Rmu_sc0006833.1_g000005

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006833.1
OG0000697
Physical Location & Seq
Reverse (-)
24336 .. 25273
938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006833.1_g000005.1.cds

Sequence Viewer

Length: 780 bp
atggtaaaaggtcgcgaagtaagcagttatcgtaatcgtcatggaattcagtctcaaaatgttttggcagcttgcaactttgatttgcaattcatatatgtgctaagtgggtgggaaggttcggcacatgattcaaaattgttaaatgatgccttattaaggagaaatggacttgaagttcctcaagtggattgtggatttgctaatcgacgccaattcttagctccgttacgaagtgttcgatatcatctcaaagattttggtggtcaaggtcgccatcctcgaaatgcaagtgacttgttcaatcttcgccatgcgtcattgaggaatgtgattgagagaatatttggtatctttaaatcacggttcacaattttcaaaaccgcacctccattcccatatcaaacacaagcagaattggtgttagcttgtgctggactacacaactttcttcgcaaagaatgtcgtgaggatgaatttcctgttgaaccagaaaacgattcatcatctccatcctatctagatatggaagatgagaatcttgaactactttctcaaagtcaacaacgacaaagagcggaggctaatgcttggaggcttactattgctgaagctatgtgggaggatagaccgcgaaatgatgatgataatggaagtcaagagaataacaatgatactcaagacaatgagaatgagaataatgaggaacatatggatgacggggaacaagaaggttatgatgataatgaagttggaatggatgagtatgcagctttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

259

Amino Acids

29.85

Weight (kDa)

4.77

Isoelectric Point (pI)

55.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 578
AccII CGCG 2 cut(s) 15, 634
AciI CCGC 3 cut(s) 384, 578, 632
AcsI RAATTY 2 cut(s) 45, 476
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 211
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 6 cut(s) 135, 176, 304, 379, 488, 545
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 5 cut(s) 71, 224, 428, 614, 773
AluI AGCT 5 cut(s) 71, 224, 428, 614, 773
Alw26I GTCTC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 68, 770
ApoI RAATTY 2 cut(s) 45, 476
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 285, 520
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 521
BisI GCNGC 2 cut(s) 69, 771
BlsI GCNGC 2 cut(s) 70, 772
BmsI GCATC 1 cut(s) 139
BpuEI CTTGAG 2 cut(s) 168, 663
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse8I GATNNNNATC 1 cut(s) 537
BseGI GGATG 5 cut(s) 277, 478, 512, 721, 766
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 2 cut(s) 15, 634
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 57
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 3 cut(s) 384, 578, 632
BspFNI CGCG 2 cut(s) 15, 634
BsrBI CCGCTC 1 cut(s) 578
BssNI GRCGYC 1 cut(s) 211
Bst4CI ACNGT 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 104, 220
BstENI CCTNNNNNAGG 1 cut(s) 157
BstF5I GGATG 5 cut(s) 277, 478, 512, 721, 766
BstFNI CGCG 2 cut(s) 15, 634
BstMAI GTCTC 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstUI CGCG 2 cut(s) 15, 634
BstV1I GCAGC 1 cut(s) 80
BtsCI GGATG 5 cut(s) 277, 478, 512, 721, 766
BtuMI TCGCGA 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 73
CseI GACGC 2 cut(s) 219, 306
CviAII CATG 3 cut(s) 41, 128, 314
CviJI RGCY 7 cut(s) 71, 224, 428, 584, 598, 614, 773
CviKI_1 RGCY 7 cut(s) 71, 224, 428, 584, 598, 614, 773
DdeI CTNAG 2 cut(s) 104, 220
DraI TTTAAA 1 cut(s) 358
Eco32I GATATC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 630
EcoNI CCTNNNNNAGG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 45
EcoRV GATATC 1 cut(s) 245
FaeI CATG 3 cut(s) 44, 131, 317
FatI CATG 3 cut(s) 40, 127, 313
FauNDI CATATG 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 69, 771
FokI GGATG 5 cut(s) 264, 485, 499, 728, 773
Fsp4HI GCNGC 2 cut(s) 69, 771
FspBI CTAG 1 cut(s) 521
GluI GCNGC 2 cut(s) 69, 771
HgaI GACGC 2 cut(s) 219, 306
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 3 cut(s) 44, 131, 317
HincII GTYRAC 1 cut(s) 563
HindII GTYRAC 1 cut(s) 563
HinfI GANTC 3 cut(s) 131, 500, 538
Hpy166II GTNNAC 2 cut(s) 369, 563
Hpy188III TCNNGA 6 cut(s) 14, 467, 521, 542, 659, 680
Hpy8I GTNNAC 2 cut(s) 369, 563
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 2 cut(s) 110, 725
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 75, 88, 290, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 2 cut(s) 104, 220
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 3 cut(s) 44, 131, 317
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 3 cut(s) 420, 495, 504
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 1 cut(s) 139
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 2 cut(s) 228, 293
MbiI CCGCTC 1 cut(s) 578
MboII GAAGA 3 cut(s) 299, 443, 542
MluCI AATT 7 cut(s) 45, 89, 137, 215, 372, 416, 476
MmeI TCCRAC 1 cut(s) 733
MnlI CCTC 9 cut(s) 192, 291, 318, 399, 463, 574, 588, 616, 697
MseI TTAA 3 cut(s) 143, 158, 357
MslI CAYNNNNRTG 2 cut(s) 98, 714
MvnI CGCG 2 cut(s) 15, 634
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeI CATATG 1 cut(s) 711
NlaIII CATG 3 cut(s) 44, 131, 317
NmuCI GTSAC 1 cut(s) 293
NruI TCGCGA 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 238, 280
PfeI GAWTC 3 cut(s) 131, 500, 538
PkrI GCNGC 2 cut(s) 70, 772
RruI TCGCGA 1 cut(s) 15
RseI CAYNNNNRTG 2 cut(s) 98, 714
SaqAI TTAA 3 cut(s) 143, 158, 357
SatI GCNGC 2 cut(s) 69, 771
SfaNI GCATC 1 cut(s) 139
SmiMI CAYNNNNRTG 2 cut(s) 98, 714
SmlI CTYRAG 2 cut(s) 183, 678
SmoI CTYRAG 2 cut(s) 183, 678
Sse9I AATT 7 cut(s) 45, 89, 137, 215, 372, 416, 476
SsiI CCGC 3 cut(s) 384, 578, 632
SspI AATATT 1 cut(s) 345
SspMI CTAG 1 cut(s) 521
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 3 cut(s) 208, 241, 283
TasI AATT 7 cut(s) 45, 89, 137, 215, 372, 416, 476
TfiI GAWTC 3 cut(s) 131, 500, 538
Tru1I TTAA 3 cut(s) 143, 158, 357
Tru9I TTAA 3 cut(s) 143, 158, 357
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 68, 770
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 4 cut(s) 82, 489, 492, 762
TspGWI ACGGA 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 157
XapI RAATTY 2 cut(s) 45, 476
XbaI TCTAGA 1 cut(s) 520
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.