Rmu_sc0000548.1_g000007
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000548.1
OG0000697
Physical Location & Seq
Forward (+)
29306 .. 30458
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000548.1_g000007.1.cds

Sequence Viewer

Length: 720 bp
atgcgtaagacattttctaatgagaagggaaagtggcccaatgataaaggaaggttaccagtatgggtaacaggcattgaatcgccattacccattggggacaccattgtgggcctggacccagaagcccagtggcccaaggtgatctggcagcccaattttttggaggagaaaacatatttaggggggtgtattgtatatggaattactgtatatggaaaatcatggacgtctgcttctgatgatcgacgccggtttttagctccatttcgaggtactcgatatcatctcaaagattttggtggtgaaggaaaccatcctgtcaatgcaattgagttattcaatcttcgtcatgcttccttgaggaatgtaattgagagaatatttggaatatttaagtcgcgtttcacaatcttcaaatcagcaccaccattttcatataagacacaagcagagctagttttggcttgtgcaggactgcacaattttcttcgacaggaatgccgttcagatgaatttcctcccgaaccagaagaagatccgatagacaatcacgaagataattttgaatgggatgattttcaaacccaagatcagcagagagaaaatgctaatgaatggagaatgagtattgctactcatatgtggacagatgcccaaccaaatgccaacaatgaaaacaataacaatgaagaaagtgaaaatgaaggagaaggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

239

Amino Acids

27.61

Weight (kDa)

5.01

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 233
AccII CGCG 1 cut(s) 403
AclWI GGATC 1 cut(s) 533
AcsI RAATTY 1 cut(s) 515
AcyI GRCGYC 2 cut(s) 230, 250
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 5 cut(s) 80, 343, 418, 569, 584
AjnI CCWGG 1 cut(s) 114
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 2 cut(s) 263, 457
AluI AGCT 2 cut(s) 263, 457
AlwI GGATC 1 cut(s) 533
AoxI GGCC 3 cut(s) 35, 112, 134
ApeKI GCWGC 1 cut(s) 151
ApoI RAATTY 1 cut(s) 515
AspS9I GGNCC 4 cut(s) 36, 112, 118, 135
AsuHPI GGTGA 2 cut(s) 154, 317
AvaII GGWCC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 163
BccI CCATC 1 cut(s) 324
BceAI ACGGC 1 cut(s) 489
BcgI CGANNNNNNTGC 2 cut(s) 483, 517
BciT130I CCWGG 1 cut(s) 116
BfaI CTAG 1 cut(s) 458
BisI GCNGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 153
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 4 cut(s) 36, 112, 118, 135
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 382
BsaHI GRCGYC 2 cut(s) 230, 250
BsaJI CCNNGG 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 2 cut(s) 59, 130
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 2 cut(s) 316, 580
BseLI CCNNNNNNNGG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 59, 130
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 163
BsgI GTGCAG 2 cut(s) 464, 492
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 3 cut(s) 37, 114, 136
BsiSI CCGG 1 cut(s) 253
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 3 cut(s) 37, 114, 136
Bsp143I GATC 4 cut(s) 144, 244, 538, 592
BspANI GGCC 3 cut(s) 37, 114, 136
BspFNI CGCG 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 120
BspPI GGATC 1 cut(s) 533
BsrFI RCCGGY 1 cut(s) 252
BsrI ACTGG 2 cut(s) 59, 130
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 4 cut(s) 144, 244, 538, 592
BssNI GRCGYC 2 cut(s) 230, 250
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 211
BstACI GRCGYC 2 cut(s) 230, 250
BstEII GGTNACC 1 cut(s) 54
BstF5I GGATG 2 cut(s) 316, 580
BstFNI CGCG 1 cut(s) 403
BstKTI GATC 4 cut(s) 147, 247, 541, 595
BstMBI GATC 4 cut(s) 144, 244, 538, 592
BstNI CCWGG 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 538
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 3 cut(s) 37, 114, 136
BtsCI GGATG 2 cut(s) 316, 580
BtsIMutI CAGTG 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 252
Cfr13I GGNCC 4 cut(s) 36, 112, 118, 135
CseI GACGC 1 cut(s) 258
Csp6I GTAC 1 cut(s) 276
CviAII CATG 2 cut(s) 225, 353
CviJI RGCY 8 cut(s) 37, 114, 128, 136, 154, 263, 457, 467
CviKI_1 RGCY 8 cut(s) 37, 114, 128, 136, 154, 263, 457, 467
CviQI GTAC 1 cut(s) 276
DpnI GATC 4 cut(s) 146, 246, 540, 594
DpnII GATC 4 cut(s) 144, 244, 538, 592
Eco130I CCWWGG 1 cut(s) 138
Eco32I GATATC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 118
Eco91I GGTNACC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 114
EcoRV GATATC 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 228, 356
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 224, 352
FauNDI CATATG 1 cut(s) 642
Fnu4HI GCNGC 1 cut(s) 152
FokI GGATG 2 cut(s) 303, 587
Fsp4HI GCNGC 1 cut(s) 152
FspBI CTAG 1 cut(s) 458
GluI GCNGC 1 cut(s) 152
HaeIII GGCC 3 cut(s) 37, 114, 136
HapII CCGG 1 cut(s) 253
HgaI GACGC 1 cut(s) 258
Hin1I GRCGYC 2 cut(s) 230, 250
Hin1II CATG 2 cut(s) 228, 356
HinfI GANTC 1 cut(s) 80
HpaII CCGG 1 cut(s) 253
HphI GGTGA 2 cut(s) 154, 317
Hpy166II GTNNAC 1 cut(s) 648
Hpy188I TCNGA 3 cut(s) 241, 511, 543
Hpy188III TCNNGA 2 cut(s) 524, 554
Hpy8I GTNNAC 1 cut(s) 648
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 5 cut(s) 19, 45, 302, 701, 707
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 3 cut(s) 329, 473, 481
HpySE526I ACGT 1 cut(s) 230
Hsp92I GRCGYC 2 cut(s) 230, 250
Hsp92II CATG 2 cut(s) 228, 356
Kzo9I GATC 4 cut(s) 144, 244, 538, 592
LmnI GCTCC 1 cut(s) 268
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 643
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 54, 67
MalI GATC 4 cut(s) 146, 246, 540, 594
MboI GATC 4 cut(s) 144, 244, 538, 592
MboII GAAGA 7 cut(s) 338, 406, 482, 545, 548, 569, 704
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 538
MluCI AATT 7 cut(s) 157, 204, 330, 372, 484, 515, 562
MnlI CCTC 4 cut(s) 160, 266, 357, 531
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 1 cut(s) 116
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 506
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 403
NdeI CATATG 1 cut(s) 642
NdeII GATC 4 cut(s) 144, 244, 538, 592
NlaIII CATG 2 cut(s) 228, 356
NlaIV GGNNCC 1 cut(s) 120
OliI CACNNNNGTG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 114
PspEI GGTNACC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 4 cut(s) 36, 112, 118, 135
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 1 cut(s) 152
Sau3AI GATC 4 cut(s) 144, 244, 538, 592
Sau96I GGNCC 4 cut(s) 36, 112, 118, 135
ScrFI CCNGG 1 cut(s) 116
SetI ASST 6 cut(s) 56, 144, 233, 265, 277, 459
SfaNI GCATC 1 cut(s) 643
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 7 cut(s) 157, 204, 330, 372, 484, 515, 562
SspI AATATT 2 cut(s) 384, 393
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 233
TaqI TCGA 4 cut(s) 247, 271, 280, 493
TasI AATT 7 cut(s) 157, 204, 330, 372, 484, 515, 562
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 137
TseI GCWGC 1 cut(s) 151
TspDTI ATGAA 6 cut(s) 426, 528, 630, 690, 705, 720
TspRI CASTG 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 2 cut(s) 112, 129
XspI CTAG 1 cut(s) 458
ZraI GACGTC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.