nuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 509 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 530 |
| AccB1I | GGYRCC | 1 cut(s) | 263 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 589 |
| AciI | CCGC | 1 cut(s) | 868 |
| AcoI | YGGCCR | 1 cut(s) | 610 |
| AcsI | RAATTY | 3 cut(s) | 116, 229, 631 |
| AcuI | CTGAAG | 2 cut(s) | 38, 79 |
| AcyI | GRCGYC | 1 cut(s) | 264 |
| AfaI | GTAC | 1 cut(s) | 428 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 224, 589, 773, 795 |
| AflII | CTTAAG | 1 cut(s) | 316 |
| AgsI | TTSAA | 7 cut(s) | 47, 63, 121, 235, 300, 361, 691 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 215, 247 |
| AleI | CACNNNNGTG | 1 cut(s) | 665 |
| AluBI | AGCT | 8 cut(s) | 16, 22, 113, 321, 505, 702, 735, 890 |
| AluI | AGCT | 8 cut(s) | 16, 22, 113, 321, 505, 702, 735, 890 |
| Alw21I | GWGCWC | 1 cut(s) | 462 |
| Alw26I | GTCTC | 1 cut(s) | 675 |
| AoxI | GGCC | 2 cut(s) | 591, 610 |
| ApeKI | GCWGC | 1 cut(s) | 71 |
| ApoI | RAATTY | 3 cut(s) | 116, 229, 631 |
| AspLEI | GCGC | 1 cut(s) | 266 |
| AsuHPI | GGTGA | 1 cut(s) | 207 |
| AxyI | CCTNAGG | 1 cut(s) | 205 |
| BanI | GGYRCC | 1 cut(s) | 263 |
| Bbv12I | GWGCWC | 1 cut(s) | 462 |
| BbvI | GCAGC | 1 cut(s) | 83 |
| BccI | CCATC | 4 cut(s) | 85, 197, 431, 740 |
| BceAI | ACGGC | 1 cut(s) | 625 |
| BclI | TGATCA | 2 cut(s) | 27, 652 |
| BcoDI | GTCTC | 1 cut(s) | 675 |
| BfaI | CTAG | 1 cut(s) | 434 |
| BfoI | RGCGCY | 1 cut(s) | 267 |
| BfrI | CTTAAG | 1 cut(s) | 316 |
| BisI | GCNGC | 1 cut(s) | 72 |
| BlsI | GCNGC | 1 cut(s) | 73 |
| BmiI | GGNNCC | 1 cut(s) | 265 |
| BmsI | GCATC | 1 cut(s) | 504 |
| Bpu10I | CCTNAGC | 1 cut(s) | 322 |
| BpuEI | CTTGAG | 3 cut(s) | 255, 503, 785 |
| Bsa29I | ATCGAT | 1 cut(s) | 681 |
| BsaHI | GRCGYC | 1 cut(s) | 264 |
| BsaJI | CCNNGG | 1 cut(s) | 259 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 224, 589, 773, 795 |
| Bse1I | ACTGG | 2 cut(s) | 613, 637 |
| Bse21I | CCTNAGG | 1 cut(s) | 205 |
| Bse3DI | GCAATG | 1 cut(s) | 549 |
| BseCI | ATCGAT | 1 cut(s) | 681 |
| BseDI | CCNNGG | 1 cut(s) | 259 |
| BseGI | GGATG | 1 cut(s) | 417 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 224, 589, 773, 795 |
| BseMI | GCAATG | 1 cut(s) | 549 |
| BseMII | CTCAG | 4 cut(s) | 99, 219, 384, 686 |
| BseNI | ACTGG | 2 cut(s) | 613, 637 |
| BseXI | GCAGC | 1 cut(s) | 83 |
| BshFI | GGCC | 2 cut(s) | 593, 612 |
| BshNI | GGYRCC | 1 cut(s) | 263 |
| BshVI | ATCGAT | 1 cut(s) | 681 |
| BsiHKAI | GWGCWC | 1 cut(s) | 462 |
| BslFI | GGGAC | 4 cut(s) | 128, 260, 344, 748 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 224, 589, 773, 795 |
| BsmAI | GTCTC | 1 cut(s) | 675 |
| BsmFI | GGGAC | 4 cut(s) | 128, 260, 344, 748 |
| BsmI | GAATGC | 1 cut(s) | 295 |
| BsnI | GGCC | 2 cut(s) | 593, 612 |
| Bsp1286I | GDGCHC | 1 cut(s) | 462 |
| Bsp143I | GATC | 2 cut(s) | 27, 652 |
| BspACI | CCGC | 1 cut(s) | 868 |
| BspANI | GGCC | 2 cut(s) | 593, 612 |
| BspCNI | CTCAG | 4 cut(s) | 98, 218, 383, 685 |
| BspDI | ATCGAT | 1 cut(s) | 681 |
| BspLI | GGNNCC | 1 cut(s) | 265 |
| BspT107I | GGYRCC | 1 cut(s) | 263 |
| BspTI | CTTAAG | 1 cut(s) | 316 |
| BsrDI | GCAATG | 1 cut(s) | 549 |
| BsrI | ACTGG | 2 cut(s) | 613, 637 |
| BssECI | CCNNGG | 1 cut(s) | 259 |
| BssMI | GATC | 2 cut(s) | 27, 652 |
| BssNI | GRCGYC | 1 cut(s) | 264 |
| Bst4CI | ACNGT | 2 cut(s) | 335, 667 |
| BstACI | GRCGYC | 1 cut(s) | 264 |
| BstAFI | CTTAAG | 1 cut(s) | 316 |
| BstC8I | GCNNGC | 2 cut(s) | 733, 790 |
| BstDEI | CTNAG | 6 cut(s) | 85, 205, 322, 370, 672, 727 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 793 |
| BstF5I | GGATG | 1 cut(s) | 417 |
| BstH2I | RGCGCY | 1 cut(s) | 267 |
| BstHHI | GCGC | 1 cut(s) | 266 |
| BstKTI | GATC | 2 cut(s) | 30, 655 |
| BstMAI | GTCTC | 1 cut(s) | 675 |
| BstMBI | GATC | 2 cut(s) | 27, 652 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 551, 896 |
| BstV1I | GCAGC | 1 cut(s) | 83 |
| Bsu15I | ATCGAT | 1 cut(s) | 681 |
| Bsu36I | CCTNAGG | 1 cut(s) | 205 |
| BsuRI | GGCC | 2 cut(s) | 593, 612 |
| BsuTUI | ATCGAT | 1 cut(s) | 681 |
| BtsCI | GGATG | 1 cut(s) | 417 |
| BtsI | GCAGTG | 1 cut(s) | 783 |
| BtsIMutI | CAGTG | 4 cut(s) | 249, 340, 672, 783 |
| Cac8I | GCNNGC | 2 cut(s) | 733, 790 |
| CfoI | GCGC | 1 cut(s) | 266 |
| ClaI | ATCGAT | 1 cut(s) | 681 |
| Csp6I | GTAC | 1 cut(s) | 427 |
| CviAII | CATG | 2 cut(s) | 381, 821 |
| CviQI | GTAC | 1 cut(s) | 427 |
| DdeI | CTNAG | 6 cut(s) | 85, 205, 322, 370, 672, 727 |
| DinI | GGCGCC | 1 cut(s) | 265 |
| DpnI | GATC | 2 cut(s) | 29, 654 |
| DpnII | GATC | 2 cut(s) | 27, 652 |
| DraI | TTTAAA | 1 cut(s) | 466 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 530 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 530 |
| EaeI | YGGCCR | 1 cut(s) | 610 |
| Eco32I | GATATC | 1 cut(s) | 91 |
| Eco57I | CTGAAG | 2 cut(s) | 38, 79 |
| Eco81I | CCTNAGG | 1 cut(s) | 205 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 793 |
| EcoRI | GAATTC | 1 cut(s) | 116 |
| EcoRV | GATATC | 1 cut(s) | 91 |
| EgeI | GGCGCC | 1 cut(s) | 265 |
| EheI | GGCGCC | 1 cut(s) | 265 |
| FaeI | CATG | 2 cut(s) | 384, 824 |
| FaqI | GGGAC | 4 cut(s) | 128, 260, 344, 748 |
| FatI | CATG | 2 cut(s) | 380, 820 |
| FbaI | TGATCA | 2 cut(s) | 27, 652 |
| Fnu4HI | GCNGC | 1 cut(s) | 72 |
| FokI | GGATG | 1 cut(s) | 404 |
| Fsp4HI | GCNGC | 1 cut(s) | 72 |
| FspBI | CTAG | 1 cut(s) | 434 |
| GlaI | GCGC | 1 cut(s) | 265 |
| GluI | GCNGC | 1 cut(s) | 72 |
| HaeII | RGCGCY | 1 cut(s) | 267 |
| HaeIII | GGCC | 2 cut(s) | 593, 612 |
| HhaI | GCGC | 1 cut(s) | 266 |
| Hin1I | GRCGYC | 1 cut(s) | 264 |
| Hin1II | CATG | 2 cut(s) | 384, 824 |
| Hin6I | GCGC | 1 cut(s) | 264 |
| HinP1I | GCGC | 1 cut(s) | 264 |
| HindIII | AAGCTT | 3 cut(s) | 20, 319, 503 |
| HinfI | GANTC | 3 cut(s) | 377, 694, 848 |
| HphI | GGTGA | 1 cut(s) | 207 |
| Hpy166II | GTNNAC | 1 cut(s) | 809 |
| Hpy188I | TCNGA | 8 cut(s) | 88, 98, 139, 280, 472, 490, 500, 579 |
| Hpy188III | TCNNGA | 4 cut(s) | 286, 434, 691, 802 |
| Hpy8I | GTNNAC | 1 cut(s) | 809 |
| HpyAV | CCTTC | 4 cut(s) | 185, 375, 715, 827 |
| HpyCH4III | ACNGT | 2 cut(s) | 335, 667 |
| HpyCH4IV | ACGT | 2 cut(s) | 237, 573 |
| HpyCH4V | TGCA | 1 cut(s) | 495 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 551, 896 |
| HpyF3I | CTNAG | 6 cut(s) | 85, 205, 322, 370, 672, 727 |
| HpySE526I | ACGT | 2 cut(s) | 237, 573 |
| Hsp92I | GRCGYC | 1 cut(s) | 264 |
| Hsp92II | CATG | 2 cut(s) | 384, 824 |
| HspAI | GCGC | 1 cut(s) | 264 |
| KasI | GGCGCC | 1 cut(s) | 263 |
| Ksp22I | TGATCA | 2 cut(s) | 27, 652 |
| Kzo9I | GATC | 2 cut(s) | 27, 652 |
| LmnI | GCTCC | 2 cut(s) | 110, 559 |
| LpnPI | CCDG | 6 cut(s) | 192, 434, 626, 650, 780, 802 |
| Lsp1109I | GCAGC | 1 cut(s) | 83 |
| LweI | GCATC | 1 cut(s) | 504 |
| MaeI | CTAG | 1 cut(s) | 434 |
| MaeII | ACGT | 2 cut(s) | 237, 573 |
| MaeIII | GTNAC | 1 cut(s) | 353 |
| MalI | GATC | 2 cut(s) | 29, 654 |
| MboI | GATC | 2 cut(s) | 27, 652 |
| MboII | GAAGA | 5 cut(s) | 85, 204, 393, 613, 772 |
| MhlI | GDGCHC | 1 cut(s) | 462 |
| MluCI | AATT | 4 cut(s) | 116, 229, 631, 900 |
| Mly113I | GGCGCC | 1 cut(s) | 264 |
| MlyI | GAGTC | 1 cut(s) | 688 |
| MnlI | CCTC | 7 cut(s) | 62, 214, 269, 277, 320, 379, 527 |
| MseI | TTAA | 3 cut(s) | 150, 317, 465 |
| MslI | CAYNNNNRTG | 2 cut(s) | 300, 665 |
| MspCI | CTTAAG | 1 cut(s) | 316 |
| Mva1269I | GAATGC | 1 cut(s) | 295 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 551, 896 |
| NarI | GGCGCC | 1 cut(s) | 264 |
| NdeII | GATC | 2 cut(s) | 27, 652 |
| NlaIII | CATG | 2 cut(s) | 384, 824 |
| NlaIV | GGNNCC | 1 cut(s) | 265 |
| NmeAIII | GCCGAG | 1 cut(s) | 240 |
| OliI | CACNNNNGTG | 1 cut(s) | 665 |
| PctI | GAATGC | 1 cut(s) | 295 |
| PfeI | GAWTC | 2 cut(s) | 377, 848 |
| PflFI | GACNNNGTC | 1 cut(s) | 335 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 589 |
| PkrI | GCNGC | 1 cut(s) | 73 |
| PleI | GAGTC | 1 cut(s) | 688 |
| PluTI | GGCGCC | 1 cut(s) | 267 |
| PpsI | GAGTC | 1 cut(s) | 688 |
| PsiI | TTATAA | 1 cut(s) | 509 |
| PspN4I | GGNNCC | 1 cut(s) | 265 |
| PsyI | GACNNNGTC | 1 cut(s) | 335 |
| RsaI | GTAC | 1 cut(s) | 428 |
| RsaNI | GTAC | 1 cut(s) | 427 |
| RseI | CAYNNNNRTG | 2 cut(s) | 300, 665 |
| SaqAI | TTAA | 3 cut(s) | 150, 317, 465 |
| SatI | GCNGC | 1 cut(s) | 72 |
| Sau3AI | GATC | 2 cut(s) | 27, 652 |
| SchI | GAGTC | 1 cut(s) | 688 |
| SduI | GDGCHC | 1 cut(s) | 462 |
| SfaNI | GCATC | 1 cut(s) | 504 |
| SfoI | GGCGCC | 1 cut(s) | 265 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 300, 665 |
| SmlI | CTYRAG | 4 cut(s) | 270, 316, 518, 800 |
| SmoI | CTYRAG | 4 cut(s) | 270, 316, 518, 800 |
| Sse9I | AATT | 4 cut(s) | 116, 229, 631, 900 |
| SsiI | CCGC | 1 cut(s) | 868 |
| SspDI | GGCGCC | 1 cut(s) | 263 |
| SspMI | CTAG | 1 cut(s) | 434 |
| TaaI | ACNGT | 2 cut(s) | 335, 667 |
| TaiI | ACGT | 2 cut(s) | 240, 576 |
| TaqI | TCGA | 2 cut(s) | 192, 681 |
| TasI | AATT | 4 cut(s) | 116, 229, 631, 900 |
| TatI | WGTACW | 1 cut(s) | 426 |
| TfiI | GAWTC | 2 cut(s) | 377, 848 |
| Tru1I | TTAA | 3 cut(s) | 150, 317, 465 |
| Tru9I | TTAA | 3 cut(s) | 150, 317, 465 |
| TscAI | CASTG | 4 cut(s) | 256, 340, 672, 790 |
| TseI | GCWGC | 1 cut(s) | 71 |
| TspDTI | ATGAA | 4 cut(s) | 31, 369, 809, 888 |
| TspRI | CASTG | 4 cut(s) | 256, 340, 672, 790 |
| Tth111I | GACNNNGTC | 1 cut(s) | 335 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 589 |
| Vha464I | CTTAAG | 1 cut(s) | 316 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 793 |
| XapI | RAATTY | 3 cut(s) | 116, 229, 631 |
| XbaI | TCTAGA | 1 cut(s) | 433 |
| XspI | CTAG | 1 cut(s) | 434 |