Rw1G017460

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000697
Physical Location & Seq
Forward (+)
38588728 .. 38590200
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017460.1

Sequence Viewer

Length: 870 bp
ATGTCTGAAATGGACATGTATGGAGCTGATGAAGAGGAAGAGCAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAATACAAGCAGTGATTTATGTGTTATACGAACTTGTGTTCAACATAAGTGGTGAACATATTAGACGTCCGCTGACTCGTCGACCAGTGACATCAAGTGGATACATATATATGCACAACATATTGGACAGAGACCCTCAAATCTTTAGAGATGTGTATAGAATGTATCCTGACGTGTTTCGAAAATTATGTAGCATCCTAAAAGCGAAAACACCTTTACGGGATACAAGACACATTTGTCAGAAGTTTCAAAAAGTCTTGAAGGCCTTGAATACAATAGCACCACAGTTTATGGCTAAACCTGAGTCCATACCACCTAACATAAGAGAAAGTACAAGGTTCTATCCTTACTTTAAGGATTGTGTCGGAGCTATAGATGGCACGCATATTCCTGCAACGGTAGTTGGACGTGAGGTAAGCAGATATCGAAATCGCCATGGGAAGATATCACAAAATGTATTAGCAGCTTATAACTTTGATTTACAGTTCATATATGTGATTAGTGGATGGGAGGGTTCCGCTCATGATTCAAAAGTGTTGAATGATGCGATTTTTAGACGAAATGGACTCAAAGAATGTCGTTCAGATGAATTTCCTCCTGAACCAGAAGAAGATCCGATAGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCAGCAAAGAGAGAATGCTAATGAATGGAGAATGAGTATTGCTACTCATATGTGGACAGATGCCCAACCAAATGCCAACAATGAAAACAATGACAATCAAGAAAGTGAAAATGAGAGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

289

Amino Acids

33.97

Weight (kDa)

5.1

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 58 - 105 3e-07 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 150 - 212 9.5e-08 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
AasI GACNNNNNNGTC 1 cut(s) 312
AatII GACGTC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 596
AccI GTMKAC 1 cut(s) 157
AciI CCGC 2 cut(s) 146, 594
AclWI GGATC 1 cut(s) 683
AcsI RAATTY 1 cut(s) 665
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 409
AflIII ACRYGT 2 cut(s) 15, 249
AgsI TTSAA 8 cut(s) 118, 326, 337, 346, 606, 616, 719, 734
AjiI CACGTC 2 cut(s) 250, 485
AluBI AGCT 3 cut(s) 26, 446, 542
AluI AGCT 3 cut(s) 26, 446, 542
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 683
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 1 cut(s) 665
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 256
BbvI GCAGC 1 cut(s) 551
BccI CCATC 2 cut(s) 446, 576
BciVI GTATCC 3 cut(s) 170, 252, 292
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 447
BfuI GTATCC 3 cut(s) 170, 252, 292
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
BmgBI CACGTC 2 cut(s) 250, 485
BmiI GGNNCC 1 cut(s) 592
BmsI GCATC 3 cut(s) 279, 610, 793
Bpu14I TTCGAA 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bse1I ACTGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 3 cut(s) 270, 587, 730
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 551
BshFI GGCC 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 1 cut(s) 341
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 688, 742
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 2 cut(s) 146, 594
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 592
BspPI GGATC 1 cut(s) 683
BspQI GCTCTTC 1 cut(s) 33
BspT104I TTCGAA 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 596
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 2 cut(s) 688, 742
BssNI GRCGYC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 511
Bst4CI ACNGT 3 cut(s) 363, 475, 561
Bst6I CTCTTC 2 cut(s) 27, 33
BstACI GRCGYC 1 cut(s) 142
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 3 cut(s) 270, 587, 730
BstKTI GATC 2 cut(s) 691, 745
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 688, 742
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
BsuI GTATCC 3 cut(s) 170, 252, 292
BsuRI GGCC 1 cut(s) 341
BtgI CCRYGG 1 cut(s) 511
BtrI CACGTC 2 cut(s) 250, 485
BtsCI GGATG 3 cut(s) 270, 587, 730
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 168
Cac8I GCNNGC 1 cut(s) 458
CciI TCATGA 1 cut(s) 598
Csp6I GTAC 1 cut(s) 408
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 3 cut(s) 16, 512, 599
CviJI RGCY 6 cut(s) 26, 56, 341, 371, 446, 542
CviKI_1 RGCY 6 cut(s) 26, 56, 341, 371, 446, 542
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 1 cut(s) 378
DpnI GATC 2 cut(s) 690, 744
DpnII GATC 2 cut(s) 688, 742
DrdI GACNNNNNNGTC 1 cut(s) 312
DseDI GACNNNNNNGTC 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 27, 33
EarI CTCTTC 2 cut(s) 27, 33
Eco130I CCWWGG 1 cut(s) 511
Eco147I AGGCCT 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 2 cut(s) 500, 522
EcoRV GATATC 2 cut(s) 500, 522
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 3 cut(s) 19, 515, 602
FatI CATG 3 cut(s) 15, 511, 598
FauNDI CATATG 1 cut(s) 792
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 3 cut(s) 257, 594, 737
Fsp4HI GCNGC 1 cut(s) 540
GluI GCNGC 1 cut(s) 540
HaeIII GGCC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 3 cut(s) 19, 515, 602
HincII GTYRAC 1 cut(s) 158
HindII GTYRAC 1 cut(s) 158
HinfI GANTC 4 cut(s) 151, 380, 602, 642
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 3 cut(s) 131, 158, 798
Hpy188I TCNGA 5 cut(s) 7, 318, 443, 661, 693
Hpy188III TCNNGA 6 cut(s) 245, 334, 599, 674, 704, 842
Hpy8I GTNNAC 3 cut(s) 131, 158, 798
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 3 cut(s) 363, 475, 561
HpyCH4IV ACGT 3 cut(s) 142, 249, 484
HpyCH4V TGCA 2 cut(s) 190, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 3 cut(s) 142, 249, 484
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 3 cut(s) 19, 515, 602
Kzo9I GATC 2 cut(s) 688, 742
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 23, 443
LpnPI CCDG 6 cut(s) 174, 258, 390, 480, 687, 693
Lsp1109I GCAGC 1 cut(s) 551
LweI GCATC 3 cut(s) 279, 610, 793
MaeII ACGT 3 cut(s) 142, 249, 484
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 690, 744
MbiI CCGCTC 1 cut(s) 596
MboI GATC 2 cut(s) 688, 742
MboII GAAGA 6 cut(s) 44, 50, 529, 695, 698, 719
MflI RGATCY 1 cut(s) 688
MluCI AATT 3 cut(s) 260, 665, 712
MlyI GAGTC 3 cut(s) 145, 389, 636
MmeI TCCRAC 2 cut(s) 421, 460
MnlI CCTC 6 cut(s) 28, 46, 222, 481, 580, 681
MseI TTAA 3 cut(s) 60, 71, 429
MslI CAYNNNNRTG 2 cut(s) 185, 569
MspA1I CMGCKG 1 cut(s) 148
Mva1269I GAATGC 1 cut(s) 763
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 1 cut(s) 792
NdeII GATC 2 cut(s) 688, 742
NlaIII CATG 3 cut(s) 19, 515, 602
NlaIV GGNNCC 1 cut(s) 592
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 256
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 1 cut(s) 341
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 33
PctI GAATGC 1 cut(s) 763
PfeI GAWTC 1 cut(s) 602
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 3 cut(s) 145, 388, 636
PpsI GAGTC 3 cut(s) 145, 388, 636
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PsiI TTATAA 1 cut(s) 546
PspN4I GGNNCC 1 cut(s) 592
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
RseI CAYNNNNRTG 2 cut(s) 185, 569
SalI GTCGAC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 60, 71, 429
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 2 cut(s) 688, 742
SchI GAGTC 3 cut(s) 145, 389, 636
SfaNI GCATC 3 cut(s) 279, 610, 793
SfcI CTRYAG 1 cut(s) 447
SfuI TTCGAA 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 185, 569
Sse9I AATT 3 cut(s) 260, 665, 712
SseBI AGGCCT 1 cut(s) 341
SsiI CCGC 2 cut(s) 146, 594
StuI AGGCCT 1 cut(s) 341
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 363, 475, 561
TaiI ACGT 3 cut(s) 145, 252, 487
TaqI TCGA 3 cut(s) 157, 256, 502
TasI AATT 3 cut(s) 260, 665, 712
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 3 cut(s) 60, 71, 429
Tru9I TTAA 3 cut(s) 60, 71, 429
TscAI CASTG 2 cut(s) 93, 168
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 45, 553, 678, 780, 840
TspRI CASTG 2 cut(s) 93, 168
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 665
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 157
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.