Rw4G006850
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000697
Physical Location & Seq
Reverse (-)
14300950 .. 14302284
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006850.1

Sequence Viewer

Length: 1161 bp
ATGTCCATCCAAAATCATAGACGTATTGAACGTCCACTAACTCGAAGACCAGTTACTACAGATGGATACATATATATAAACAAGATTTTAGATGAAGACCCTAGAGTGTTTAGACGTGTGTACAGAATGTTTCCTGATGTGTTTCGAAAACTATGCAGTATTATCAGACAGAAAACACTTCTTCGAGACACAAGACATATTTGCATCGAAGAAATGCTTGCAATTTTTCTACTCACTATCGGGCAAAATAATCGATATTCGCAGGCTATCTTGACATTTCGTCGCTCTCATTACACTTGTAGCAGAAGCTTCAATAAAGTTCTTAAAGCATTGAATACGTTAGCTCCAGAGCTAATGGTCAAACCTGTGTCTGTGCAAGCTAAAATAAGAGAAAGTACAAGGTTTTATCCTTACTTTAAGGACTGTATAGGAGCAATTGATGGGACACATATTCCAGCAATGGTGAAGGGACCTGAGATAAGCGCATACCGAAACCGATATGGAAAAACATCACAAAATGTATTGGCTGCTACTAACTTTGACTTGGAATTCATTTATGTGCTCAGTGGGTGGGAAGGTTCAGCTCATGATTCAAGAGTACTAAATGATGCATTGACAAGGAGAAATGGGCTTAAGTTACCTCCAGGTAAATTTTTATTGGTGGATGCTGGATTTGCAAATAGACATGGTTTCTTAGCACCTATTAGAGGCATCCGCTATCATCTCAAAGAGATCAGTGGTCAGGGTCGCCACCCTGAAAATGCGCGTGAGTTATTCAATGTTCGTCATGCTTCCTTGAGAAACGTAGTTGAGAGAATATTTGGAGTACTTAAATCTCGTTTCACAATATTCAAGACTGCACCTCCATTCCCATTTAAGACACAAGCGGAGCTAGTGTTGGCTTGTGCAGGACTTCACAACTTTCTTCGTAAGGAATGTCGTTCTGATTTATTTCCTGTTGAACCGGAGGATGAAGAAGATTATGAATATGAAGAAGATCCGGAAGATGAATTCCAAACCCAAGACCAACAAAGAGAGGAAGCTAATGATTGGAGAATGGAGATAGCTAATCATATGTGGAGAGATGCAAGACCTGCACCTGATCCTGAACCCAATCCTCCACCCGTTGTTGAAGGCAACAATGCAGCAGAACAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

386

Amino Acids

44.77

Weight (kDa)

8.92

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 21 - 111 4.8e-24 Domain of unknown function (DUF8040)
DDE_Tnp_1 PF01609 140 - 301 9.2e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 146 - 307 1e-22 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1102
AccII CGCG 1 cut(s) 766
AccIII TCCGGA 1 cut(s) 1000
AciI CCGC 2 cut(s) 715, 887
AclWI GGATC 2 cut(s) 992, 1097
AcsI RAATTY 3 cut(s) 548, 650, 1010
AfaI GTAC 4 cut(s) 122, 397, 600, 828
AfiI CCNNNNNNNGG 1 cut(s) 707
AflII CTTAAG 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 8 cut(s) 29, 313, 334, 594, 778, 853, 962, 1133
AhdI GACNNNNNGTC 1 cut(s) 279
AjiI CACGTC 1 cut(s) 116
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 8 cut(s) 309, 344, 352, 380, 584, 892, 1043, 1067
AluI AGCT 8 cut(s) 309, 344, 352, 380, 584, 892, 1043, 1067
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 2 cut(s) 992, 1097
Aor13HI TCCGGA 1 cut(s) 1000
ApeKI GCWGC 2 cut(s) 527, 1145
ApoI RAATTY 3 cut(s) 548, 650, 1010
AspLEI GCGC 2 cut(s) 485, 766
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 475
AsuII TTCGAA 1 cut(s) 145
AvaII GGWCC 1 cut(s) 470
BarI GAAGNNNNNNTAC 2 cut(s) 37, 69
BbsI GAAGAC 2 cut(s) 52, 102
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 514, 1157
BccI CCATC 3 cut(s) 14, 56, 434
BcgI CGANNNNNNTGC 4 cut(s) 135, 169, 233, 267
BciT130I CCWGG 1 cut(s) 645
BciVI GTATCC 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 2 cut(s) 102, 893
BfmI CTRYAG 1 cut(s) 57
BfrI CTTAAG 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 1102
BfuI GTATCC 1 cut(s) 59
BisI GCNGC 2 cut(s) 528, 1146
BlsI GCNGC 2 cut(s) 529, 1147
BmcAI AGTACT 2 cut(s) 600, 828
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 470
BmeRI GACNNNNNGTC 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 5 cut(s) 213, 598, 655, 720, 1075
BpiI GAAGAC 2 cut(s) 52, 102
BpmI CTGGAG 2 cut(s) 330, 627
Bpu14I TTCGAA 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 817
Bsa29I ATCGAT 1 cut(s) 253
BsaWI WCCGGW 2 cut(s) 964, 1000
BsaXI ACNNNNNCTCC 6 cut(s) 328, 358, 847, 877, 881, 911
Bsc4I CCNNNNNNNGG 1 cut(s) 707
Bse1I ACTGG 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 1000
BseBI CCWGG 1 cut(s) 645
BseCI ATCGAT 1 cut(s) 253
BseGI GGATG 4 cut(s) 6, 670, 711, 976
BseLI CCNNNNNNNGG 1 cut(s) 707
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 2 cut(s) 465, 577
BseNI ACTGG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 514, 1157
BsgI GTGCAG 3 cut(s) 843, 927, 1080
Bsh1236I CGCG 1 cut(s) 766
BshVI ATCGAT 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 564
BsiSI CCGG 2 cut(s) 965, 1001
BslFI GGGAC 2 cut(s) 457, 483
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 457, 483
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 564
Bsp13I TCCGGA 1 cut(s) 1000
Bsp1407I TGTACA 1 cut(s) 120
Bsp143I GATC 3 cut(s) 732, 997, 1102
BspACI CCGC 2 cut(s) 715, 887
BspCNI CTCAG 2 cut(s) 466, 576
BspDI ATCGAT 1 cut(s) 253
BspEI TCCGGA 1 cut(s) 1000
BspFNI CGCG 1 cut(s) 766
BspHI TCATGA 2 cut(s) 586, 1157
BspLI GGNNCC 1 cut(s) 471
BspMI ACCTGC 1 cut(s) 1102
BspPI GGATC 2 cut(s) 992, 1097
BspT104I TTCGAA 1 cut(s) 145
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 465
BsrGI TGTACA 1 cut(s) 120
BsrI ACTGG 1 cut(s) 50
BssMI GATC 3 cut(s) 732, 997, 1102
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 1 cut(s) 425
BstAFI CTTAAG 1 cut(s) 632
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstAUI TGTACA 1 cut(s) 120
BstBI TTCGAA 1 cut(s) 145
BstC8I GCNNGC 3 cut(s) 219, 264, 378
BstDEI CTNAG 3 cut(s) 474, 563, 694
BstENI CCTNNNNNAGG 1 cut(s) 705
BstF5I GGATG 4 cut(s) 6, 670, 711, 976
BstFNI CGCG 1 cut(s) 766
BstHHI GCGC 2 cut(s) 485, 766
BstKTI GATC 3 cut(s) 735, 1000, 1105
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 3 cut(s) 732, 997, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 674, 1094
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 643
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 2 cut(s) 514, 1157
BstV2I GAAGAC 2 cut(s) 52, 102
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
Bsu15I ATCGAT 1 cut(s) 253
BsuI GTATCC 1 cut(s) 59
BsuTUI ATCGAT 1 cut(s) 253
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 4 cut(s) 6, 670, 711, 976
BtsIMutI CAGTG 2 cut(s) 571, 742
BveI ACCTGC 1 cut(s) 1102
Cac8I GCNNGC 3 cut(s) 219, 264, 378
CciI TCATGA 2 cut(s) 586, 1157
CfoI GCGC 2 cut(s) 485, 766
Cfr13I GGNCC 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 253
Csp6I GTAC 4 cut(s) 121, 396, 599, 827
CviAII CATG 4 cut(s) 587, 686, 788, 1158
CviQI GTAC 4 cut(s) 121, 396, 599, 827
DdeI CTNAG 3 cut(s) 474, 563, 694
DpnI GATC 3 cut(s) 734, 999, 1104
DpnII GATC 3 cut(s) 732, 997, 1102
DriI GACNNNNNGTC 1 cut(s) 279
Eam1105I GACNNNNNGTC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 470
EcoNI CCTNNNNNAGG 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 548, 1010
EcoRII CCWGG 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 613
FaeI CATG 4 cut(s) 590, 689, 791, 1161
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 2 cut(s) 457, 483
FatI CATG 4 cut(s) 586, 685, 787, 1157
FauNDI CATATG 1 cut(s) 1074
Fnu4HI GCNGC 2 cut(s) 528, 1146
FokI GGATG 3 cut(s) 677, 698, 983
Fsp4HI GCNGC 2 cut(s) 528, 1146
FspBI CTAG 2 cut(s) 102, 893
GlaI GCGC 2 cut(s) 484, 765
GluI GCNGC 2 cut(s) 528, 1146
GsuI CTGGAG 2 cut(s) 330, 627
HapII CCGG 2 cut(s) 965, 1001
HhaI GCGC 2 cut(s) 485, 766
Hin1II CATG 4 cut(s) 590, 689, 791, 1161
Hin6I GCGC 2 cut(s) 483, 764
HinP1I GCGC 2 cut(s) 483, 764
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 1 cut(s) 590
HpaII CCGG 2 cut(s) 965, 1001
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 2 cut(s) 35, 121
Hpy188I TCNGA 2 cut(s) 167, 946
Hpy8I GTNNAC 2 cut(s) 35, 121
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 3 cut(s) 460, 569, 1127
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 5 cut(s) 22, 31, 115, 338, 804
HpyF10VI GCNNNNNNNGC 2 cut(s) 674, 1094
HpyF3I CTNAG 3 cut(s) 474, 563, 694
HpySE526I ACGT 5 cut(s) 22, 31, 115, 338, 804
Hsp92II CATG 4 cut(s) 590, 689, 791, 1161
HspAI GCGC 2 cut(s) 483, 764
Kpn2I TCCGGA 1 cut(s) 1000
Kzo9I GATC 3 cut(s) 732, 997, 1102
LmnI GCTCC 3 cut(s) 349, 431, 889
Lsp1109I GCAGC 2 cut(s) 514, 1157
LweI GCATC 5 cut(s) 213, 598, 655, 720, 1075
MaeI CTAG 2 cut(s) 102, 893
MaeII ACGT 5 cut(s) 22, 31, 115, 338, 804
MaeIII GTNAC 2 cut(s) 52, 636
MalI GATC 3 cut(s) 734, 999, 1104
MboI GATC 3 cut(s) 732, 997, 1102
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 5 cut(s) 222, 435, 548, 650, 1010
MnlI CCTC 6 cut(s) 651, 701, 873, 961, 1030, 1128
Mph1103I ATGCAT 1 cut(s) 613
MroI TCCGGA 1 cut(s) 1000
MseI TTAA 5 cut(s) 324, 417, 633, 831, 876
MslI CAYNNNNRTG 1 cut(s) 557
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 2 cut(s) 965, 1001
MspR9I CCNGG 1 cut(s) 645
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 2 cut(s) 674, 1094
NdeI CATATG 1 cut(s) 1074
NdeII GATC 3 cut(s) 732, 997, 1102
NlaIII CATG 4 cut(s) 590, 689, 791, 1161
NlaIV GGNNCC 1 cut(s) 471
NsiI ATGCAT 1 cut(s) 613
NspV TTCGAA 1 cut(s) 145
PagI TCATGA 2 cut(s) 586, 1157
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PfeI GAWTC 1 cut(s) 590
PkrI GCNGC 2 cut(s) 529, 1147
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 470
PspPPI RGGWCCY 1 cut(s) 470
PsuI RGATCY 1 cut(s) 997
RsaI GTAC 4 cut(s) 122, 397, 600, 828
RsaNI GTAC 4 cut(s) 121, 396, 599, 827
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 5 cut(s) 324, 417, 633, 831, 876
SatI GCNGC 2 cut(s) 528, 1146
Sau3AI GATC 3 cut(s) 732, 997, 1102
Sau96I GGNCC 1 cut(s) 470
ScaI AGTACT 2 cut(s) 600, 828
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 1 cut(s) 564
SfaNI GCATC 5 cut(s) 213, 598, 655, 720, 1075
SfcI CTRYAG 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 632, 796
SmoI CTYRAG 2 cut(s) 632, 796
Sse9I AATT 5 cut(s) 222, 435, 548, 650, 1010
SsiI CCGC 2 cut(s) 715, 887
SspI AATATT 2 cut(s) 819, 849
SspMI CTAG 2 cut(s) 102, 893
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 5 cut(s) 25, 34, 118, 341, 807
TaqI TCGA 5 cut(s) 43, 145, 184, 207, 253
TasI AATT 5 cut(s) 222, 435, 548, 650, 1010
TatI WGTACW 4 cut(s) 120, 395, 598, 826
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 5 cut(s) 324, 417, 633, 831, 876
Tru9I TTAA 5 cut(s) 324, 417, 633, 831, 876
TscAI CASTG 2 cut(s) 571, 742
TseI GCWGC 2 cut(s) 527, 1145
TspDTI ATGAA 6 cut(s) 108, 541, 987, 999, 1005, 1023
TspRI CASTG 2 cut(s) 571, 742
Vha464I CTTAAG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 705
XapI RAATTY 3 cut(s) 548, 650, 1010
XspI CTAG 2 cut(s) 102, 893
ZrmI AGTACT 2 cut(s) 600, 828
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.