FvH4_6g24701

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000697
Physical Location & Seq
Forward (+)
18722061 .. 18723159
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24701.t1

Sequence Viewer

Length: 378 bp
ATGATATTCGAGCGATCCCATTTCACTGTTAGCAGAAGTTTCAACAAAATCTTGAAGGCCTTAAATACGATAGCTCCAGAGTTTATGGCTAAACCACCGCCACCTGATACCACACCACCAAACATAAGAGAAAGATTGCATAATTTTCTTCGACAAGAATGCCGTTCAGATGAATTTCCTCCTGAACCAGAAGATGATCCGGTAGAGAATGAAGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGAGCAGCAAAGAGATAATGCTAATCAATGGAGAATGAGTATTGCTAATCAAATGTGGGCAGAAGCTCAAGCAAATGCCAACAATGTAAACAATGACAATGAAGAAAGTGATCATGAAGGAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

126

Amino Acids

14.75

Weight (kDa)

4.35

Isoelectric Point (pI)

65.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 98
AclWI GGATC 2 cut(s) 9, 191
AcsI RAATTY 1 cut(s) 173
AgsI TTSAA 4 cut(s) 43, 55, 227, 242
AluBI AGCT 2 cut(s) 74, 314
AluI AGCT 2 cut(s) 74, 314
AlwI GGATC 2 cut(s) 9, 191
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 173
BbvI GCAGC 1 cut(s) 265
BceAI ACGGC 1 cut(s) 147
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BclI TGATCA 1 cut(s) 358
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 271, 301
BseGI GGATG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 200
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 1 cut(s) 59
Bsp143I GATC 3 cut(s) 14, 196, 358
BspACI CCGC 1 cut(s) 98
BspANI GGCC 1 cut(s) 59
BspHI TCATGA 1 cut(s) 361
BspPI GGATC 2 cut(s) 9, 191
BssMI GATC 3 cut(s) 14, 196, 358
Bst4CI ACNGT 1 cut(s) 28
BstF5I GGATG 1 cut(s) 238
BstKTI GATC 3 cut(s) 17, 199, 361
BstMBI GATC 3 cut(s) 14, 196, 358
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 59
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 24
CciI TCATGA 1 cut(s) 361
CviAII CATG 1 cut(s) 362
CviJI RGCY 4 cut(s) 59, 74, 89, 314
CviKI_1 RGCY 4 cut(s) 59, 74, 89, 314
DpnI GATC 3 cut(s) 16, 198, 360
DpnII GATC 3 cut(s) 14, 196, 358
Eco147I AGGCCT 1 cut(s) 59
FaeI CATG 1 cut(s) 365
FaiI YATR 4 cut(s) 86, 125, 141, 363
FatI CATG 1 cut(s) 361
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 200
Hin1II CATG 1 cut(s) 365
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 52, 77, 182, 362
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 2 cut(s) 49, 359
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4V TGCA 1 cut(s) 139
Hsp92II CATG 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 14, 196, 358
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 5 cut(s) 90, 117, 195, 201, 213
Lsp1109I GCAGC 1 cut(s) 265
MalI GATC 3 cut(s) 16, 198, 360
MboI GATC 3 cut(s) 14, 196, 358
MboII GAAGA 5 cut(s) 140, 203, 224, 227, 362
MluCI AATT 3 cut(s) 142, 173, 220
MnlI CCTC 1 cut(s) 189
MseI TTAA 1 cut(s) 62
MspI CCGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 164
NdeII GATC 3 cut(s) 14, 196, 358
NlaIII CATG 1 cut(s) 365
PagI TCATGA 1 cut(s) 361
PceI AGGCCT 1 cut(s) 59
PctI GAATGC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 3 cut(s) 14, 196, 358
SetI ASST 3 cut(s) 76, 106, 316
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 3 cut(s) 142, 173, 220
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 59
TaaI ACNGT 1 cut(s) 28
TaqI TCGA 2 cut(s) 9, 151
TasI AATT 3 cut(s) 142, 173, 220
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 31
TseI GCWGC 1 cut(s) 253
TspDTI ATGAA 4 cut(s) 186, 225, 363, 378
TspRI CASTG 1 cut(s) 31
XapI RAATTY 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.