC 4 cut(s) 126, 261, 414, 879
BspCNI CTCAG 2 cut(s) 630, 1614
BspDI ATCGAT 1 cut(s) 2274
BspHI TCATGA 3 cut(s) 384, 511, 1869
BspLI GGNNCC 7 cut(s) 112, 261, 262, 1614, 1665, 1790, 1999
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 1 cut(s) 1930
BspPI GGATC 5 cut(s) 403, 998, 1555, 1667, 1867
BspQI GCTCTTC 1 cut(s) 2038
BspT104I TTCGAA 1 cut(s) 1722
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 7 cut(s) 306, 804, 825, 1102, 1150, 1419, 2013
BsrGI TGTACA 1 cut(s) 1891
BsrI ACTGG 5 cut(s) 21, 340, 817, 1891, 2001
BssECI CCNNGG 5 cut(s) 256, 415, 880, 881, 907
BssNI GRCGYC 2 cut(s) 111, 1500
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
Bst4CI ACNGT 6 cut(s) 695, 934, 1330, 1346, 1370, 2078
Bst6I CTCTTC 2 cut(s) 1993, 2038
BstACI GRCGYC 2 cut(s) 111, 1500
BstAUI TGTACA 1 cut(s) 1891
BstBI TTCGAA 1 cut(s) 1722
BstC8I GCNNGC 3 cut(s) 128, 356, 1696
BstDEI CTNAG 8 cut(s) 617, 641, 1052, 1562, 1601, 1829, 2086, 2202
BstEII GGTNACC 1 cut(s) 521
BstF5I GGATG 9 cut(s) 76, 335, 789, 823, 1029, 1044, 1192, 1566, 1712
BstH2I RGCGCY 2 cut(s) 66, 114
BstHHI GCGC 3 cut(s) 18, 65, 113
BstMWI GCNNNNNNNGC 4 cut(s) 672, 903, 920, 1716
BstNI CCWGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
BstNSI RCATGY 4 cut(s) 1536, 1763, 2064, 2129
BstPI GGTNACC 1 cut(s) 521
BstSCI CCNGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
BstSFI CTRYAG 2 cut(s) 595, 603
BstSLI GKGCMC 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 617, 1301
BstV2I GAAGAC 1 cut(s) 1731
BstX2I RGATCY 1 cut(s) 1547
BstYI RGATCY 1 cut(s) 1547
Bsu15I ATCGAT 1 cut(s) 2274
BsuRI GGCC 4 cut(s) 126, 261, 414, 879
BsuTUI ATCGAT 1 cut(s) 2274
BtrI CACGTC 1 cut(s) 1729
BtsCI GGATG 9 cut(s) 76, 335, 789, 823, 1029, 1044, 1192, 1566, 1712
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 6 cut(s) 700, 1137, 1143, 1884, 1938, 2008
BveI ACCTGC 1 cut(s) 1930
Cac8I GCNNGC 3 cut(s) 128, 356, 1696
CaiI CAGNNNCTG 1 cut(s) 624
CciI TCATGA 3 cut(s) 384, 511, 1869
CfoI GCGC 3 cut(s) 18, 65, 113
Cfr13I GGNCC 6 cut(s) 259, 260, 878, 1613, 1664, 2066
ClaI ATCGAT 1 cut(s) 2274
CseI GACGC 1 cut(s) 1489
CsiI ACCWGGT 1 cut(s) 2068
Csp6I GTAC 7 cut(s) 464, 960, 1018, 1212, 1461, 1892, 2139
CviQI GTAC 7 cut(s) 464, 960, 1018, 1212, 1461, 1892, 2139
DdeI CTNAG 8 cut(s) 617, 641, 1052, 1562, 1601, 1829, 2086, 2202
DinI GGCGCC 1 cut(s) 112
DraI TTTAAA 2 cut(s) 153, 235
EaeI YGGCCR 2 cut(s) 124, 412
Eam1104I CTCTTC 2 cut(s) 1993, 2038
EarI CTCTTC 2 cut(s) 1993, 2038
Eco130I CCWWGG 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 263
Eco47I GGWCC 3 cut(s) 1613, 1664, 2066
Eco57I CTGAAG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 521
EcoO109I RGGNCCY 4 cut(s) 259, 260, 1613, 1664
EcoO65I GGTNACC 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 759
EcoRII CCWGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
EcoT14I CCWWGG 1 cut(s) 907
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 263
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 907
FaqI GGGAC 2 cut(s) 660, 1274
FbaI TGATCA 2 cut(s) 387, 1740
FblI GTMKAC 1 cut(s) 1102
Fnu4HI GCNGC 2 cut(s) 606, 1315
FokI GGATG 9 cut(s) 83, 342, 776, 830, 1016, 1031, 1199, 1553, 1719
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 606, 1315
FspBI CTAG 4 cut(s) 1173, 1179, 1431, 2237
FspI TGCGCA 1 cut(s) 17
GlaI GCGC 3 cut(s) 17, 64, 112
GluI GCNGC 2 cut(s) 606, 1315
GsuI CTGGAG 2 cut(s) 1512, 1527
HaeII RGCGCY 2 cut(s) 66, 114
HaeIII GGCC 4 cut(s) 126, 261, 414, 879
HgaI GACGC 1 cut(s) 1489
HhaI GCGC 3 cut(s) 18, 65, 113
Hin1I GRCGYC 2 cut(s) 111, 1500
Hin6I GCGC 3 cut(s) 16, 63, 111
HinP1I GCGC 3 cut(s) 16, 63, 111
HincII GTYRAC 2 cut(s) 1103, 1648
HindII GTYRAC 2 cut(s) 1103, 1648
HinfI GANTC 8 cut(s) 137, 737, 971, 1511, 1700, 1985, 2169, 2294
HphI GGTGA 5 cut(s) 431, 593, 1376, 2108, 2137
Hpy166II GTNNAC 7 cut(s) 376, 466, 1103, 1648, 1892, 2044, 2066
Hpy188I TCNGA 7 cut(s) 142, 557, 1071, 1293, 1604, 1672, 1740
Hpy8I GTNNAC 7 cut(s) 376, 466, 1103, 1648, 1892, 2044, 2066
Hpy99I CGWCG 3 cut(s) 413, 1126, 1505
HpyCH4III ACNGT 6 cut(s) 695, 934, 1330, 1346, 1370, 2078
HpyCH4IV ACGT 3 cut(s) 96, 372, 1728
HpyF10VI GCNNNNNNNGC 4 cut(s) 672, 903, 920, 1716
HpyF3I CTNAG 8 cut(s) 617, 641, 1052, 1562, 1601, 1829, 2086, 2202
HpySE526I ACGT 3 cut(s) 96, 372, 1728
Hsp92I GRCGYC 2 cut(s) 111, 1500
HspAI GCGC 3 cut(s) 16, 63, 111
KasI GGCGCC 1 cut(s) 110
Ksp22I TGATCA 2 cut(s) 387, 1740
LguI GCTCTTC 1 cut(s) 2038
LmnI GCTCC 4 cut(s) 316, 911, 920, 983
Lsp1109I GCAGC 2 cut(s) 617, 1301
LweI GCATC 6 cut(s) 18, 798, 1177, 1496, 1575, 1697
MabI ACCWGGT 1 cut(s) 2068
MaeI CTAG 4 cut(s) 1173, 1179, 1431, 2237
MaeII ACGT 3 cut(s) 96, 372, 1728
MaeIII GTNAC 7 cut(s) 521, 689, 703, 1147, 1364, 1814, 2072
MboII GAAGA 7 cut(s) 626, 950, 1391, 1542, 1736, 1980, 2025
MflI RGATCY 1 cut(s) 1547
MhlI GDGCHC 2 cut(s) 263, 916
MlsI TGGCCA 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 2 cut(s) 980, 1979
MmeI TCCRAC 4 cut(s) 325, 1063, 1650, 1975
Mox20I TGGCCA 1 cut(s) 126
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 844
MscI TGGCCA 1 cut(s) 126
MslI CAYNNNNRTG 6 cut(s) 1201, 1232, 1519, 1764, 1812, 1914
Msp20I TGGCCA 1 cut(s) 126
MspR9I CCNGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
Mva1269I GAATGC 1 cut(s) 1300
MvaI CCWGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
MwoI GCNNNNNNNGC 4 cut(s) 672, 903, 920, 1716
NarI GGCGCC 1 cut(s) 111
NlaIV GGNNCC 7 cut(s) 112, 261, 262, 1614, 1665, 1790, 1999
NmuCI GTSAC 3 cut(s) 1364, 1814, 2072
NsbI TGCGCA 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 4 cut(s) 1536, 1763, 2064, 2129
NspV TTCGAA 1 cut(s) 1722
OliI CACNNNNGTG 1 cut(s) 1812
PagI TCATGA 3 cut(s) 384, 511, 1869
PaqCI CACCTGC 1 cut(s) 1930
PasI CCCWGGG 1 cut(s) 881
PciI ACATGT 1 cut(s) 1759
PciSI GCTCTTC 1 cut(s) 2038
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 1350
PctI GAATGC 1 cut(s) 1300
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 6 cut(s) 137, 737, 1511, 1700, 2169, 2294
PflFI GACNNNGTC 1 cut(s) 2070
PflMI CCANNNNNTGG 1 cut(s) 887
PfoI TCCNGGA 2 cut(s) 543, 1543
PkrI GCNGC 2 cut(s) 607, 1316
PleI GAGTC 2 cut(s) 979, 1979
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 2 cut(s) 979, 1979
PpuMI RGGWCCY 2 cut(s) 1613, 1664
PscI ACATGT 1 cut(s) 1759
PsiI TTATAA 1 cut(s) 1467
Psp5II RGGWCCY 2 cut(s) 1613, 1664
Psp6I CCWGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
PspEI GGTNACC 1 cut(s) 521
PspGI CCWGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
PspN4I GGNNCC 7 cut(s) 112, 261, 262, 1614, 1665, 1790, 1999
PspOMI GGGCCC 1 cut(s) 259
PspPI GGNCC 6 cut(s) 259, 260, 878, 1613, 1664, 2066
PspPPI RGGWCCY 2 cut(s) 1613, 1664
PstI CTGCAG 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 624
PsuI RGATCY 1 cut(s) 1547
PsyI GACNNNGTC 1 cut(s) 2070
RsaI GTAC 7 cut(s) 465, 961, 1019, 1213, 1462, 1893, 2140
RsaNI GTAC 7 cut(s) 464, 960, 1018, 1212, 1461, 1892, 2139
RseI CAYNNNNRTG 6 cut(s) 1201, 1232, 1519, 1764, 1812, 1914
SalI GTCGAC 1 cut(s) 1101
SapI GCTCTTC 1 cut(s) 2038
SatI GCNGC 2 cut(s) 606, 1315
Sau96I GGNCC 6 cut(s) 259, 260, 878, 1613, 1664, 2066
SchI GAGTC 2 cut(s) 980, 1979
ScrFI CCNGG 8 cut(s) 257, 416, 545, 882, 1000, 1024, 1545, 2070
SduI GDGCHC 2 cut(s) 263, 916
SexAI ACCWGGT 1 cut(s) 2068
SfaNI GCATC 6 cut(s) 18, 798, 1177, 1496, 1575, 1697
SfcI CTRYAG 2 cut(s) 595, 603
SfoI GGCGCC 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 1722
SinI GGWCC 3 cut(s) 1613, 1664, 2066
SmiMI CAYNNNNRTG 6 cut(s) 1201, 1232, 1519, 1764, 1812, 1914
SmlI CTYRAG 2 cut(s) 769, 1845
SmoI CTYRAG 2 cut(s) 769, 1845
SpeI ACTAGT 1 cut(s) 1172
SsiI CCGC 2 cut(s) 222, 1755
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 3 cut(s) 88, 859, 1657
SspMI CTAG 4 cut(s) 1173, 1179, 1431, 2237
StyD4I CCNGG 8 cut(s) 255, 414, 543, 880, 998, 1022, 1543, 2068
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 6 cut(s) 695, 934, 1330, 1346, 1370, 2078
TaiI ACGT 3 cut(s) 99, 375, 1731
TaqI TCGA 8 cut(s) 272, 408, 570, 1102, 1121, 1722, 2207, 2274
TatI WGTACW 3 cut(s) 463, 1211, 1891
TfiI GAWTC 6 cut(s) 137, 737, 1511, 1700, 2169, 2294
TscAI CASTG 6 cut(s) 700, 1137, 1143, 1891, 1945, 2008
TseFI GTSAC 3 cut(s) 1364, 1814, 2072
TseI GCWGC 2 cut(s) 605, 1314
Tsp45I GTSAC 3 cut(s) 1364, 1814, 2072
TspDTI ATGAA 9 cut(s) 87, 626, 752, 810, 942, 1242, 1250, 1408, 1898
TspGWI ACGGA 4 cut(s) 1139, 1398, 1981, 2287
TspRI CASTG 6 cut(s) 700, 1137, 1143, 1891, 1945, 2008
Tth111I GACNNNGTC 1 cut(s) 2070
Van91I CCANNNNNTGG 1 cut(s) 887
VpaK11BI GGWCC 3 cut(s) 1613, 1664, 2066
XapI RAATTY 3 cut(s) 759, 1117, 1638
XceI RCATGY 4 cut(s) 1536, 1763, 2064, 2129
XcmI CCANNNNNNNNNTGG 1 cut(s) 1883
XmiI GTMKAC 1 cut(s) 1102
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 4 cut(s) 1173, 1179, 1431, 2237
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.