Rroxscaffold_1G00010110

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000697
Physical Location & Seq
Forward (+)
12618167 .. 12642827
24661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00010110.1

Sequence Viewer

Length: 387 bp
ATGTCCGAAATGGACATGTATGGAGTTGATGAAGAGGAAGAACAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAACACAAGCAGTGGTTTATGTGTTATACGACCTTGTATTCAGCATACGTGGTGAACGCATTAGACGTCCACCGACTCGGCGACCGTCATTGAGCAAGGATTTCATGATTCAGAAATCGAATCAAACTCTACATCACATAGCGCGCCGCCGTGCAAGGGAGGTTCGGGAGCTTCGGAAGGTCAAGGCGAACCCGTGGGTGAAGTTTGAGAAGGATTTGGGTGAGTTTGTGTTACATAATACTTTAGAAGAACCGGAGATTTGCATACATGAAACTCCTAGCCGATGTACAGTAAATACGTGTGGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

128

Amino Acids

15.15

Weight (kDa)

7.75

Isoelectric Point (pI)

65.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccII CGCG 1 cut(s) 220
AciI CCGC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 379
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 2 cut(s) 15, 374
AluBI AGCT 1 cut(s) 247
AluI AGCT 1 cut(s) 247
AoxI GGCC 1 cut(s) 379
AseI ATTAAT 1 cut(s) 71
AspLEI GCGC 2 cut(s) 220, 222
AsuHPI GGTGA 3 cut(s) 140, 286, 308
BceAI ACGGC 1 cut(s) 210
BfaI CTAG 1 cut(s) 354
BisI GCNGC 1 cut(s) 223
BlsI GCNGC 1 cut(s) 224
BsaAI YACGTR 2 cut(s) 125, 375
BsaHI GRCGYC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 269
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 269
BseLI CCNNNNNNNGG 1 cut(s) 232
BsePI GCGCGC 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 381
BsiEI CGRYCG 1 cut(s) 161
BsiSI CCGG 2 cut(s) 329, 382
BslI CCNNNNNNNGG 1 cut(s) 232
BsnI GGCC 1 cut(s) 381
Bsp1407I TGTACA 1 cut(s) 362
BspACI CCGC 1 cut(s) 223
BspANI GGCC 1 cut(s) 381
BspFNI CGCG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 381
BsrGI TGTACA 1 cut(s) 362
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 1 cut(s) 269
BssHII GCGCGC 1 cut(s) 218
BssNI GRCGYC 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 45, 162, 367
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 142
BstAUI TGTACA 1 cut(s) 362
BstBAI YACGTR 2 cut(s) 125, 375
BstC8I GCNNGC 1 cut(s) 220
BstDSI CCRYGG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 220
BstHHI GCGC 2 cut(s) 220, 222
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstUI CGCG 1 cut(s) 220
BsuRI GGCC 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 220
CciI TCATGA 1 cut(s) 180
CfoI GCGC 2 cut(s) 220, 222
Cfr10I RCCGGY 1 cut(s) 381
Csp6I GTAC 1 cut(s) 363
CviAII CATG 3 cut(s) 16, 181, 344
CviJI RGCY 4 cut(s) 56, 247, 357, 381
CviKI_1 RGCY 4 cut(s) 56, 247, 357, 381
CviQI GTAC 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
FaeI CATG 3 cut(s) 19, 184, 347
FatI CATG 3 cut(s) 15, 180, 343
Fnu4HI GCNGC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 223
FspBI CTAG 1 cut(s) 354
GlaI GCGC 2 cut(s) 219, 221
GluI GCNGC 1 cut(s) 223
HaeIII GGCC 1 cut(s) 381
HapII CCGG 2 cut(s) 329, 382
HhaI GCGC 2 cut(s) 220, 222
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 3 cut(s) 19, 184, 347
Hin6I GCGC 2 cut(s) 218, 220
HinP1I GCGC 2 cut(s) 218, 220
HinfI GANTC 3 cut(s) 151, 184, 196
HpaII CCGG 2 cut(s) 329, 382
HphI GGTGA 3 cut(s) 140, 286, 308
Hpy166II GTNNAC 2 cut(s) 131, 146
Hpy188I TCNGA 3 cut(s) 7, 189, 252
Hpy188III TCNNGA 2 cut(s) 181, 242
Hpy8I GTNNAC 2 cut(s) 131, 146
HpyAV CCTTC 2 cut(s) 247, 280
HpyCH4III ACNGT 3 cut(s) 45, 162, 367
HpyCH4IV ACGT 3 cut(s) 124, 142, 374
HpyCH4V TGCA 2 cut(s) 230, 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpySE526I ACGT 3 cut(s) 124, 142, 374
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 3 cut(s) 19, 184, 347
HspAI GCGC 2 cut(s) 218, 220
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 1 cut(s) 342
MaeI CTAG 1 cut(s) 354
MaeII ACGT 3 cut(s) 124, 142, 374
MaeIII GTNAC 1 cut(s) 306
MboII GAAGA 3 cut(s) 44, 50, 335
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 3 cut(s) 28, 46, 229
MseI TTAA 2 cut(s) 60, 71
MspI CCGG 2 cut(s) 329, 382
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 83
NlaIII CATG 3 cut(s) 19, 184, 347
NmeAIII GCCGAG 1 cut(s) 133
NspI RCATGY 1 cut(s) 19
PagI TCATGA 1 cut(s) 180
PauI GCGCGC 1 cut(s) 218
PciI ACATGT 1 cut(s) 15
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PfeI GAWTC 2 cut(s) 184, 196
PkrI GCNGC 1 cut(s) 224
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 2 cut(s) 125, 375
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PteI GCGCGC 1 cut(s) 218
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 60, 71
SatI GCNGC 1 cut(s) 223
SchI GAGTC 1 cut(s) 145
SetI ASST 7 cut(s) 111, 127, 145, 240, 249, 258, 377
SsiI CCGC 1 cut(s) 223
SspMI CTAG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 45, 162, 367
TaiI ACGT 3 cut(s) 127, 145, 377
TaqI TCGA 1 cut(s) 194
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 2 cut(s) 184, 196
Tru1I TTAA 2 cut(s) 60, 71
Tru9I TTAA 2 cut(s) 60, 71
TscAI CASTG 1 cut(s) 93
TspDTI ATGAA 3 cut(s) 45, 169, 360
TspRI CASTG 1 cut(s) 93
VspI ATTAAT 1 cut(s) 71
XceI RCATGY 1 cut(s) 19
XspI CTAG 1 cut(s) 354
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.