nuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 120 |
| AccB1I | GGYRCC | 1 cut(s) | 736 |
| AccI | GTMKAC | 2 cut(s) | 573, 1709 |
| AccII | CGCG | 1 cut(s) | 245 |
| AciI | CCGC | 3 cut(s) | 243, 1561, 1669 |
| AclI | AACGTT | 1 cut(s) | 1492 |
| AclWI | GGATC | 2 cut(s) | 612, 1679 |
| AcoI | YGGCCR | 1 cut(s) | 1611 |
| AcsI | RAATTY | 8 cut(s) | 55, 578, 702, 711, 911, 1013, 1268, 1691 |
| AcuI | CTGAAG | 2 cut(s) | 297, 1320 |
| AfaI | GTAC | 4 cut(s) | 71, 760, 1077, 1876 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1586 |
| AflII | CTTAAG | 1 cut(s) | 1741 |
| AflIII | ACRYGT | 1 cut(s) | 188 |
| Alw21I | GWGCWC | 1 cut(s) | 1594 |
| Alw26I | GTCTC | 3 cut(s) | 41, 434, 1928 |
| AlwI | GGATC | 2 cut(s) | 612, 1679 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1406 |
| Ama87I | CYCGRG | 1 cut(s) | 1800 |
| AoxI | GGCC | 3 cut(s) | 560, 1611, 1751 |
| ApeKI | GCWGC | 4 cut(s) | 4, 333, 686, 890 |
| ApoI | RAATTY | 8 cut(s) | 55, 578, 702, 711, 911, 1013, 1268, 1691 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 26, 676 |
| AspLEI | GCGC | 2 cut(s) | 247, 1658 |
| AspS9I | GGNCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| AsuHPI | GGTGA | 3 cut(s) | 404, 491, 720 |
| AsuII | TTCGAA | 1 cut(s) | 108 |
| AvaI | CYCGRG | 1 cut(s) | 1800 |
| AvaII | GGWCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| BanI | GGYRCC | 1 cut(s) | 736 |
| BanII | GRGCYC | 1 cut(s) | 1799 |
| BbsI | GAAGAC | 1 cut(s) | 1820 |
| Bbv12I | GWGCWC | 1 cut(s) | 1594 |
| BbvI | GCAGC | 3 cut(s) | 345, 673, 877 |
| BccI | CCATC | 5 cut(s) | 733, 797, 927, 1785, 1897 |
| BceAI | ACGGC | 1 cut(s) | 1626 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 810, 844 |
| BcoDI | GTCTC | 3 cut(s) | 41, 434, 1928 |
| BfaI | CTAG | 5 cut(s) | 392, 657, 1022, 1283, 1325 |
| BfrI | CTTAAG | 1 cut(s) | 1741 |
| BisI | GCNGC | 4 cut(s) | 5, 334, 687, 891 |
| BlpI | GCTNAGC | 1 cut(s) | 1911 |
| BlsI | GCNGC | 4 cut(s) | 6, 335, 688, 892 |
| BmcAI | AGTACT | 1 cut(s) | 1876 |
| Bme18I | GGWCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| BmeT110I | CYCGRG | 1 cut(s) | 1800 |
| BmgT120I | GGNCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| BmiI | GGNNCC | 5 cut(s) | 367, 368, 738, 834, 1954 |
| BmsI | GCATC | 5 cut(s) | 520, 654, 961, 1393, 1897 |
| BpiI | GAAGAC | 1 cut(s) | 1820 |
| BpmI | CTGGAG | 1 cut(s) | 1659 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1596 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1911 |
| Bpu14I | TTCGAA | 1 cut(s) | 108 |
| BpuEI | CTTGAG | 2 cut(s) | 1135, 1323 |
| Bsa29I | ATCGAT | 2 cut(s) | 616, 1736 |
| BsaBI | GATNNNNATC | 1 cut(s) | 322 |
| BsaI | GGTCTC | 2 cut(s) | 41, 1928 |
| BsaWI | WCCGGW | 2 cut(s) | 32, 835 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1586 |
| Bse1I | ACTGG | 3 cut(s) | 563, 1463, 1929 |
| Bse3DI | GCAATG | 3 cut(s) | 526, 715, 828 |
| Bse8I | GATNNNNATC | 1 cut(s) | 322 |
| BseCI | ATCGAT | 2 cut(s) | 616, 1736 |
| BseGI | GGATG | 4 cut(s) | 938, 1796, 1858, 1912 |
| BseJI | GATNNNNATC | 1 cut(s) | 322 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1586 |
| BseMI | GCAATG | 3 cut(s) | 526, 715, 828 |
| BseMII | CTCAG | 1 cut(s) | 1885 |
| BseNI | ACTGG | 3 cut(s) | 563, 1463, 1929 |
| BseX3I | CGGCCG | 1 cut(s) | 1611 |
| BseXI | GCAGC | 3 cut(s) | 345, 673, 877 |
| BseYI | CCCAGC | 1 cut(s) | 1938 |
| BsgI | GTGCAG | 2 cut(s) | 1161, 1245 |
| Bsh1236I | CGCG | 1 cut(s) | 245 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1614 |
| BshFI | GGCC | 3 cut(s) | 562, 1613, 1753 |
| BshNI | GGYRCC | 1 cut(s) | 736 |
| BshVI | ATCGAT | 2 cut(s) | 616, 1736 |
| BsiEI | CGRYCG | 1 cut(s) | 1614 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1594 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1800 |
| BsiSI | CCGG | 2 cut(s) | 33, 836 |
| BslFI | GGGAC | 4 cut(s) | 27, 379, 846, 1938 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1586 |
| BsmAI | GTCTC | 3 cut(s) | 41, 434, 1928 |
| BsmFI | GGGAC | 4 cut(s) | 27, 379, 846, 1938 |
| BsmI | GAATGC | 1 cut(s) | 1419 |
| BsnI | GGCC | 3 cut(s) | 562, 1613, 1753 |
| Bso31I | GGTCTC | 2 cut(s) | 41, 1928 |
| BsoBI | CYCGRG | 1 cut(s) | 1800 |
| Bsp119I | TTCGAA | 1 cut(s) | 108 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1594, 1799 |
| Bsp143I | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1911 |
| BspACI | CCGC | 3 cut(s) | 243, 1561, 1669 |
| BspANI | GGCC | 3 cut(s) | 562, 1613, 1753 |
| BspCNI | CTCAG | 1 cut(s) | 1884 |
| BspDI | ATCGAT | 2 cut(s) | 616, 1736 |
| BspFNI | CGCG | 1 cut(s) | 245 |
| BspHI | TCATGA | 1 cut(s) | 949 |
| BspLI | GGNNCC | 5 cut(s) | 367, 368, 738, 834, 1954 |
| BspPI | GGATC | 2 cut(s) | 612, 1679 |
| BspT104I | TTCGAA | 1 cut(s) | 108 |
| BspT107I | GGYRCC | 1 cut(s) | 736 |
| BspTI | CTTAAG | 1 cut(s) | 1741 |
| BspTNI | GGTCTC | 2 cut(s) | 41, 1928 |
| BsrDI | GCAATG | 3 cut(s) | 526, 715, 828 |
| BsrI | ACTGG | 3 cut(s) | 563, 1463, 1929 |
| BssMI | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| BssNAI | GTATAC | 1 cut(s) | 1710 |
| Bst1107I | GTATAC | 1 cut(s) | 1710 |
| Bst4CI | ACNGT | 4 cut(s) | 296, 472, 736, 1882 |
| BstAFI | CTTAAG | 1 cut(s) | 1741 |
| BstBI | TTCGAA | 1 cut(s) | 108 |
| BstC8I | GCNNGC | 3 cut(s) | 847, 1378, 1751 |
| BstDEI | CTNAG | 5 cut(s) | 926, 1596, 1823, 1871, 1911 |
| BstF5I | GGATG | 4 cut(s) | 938, 1796, 1858, 1912 |
| BstFNI | CGCG | 1 cut(s) | 245 |
| BstHHI | GCGC | 2 cut(s) | 247, 1658 |
| BstKTI | GATC | 8 cut(s) | 283, 616, 620, 859, 1066, 1687, 1736, 1740 |
| BstMAI | GTCTC | 3 cut(s) | 41, 434, 1928 |
| BstMBI | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| BstMCI | CGRYCG | 1 cut(s) | 1614 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 692, 1373, 1382, 1634, 1817 |
| BstNSI | RCATGY | 3 cut(s) | 192, 1082, 1109 |
| BstUI | CGCG | 1 cut(s) | 245 |
| BstV1I | GCAGC | 3 cut(s) | 345, 673, 877 |
| BstV2I | GAAGAC | 1 cut(s) | 1820 |
| BstX2I | RGATCY | 1 cut(s) | 1684 |
| BstYI | RGATCY | 1 cut(s) | 1684 |
| BstZ17I | GTATAC | 1 cut(s) | 1710 |
| BstZI | CGGCCG | 1 cut(s) | 1611 |
| Bsu15I | ATCGAT | 2 cut(s) | 616, 1736 |
| BsuRI | GGCC | 3 cut(s) | 562, 1613, 1753 |
| BsuTUI | ATCGAT | 2 cut(s) | 616, 1736 |
| BtgZI | GCGATG | 1 cut(s) | 1905 |
| BtsCI | GGATG | 4 cut(s) | 938, 1796, 1858, 1912 |
| BtsI | GCAGTG | 1 cut(s) | 1472 |
| BtsIMutI | CAGTG | 4 cut(s) | 172, 696, 1472, 1936 |
| Cac8I | GCNNGC | 3 cut(s) | 847, 1378, 1751 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1406 |
| CciI | TCATGA | 1 cut(s) | 949 |
| CfoI | GCGC | 2 cut(s) | 247, 1658 |
| Cfr13I | GGNCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| ClaI | ATCGAT | 2 cut(s) | 616, 1736 |
| Csp6I | GTAC | 4 cut(s) | 70, 759, 1076, 1875 |
| CviQI | GTAC | 4 cut(s) | 70, 759, 1076, 1875 |
| DdeI | CTNAG | 5 cut(s) | 926, 1596, 1823, 1871, 1911 |
| DpnI | GATC | 8 cut(s) | 282, 615, 619, 858, 1065, 1686, 1735, 1739 |
| DpnII | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| EaeI | YGGCCR | 1 cut(s) | 1611 |
| EagI | CGGCCG | 1 cut(s) | 1611 |
| EciI | GGCGGA | 2 cut(s) | 1576, 1684 |
| EclXI | CGGCCG | 1 cut(s) | 1611 |
| Eco24I | GRGCYC | 1 cut(s) | 1799 |
| Eco31I | GGTCTC | 2 cut(s) | 41, 1928 |
| Eco47I | GGWCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| Eco52I | CGGCCG | 1 cut(s) | 1611 |
| Eco57I | CTGAAG | 2 cut(s) | 297, 1320 |
| Eco88I | CYCGRG | 1 cut(s) | 1800 |
| EcoO109I | RGGNCCY | 1 cut(s) | 366 |
| EcoRI | GAATTC | 2 cut(s) | 711, 911 |
| EcoT22I | ATGCAT | 2 cut(s) | 647, 1369 |
| EcoT38I | GRGCYC | 1 cut(s) | 1799 |
| FaqI | GGGAC | 4 cut(s) | 27, 379, 846, 1938 |
| FauI | CCCGC | 1 cut(s) | 250 |
| FauNDI | CATATG | 1 cut(s) | 1584 |
| FblI | GTMKAC | 2 cut(s) | 573, 1709 |
| Fnu4HI | GCNGC | 4 cut(s) | 5, 334, 687, 891 |
| FokI | GGATG | 4 cut(s) | 945, 1803, 1865, 1919 |
| FriOI | GRGCYC | 1 cut(s) | 1799 |
| Fsp4HI | GCNGC | 4 cut(s) | 5, 334, 687, 891 |
| FspBI | CTAG | 5 cut(s) | 392, 657, 1022, 1283, 1325 |
| GlaI | GCGC | 2 cut(s) | 246, 1657 |
| GluI | GCNGC | 4 cut(s) | 5, 334, 687, 891 |
| GsaI | CCCAGC | 1 cut(s) | 1942 |
| GsuI | CTGGAG | 1 cut(s) | 1659 |
| HaeIII | GGCC | 3 cut(s) | 562, 1613, 1753 |
| HapII | CCGG | 2 cut(s) | 33, 836 |
| HhaI | GCGC | 2 cut(s) | 247, 1658 |
| Hin6I | GCGC | 2 cut(s) | 245, 1656 |
| HinP1I | GCGC | 2 cut(s) | 245, 1656 |
| HinfI | GANTC | 7 cut(s) | 130, 421, 677, 953, 1295, 1552, 1622 |
| HpaII | CCGG | 2 cut(s) | 33, 836 |
| HphI | GGTGA | 3 cut(s) | 404, 491, 720 |
| Hpy166II | GTNNAC | 6 cut(s) | 386, 574, 1398, 1470, 1529, 1710 |
| Hpy188I | TCNGA | 3 cut(s) | 569, 1264, 1300 |
| Hpy8I | GTNNAC | 6 cut(s) | 386, 574, 1398, 1470, 1529, 1710 |
| Hpy99I | CGWCG | 1 cut(s) | 605 |
| HpyAV | CCTTC | 3 cut(s) | 60, 304, 1001 |
| HpyCH4III | ACNGT | 4 cut(s) | 296, 472, 736, 1882 |
| HpyCH4IV | ACGT | 4 cut(s) | 600, 1172, 1429, 1492 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 692, 1373, 1382, 1634, 1817 |
| HpyF3I | CTNAG | 5 cut(s) | 926, 1596, 1823, 1871, 1911 |
| HpySE526I | ACGT | 4 cut(s) | 600, 1172, 1429, 1492 |
| HspAI | GCGC | 2 cut(s) | 245, 1656 |
| KflI | GGGWCCC | 1 cut(s) | 366 |
| Kzo9I | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| LmnI | GCTCC | 2 cut(s) | 19, 1597 |
| Lsp1109I | GCAGC | 3 cut(s) | 345, 673, 877 |
| LweI | GCATC | 5 cut(s) | 520, 654, 961, 1393, 1897 |
| MaeI | CTAG | 5 cut(s) | 392, 657, 1022, 1283, 1325 |
| MaeII | ACGT | 4 cut(s) | 600, 1172, 1429, 1492 |
| MaeIII | GTNAC | 6 cut(s) | 194, 372, 479, 896, 1067, 1575 |
| MalI | GATC | 8 cut(s) | 282, 615, 619, 858, 1065, 1686, 1735, 1739 |
| MboI | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| MfeI | CAATTG | 1 cut(s) | 690 |
| MflI | RGATCY | 1 cut(s) | 1684 |
| MhlI | GDGCHC | 2 cut(s) | 1594, 1799 |
| MlyI | GAGTC | 2 cut(s) | 430, 947 |
| MmeI | TCCRAC | 1 cut(s) | 649 |
| MnlI | CCTC | 9 cut(s) | 94, 594, 647, 931, 978, 1125, 1191, 1579, 1878 |
| Mph1103I | ATGCAT | 2 cut(s) | 647, 1369 |
| MroXI | GAANNNNTTC | 2 cut(s) | 26, 676 |
| MseI | TTAA | 8 cut(s) | 288, 780, 996, 1194, 1632, 1647, 1680, 1742 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1083, 1587 |
| MspCI | CTTAAG | 1 cut(s) | 1741 |
| MspI | CCGG | 2 cut(s) | 33, 836 |
| MunI | CAATTG | 1 cut(s) | 690 |
| Mva1269I | GAATGC | 1 cut(s) | 1419 |
| MvnI | CGCG | 1 cut(s) | 245 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 692, 1373, 1382, 1634, 1817 |
| NdeI | CATATG | 1 cut(s) | 1584 |
| NdeII | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| NlaIV | GGNNCC | 5 cut(s) | 367, 368, 738, 834, 1954 |
| NmuCI | GTSAC | 3 cut(s) | 372, 479, 1067 |
| NsiI | ATGCAT | 2 cut(s) | 647, 1369 |
| NspI | RCATGY | 3 cut(s) | 192, 1082, 1109 |
| NspV | TTCGAA | 1 cut(s) | 108 |
| PaeR7I | CTCGAG | 1 cut(s) | 1800 |
| PagI | TCATGA | 1 cut(s) | 949 |
| PciI | ACATGT | 1 cut(s) | 188 |
| PctI | GAATGC | 1 cut(s) | 1419 |
| PdmI | GAANNNNTTC | 2 cut(s) | 26, 676 |
| PfeI | GAWTC | 5 cut(s) | 130, 677, 1295, 1552, 1622 |
| PflFI | GACNNNGTC | 1 cut(s) | 370 |
| PkrI | GCNGC | 4 cut(s) | 6, 335, 688, 892 |
| PleI | GAGTC | 2 cut(s) | 429, 947 |
| PpsI | GAGTC | 2 cut(s) | 429, 947 |
| PpuMI | RGGWCCY | 1 cut(s) | 366 |
| PscI | ACATGT | 1 cut(s) | 188 |
| PsiI | TTATAA | 1 cut(s) | 120 |
| Psp1406I | AACGTT | 1 cut(s) | 1492 |
| Psp5II | RGGWCCY | 1 cut(s) | 366 |
| PspFI | CCCAGC | 1 cut(s) | 1938 |
| PspN4I | GGNNCC | 5 cut(s) | 367, 368, 738, 834, 1954 |
| PspPI | GGNCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| PspPPI | RGGWCCY | 1 cut(s) | 366 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1406 |
| PsuI | RGATCY | 1 cut(s) | 1684 |
| PsyI | GACNNNGTC | 1 cut(s) | 370 |
| RsaI | GTAC | 4 cut(s) | 71, 760, 1077, 1876 |
| RsaNI | GTAC | 4 cut(s) | 70, 759, 1076, 1875 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1083, 1587 |
| SaqAI | TTAA | 8 cut(s) | 288, 780, 996, 1194, 1632, 1647, 1680, 1742 |
| SatI | GCNGC | 4 cut(s) | 5, 334, 687, 891 |
| Sau3AI | GATC | 8 cut(s) | 280, 613, 617, 856, 1063, 1684, 1733, 1737 |
| Sau96I | GGNCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| ScaI | AGTACT | 1 cut(s) | 1876 |
| SchI | GAGTC | 2 cut(s) | 430, 947 |
| SduI | GDGCHC | 2 cut(s) | 1594, 1799 |
| SfaNI | GCATC | 5 cut(s) | 520, 654, 961, 1393, 1897 |
| Sfr274I | CTCGAG | 1 cut(s) | 1800 |
| SfuI | TTCGAA | 1 cut(s) | 108 |
| SinI | GGWCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| SlaI | CTCGAG | 1 cut(s) | 1800 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1083, 1587 |
| SmlI | CTYRAG | 4 cut(s) | 1114, 1338, 1741, 1800 |
| SmoI | CTYRAG | 4 cut(s) | 1114, 1338, 1741, 1800 |
| SsiI | CCGC | 3 cut(s) | 243, 1561, 1669 |
| SspI | AATATT | 1 cut(s) | 113 |
| SspMI | CTAG | 5 cut(s) | 392, 657, 1022, 1283, 1325 |
| TaaI | ACNGT | 4 cut(s) | 296, 472, 736, 1882 |
| TaiI | ACGT | 4 cut(s) | 603, 1175, 1432, 1495 |
| TatI | WGTACW | 2 cut(s) | 758, 1874 |
| TfiI | GAWTC | 5 cut(s) | 130, 677, 1295, 1552, 1622 |
| Tru1I | TTAA | 8 cut(s) | 288, 780, 996, 1194, 1632, 1647, 1680, 1742 |
| Tru9I | TTAA | 8 cut(s) | 288, 780, 996, 1194, 1632, 1647, 1680, 1742 |
| TscAI | CASTG | 4 cut(s) | 172, 703, 1479, 1936 |
| TseFI | GTSAC | 3 cut(s) | 372, 479, 1067 |
| TseI | GCWGC | 4 cut(s) | 4, 333, 686, 890 |
| Tsp45I | GTSAC | 3 cut(s) | 372, 479, 1067 |
| TspGWI | ACGGA | 3 cut(s) | 29, 162, 498 |
| TspRI | CASTG | 4 cut(s) | 172, 703, 1479, 1936 |
| Tth111I | GACNNNGTC | 1 cut(s) | 370 |
| Vha464I | CTTAAG | 1 cut(s) | 1741 |
| VpaK11BI | GGWCC | 5 cut(s) | 170, 366, 833, 1671, 1952 |
| XapI | RAATTY | 8 cut(s) | 55, 578, 702, 711, 911, 1013, 1268, 1691 |
| XceI | RCATGY | 3 cut(s) | 192, 1082, 1109 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1946 |
| XhoI | CTCGAG | 1 cut(s) | 1800 |
| XmiI | GTMKAC | 2 cut(s) | 573, 1709 |
| XmnI | GAANNNNTTC | 2 cut(s) | 26, 676 |
| XspI | CTAG | 5 cut(s) | 392, 657, 1022, 1283, 1325 |
| ZrmI | AGTACT | 1 cut(s) | 1876 |
| Zsp2I | ATGCAT | 2 cut(s) | 647, 1369 |