Rw7G036060

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000697
Physical Location & Seq
Forward (+)
54254317 .. 54255793
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036060.1

Sequence Viewer

Length: 858 bp
ATGTCTGAAATGGACATGTATGGAGCTGACGAAGTGGAAGAGCAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAATACAAGCAGTGATTTATGTGTTATACGACCTTGTGTTTAGCATACGTGGTGAACATATTAGACGTCCGCTGACTCGTAGACCAGTGACATCAAGTGGATACATATATATGCACAACATATTGGACAGAGACCCTCAAATCTTTAGAGAGGTGTATAGAATGTATCCTGACGTGTTTCGAAAATTATCTCGGCTAATATTTGAGCGATCTCATTTTACTGCTTGCAGAAGTTTCAACAAAGTCTTGAAGGCCTTGAATACAATAGCACCACAGTTTATGGCTAAACCTGAGTCCATACCACCTAACATAAGAGAAAGTACAAGGTTCTATCCTTACTTTAAGGATTGTGTCAGAGCTATAGATGGCACGTATATTCCAGCAACGGTAGTTGGACGTGAGGTAAGCAGATATCGAAATCGCCATGGGAAGATATCACAAAACGTATTAGCAGCTTGTAACTTTGATTTACAGTTCATATATGTGATTAGTGGATGGGAGGGTTCCGCTCATGATTCAAAAGTGTTGAATGATGCGATTTCTAGACGAAATGGACTCAAAGAATGTCGTTCAAATGAATTTCCTCCTAAACCAGAAGAAGATCCGATAGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCAGCAAAGAGAGAATGCTAATGAATGGAGAATGAGTATTGCTACTCATATGTGGACAGATGCCCAACCAAATGCCAACAATGAAAACAATGACAATCAAGAAAGTGAAAATGAAGGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

285

Amino Acids

33.47

Weight (kDa)

5.23

Isoelectric Point (pI)

57.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 146 - 203 2.9e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 157
AciI CCGC 2 cut(s) 146, 582
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 1 cut(s) 653
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 397
AflIII ACRYGT 2 cut(s) 15, 249
AgsI TTSAA 8 cut(s) 313, 325, 334, 594, 604, 648, 707, 722
AjiI CACGTC 2 cut(s) 250, 473
AluBI AGCT 3 cut(s) 26, 434, 530
AluI AGCT 3 cut(s) 26, 434, 530
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 671
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 1 cut(s) 653
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 256
BbvI GCAGC 1 cut(s) 539
BccI CCATC 2 cut(s) 434, 564
BciVI GTATCC 2 cut(s) 170, 252
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 1 cut(s) 435
BfuI GTATCC 2 cut(s) 170, 252
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
BmgBI CACGTC 2 cut(s) 250, 473
BmiI GGNNCC 1 cut(s) 580
BmsI GCATC 2 cut(s) 598, 781
Bpu14I TTCGAA 1 cut(s) 256
BsaAI YACGTR 2 cut(s) 125, 447
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 499
BsaXI ACNNNNNCTCC 2 cut(s) 751, 781
Bse1I ACTGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 2 cut(s) 575, 718
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 539
BshFI GGCC 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 3 cut(s) 284, 676, 730
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 2 cut(s) 146, 582
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 1 cut(s) 358
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 1 cut(s) 580
BspPI GGATC 1 cut(s) 671
BspQI GCTCTTC 1 cut(s) 33
BspT104I TTCGAA 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 584
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 3 cut(s) 284, 676, 730
BssNI GRCGYC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 499
Bst4CI ACNGT 3 cut(s) 351, 463, 549
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 142
BstBAI YACGTR 2 cut(s) 125, 447
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 2 cut(s) 575, 718
BstKTI GATC 3 cut(s) 287, 679, 733
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 3 cut(s) 284, 676, 730
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BsuI GTATCC 2 cut(s) 170, 252
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 499
BtrI CACGTC 2 cut(s) 250, 473
BtsCI GGATG 2 cut(s) 575, 718
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 168
Cac8I GCNNGC 1 cut(s) 301
CciI TCATGA 1 cut(s) 586
Csp6I GTAC 1 cut(s) 396
CviAII CATG 3 cut(s) 16, 500, 587
CviJI RGCY 7 cut(s) 26, 56, 271, 329, 359, 434, 530
CviKI_1 RGCY 7 cut(s) 26, 56, 271, 329, 359, 434, 530
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 1 cut(s) 366
DpnI GATC 3 cut(s) 286, 678, 732
DpnII GATC 3 cut(s) 284, 676, 730
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 499
Eco147I AGGCCT 1 cut(s) 329
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 2 cut(s) 488, 510
EcoRV GATATC 2 cut(s) 488, 510
EcoT14I CCWWGG 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 3 cut(s) 19, 503, 590
FatI CATG 3 cut(s) 15, 499, 586
FauNDI CATATG 1 cut(s) 780
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 2 cut(s) 582, 725
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 1 cut(s) 618
GluI GCNGC 1 cut(s) 528
HaeIII GGCC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 3 cut(s) 19, 503, 590
HinfI GANTC 4 cut(s) 151, 368, 590, 630
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 3 cut(s) 131, 158, 786
Hpy188I TCNGA 3 cut(s) 7, 431, 681
Hpy188III TCNNGA 6 cut(s) 245, 322, 587, 618, 692, 830
Hpy8I GTNNAC 3 cut(s) 131, 158, 786
HpyAV CCTTC 2 cut(s) 319, 839
HpyCH4III ACNGT 3 cut(s) 351, 463, 549
HpyCH4IV ACGT 6 cut(s) 124, 142, 249, 446, 472, 519
HpyCH4V TGCA 2 cut(s) 190, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 6 cut(s) 124, 142, 249, 446, 472, 519
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 3 cut(s) 19, 503, 590
Kzo9I GATC 3 cut(s) 284, 676, 730
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 5 cut(s) 174, 258, 378, 468, 681
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 2 cut(s) 598, 781
MaeI CTAG 1 cut(s) 618
MaeII ACGT 6 cut(s) 124, 142, 249, 446, 472, 519
MaeIII GTNAC 2 cut(s) 163, 533
MalI GATC 3 cut(s) 286, 678, 732
MbiI CCGCTC 1 cut(s) 584
MboI GATC 3 cut(s) 284, 676, 730
MboII GAAGA 5 cut(s) 50, 517, 683, 686, 707
MflI RGATCY 1 cut(s) 676
MluCI AATT 3 cut(s) 260, 653, 700
MlyI GAGTC 3 cut(s) 145, 377, 624
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 6 cut(s) 46, 220, 222, 469, 568, 669
MseI TTAA 3 cut(s) 60, 71, 417
MslI CAYNNNNRTG 2 cut(s) 185, 557
MspA1I CMGCKG 1 cut(s) 148
Mva1269I GAATGC 1 cut(s) 751
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 499
NdeI CATATG 1 cut(s) 780
NdeII GATC 3 cut(s) 284, 676, 730
NlaIII CATG 3 cut(s) 19, 503, 590
NlaIV GGNNCC 1 cut(s) 580
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 256
PagI TCATGA 1 cut(s) 586
PceI AGGCCT 1 cut(s) 329
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 33
PctI GAATGC 1 cut(s) 751
PfeI GAWTC 1 cut(s) 590
PkrI GCNGC 1 cut(s) 529
PleI GAGTC 3 cut(s) 145, 376, 624
PpsI GAGTC 3 cut(s) 145, 376, 624
Ppu21I YACGTR 2 cut(s) 125, 447
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 580
PsuI RGATCY 1 cut(s) 676
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 2 cut(s) 185, 557
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 60, 71, 417
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 3 cut(s) 284, 676, 730
SchI GAGTC 3 cut(s) 145, 377, 624
SfaNI GCATC 2 cut(s) 598, 781
SfcI CTRYAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 185, 557
Sse9I AATT 3 cut(s) 260, 653, 700
SseBI AGGCCT 1 cut(s) 329
SsiI CCGC 2 cut(s) 146, 582
SspI AATATT 1 cut(s) 276
SspMI CTAG 1 cut(s) 618
StuI AGGCCT 1 cut(s) 329
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 3 cut(s) 351, 463, 549
TaiI ACGT 6 cut(s) 127, 145, 252, 449, 475, 522
TaqI TCGA 2 cut(s) 256, 490
TasI AATT 3 cut(s) 260, 653, 700
TatI WGTACW 1 cut(s) 395
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 3 cut(s) 60, 71, 417
Tru9I TTAA 3 cut(s) 60, 71, 417
TscAI CASTG 2 cut(s) 93, 168
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 541, 666, 768, 828, 858
TspRI CASTG 2 cut(s) 93, 168
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 653
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 1 cut(s) 618
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.