Rmu_sc0000932.1_g000010

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000932.1
OG0000697
Physical Location & Seq
Reverse (-)
53305 .. 54097
793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000932.1_g000010.1.cds

Sequence Viewer

Length: 702 bp
atggtgaagggacctgagataagtgcataccgaaacagatatggaaaagcatcacaaaatgtattggctgctactaactttgacttggaattcatttatgtgctcagtgggtgggaaggttcagctcatgattcaagagtactaaatgatgcattgacaaggagaaatgggcttaagttacctccaggtaaatttttattagtggatgctggatttgcaaatagacatggtttcttagcacctattagaggcgtccgctatcatctcaaagagttcagtggtcagggtcgccaccctgaaaatgcgcgtgagttattcaatcttcgtcatgcttccttgagaaacgtagttgagagaatgtttggagtacttaaatcccgtttcacaatattcaagactgcacctccattcccatttaagacacaagcggagctagtgttggcttgtgcaggactccacaactttcttcgtaaggaatgtcgttctgatttatttcctgttgaaccggaggatgaagaagattatgaacttgaagaagatccggaagatgaattccaaacccaagaccaacaaagagaggaagctaatgattggagaatggagatagctaatcatatgtggagagatgcaagacctgcacctgatcctgaacccaatcctccacccgttgttgaaggcaacaatgcagtagaacaatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

233

Amino Acids

26.68

Weight (kDa)

5.46

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 643
AccII CGCG 1 cut(s) 307
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 2 cut(s) 256, 428
AclWI GGATC 2 cut(s) 533, 638
AcsI RAATTY 3 cut(s) 89, 191, 551
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 141, 369
AfiI CCNNNNNNNGG 1 cut(s) 248
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 6 cut(s) 135, 319, 394, 503, 533, 674
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 4 cut(s) 125, 433, 584, 608
AluI AGCT 4 cut(s) 125, 433, 584, 608
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 2 cut(s) 533, 638
Aor13HI TCCGGA 1 cut(s) 541
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 3 cut(s) 89, 191, 551
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 186
BfaI CTAG 1 cut(s) 434
BfrI CTTAAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 643
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
BmcAI AGTACT 2 cut(s) 141, 369
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 4 cut(s) 59, 139, 196, 616
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 358
BsaHI GRCGYC 1 cut(s) 252
BsaWI WCCGGW 2 cut(s) 505, 541
BsaXI ACNNNNNCTCC 4 cut(s) 388, 418, 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 248
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 211, 517
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 2 cut(s) 6, 118
BseXI GCAGC 1 cut(s) 55
BsgI GTGCAG 3 cut(s) 384, 468, 621
Bsh1236I CGCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 2 cut(s) 506, 542
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 2 cut(s) 538, 643
BspACI CCGC 2 cut(s) 256, 428
BspCNI CTCAG 2 cut(s) 7, 117
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 307
BspHI TCATGA 2 cut(s) 127, 698
BspLI GGNNCC 1 cut(s) 12
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 2 cut(s) 533, 638
BspTI CTTAAG 1 cut(s) 173
BssMI GATC 2 cut(s) 538, 643
BssNI GRCGYC 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 635
BstDEI CTNAG 3 cut(s) 15, 104, 235
BstENI CCTNNNNNAGG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 211, 517
BstFNI CGCG 1 cut(s) 307
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 541, 646
BstMBI GATC 2 cut(s) 538, 643
BstMWI GCNNNNNNNGC 2 cut(s) 215, 635
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstUI CGCG 1 cut(s) 307
BstV1I GCAGC 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BtsCI GGATG 2 cut(s) 211, 517
BtsIMutI CAGTG 2 cut(s) 112, 283
BveI ACCTGC 1 cut(s) 643
CciI TCATGA 2 cut(s) 127, 698
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 140, 368
CviAII CATG 4 cut(s) 128, 227, 329, 699
CviJI RGCY 7 cut(s) 68, 125, 172, 433, 443, 584, 608
CviKI_1 RGCY 7 cut(s) 68, 125, 172, 433, 443, 584, 608
CviQI GTAC 2 cut(s) 140, 368
DdeI CTNAG 3 cut(s) 15, 104, 235
DpnI GATC 2 cut(s) 540, 645
DpnII GATC 2 cut(s) 538, 643
Eco47I GGWCC 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 11
EcoRI GAATTC 2 cut(s) 89, 551
EcoRII CCWGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 154
FaeI CATG 4 cut(s) 131, 230, 332, 702
FaqI GGGAC 1 cut(s) 24
FatI CATG 4 cut(s) 127, 226, 328, 698
FauNDI CATATG 1 cut(s) 615
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 218, 524
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 1 cut(s) 434
GlaI GCGC 1 cut(s) 306
GluI GCNGC 1 cut(s) 69
GsuI CTGGAG 1 cut(s) 168
HapII CCGG 2 cut(s) 506, 542
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 131, 230, 332, 702
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HinfI GANTC 2 cut(s) 131, 453
HpaII CCGG 2 cut(s) 506, 542
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 6 cut(s) 128, 135, 394, 542, 647, 699
HpyAV CCTTC 2 cut(s) 110, 668
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 8 cut(s) 26, 152, 218, 401, 449, 629, 638, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 635
HpyF3I CTNAG 3 cut(s) 15, 104, 235
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 131, 230, 332, 702
HspAI GCGC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 2 cut(s) 538, 643
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 4 cut(s) 59, 139, 196, 616
MaeI CTAG 1 cut(s) 434
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 177
MalI GATC 2 cut(s) 540, 645
MboI GATC 2 cut(s) 538, 643
MboII GAAGA 7 cut(s) 314, 458, 527, 530, 545, 548, 557
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 3 cut(s) 89, 191, 551
MlyI GAGTC 1 cut(s) 447
MnlI CCTC 6 cut(s) 192, 242, 414, 502, 571, 669
Mph1103I ATGCAT 1 cut(s) 154
MroI TCCGGA 1 cut(s) 541
MseI TTAA 3 cut(s) 174, 372, 417
MslI CAYNNNNRTG 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 2 cut(s) 506, 542
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 307
MwoI GCNNNNNNNGC 2 cut(s) 215, 635
NdeI CATATG 1 cut(s) 615
NdeII GATC 2 cut(s) 538, 643
NlaIII CATG 4 cut(s) 131, 230, 332, 702
NlaIV GGNNCC 1 cut(s) 12
NsiI ATGCAT 1 cut(s) 154
PagI TCATGA 2 cut(s) 127, 698
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 2 cut(s) 141, 369
RsaNI GTAC 2 cut(s) 140, 368
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 3 cut(s) 174, 372, 417
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 538, 643
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 2 cut(s) 141, 369
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 4 cut(s) 59, 139, 196, 616
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 173, 337
SmoI CTYRAG 2 cut(s) 173, 337
Sse9I AATT 3 cut(s) 89, 191, 551
SsiI CCGC 2 cut(s) 256, 428
SspI AATATT 1 cut(s) 390
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 184
TaiI ACGT 1 cut(s) 348
TasI AATT 3 cut(s) 89, 191, 551
TatI WGTACW 2 cut(s) 139, 367
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 3 cut(s) 174, 372, 417
Tru9I TTAA 3 cut(s) 174, 372, 417
TscAI CASTG 2 cut(s) 112, 283
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 4 cut(s) 82, 528, 540, 564
TspRI CASTG 2 cut(s) 112, 283
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 11
XagI CCTNNNNNAGG 1 cut(s) 246
XapI RAATTY 3 cut(s) 89, 191, 551
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 2 cut(s) 141, 369
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.