Rw4G007750

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000579
Physical Location & Seq
Forward (+)
16685294 .. 16702100
16807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007750.1

Sequence Viewer

Length: 1356 bp
ATGCTATTAACAAACATTATATTAATAACATTTTCCCTTAATTTTTCTTTTTTACAGGGTCTGCTTTCGCTACTTACCTCTGTGTTGTTTTCATGGATTGTTGCTTCACCTAGATTTGGCATTGGGCCGCGTTATGGCCAAAACCGTACTCTTAACGTACTTGATTTTGGTGCCTCTGGTGATGGCATAGCAGATGATACTGAAGCTTTCTTAAATGCATGGAAGACACTTTGCGAGACTGGCCAAACCCTAAACATCCCCGCAGGGAAGACCTTTGTAGTCAAACCTCTTCAACTTGTAGGTCCATGCAAATCCAATAACGTTGGAATTCAGATTGATGGGAATATTGTCGGTCCATCCACTCTTAGCGAATGGGCAGACGCGTGCAAAATCGGACAATGTTGGCTATGCTTTCTAAACGTGAAGGGCCTCGTCATCAAGGGGGAAGGACTTATAAATGGCAATGGTCCAATTTGGTGGAAACAAGAAACCCAATCCATAAATGCTTTAAACTTCCACAAATGCGACCAACTTCAATTATCTGGATTTACTTCCCGTGATAGTCCGAGAGCCCATATATTTATACATAGCAGCAATGGTGTACGTATCTCAAACATTCACTTGAGTGCACCGGAAAAAAGCCCGAATACTGATGGCATTGACATCTCTTTATCAAGCCAAGTGACCATACAAGACTCTTTCATCGGAACTGGTGATGATTGCATTGCAATTAAGGGAGGCTCCTCTTTTGTAAACATAACTCATGTAACATGCGGACCAGGTCATGGCTTCAGTGTGGGTAGTTTAGGACAAGATCCAGCTATTACAGATAATGTCGAACAAGTACACTTTCAGAATTGTAGCTGCAACAAATCTACAAACTGTGCAAGAATCAAAACATGGATGGGTGGATCTGGATACGCTCGGGCGATTTTCTTCAGACAAATGATCCTTCAACAGTCACAAAATCCTATTATAATTGACCAACACTACTGCAATGGCGGACATAATTGCCCAGCTAAGACGGGAGCAGTGAAAGTTAGCAATGTAACATTCAGTGGCATTACCGGAACTTCAGCAAGTGAGCAAGCAATAACACTGGATTGCTCCGACTTGGGTTGTACCGATATCTTGATGGATCATGTCAACATAACCTCCGCATCTGGACTGCCACTTCATTCTGTGTGCAACAAGGCCAGTGGGAAAGCTAGTTTTACTTCACCTCCTATTCCTTGCCTGTCGTCGAATGGATCTTTGTCATCAGTGATTGCACCGATCTCGGTCAACTTATTAAAGCCAAACCATTCTTATTTGAGTTATTTCGGAATTCATGCCCATAAAACAATCGAAGCTTAA

Protein Analysis

451

Amino Acids

48.09

Weight (kDa)

6.89

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 51 - 276 3.9e-11 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 82 - 400 7e-79 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 977
AccB1I GGYRCC 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 785
AccII CGCG 2 cut(s) 130, 383
AciI CCGC 5 cut(s) 128, 261, 774, 1002, 1158
AclI AACGTT 1 cut(s) 321
AclWI GGATC 5 cut(s) 809, 919, 943, 1146, 1258
AcoI YGGCCR 2 cut(s) 136, 241
AcsI RAATTY 2 cut(s) 327, 1326
AcuI CTGAAG 4 cut(s) 222, 775, 922, 1059
AfaI GTAC 5 cut(s) 148, 159, 603, 846, 1123
AfiI CCNNNNNNNGG 3 cut(s) 116, 134, 785
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 3 cut(s) 293, 536, 956
AjnI CCWGG 1 cut(s) 778
AleI CACNNNNGTG 1 cut(s) 624
AluBI AGCT 6 cut(s) 206, 821, 864, 1019, 1208, 1352
AluI AGCT 6 cut(s) 206, 821, 864, 1019, 1208, 1352
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 1 cut(s) 230
Alw44I GTGCAC 1 cut(s) 627
AlwI GGATC 5 cut(s) 809, 919, 943, 1146, 1258
AlwNI CAGNNNCTG 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 924
AoxI GGCC 5 cut(s) 125, 136, 241, 427, 1194
ApaLI GTGCAC 1 cut(s) 627
ApeKI GCWGC 2 cut(s) 591, 864
ApoI RAATTY 2 cut(s) 327, 1326
AseI ATTAAT 1 cut(s) 23
AspS9I GGNCC 6 cut(s) 125, 302, 353, 427, 467, 776
AsuHPI GGTGA 4 cut(s) 99, 191, 725, 1212
AvaI CYCGRG 1 cut(s) 924
AvaII GGWCC 4 cut(s) 302, 353, 467, 776
BaeGI GKGCMC 1 cut(s) 631
BalI TGGCCA 2 cut(s) 138, 243
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 574
BbsI GAAGAC 2 cut(s) 230, 275
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 2 cut(s) 603, 851
BccI CCATC 6 cut(s) 176, 332, 364, 647, 898, 1129
BcgI CGANNNNNNTGC 2 cut(s) 1260, 1294
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 1 cut(s) 911
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 2 cut(s) 111, 1209
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 3 cut(s) 128, 592, 865
BlsI GCNGC 3 cut(s) 129, 593, 866
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 4 cut(s) 302, 353, 467, 776
BmeT110I CYCGRG 1 cut(s) 924
BmgT120I GGNCC 6 cut(s) 125, 302, 353, 427, 467, 776
BmiI GGNNCC 2 cut(s) 172, 742
BmrFI CCNGG 1 cut(s) 780
BmsI GCATC 1 cut(s) 1169
BpiI GAAGAC 2 cut(s) 230, 275
BpuEI CTTGAG 1 cut(s) 643
BsaAI YACGTR 1 cut(s) 605
BsaWI WCCGGW 2 cut(s) 631, 1067
BsaXI ACNNNNNCTCC 4 cut(s) 1139, 1169, 1207, 1237
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 134, 785
Bse1I ACTGG 4 cut(s) 244, 715, 1104, 1197
Bse3DI GCAATG 5 cut(s) 469, 601, 723, 1003, 1051
BseBI CCWGG 1 cut(s) 780
BseGI GGATG 3 cut(s) 255, 356, 909
BseLI CCNNNNNNNGG 3 cut(s) 116, 134, 785
BseMI GCAATG 5 cut(s) 469, 601, 723, 1003, 1051
BseNI ACTGG 4 cut(s) 244, 715, 1104, 1197
BseRI GAGGAG 1 cut(s) 733
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 2 cut(s) 603, 851
BseYI CCCAGC 1 cut(s) 1015
Bsh1236I CGCG 2 cut(s) 130, 383
BshFI GGCC 5 cut(s) 127, 138, 243, 429, 1196
BshNI GGYRCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 924
BsiSI CCGG 2 cut(s) 632, 1068
BslI CCNNNNNNNGG 3 cut(s) 116, 134, 785
BsmAI GTCTC 1 cut(s) 230
BsnI GGCC 5 cut(s) 127, 138, 243, 429, 1196
BsoBI CYCGRG 1 cut(s) 924
Bsp1286I GDGCHC 2 cut(s) 574, 631
Bsp143I GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
BspACI CCGC 5 cut(s) 128, 261, 774, 1002, 1158
BspANI GGCC 5 cut(s) 127, 138, 243, 429, 1196
BspFNI CGCG 2 cut(s) 130, 383
BspLI GGNNCC 2 cut(s) 172, 742
BspPI GGATC 5 cut(s) 809, 919, 943, 1146, 1258
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 5 cut(s) 469, 601, 723, 1003, 1051
BsrI ACTGG 4 cut(s) 244, 715, 1104, 1197
BssMI GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 3 cut(s) 146, 884, 960
Bst6I CTCTTC 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 2 cut(s) 385, 1089
BstDEI CTNAG 2 cut(s) 365, 1020
BstF5I GGATG 3 cut(s) 255, 356, 909
BstFNI CGCG 2 cut(s) 130, 383
BstKTI GATC 6 cut(s) 817, 914, 951, 1141, 1253, 1278
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
BstMWI GCNNNNNNNGC 1 cut(s) 240
BstNI CCWGG 1 cut(s) 780
BstNSI RCATGY 1 cut(s) 774
BstSCI CCNGG 1 cut(s) 778
BstSLI GKGCMC 1 cut(s) 631
BstSNI TACGTA 1 cut(s) 605
BstUI CGCG 2 cut(s) 130, 383
BstV1I GCAGC 2 cut(s) 603, 851
BstV2I GAAGAC 2 cut(s) 230, 275
BstX2I RGATCY 3 cut(s) 814, 911, 1250
BstXI CCANNNNNNTGG 1 cut(s) 477
BstYI RGATCY 3 cut(s) 814, 911, 1250
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 5 cut(s) 127, 138, 243, 429, 1196
BtsCI GGATG 3 cut(s) 255, 356, 909
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 6 cut(s) 799, 1038, 1063, 1097, 1204, 1269
Cac8I GCNNGC 2 cut(s) 385, 1089
CaiI CAGNNNCTG 1 cut(s) 61
Cfr13I GGNCC 6 cut(s) 125, 302, 353, 427, 467, 776
CseI GACGC 1 cut(s) 389
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 5 cut(s) 147, 158, 602, 845, 1122
CspCI CAANNNNNGTGG 2 cut(s) 1180, 1215
CviAII CATG 9 cut(s) 93, 219, 306, 764, 771, 785, 900, 1142, 1331
CviQI GTAC 5 cut(s) 147, 158, 602, 845, 1122
DdeI CTNAG 2 cut(s) 365, 1020
DpnI GATC 6 cut(s) 816, 913, 950, 1140, 1252, 1277
DpnII GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 2 cut(s) 136, 241
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
EciI GGCGGA 1 cut(s) 1017
Eco105I TACGTA 1 cut(s) 605
Eco24I GRGCYC 1 cut(s) 574
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 4 cut(s) 302, 353, 467, 776
Eco57I CTGAAG 4 cut(s) 222, 775, 922, 1059
Eco88I CYCGRG 1 cut(s) 924
EcoO109I RGGNCCY 1 cut(s) 427
EcoRI GAATTC 2 cut(s) 327, 1326
EcoRII CCWGG 1 cut(s) 778
EcoRV GATATC 1 cut(s) 1129
EcoT22I ATGCAT 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 574
FaeI CATG 9 cut(s) 96, 222, 309, 767, 774, 788, 903, 1145, 1334
FatI CATG 9 cut(s) 92, 218, 305, 763, 770, 784, 899, 1141, 1330
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 128, 592, 865
FokI GGATG 3 cut(s) 242, 343, 916
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 3 cut(s) 128, 592, 865
FspBI CTAG 2 cut(s) 111, 1209
GluI GCNGC 3 cut(s) 128, 592, 865
GsaI CCCAGC 1 cut(s) 1019
HaeIII GGCC 5 cut(s) 127, 138, 243, 429, 1196
HapII CCGG 2 cut(s) 632, 1068
HgaI GACGC 1 cut(s) 389
Hin1II CATG 9 cut(s) 96, 222, 309, 767, 774, 788, 903, 1145, 1334
HincII GTYRAC 2 cut(s) 1147, 1285
HindII GTYRAC 2 cut(s) 1147, 1285
HindIII AAGCTT 2 cut(s) 204, 1350
HinfI GANTC 2 cut(s) 695, 891
HpaII CCGG 2 cut(s) 632, 1068
HphI GGTGA 4 cut(s) 99, 191, 725, 1212
Hpy166II GTNNAC 6 cut(s) 602, 629, 754, 847, 1147, 1285
Hpy188I TCNGA 8 cut(s) 333, 395, 567, 707, 855, 941, 1111, 1325
Hpy188III TCNNGA 4 cut(s) 543, 915, 1132, 1164
Hpy8I GTNNAC 6 cut(s) 602, 629, 754, 847, 1147, 1285
Hpy99I CGWCG 1 cut(s) 1246
HpyAV CCTTC 3 cut(s) 418, 440, 962
HpyCH4III ACNGT 3 cut(s) 146, 884, 960
HpyCH4IV ACGT 4 cut(s) 156, 321, 420, 604
HpyF10VI GCNNNNNNNGC 1 cut(s) 240
HpyF3I CTNAG 2 cut(s) 365, 1020
HpySE526I ACGT 4 cut(s) 156, 321, 420, 604
Hsp92II CATG 9 cut(s) 96, 222, 309, 767, 774, 788, 903, 1145, 1334
Kzo9I GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
LmnI GCTCC 3 cut(s) 746, 1028, 1112
Lsp1109I GCAGC 2 cut(s) 603, 851
LweI GCATC 1 cut(s) 1169
MabI ACCWGGT 1 cut(s) 778
MaeI CTAG 2 cut(s) 111, 1209
MaeII ACGT 4 cut(s) 156, 321, 420, 604
MaeIII GTNAC 4 cut(s) 682, 766, 960, 1048
MalI GATC 6 cut(s) 816, 913, 950, 1140, 1252, 1277
MboI GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
MboII GAAGA 4 cut(s) 235, 280, 281, 928
MflI RGATCY 3 cut(s) 814, 911, 1250
MhlI GDGCHC 2 cut(s) 574, 631
MlsI TGGCCA 2 cut(s) 138, 243
MluCI AATT 9 cut(s) 40, 327, 471, 536, 729, 856, 978, 1009, 1326
MluI ACGCGT 1 cut(s) 381
MluNI TGGCCA 2 cut(s) 138, 243
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 2 cut(s) 304, 1134
MnlI CCTC 8 cut(s) 88, 184, 297, 440, 731, 754, 1165, 1233
Mox20I TGGCCA 2 cut(s) 138, 243
Mph1103I ATGCAT 1 cut(s) 220
MscI TGGCCA 2 cut(s) 138, 243
MseI TTAA 9 cut(s) 8, 23, 39, 153, 212, 509, 732, 1292, 1354
MslI CAYNNNNRTG 1 cut(s) 624
Msp20I TGGCCA 2 cut(s) 138, 243
MspI CCGG 2 cut(s) 632, 1068
MspR9I CCNGG 1 cut(s) 780
MvaI CCWGG 1 cut(s) 780
MvnI CGCG 2 cut(s) 130, 383
MwoI GCNNNNNNNGC 1 cut(s) 240
NdeII GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
NlaIII CATG 9 cut(s) 96, 222, 309, 767, 774, 788, 903, 1145, 1334
NlaIV GGNNCC 2 cut(s) 172, 742
NmuCI GTSAC 2 cut(s) 682, 960
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 774
OliI CACNNNNGTG 1 cut(s) 624
PfeI GAWTC 1 cut(s) 891
PflFI GACNNNGTC 1 cut(s) 780
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 3 cut(s) 129, 593, 866
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
Ppu21I YACGTR 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 23
PsiI TTATAA 2 cut(s) 455, 977
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 2 cut(s) 172, 742
PspPI GGNCC 6 cut(s) 125, 302, 353, 427, 467, 776
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 3 cut(s) 814, 911, 1250
PsyI GACNNNGTC 1 cut(s) 780
RsaI GTAC 5 cut(s) 148, 159, 603, 846, 1123
RsaNI GTAC 5 cut(s) 147, 158, 602, 845, 1122
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 9 cut(s) 8, 23, 39, 153, 212, 509, 732, 1292, 1354
SatI GCNGC 3 cut(s) 128, 592, 865
Sau3AI GATC 6 cut(s) 814, 911, 948, 1138, 1250, 1275
Sau96I GGNCC 6 cut(s) 125, 302, 353, 427, 467, 776
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 1 cut(s) 780
SduI GDGCHC 2 cut(s) 574, 631
SexAI ACCWGGT 1 cut(s) 778
SfaNI GCATC 1 cut(s) 1169
SinI GGWCC 4 cut(s) 302, 353, 467, 776
SmiMI CAYNNNNRTG 1 cut(s) 624
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
SnaBI TACGTA 1 cut(s) 605
Sse9I AATT 9 cut(s) 40, 327, 471, 536, 729, 856, 978, 1009, 1326
SsiI CCGC 5 cut(s) 128, 261, 774, 1002, 1158
SspI AATATT 1 cut(s) 346
SspMI CTAG 2 cut(s) 111, 1209
StyD4I CCNGG 1 cut(s) 778
TaaI ACNGT 3 cut(s) 146, 884, 960
TaiI ACGT 4 cut(s) 159, 324, 423, 607
TaqI TCGA 3 cut(s) 837, 1244, 1347
TaqII GACCGA 2 cut(s) 341, 1270
TasI AATT 9 cut(s) 40, 327, 471, 536, 729, 856, 978, 1009, 1326
TatI WGTACW 1 cut(s) 844
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 1 cut(s) 891
Tru1I TTAA 9 cut(s) 8, 23, 39, 153, 212, 509, 732, 1292, 1354
Tru9I TTAA 9 cut(s) 8, 23, 39, 153, 212, 509, 732, 1292, 1354
TscAI CASTG 6 cut(s) 799, 1038, 1063, 1104, 1204, 1269
TseFI GTSAC 2 cut(s) 682, 960
TseI GCWGC 2 cut(s) 591, 864
Tsp45I GTSAC 2 cut(s) 682, 960
TspDTI ATGAA 4 cut(s) 81, 691, 1166, 1319
TspRI CASTG 6 cut(s) 799, 1038, 1063, 1104, 1204, 1269
Tth111I GACNNNGTC 1 cut(s) 780
Van91I CCANNNNNTGG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 627
VpaK11BI GGWCC 4 cut(s) 302, 353, 467, 776
VspI ATTAAT 1 cut(s) 23
XapI RAATTY 2 cut(s) 327, 1326
XceI RCATGY 1 cut(s) 774
XspI CTAG 2 cut(s) 111, 1209
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.