MD12G1024500.v1.1

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000697
Physical Location & Seq
Forward (+)
2623040 .. 2623708
669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024500.v1.1.491

Sequence Viewer

Length: 231 bp
ATGAAAACACAAGCAGAGTTAGTGTTAGCTTGTGCAGGATTGCACAACTTTCTTCGCAAAGAGTGTCGTTCTGATGAATTTCCAATCAAACAAGAAGATGATGAATTTGAAAATGATGATGAACTAGGTAATCAAACTCAAGAGCAACGGCGACAGATTGCTAATGCATGGAGAGCTAGCATAGCTACAAATATGTGGACAGATGCTATGAGTGAGAGCATTCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

77

Amino Acids

8.86

Weight (kDa)

4.61

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 77, 104
AgsI TTSAA 2 cut(s) 110, 224
AluBI AGCT 3 cut(s) 29, 176, 185
AluI AGCT 3 cut(s) 29, 176, 185
ApoI RAATTY 2 cut(s) 77, 104
AsuNHI GCTAGC 1 cut(s) 176
BceAI ACGGC 1 cut(s) 164
BfaI CTAG 2 cut(s) 125, 177
BmsI GCATC 1 cut(s) 193
BmtI GCTAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 123
BsgI GTGCAG 1 cut(s) 54
BsmI GAATGC 1 cut(s) 219
BspOI GCTAGC 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 173, 182
Cac8I GCNNGC 1 cut(s) 178
CviAII CATG 1 cut(s) 168
CviJI RGCY 3 cut(s) 29, 176, 185
CviKI_1 RGCY 3 cut(s) 29, 176, 185
EcoT22I ATGCAT 1 cut(s) 169
FaeI CATG 1 cut(s) 171
FaiI YATR 4 cut(s) 169, 182, 194, 209
FatI CATG 1 cut(s) 167
FspBI CTAG 2 cut(s) 125, 177
FspEI CC 6 cut(s) 21, 96, 111, 133, 154, 181
Hin1II CATG 1 cut(s) 171
Hpy166II GTNNAC 1 cut(s) 198
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 198
HpyCH4V TGCA 3 cut(s) 35, 43, 167
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 182
Hsp92II CATG 1 cut(s) 171
LpnPI CCDG 1 cut(s) 21
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 125, 177
MboII GAAGA 2 cut(s) 44, 107
MluCI AATT 2 cut(s) 77, 104
Mph1103I ATGCAT 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 173, 182
NheI GCTAGC 1 cut(s) 176
NlaIII CATG 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 169
PctI GAATGC 1 cut(s) 219
SetI ASST 4 cut(s) 31, 130, 178, 187
SfaNI GCATC 1 cut(s) 193
SgeI CNNG 8 cut(s) 23, 42, 48, 104, 137, 152, 180, 189
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 2 cut(s) 77, 104
SspMI CTAG 2 cut(s) 125, 177
TasI AATT 2 cut(s) 77, 104
TspDTI ATGAA 4 cut(s) 17, 90, 117, 135
XapI RAATTY 2 cut(s) 77, 104
XspI CTAG 2 cut(s) 125, 177
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.