RchiOBHm_Chr3g0481551

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000579
Physical Location & Seq
Reverse (-)
27587661 .. 27591974
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44650

Sequence Viewer

Length: 1263 bp
ATGAGAAAACTACTTGCAAGCTTTTTGATAGTTTGCATTTTTACTACATCGTCAAATTTTAATGCTGGGTATTGTCAAGAAACTTTTAATGTGCTTGACTACGGTGCTGTTGGAGATGGCCAAACCGATGATTCTGAAGCTTTCTTGACAGCATGGAGTGCTTTATGTGCAGCAAAAGGAACACCAGCACTTGTAATACCAATGGAAAAAACATTCCTTTTGCAACCTACAAAATTCTCAGGTCCTTGCATATCCAATGGCTCTATCTATGTTGAGATTTTGGGGAAAATTGTGGCACCCAAAACTCCTCAAGAATGGAAAGAGTGTGAAAAAGAATCCTGGCTGCTTTTTTCAGAGGTGAACAACCTAATCATTAATGGAAATGAAACAGGAGTAATCAATGGGAATGGTTTACCTTGGTGGACAGAGGCTCTACGCTTCTTCAATTGTAATGATCTTCAGTTAAGTGGACTCACACACGTTGATAGTCCGAAAGGGCATATTAGCATACTAAACTGCACCAATGTTATAGTATCCAATCTTCATATCATTGCACCAGATGATAGTGAAAACACAGATGGCATTGATATCAATATGTCAAGACATGTCAATATTCATGACTGCATAATTGGAACAGGAGACGATTGCATTGCTATTAAACGTGGCTCAGTCCTTGTCAATGTCACCAACATTGCTTGTGGACCAGGTCATGGAATTAGTGTGGGAAGCATAGGAAAATTAGAAGGAGCTTATGAGACAGTCGCAGAGGTTTTTGTACGAAATTGTACCTTCAATGGTACGATGAATGGAGCTAGAATCAAGACATGGCCCGGTGGTTCTGGATATGCCAAGAATATCTCGTATGAGAAAATCACTCTAATAGGAGTTCAAAATCCTATTATTATTGATCAAAACTACTGCAATGGTGAACACGACTGCGGTAATATAACGTCGAATTCAGCCGTGGAGGTGAGTAATGTGATATTCAATGACTTTCGAGGAACTCTGGCGGATGAACATGCGATTACATTTGATTGCTCCAAGACTGAATATGGCACTCTAGCTTGTCTCAACATTAATATGGAGCAAATCAACATTACTTCCATAGATCCCGAAAAGGAAATCTCCGTCTTATGCAAGAATGTAAATGGAACTACTGAAGATACCAGTCCCAGTGTTCATTGCCTACAAGCTAGAAATTCAACCATTGATGCAGATAGTCCTGATGCAGATAGTCCTGATCCCTACGCCTATTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

420

Amino Acids

45.43

Weight (kDa)

4.61

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 383 1.2e-72 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 2 cut(s) 939, 1010
AclWI GGATC 2 cut(s) 1103, 1235
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 4 cut(s) 55, 233, 955, 1198
AcuI CTGAAG 3 cut(s) 156, 443, 1179
AfaI GTAC 3 cut(s) 777, 787, 799
AfiI CCNNNNNNNGG 1 cut(s) 710
AflIII ACRYGT 2 cut(s) 478, 604
AgsI TTSAA 5 cut(s) 445, 793, 890, 988, 1203
AjnI CCWGG 2 cut(s) 338, 703
AluBI AGCT 6 cut(s) 21, 140, 749, 812, 1064, 1193
AluI AGCT 6 cut(s) 21, 140, 749, 812, 1064, 1193
Alw26I GTCTC 3 cut(s) 633, 749, 1073
AlwI GGATC 2 cut(s) 1103, 1235
AoxI GGCC 2 cut(s) 118, 827
ApeKI GCWGC 2 cut(s) 170, 343
ApoI RAATTY 4 cut(s) 55, 233, 955, 1198
AseI ATTAAT 2 cut(s) 375, 1077
AspS9I GGNCC 3 cut(s) 242, 701, 828
AsuC2I CCSGG 1 cut(s) 831
AsuHPI GGTGA 4 cut(s) 370, 676, 938, 982
AvaII GGWCC 2 cut(s) 242, 701
BalI TGGCCA 1 cut(s) 120
BanI GGYRCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
BbvI GCAGC 2 cut(s) 182, 330
BccI CCATC 2 cut(s) 110, 572
BceAI ACGGC 1 cut(s) 947
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciT130I CCWGG 2 cut(s) 340, 705
BciVI GTATCC 1 cut(s) 544
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 1 cut(s) 831
BcoDI GTCTC 3 cut(s) 633, 749, 1073
BfaI CTAG 3 cut(s) 813, 1061, 1194
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 2 cut(s) 171, 344
BlsI GCNGC 2 cut(s) 172, 345
Bme1390I CCNGG 3 cut(s) 340, 705, 831
Bme18I GGWCC 2 cut(s) 242, 701
BmgT120I GGNCC 3 cut(s) 242, 701, 828
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 3 cut(s) 340, 705, 831
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 2 cut(s) 1201, 1216
BmuI ACTGGG 1 cut(s) 1167
BpuEI CTTGAG 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 416, 963
Bsc4I CCNNNNNNNGG 1 cut(s) 710
Bse1I ACTGG 2 cut(s) 1167, 1173
Bse3DI GCAATG 5 cut(s) 549, 648, 690, 928, 1180
BseBI CCWGG 2 cut(s) 340, 705
BseDI CCNNGG 2 cut(s) 416, 963
BseGI GGATG 1 cut(s) 1018
BseLI CCNNNNNNNGG 1 cut(s) 710
BseMI GCAATG 5 cut(s) 549, 648, 690, 928, 1180
BseMII CTCAG 2 cut(s) 252, 681
BseNI ACTGG 2 cut(s) 1167, 1173
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 182, 330
BseYI CCCAGC 1 cut(s) 65
BsgI GTGCAG 2 cut(s) 189, 502
BshFI GGCC 2 cut(s) 120, 829
BshNI GGYRCC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 831
BslFI GGGAC 1 cut(s) 1155
BslI CCNNNNNNNGG 1 cut(s) 710
BsmAI GTCTC 3 cut(s) 633, 749, 1073
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 1155
BsnI GGCC 2 cut(s) 120, 829
Bsp143I GATC 4 cut(s) 454, 907, 1108, 1240
BspACI CCGC 2 cut(s) 939, 1010
BspANI GGCC 2 cut(s) 120, 829
BspCNI CTCAG 2 cut(s) 251, 680
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 297
BspPI GGATC 2 cut(s) 1103, 1235
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 5 cut(s) 549, 648, 690, 928, 1180
BsrI ACTGG 2 cut(s) 1167, 1173
BssECI CCNNGG 2 cut(s) 416, 963
BssMI GATC 4 cut(s) 454, 907, 1108, 1240
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 340, 705
Bst4CI ACNGT 2 cut(s) 104, 760
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 238, 667
BstDSI CCRYGG 1 cut(s) 963
BstF5I GGATG 1 cut(s) 1018
BstKTI GATC 4 cut(s) 457, 910, 1111, 1243
BstMAI GTCTC 3 cut(s) 633, 749, 1073
BstMBI GATC 4 cut(s) 454, 907, 1108, 1240
BstMWI GCNNNNNNNGC 2 cut(s) 158, 167
BstNI CCWGG 2 cut(s) 340, 705
BstNSI RCATGY 2 cut(s) 608, 1022
BstSCI CCNGG 3 cut(s) 338, 703, 829
BstV1I GCAGC 2 cut(s) 182, 330
BstX2I RGATCY 1 cut(s) 1108
BstYI RGATCY 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 120, 829
BtgI CCRYGG 1 cut(s) 963
BtsCI GGATG 1 cut(s) 1018
BtsIMutI CAGTG 1 cut(s) 1180
Cac8I GCNNGC 1 cut(s) 19
CciI TCATGA 1 cut(s) 616
Cfr13I GGNCC 3 cut(s) 242, 701, 828
CsiI ACCWGGT 1 cut(s) 703
Csp6I GTAC 3 cut(s) 776, 786, 798
CviAII CATG 6 cut(s) 153, 605, 617, 710, 825, 1019
CviQI GTAC 3 cut(s) 776, 786, 798
DdeI CTNAG 2 cut(s) 238, 667
DpnI GATC 4 cut(s) 456, 909, 1110, 1242
DpnII GATC 4 cut(s) 454, 907, 1108, 1240
EaeI YGGCCR 1 cut(s) 118
EciI GGCGGA 1 cut(s) 1025
Eco130I CCWWGG 1 cut(s) 416
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 242, 701
Eco57I CTGAAG 3 cut(s) 156, 443, 1179
EcoO109I RGGNCCY 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 955
EcoRII CCWGG 2 cut(s) 338, 703
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 416
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 6 cut(s) 156, 608, 620, 713, 828, 1022
FaqI GGGAC 1 cut(s) 1155
FatI CATG 6 cut(s) 152, 604, 616, 709, 824, 1018
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 171, 344
FokI GGATG 1 cut(s) 1025
Fsp4HI GCNGC 2 cut(s) 171, 344
FspBI CTAG 3 cut(s) 813, 1061, 1194
GluI GCNGC 2 cut(s) 171, 344
GsaI CCCAGC 1 cut(s) 69
HaeIII GGCC 2 cut(s) 120, 829
HapII CCGG 1 cut(s) 831
Hin1II CATG 6 cut(s) 156, 608, 620, 713, 828, 1022
HindIII AAGCTT 2 cut(s) 19, 138
HinfI GANTC 4 cut(s) 131, 335, 471, 816
HpaII CCGG 1 cut(s) 831
HphI GGTGA 4 cut(s) 370, 676, 938, 982
Hpy166II GTNNAC 6 cut(s) 361, 413, 423, 470, 701, 929
Hpy188I TCNGA 3 cut(s) 136, 355, 492
Hpy8I GTNNAC 6 cut(s) 361, 413, 423, 470, 701, 929
Hpy99I CGWCG 1 cut(s) 955
HpyAV CCTTC 2 cut(s) 737, 799
HpyCH4III ACNGT 2 cut(s) 104, 760
HpyCH4IV ACGT 3 cut(s) 480, 661, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 167
HpyF3I CTNAG 2 cut(s) 238, 667
HpySE526I ACGT 3 cut(s) 480, 661, 950
Hsp92II CATG 6 cut(s) 156, 608, 620, 713, 828, 1022
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 4 cut(s) 454, 907, 1108, 1240
LmnI GCTCC 4 cut(s) 746, 809, 1043, 1084
Lsp1109I GCAGC 2 cut(s) 182, 330
LweI GCATC 2 cut(s) 1201, 1216
MabI ACCWGGT 1 cut(s) 703
MaeI CTAG 3 cut(s) 813, 1061, 1194
MaeII ACGT 3 cut(s) 480, 661, 950
MaeIII GTNAC 1 cut(s) 682
MalI GATC 4 cut(s) 456, 909, 1110, 1242
MboI GATC 4 cut(s) 454, 907, 1108, 1240
MboII GAAGA 4 cut(s) 433, 449, 533, 1172
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 1 cut(s) 1108
MlsI TGGCCA 1 cut(s) 120
MluNI TGGCCA 1 cut(s) 120
MlyI GAGTC 1 cut(s) 465
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 6 cut(s) 318, 349, 421, 760, 961, 992
Mox20I TGGCCA 1 cut(s) 120
MscI TGGCCA 1 cut(s) 120
MseI TTAA 6 cut(s) 60, 87, 375, 464, 657, 1077
MslI CAYNNNNRTG 1 cut(s) 1079
Msp20I TGGCCA 1 cut(s) 120
MspI CCGG 1 cut(s) 831
MspR9I CCNGG 3 cut(s) 340, 705, 831
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 2 cut(s) 340, 705
MwoI GCNNNNNNNGC 2 cut(s) 158, 167
NciI CCSGG 1 cut(s) 831
NdeII GATC 4 cut(s) 454, 907, 1108, 1240
NlaIII CATG 6 cut(s) 156, 608, 620, 713, 828, 1022
NlaIV GGNNCC 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 2 cut(s) 608, 1022
PagI TCATGA 1 cut(s) 616
PciI ACATGT 1 cut(s) 604
PfeI GAWTC 3 cut(s) 131, 335, 816
PflFI GACNNNGTC 1 cut(s) 705
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 2 cut(s) 172, 345
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PpuMI RGGWCCY 1 cut(s) 242
PscI ACATGT 1 cut(s) 604
PshBI ATTAAT 2 cut(s) 375, 1077
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 338, 703
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 2 cut(s) 338, 703
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 3 cut(s) 242, 701, 828
PspPPI RGGWCCY 1 cut(s) 242
PsuI RGATCY 1 cut(s) 1108
PsyI GACNNNGTC 1 cut(s) 705
RsaI GTAC 3 cut(s) 777, 787, 799
RsaNI GTAC 3 cut(s) 776, 786, 798
RseI CAYNNNNRTG 1 cut(s) 1079
SaqAI TTAA 6 cut(s) 60, 87, 375, 464, 657, 1077
SatI GCNGC 2 cut(s) 171, 344
Sau3AI GATC 4 cut(s) 454, 907, 1108, 1240
Sau96I GGNCC 3 cut(s) 242, 701, 828
SchI GAGTC 1 cut(s) 465
ScrFI CCNGG 3 cut(s) 340, 705, 831
SexAI ACCWGGT 1 cut(s) 703
SfaNI GCATC 2 cut(s) 1201, 1216
SinI GGWCC 2 cut(s) 242, 701
SmiMI CAYNNNNRTG 1 cut(s) 1079
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SsiI CCGC 2 cut(s) 939, 1010
SspI AATATT 1 cut(s) 613
SspMI CTAG 3 cut(s) 813, 1061, 1194
StyD4I CCNGG 3 cut(s) 338, 703, 829
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 2 cut(s) 104, 760
TaiI ACGT 3 cut(s) 483, 664, 953
TaqI TCGA 2 cut(s) 953, 997
TfiI GAWTC 3 cut(s) 131, 335, 816
Tru1I TTAA 6 cut(s) 60, 87, 375, 464, 657, 1077
Tru9I TTAA 6 cut(s) 60, 87, 375, 464, 657, 1077
TscAI CASTG 1 cut(s) 1180
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 2 cut(s) 170, 343
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 6 cut(s) 399, 533, 605, 818, 1029, 1169
TspGWI ACGGA 1 cut(s) 1117
TspRI CASTG 1 cut(s) 1180
Tth111I GACNNNGTC 1 cut(s) 705
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 242, 701
VspI ATTAAT 2 cut(s) 375, 1077
XapI RAATTY 4 cut(s) 55, 233, 955, 1198
XceI RCATGY 2 cut(s) 608, 1022
XspI CTAG 3 cut(s) 813, 1061, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.