Rw0G016280

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0000697
Physical Location & Seq
Reverse (-)
191664 .. 193125
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016280.1

Sequence Viewer

Length: 876 bp
ATGTCTGAAATGGACATGTATGGAGCTGATGAAGAGGAAGAACAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAATACAAGCAGTGGTTTATGTGTTATACGACCTTGTATTCAGCATACGTGGTGAACGTATTAGACGTCCACTGACTCGGCGACCAGTGACATCAAGTGGATACATATATATGCACAAAATATTAGACAGAGACCCTCAAATCTTTAGAGAGGTGTATAGAATGTATCCTGACGTTTTTCGAAAATTATGTAGCATCCTAAATGAAGCTCGGCTGATATTTGAGCGATCTCATTTCACTGTTAGCAAAAGTTTCAACAAAGTCTTGAAGGCCTTGAATACAATAGCACCGGAGTTTATGGCTAAACCTGAGTCCATGCCACCCAACATAAGAGAAAGTACAAGGTTTTATCCTTACTTTAAGGATTGCGTCGGAGCTATAGATGGCACACATATTCCAGCAACGGTAGTTGGACGTGAGGTTAGCAGATATCGAAATCGACATGGGAAGATATCACAAAATGTATTAGCAGCTTGTAACTTTGATTTACAGTTCACATATGTAATTAGTGGATGGGAGGGTTCCGCTCATGATTCAAAAGTATTGAATGATGCGATTTCTAGACGAAATGGACTCAAAGAATGCCGTTCAGATGAATTTCCTCCCGAACCAGAAGAAGATCCGATGGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCAGCAGAGAGAAAATGCTAATGAATGGAGAATGAGTATTGCTACTCATATGTGGACAGATGCCCAACCAAATGCCAACAATGAAAACAATAACAATGAAGAAAGTGAAAATGAAGGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

291

Amino Acids

34.05

Weight (kDa)

4.97

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 152 - 209 3.5e-08 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 602
AciI CCGC 1 cut(s) 600
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 7 cut(s) 331, 343, 352, 612, 622, 725, 740
AjiI CACGTC 1 cut(s) 491
AluBI AGCT 4 cut(s) 26, 284, 452, 548
AluI AGCT 4 cut(s) 26, 284, 452, 548
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 689
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 671
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 256
BbvI GCAGC 1 cut(s) 557
BccI CCATC 3 cut(s) 452, 582, 694
BceAI ACGGC 1 cut(s) 645
BciVI GTATCC 2 cut(s) 170, 252
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 636
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 2 cut(s) 170, 252
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
BmgBI CACGTC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 598
BmsI GCATC 3 cut(s) 279, 616, 799
Bpu14I TTCGAA 1 cut(s) 256
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bse1I ACTGG 1 cut(s) 161
BseGI GGATG 3 cut(s) 270, 593, 736
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 557
BshFI GGCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 365
BsmAI GTCTC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 662
BsnI GGCC 1 cut(s) 347
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 3 cut(s) 302, 694, 748
BspACI CCGC 1 cut(s) 600
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 598
BspPI GGATC 1 cut(s) 689
BspT104I TTCGAA 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 602
BsrI ACTGG 1 cut(s) 161
BssMI GATC 3 cut(s) 302, 694, 748
BssNI GRCGYC 1 cut(s) 142
Bst4CI ACNGT 4 cut(s) 45, 316, 481, 567
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 142
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 270, 593, 736
BstKTI GATC 3 cut(s) 305, 697, 751
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 3 cut(s) 302, 694, 748
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 557
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BsuI GTATCC 2 cut(s) 170, 252
BsuRI GGCC 1 cut(s) 347
BtrI CACGTC 1 cut(s) 491
BtsCI GGATG 3 cut(s) 270, 593, 736
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 4 cut(s) 93, 146, 168, 312
CciI TCATGA 1 cut(s) 604
CseI GACGC 1 cut(s) 433
Csp6I GTAC 1 cut(s) 414
CviAII CATG 4 cut(s) 16, 391, 518, 605
CviJI RGCY 8 cut(s) 26, 56, 284, 289, 347, 377, 452, 548
CviKI_1 RGCY 8 cut(s) 26, 56, 284, 289, 347, 377, 452, 548
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 1 cut(s) 384
DpnI GATC 3 cut(s) 304, 696, 750
DpnII GATC 3 cut(s) 302, 694, 748
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 347
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 2 cut(s) 506, 528
EcoRV GATATC 2 cut(s) 506, 528
FaeI CATG 4 cut(s) 19, 394, 521, 608
FatI CATG 4 cut(s) 15, 390, 517, 604
FauNDI CATATG 2 cut(s) 574, 798
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 3 cut(s) 257, 600, 743
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 1 cut(s) 636
GluI GCNGC 1 cut(s) 546
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 365
HgaI GACGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 4 cut(s) 19, 394, 521, 608
HinfI GANTC 4 cut(s) 151, 386, 608, 648
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 4 cut(s) 131, 146, 570, 804
Hpy188I TCNGA 4 cut(s) 7, 449, 667, 699
Hpy188III TCNNGA 6 cut(s) 245, 340, 605, 636, 680, 710
Hpy8I GTNNAC 4 cut(s) 131, 146, 570, 804
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 337, 857
HpyCH4III ACNGT 4 cut(s) 45, 316, 481, 567
HpyCH4IV ACGT 5 cut(s) 124, 133, 142, 249, 490
HpyCH4V TGCA 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 5 cut(s) 124, 133, 142, 249, 490
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 4 cut(s) 19, 394, 521, 608
Kzo9I GATC 3 cut(s) 302, 694, 748
LmnI GCTCC 2 cut(s) 23, 449
LpnPI CCDG 6 cut(s) 174, 258, 378, 396, 486, 699
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 3 cut(s) 279, 616, 799
MaeI CTAG 1 cut(s) 636
MaeII ACGT 5 cut(s) 124, 133, 142, 249, 490
MaeIII GTNAC 2 cut(s) 163, 551
MalI GATC 3 cut(s) 304, 696, 750
MbiI CCGCTC 1 cut(s) 602
MboI GATC 3 cut(s) 302, 694, 748
MboII GAAGA 7 cut(s) 44, 50, 535, 701, 704, 725, 860
MflI RGATCY 1 cut(s) 694
MluCI AATT 4 cut(s) 260, 579, 671, 718
MlyI GAGTC 3 cut(s) 145, 395, 642
MmeI TCCRAC 2 cut(s) 427, 466
MnlI CCTC 7 cut(s) 28, 46, 220, 222, 487, 586, 687
MseI TTAA 3 cut(s) 60, 71, 435
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 662
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeI CATATG 2 cut(s) 574, 798
NdeII GATC 3 cut(s) 302, 694, 748
NlaIII CATG 4 cut(s) 19, 394, 521, 608
NlaIV GGNNCC 1 cut(s) 598
NmeAIII GCCGAG 2 cut(s) 133, 265
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 256
PagI TCATGA 1 cut(s) 604
PceI AGGCCT 1 cut(s) 347
PciI ACATGT 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 139
PctI GAATGC 1 cut(s) 662
PfeI GAWTC 1 cut(s) 608
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 3 cut(s) 145, 394, 642
PpsI GAGTC 3 cut(s) 145, 394, 642
Ppu21I YACGTR 1 cut(s) 125
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 598
PsuI RGATCY 1 cut(s) 694
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 3 cut(s) 60, 71, 435
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 3 cut(s) 302, 694, 748
SchI GAGTC 3 cut(s) 145, 395, 642
SfaNI GCATC 3 cut(s) 279, 616, 799
SfcI CTRYAG 1 cut(s) 453
SfuI TTCGAA 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 4 cut(s) 260, 579, 671, 718
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 1 cut(s) 600
SspI AATATT 1 cut(s) 198
SspMI CTAG 1 cut(s) 636
StuI AGGCCT 1 cut(s) 347
TaaI ACNGT 4 cut(s) 45, 316, 481, 567
TaiI ACGT 5 cut(s) 127, 136, 145, 252, 493
TaqI TCGA 3 cut(s) 256, 508, 514
TasI AATT 4 cut(s) 260, 579, 671, 718
TatI WGTACW 1 cut(s) 413
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 3 cut(s) 60, 71, 435
Tru9I TTAA 3 cut(s) 60, 71, 435
TscAI CASTG 4 cut(s) 93, 153, 168, 319
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 7 cut(s) 45, 294, 684, 786, 846, 861, 876
TspRI CASTG 4 cut(s) 93, 153, 168, 319
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 671
XbaI TCTAGA 1 cut(s) 635
XceI RCATGY 1 cut(s) 19
XspI CTAG 1 cut(s) 636
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.