AT5G35695

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000697
Physical Location & Seq
Forward (+)
13869120 .. 13869941
822 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35695.1

Sequence Viewer

Length: 636 bp
ATGGAAAGAGATACATCTCGTAAAGCTAATATGGAAATGAGTAGCTTTGCTTGGGGATATGCAACATGGATATTTATCTATGTTCTGAGTGGATGGGAGGGTTCGGCTCACGATTCAAGAGTGTTAAGTGATGCATTAAGAAAATTCTATCTGGTTGATTGTGGATTTGCAAATCGTCTCAATTTCTTAGCTCCATTTCGCGGAGTTAGATATCATCTTCAGGAATTTGCTGGTCAAAGGCGCGATCCTGAAACTCCACATGAGTTATTCAATCTTCGTCATGTTTCTTTGAGAAACGTAATAGAAAGGATATTTGGAATCTTTAAATCTCGGTTTGCTATTTTTAAATCTGCTCCTCCTTTTTCTTACAAGAAACAAGCTGGATTAGTCTTAACATGTGCAGCGTTGCATAACTTTCTTCGTAAAGAATGTCGATCAGATGAAGCCGACTTCCCTGATGAAGTGGGAAATGAAGGTGACGTAGTTAACAATGAAGGTAATGCAATGAACACCAATGAAATTGATAATGAGGAACCTCTCGAAGCACAAAAACAAGACAGAGAAAACACAAATATGTGGAGAAAATCTATGGCTGAAGACATGTGGAAAGATGCCACAAATTTGGAAATTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

211

Amino Acids

24.51

Weight (kDa)

5.73

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 24 - 138 6.6e-12 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 201, 243
AciI CCGC 1 cut(s) 201
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 4 cut(s) 143, 224, 619, 627
AcuI CTGAAG 2 cut(s) 203, 615
AfiI CCNNNNNNNGG 1 cut(s) 200
AflIII ACRYGT 2 cut(s) 395, 600
AgsI TTSAA 2 cut(s) 117, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 4 cut(s) 26, 45, 191, 380
AluI AGCT 4 cut(s) 26, 45, 191, 380
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 4 cut(s) 143, 224, 619, 627
AspLEI GCGC 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 413
BccI CCATC 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 534
BmsI GCATC 2 cut(s) 121, 601
BpiI GAAGAC 1 cut(s) 603
BsaBI GATNNNNATC 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 74
BseGI GGATG 1 cut(s) 98
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 345
BseXI GCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 201, 243
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 182
BsmBI CGTCTC 1 cut(s) 182
Bsp143I GATC 2 cut(s) 244, 434
BspACI CCGC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 2 cut(s) 201, 243
BspLI GGNNCC 1 cut(s) 534
BspPI GGATC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 510
BssMI GATC 2 cut(s) 244, 434
BstDEI CTNAG 2 cut(s) 86, 187
BstF5I GGATG 1 cut(s) 98
BstFNI CGCG 2 cut(s) 201, 243
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 2 cut(s) 247, 437
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 244, 434
BstNSI RCATGY 2 cut(s) 399, 604
BstUI CGCG 2 cut(s) 201, 243
BstV1I GCAGC 1 cut(s) 413
BstV2I GAAGAC 1 cut(s) 603
BstXI CCANNNNNNTGG 1 cut(s) 622
BtsCI GGATG 1 cut(s) 98
CfoI GCGC 1 cut(s) 243
CviAII CATG 5 cut(s) 66, 260, 281, 396, 601
CviJI RGCY 7 cut(s) 26, 45, 107, 191, 380, 446, 593
CviKI_1 RGCY 7 cut(s) 26, 45, 107, 191, 380, 446, 593
DdeI CTNAG 2 cut(s) 86, 187
DpnI GATC 2 cut(s) 246, 436
DpnII GATC 2 cut(s) 244, 434
DraI TTTAAA 2 cut(s) 325, 346
Eco32I GATATC 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 203, 615
EcoRV GATATC 1 cut(s) 212
EcoT22I ATGCAT 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 5 cut(s) 69, 263, 284, 399, 604
FatI CATG 5 cut(s) 65, 259, 280, 395, 600
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 402
GlaI GCGC 1 cut(s) 242
GluI GCNGC 1 cut(s) 402
HhaI GCGC 1 cut(s) 243
Hin1II CATG 5 cut(s) 69, 263, 284, 399, 604
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 2 cut(s) 113, 318
HpaI GTTAAC 1 cut(s) 487
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 2 cut(s) 87, 439
Hpy188III TCNNGA 5 cut(s) 110, 117, 221, 248, 539
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 2 cut(s) 467, 488
HpyCH4IV ACGT 2 cut(s) 297, 480
HpyCH4V TGCA 6 cut(s) 62, 134, 170, 401, 409, 503
HpyF3I CTNAG 2 cut(s) 86, 187
HpySE526I ACGT 2 cut(s) 297, 480
Hsp92II CATG 5 cut(s) 69, 263, 284, 399, 604
HspAI GCGC 1 cut(s) 241
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 2 cut(s) 244, 434
LmnI GCTCC 2 cut(s) 196, 358
LpnPI CCDG 6 cut(s) 137, 206, 216, 261, 366, 468
Lsp1109I GCAGC 1 cut(s) 413
LweI GCATC 2 cut(s) 121, 601
MaeII ACGT 2 cut(s) 297, 480
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 246, 436
MboI GATC 2 cut(s) 244, 434
MboII GAAGA 4 cut(s) 209, 266, 410, 608
MluCI AATT 6 cut(s) 143, 181, 224, 519, 619, 627
MnlI CCTC 4 cut(s) 91, 366, 523, 546
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 6 cut(s) 125, 137, 324, 345, 392, 486
MslI CAYNNNNRTG 1 cut(s) 572
MvnI CGCG 2 cut(s) 201, 243
NdeII GATC 2 cut(s) 244, 434
NlaIII CATG 5 cut(s) 69, 263, 284, 399, 604
NlaIV GGNNCC 1 cut(s) 534
NmuCI GTSAC 1 cut(s) 476
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 2 cut(s) 399, 604
PciI ACATGT 2 cut(s) 395, 600
PfeI GAWTC 2 cut(s) 113, 318
PkrI GCNGC 1 cut(s) 403
PscI ACATGT 2 cut(s) 395, 600
PspN4I GGNNCC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 6 cut(s) 125, 137, 324, 345, 392, 486
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 2 cut(s) 244, 434
SetI ASST 9 cut(s) 28, 47, 193, 300, 382, 478, 483, 499, 538
SfaNI GCATC 2 cut(s) 121, 601
SmiMI CAYNNNNRTG 1 cut(s) 572
Sse9I AATT 6 cut(s) 143, 181, 224, 519, 619, 627
SsiI CCGC 1 cut(s) 201
TaiI ACGT 2 cut(s) 300, 483
TaqI TCGA 2 cut(s) 433, 540
TasI AATT 6 cut(s) 143, 181, 224, 519, 619, 627
TfiI GAWTC 2 cut(s) 113, 318
Tru1I TTAA 6 cut(s) 125, 137, 324, 345, 392, 486
Tru9I TTAA 6 cut(s) 125, 137, 324, 345, 392, 486
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 7 cut(s) 456, 474, 486, 507, 521, 531, 620
XapI RAATTY 4 cut(s) 143, 224, 619, 627
XceI RCATGY 2 cut(s) 399, 604
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.