Rroxscaffold_2G00100180

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000579
Physical Location & Seq
Forward (+)
22056716 .. 22060067
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100180.1

Sequence Viewer

Length: 1179 bp
ATGAGTAGCGTACTTGTTAGCTGTTTGTTATTTTGCATTCTTGCTTCATCAGATTTTAGAATTGGTTATGGCCAAACGACTTATAATGTACTCGACCATGGTGCGGTTGGAGATGGCCAAAGAGATGATTCTGAAGCTTTCTTGAAAGCATGGAACGCCGTATGTCTAGATAAAGGGACACCAAGACTTGTAATACCAGGGGAGAAGACATTTTTACTTCAACCTACCAAATTCTCAGGTCCTTGCCAGTCCAGCTCCATTCAGGTTGAGATTTCGGGAAAAATTGTGGCACCCAGTCCAGATGCATGGAAAGAGTGTGCAAACCCTTGGCTAGCCTTCTCACAGGTGACCAACCTCATCGTTAGTGGATCAGGAGAAATCAATGGCCAAGGTTCCTCTTGGTGGACAAAATATGACCTACTTAACTATGCTTTAGGTTTCTGCCGATGCGATAATCTTCAATTAAGTGGACTTACTCATGTTGACAGTCCCAAAGCACATATTTCCATACAATCTTGCAACAATGCGACTATCTCCTACCTTACTATTAGGGCACCTGAGGATAGTCCGAACACAGATGGAATGGACATATCTATGTCAAGCCATGTCAATATTCATGATTGCAATATTGGAACCGGTGATGATTGTATCGCCATTAATAATGGGTCATTCTATATCAATATCAACAACATCCAATGCGGACCAGGTCATGGAATTAGCATTGGAAGCTTAGGCCAAAATGGAGCTTATGAAACAGTAGAAGAGATACATGTACAAAATTGCACTTTCACTGCTACAATGTATGGAGCCAGAATCAAGACATATCAGGGTGGTTCCGGATATGCTAGAAAGATATCGTTTGAACAAATCGCCCTTAGAACTACAAGGAACCCCATTATTATTGACCAATTCTACTGTAATGGTCAACATGACTGCCAAACTTCGACTTCGGCCGTACGGGTGAGTGAAGTGACATACAATGGATTTCATGGAACTTCTGAGAACGAGCAAGCAATTACATTTAATTGCAGCCAAAGTATAGGTTGTGCCAACATTGTGATGAATCAAGTGAGTATCACCTCACCAGTTCCCAGTGAGCAGGTTAGGGCTTCTTGCAGCAATGTAAATGGGACTTCTCACGAAACTATACCACAAGTCCCTTGCCTAAAAAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

392

Amino Acids

42.3

Weight (kDa)

5.31

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 376 8.2e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 1090
AccB1I GGYRCC 2 cut(s) 289, 553
AccB7I CCANNNNNTGG 1 cut(s) 710
AccIII TCCGGA 1 cut(s) 836
AciI CCGC 2 cut(s) 104, 699
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 4 cut(s) 70, 115, 385, 951
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 4 cut(s) 12, 90, 774, 957
AfiI CCNNNNNNNGG 3 cut(s) 103, 402, 710
AflIII ACRYGT 1 cut(s) 769
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 4 cut(s) 145, 221, 461, 863
AjnI CCWGG 2 cut(s) 196, 703
AluBI AGCT 5 cut(s) 21, 137, 255, 729, 746
AluI AGCT 5 cut(s) 21, 137, 255, 729, 746
AlwI GGATC 1 cut(s) 376
Aor13HI TCCGGA 1 cut(s) 836
AoxI GGCC 5 cut(s) 70, 115, 385, 733, 951
ApeKI GCWGC 2 cut(s) 1029, 1116
ApoI RAATTY 1 cut(s) 230
AseI ATTAAT 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 635
AspS9I GGNCC 2 cut(s) 239, 701
AsuHPI GGTGA 5 cut(s) 358, 650, 973, 1069, 1074
AsuNHI GCTAGC 1 cut(s) 331
AvaII GGWCC 2 cut(s) 239, 701
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 556
BalI TGGCCA 3 cut(s) 72, 117, 387
BanI GGYRCC 2 cut(s) 289, 553
BbsI GAAGAC 1 cut(s) 212
BbvI GCAGC 2 cut(s) 1041, 1128
BccI CCATC 2 cut(s) 107, 572
BceAI ACGGC 2 cut(s) 143, 938
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 198, 705
BfaI CTAG 3 cut(s) 167, 332, 846
BfuAI ACCTGC 1 cut(s) 1090
BisI GCNGC 2 cut(s) 1030, 1117
BlsI GCNGC 2 cut(s) 1031, 1118
Bme1390I CCNGG 2 cut(s) 198, 705
Bme18I GGWCC 2 cut(s) 239, 701
BmgT120I GGNCC 2 cut(s) 239, 701
BmiI GGNNCC 7 cut(s) 291, 394, 555, 634, 808, 835, 890
BmrFI CCNGG 2 cut(s) 198, 705
BmrI ACTGGG 2 cut(s) 288, 1086
BmsI GCATC 2 cut(s) 292, 437
BmtI GCTAGC 1 cut(s) 335
BmuI ACTGGG 2 cut(s) 288, 1086
BpiI GAAGAC 1 cut(s) 212
Bpu10I CCTNAGC 1 cut(s) 730
BsaJI CCNNGG 4 cut(s) 97, 197, 326, 388
BsaWI WCCGGW 2 cut(s) 635, 836
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 402, 710
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 4 cut(s) 247, 294, 1085, 1092
Bse21I CCTNAGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 1126
BseAI TCCGGA 1 cut(s) 836
BseBI CCWGG 2 cut(s) 198, 705
BseDI CCNNGG 4 cut(s) 97, 197, 326, 388
BseGI GGATG 1 cut(s) 690
BseLI CCNNNNNNNGG 3 cut(s) 103, 402, 710
BseMI GCAATG 1 cut(s) 1126
BseMII CTCAG 3 cut(s) 249, 549, 990
BseNI ACTGG 4 cut(s) 247, 294, 1085, 1092
BseSI GKGCMC 1 cut(s) 556
BseX3I CGGCCG 1 cut(s) 951
BseXI GCAGC 2 cut(s) 1041, 1128
Bsh1285I CGRYCG 1 cut(s) 954
BshFI GGCC 5 cut(s) 72, 117, 387, 735, 953
BshNI GGYRCC 2 cut(s) 289, 553
BshTI ACCGGT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 954
BsiSI CCGG 2 cut(s) 636, 837
BsiWI CGTACG 1 cut(s) 955
BslFI GGGAC 4 cut(s) 190, 474, 1142, 1144
BslI CCNNNNNNNGG 3 cut(s) 103, 402, 710
BsmFI GGGAC 4 cut(s) 190, 474, 1142, 1144
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 5 cut(s) 72, 117, 387, 735, 953
Bsp1286I GDGCHC 1 cut(s) 556
Bsp13I TCCGGA 1 cut(s) 836
Bsp1407I TGTACA 1 cut(s) 772
Bsp143I GATC 1 cut(s) 368
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 104, 699
BspANI GGCC 5 cut(s) 72, 117, 387, 735, 953
BspCNI CTCAG 3 cut(s) 248, 550, 991
BspEI TCCGGA 1 cut(s) 836
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 7 cut(s) 291, 394, 555, 634, 808, 835, 890
BspMI ACCTGC 1 cut(s) 1090
BspOI GCTAGC 1 cut(s) 335
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 2 cut(s) 289, 553
BsrDI GCAATG 1 cut(s) 1126
BsrFI RCCGGY 1 cut(s) 635
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 4 cut(s) 247, 294, 1085, 1092
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 4 cut(s) 97, 197, 326, 388
BssMI GATC 1 cut(s) 368
BssT1I CCWWGG 3 cut(s) 97, 326, 388
Bst2UI CCWGG 2 cut(s) 198, 705
Bst4CI ACNGT 3 cut(s) 488, 757, 917
Bst6I CTCTTC 1 cut(s) 756
BstAUI TGTACA 1 cut(s) 772
BstC8I GCNNGC 2 cut(s) 333, 1011
BstDEI CTNAG 5 cut(s) 235, 558, 730, 875, 999
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 346
BstF5I GGATG 1 cut(s) 690
BstKTI GATC 1 cut(s) 371
BstMBI GATC 1 cut(s) 368
BstMCI CGRYCG 1 cut(s) 954
BstMWI GCNNNNNNNGC 3 cut(s) 155, 252, 726
BstNI CCWGG 2 cut(s) 198, 705
BstNSI RCATGY 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 196, 703
BstSLI GKGCMC 1 cut(s) 556
BstV1I GCAGC 2 cut(s) 1041, 1128
BstV2I GAAGAC 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 306
BstZI CGGCCG 1 cut(s) 951
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 5 cut(s) 72, 117, 387, 735, 953
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 1 cut(s) 690
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 789, 1099
BveI ACCTGC 1 cut(s) 1090
Cac8I GCNNGC 2 cut(s) 333, 1011
CciI TCATGA 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 2 cut(s) 239, 701
CsiI ACCWGGT 1 cut(s) 703
Csp6I GTAC 4 cut(s) 11, 89, 773, 956
CspAI ACCGGT 1 cut(s) 635
CviQI GTAC 4 cut(s) 11, 89, 773, 956
DdeI CTNAG 5 cut(s) 235, 558, 730, 875, 999
DpnI GATC 1 cut(s) 370
DpnII GATC 1 cut(s) 368
EaeI YGGCCR 4 cut(s) 70, 115, 385, 951
EagI CGGCCG 1 cut(s) 951
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
EclXI CGGCCG 1 cut(s) 951
Eco130I CCWWGG 3 cut(s) 97, 326, 388
Eco32I GATATC 1 cut(s) 855
Eco47I GGWCC 2 cut(s) 239, 701
Eco52I CGGCCG 1 cut(s) 951
Eco57I CTGAAG 1 cut(s) 153
Eco81I CCTNAGG 1 cut(s) 558
Eco91I GGTNACC 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 196, 703
EcoRV GATATC 1 cut(s) 855
EcoT14I CCWWGG 3 cut(s) 97, 326, 388
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 3 cut(s) 97, 326, 388
FaqI GGGAC 4 cut(s) 190, 474, 1142, 1144
Fnu4HI GCNGC 2 cut(s) 1030, 1117
FokI GGATG 1 cut(s) 677
Fsp4HI GCNGC 2 cut(s) 1030, 1117
FspBI CTAG 3 cut(s) 167, 332, 846
GluI GCNGC 2 cut(s) 1030, 1117
HaeIII GGCC 5 cut(s) 72, 117, 387, 735, 953
HapII CCGG 2 cut(s) 636, 837
HincII GTYRAC 2 cut(s) 484, 926
HindII GTYRAC 2 cut(s) 484, 926
HindIII AAGCTT 2 cut(s) 135, 727
HinfI GANTC 3 cut(s) 128, 813, 1063
HpaII CCGG 2 cut(s) 636, 837
HphI GGTGA 5 cut(s) 358, 650, 973, 1069, 1074
Hpy166II GTNNAC 4 cut(s) 405, 470, 484, 926
Hpy188I TCNGA 4 cut(s) 52, 133, 570, 1000
Hpy188III TCNNGA 9 cut(s) 142, 167, 276, 299, 372, 617, 817, 837, 1139
Hpy8I GTNNAC 4 cut(s) 405, 470, 484, 926
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 3 cut(s) 488, 757, 917
HpyCH4V TGCA 8 cut(s) 36, 305, 320, 519, 624, 783, 1029, 1116
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 252, 726
HpyF3I CTNAG 5 cut(s) 235, 558, 730, 875, 999
Kpn2I TCCGGA 1 cut(s) 836
Kzo9I GATC 1 cut(s) 368
LmnI GCTCC 3 cut(s) 260, 743, 806
Lsp1109I GCAGC 2 cut(s) 1041, 1128
LweI GCATC 2 cut(s) 292, 437
MabI ACCWGGT 1 cut(s) 703
MaeI CTAG 3 cut(s) 167, 332, 846
MaeIII GTNAC 2 cut(s) 346, 970
MalI GATC 1 cut(s) 370
MboI GATC 1 cut(s) 368
MboII GAAGA 3 cut(s) 217, 449, 773
MhlI GDGCHC 1 cut(s) 556
MlsI TGGCCA 3 cut(s) 72, 117, 387
MluNI TGGCCA 3 cut(s) 72, 117, 387
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 4 cut(s) 365, 406, 553, 1090
Mox20I TGGCCA 3 cut(s) 72, 117, 387
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 1 cut(s) 836
MscI TGGCCA 3 cut(s) 72, 117, 387
MseI TTAA 5 cut(s) 423, 464, 657, 1023, 1177
MslI CAYNNNNRTG 2 cut(s) 593, 1058
Msp20I TGGCCA 3 cut(s) 72, 117, 387
MspI CCGG 2 cut(s) 636, 837
MspR9I CCNGG 2 cut(s) 198, 705
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 2 cut(s) 198, 705
MwoI GCNNNNNNNGC 3 cut(s) 155, 252, 726
NcoI CCATGG 1 cut(s) 97
NdeII GATC 1 cut(s) 368
NheI GCTAGC 1 cut(s) 331
NlaIV GGNNCC 7 cut(s) 291, 394, 555, 634, 808, 835, 890
NmuCI GTSAC 2 cut(s) 346, 970
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 1 cut(s) 773
PagI TCATGA 1 cut(s) 616
PciI ACATGT 1 cut(s) 769
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 128, 813, 1063
Pfl23II CGTACG 1 cut(s) 955
PflFI GACNNNGTC 1 cut(s) 705
PflMI CCANNNNNTGG 1 cut(s) 710
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 2 cut(s) 1031, 1118
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 769
PshBI ATTAAT 1 cut(s) 657
PsiI TTATAA 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 196, 703
PspEI GGTNACC 1 cut(s) 346
PspGI CCWGG 2 cut(s) 196, 703
PspLI CGTACG 1 cut(s) 955
PspN4I GGNNCC 7 cut(s) 291, 394, 555, 634, 808, 835, 890
PspPI GGNCC 2 cut(s) 239, 701
PspPPI RGGWCCY 1 cut(s) 239
PsyI GACNNNGTC 1 cut(s) 705
RsaI GTAC 4 cut(s) 12, 90, 774, 957
RsaNI GTAC 4 cut(s) 11, 89, 773, 956
RseI CAYNNNNRTG 2 cut(s) 593, 1058
SaqAI TTAA 5 cut(s) 423, 464, 657, 1023, 1177
SatI GCNGC 2 cut(s) 1030, 1117
Sau3AI GATC 1 cut(s) 368
Sau96I GGNCC 2 cut(s) 239, 701
ScrFI CCNGG 2 cut(s) 198, 705
SduI GDGCHC 1 cut(s) 556
SexAI ACCWGGT 1 cut(s) 703
SfaNI GCATC 2 cut(s) 292, 437
SinI GGWCC 2 cut(s) 239, 701
SmiMI CAYNNNNRTG 2 cut(s) 593, 1058
SsiI CCGC 2 cut(s) 104, 699
SspI AATATT 2 cut(s) 613, 628
SspMI CTAG 3 cut(s) 167, 332, 846
StyD4I CCNGG 2 cut(s) 196, 703
StyI CCWWGG 3 cut(s) 97, 326, 388
TaaI ACNGT 3 cut(s) 488, 757, 917
TaqI TCGA 2 cut(s) 93, 944
TatI WGTACW 2 cut(s) 88, 772
TfiI GAWTC 3 cut(s) 128, 813, 1063
Tru1I TTAA 5 cut(s) 423, 464, 657, 1023, 1177
Tru9I TTAA 5 cut(s) 423, 464, 657, 1023, 1177
TscAI CASTG 2 cut(s) 796, 1099
TseFI GTSAC 2 cut(s) 346, 970
TseI GCWGC 2 cut(s) 1029, 1116
Tsp45I GTSAC 2 cut(s) 346, 970
TspDTI ATGAA 5 cut(s) 36, 605, 765, 977, 1076
TspRI CASTG 2 cut(s) 796, 1099
Tth111I GACNNNGTC 1 cut(s) 705
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 239, 701
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 1 cut(s) 230
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
XspI CTAG 3 cut(s) 167, 332, 846
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.