Rroxscaffold_5G00335150

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000697
Physical Location & Seq
Forward (+)
2607685 .. 2609959
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00335150.1

Sequence Viewer

Length: 294 bp
ATGTCTGAAATTGATATGTATGGAGCTGATAAAGAGGAAGAACAGTTTTACAAGACTGTTAAGATATTATTAATGGCAATACAAGCTGTGGTTTATGTGTTATACGACCTTGTGTTCAGCATACGTGGTGAACGTATTAGACGTCCACCGACTCGGCGACCAGGGGGCTATGGATATCTTCCAAGACTCGCAAACAGCAAGGAAAGTATATTATTGTACTTTGAAAGTGGCGCACGAATAGGTCTTAAGGCCTTGGTTCTACTTCTATTGTGCTGCAATAGGTCAAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

97

Amino Acids

11.2

Weight (kDa)

9.43

Isoelectric Point (pI)

59.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 218
AflII CTTAAG 1 cut(s) 245
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 2 cut(s) 26, 86
AluI AGCT 2 cut(s) 26, 86
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 273
AseI ATTAAT 1 cut(s) 71
AspLEI GCGC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 140
BbvI GCAGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 245
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 161, 252
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 161, 252
BseXI GCAGC 1 cut(s) 260
BshFI GGCC 1 cut(s) 251
BsnI GGCC 1 cut(s) 251
BspANI GGCC 1 cut(s) 251
BspTI CTTAAG 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 161, 252
BssNI GRCGYC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 45, 58
BstACI GRCGYC 1 cut(s) 142
BstAFI CTTAAG 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 125
BstHHI GCGC 1 cut(s) 233
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 251
CfoI GCGC 1 cut(s) 233
Csp6I GTAC 1 cut(s) 217
CviJI RGCY 4 cut(s) 26, 86, 168, 251
CviKI_1 RGCY 4 cut(s) 26, 86, 168, 251
CviQI GTAC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 251
Eco32I GATATC 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaiI YATR 7 cut(s) 17, 21, 96, 103, 122, 171, 209
Fnu4HI GCNGC 1 cut(s) 274
Fsp4HI GCNGC 1 cut(s) 274
GlaI GCGC 1 cut(s) 232
GluI GCNGC 1 cut(s) 274
HaeIII GGCC 1 cut(s) 251
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 142
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HinfI GANTC 2 cut(s) 151, 186
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 131, 146
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 1 cut(s) 285
Hpy8I GTNNAC 2 cut(s) 131, 146
HpyCH4III ACNGT 2 cut(s) 45, 58
HpyCH4IV ACGT 3 cut(s) 124, 133, 142
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpySE526I ACGT 3 cut(s) 124, 133, 142
Hsp92I GRCGYC 1 cut(s) 142
HspAI GCGC 1 cut(s) 231
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 2 cut(s) 147, 174
Lsp1109I GCAGC 1 cut(s) 260
MaeII ACGT 3 cut(s) 124, 133, 142
MboII GAAGA 2 cut(s) 50, 170
MluCI AATT 1 cut(s) 9
MlyI GAGTC 2 cut(s) 145, 180
MnlI CCTC 1 cut(s) 28
MseI TTAA 3 cut(s) 60, 71, 246
MspCI CTTAAG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 83
NmeAIII GCCGAG 1 cut(s) 133
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 139
PkrI GCNGC 1 cut(s) 275
PleI GAGTC 2 cut(s) 145, 180
PpsI GAGTC 2 cut(s) 145, 180
Ppu21I YACGTR 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SaqAI TTAA 3 cut(s) 60, 71, 246
SatI GCNGC 1 cut(s) 274
SchI GAGTC 2 cut(s) 145, 180
ScrFI CCNGG 1 cut(s) 162
SetI ASST 8 cut(s) 28, 88, 111, 127, 136, 145, 244, 284
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 1 cut(s) 9
SseBI AGGCCT 1 cut(s) 251
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 45, 58
TaiI ACGT 3 cut(s) 127, 136, 145
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 216
Tru1I TTAA 3 cut(s) 60, 71, 246
Tru9I TTAA 3 cut(s) 60, 71, 246
TseI GCWGC 1 cut(s) 273
Vha464I CTTAAG 1 cut(s) 245
VspI ATTAAT 1 cut(s) 71
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.