nuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 145, 799 |
| AccBSI | CCGCTC | 1 cut(s) | 695 |
| AccI | GTMKAC | 1 cut(s) | 157 |
| AciI | CCGC | 3 cut(s) | 146, 693, 780 |
| AcoI | YGGCCR | 1 cut(s) | 352 |
| AcyI | GRCGYC | 2 cut(s) | 142, 796 |
| AfaI | GTAC | 2 cut(s) | 508, 823 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 818 |
| AflIII | ACRYGT | 2 cut(s) | 15, 249 |
| AgsI | TTSAA | 8 cut(s) | 118, 320, 424, 436, 445, 705, 715, 881 |
| AhlI | ACTAGT | 1 cut(s) | 343 |
| AjiI | CACGTC | 2 cut(s) | 250, 584 |
| AjnI | CCWGG | 1 cut(s) | 754 |
| AleI | CACNNNNGTG | 1 cut(s) | 312 |
| AluBI | AGCT | 5 cut(s) | 26, 377, 545, 641, 809 |
| AluI | AGCT | 5 cut(s) | 26, 377, 545, 641, 809 |
| Alw26I | GTCTC | 1 cut(s) | 201 |
| AoxI | GGCC | 2 cut(s) | 352, 438 |
| ApeKI | GCWGC | 1 cut(s) | 638 |
| AseI | ATTAAT | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 3 cut(s) | 140, 824, 863 |
| AsuII | TTCGAA | 1 cut(s) | 256 |
| BbvI | GCAGC | 1 cut(s) | 650 |
| BccI | CCATC | 2 cut(s) | 545, 675 |
| BciT130I | CCWGG | 1 cut(s) | 756 |
| BciVI | GTATCC | 3 cut(s) | 170, 252, 292 |
| BcoDI | GTCTC | 1 cut(s) | 201 |
| BcuI | ACTAGT | 1 cut(s) | 343 |
| BfaI | CTAG | 3 cut(s) | 344, 729, 806 |
| BfmI | CTRYAG | 1 cut(s) | 546 |
| BfuI | GTATCC | 3 cut(s) | 170, 252, 292 |
| BisI | GCNGC | 1 cut(s) | 639 |
| BlsI | GCNGC | 1 cut(s) | 640 |
| Bme1390I | CCNGG | 1 cut(s) | 756 |
| BmgBI | CACGTC | 2 cut(s) | 250, 584 |
| BmiI | GGNNCC | 2 cut(s) | 691, 803 |
| BmrFI | CCNGG | 1 cut(s) | 756 |
| BmsI | GCATC | 2 cut(s) | 279, 709 |
| Bpu14I | TTCGAA | 1 cut(s) | 256 |
| Bsa29I | ATCGAT | 1 cut(s) | 364 |
| BsaAI | YACGTR | 1 cut(s) | 125 |
| BsaHI | GRCGYC | 2 cut(s) | 142, 796 |
| BsaI | GGTCTC | 1 cut(s) | 201 |
| BsaJI | CCNNGG | 1 cut(s) | 610 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 818 |
| Bse1I | ACTGG | 1 cut(s) | 161 |
| BseBI | CCWGG | 1 cut(s) | 756 |
| BseCI | ATCGAT | 1 cut(s) | 364 |
| BseDI | CCNNGG | 1 cut(s) | 610 |
| BseGI | GGATG | 2 cut(s) | 270, 686 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 818 |
| BseMII | CTCAG | 1 cut(s) | 468 |
| BseNI | ACTGG | 1 cut(s) | 161 |
| BseXI | GCAGC | 1 cut(s) | 650 |
| BshFI | GGCC | 2 cut(s) | 354, 440 |
| BshVI | ATCGAT | 1 cut(s) | 364 |
| BslFI | GGGAC | 1 cut(s) | 788 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 818 |
| BsmAI | GTCTC | 1 cut(s) | 201 |
| BsmFI | GGGAC | 1 cut(s) | 788 |
| BsnI | GGCC | 2 cut(s) | 354, 440 |
| Bso31I | GGTCTC | 1 cut(s) | 201 |
| Bsp119I | TTCGAA | 1 cut(s) | 256 |
| Bsp143I | GATC | 1 cut(s) | 395 |
| Bsp19I | CCATGG | 1 cut(s) | 610 |
| BspACI | CCGC | 3 cut(s) | 146, 693, 780 |
| BspANI | GGCC | 2 cut(s) | 354, 440 |
| BspCNI | CTCAG | 1 cut(s) | 469 |
| BspDI | ATCGAT | 1 cut(s) | 364 |
| BspHI | TCATGA | 1 cut(s) | 697 |
| BspLI | GGNNCC | 2 cut(s) | 691, 803 |
| BspQI | GCTCTTC | 1 cut(s) | 33 |
| BspT104I | TTCGAA | 1 cut(s) | 256 |
| BspTNI | GGTCTC | 1 cut(s) | 201 |
| BsrBI | CCGCTC | 1 cut(s) | 695 |
| BsrI | ACTGG | 1 cut(s) | 161 |
| BssECI | CCNNGG | 1 cut(s) | 610 |
| BssMI | GATC | 1 cut(s) | 395 |
| BssNI | GRCGYC | 2 cut(s) | 142, 796 |
| BssT1I | CCWWGG | 1 cut(s) | 610 |
| Bst2UI | CCWGG | 1 cut(s) | 756 |
| Bst4CI | ACNGT | 3 cut(s) | 462, 574, 660 |
| Bst6I | CTCTTC | 2 cut(s) | 27, 33 |
| BstACI | GRCGYC | 2 cut(s) | 142, 796 |
| BstBAI | YACGTR | 1 cut(s) | 125 |
| BstBI | TTCGAA | 1 cut(s) | 256 |
| BstC8I | GCNNGC | 2 cut(s) | 330, 557 |
| BstDEI | CTNAG | 2 cut(s) | 477, 769 |
| BstDSI | CCRYGG | 1 cut(s) | 610 |
| BstF5I | GGATG | 2 cut(s) | 270, 686 |
| BstKTI | GATC | 1 cut(s) | 398 |
| BstMAI | GTCTC | 1 cut(s) | 201 |
| BstMBI | GATC | 1 cut(s) | 395 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 83 |
| BstNI | CCWGG | 1 cut(s) | 756 |
| BstNSI | RCATGY | 1 cut(s) | 19 |
| BstSCI | CCNGG | 1 cut(s) | 754 |
| BstSFI | CTRYAG | 1 cut(s) | 546 |
| BstV1I | GCAGC | 1 cut(s) | 650 |
| Bsu15I | ATCGAT | 1 cut(s) | 364 |
| BsuI | GTATCC | 3 cut(s) | 170, 252, 292 |
| BsuRI | GGCC | 2 cut(s) | 354, 440 |
| BsuTUI | ATCGAT | 1 cut(s) | 364 |
| BtgI | CCRYGG | 1 cut(s) | 610 |
| BtrI | CACGTC | 2 cut(s) | 250, 584 |
| BtsCI | GGATG | 2 cut(s) | 270, 686 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 2 cut(s) | 93, 168 |
| Cac8I | GCNNGC | 2 cut(s) | 330, 557 |
| CciI | TCATGA | 1 cut(s) | 697 |
| ClaI | ATCGAT | 1 cut(s) | 364 |
| CsiI | ACCWGGT | 1 cut(s) | 754 |
| Csp6I | GTAC | 2 cut(s) | 507, 822 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 106, 141 |
| CviAII | CATG | 4 cut(s) | 16, 611, 698, 891 |
| CviQI | GTAC | 2 cut(s) | 507, 822 |
| DdeI | CTNAG | 2 cut(s) | 477, 769 |
| DpnI | GATC | 1 cut(s) | 397 |
| DpnII | GATC | 1 cut(s) | 395 |
| EaeI | YGGCCR | 1 cut(s) | 352 |
| Eam1104I | CTCTTC | 2 cut(s) | 27, 33 |
| EarI | CTCTTC | 2 cut(s) | 27, 33 |
| Eco130I | CCWWGG | 1 cut(s) | 610 |
| Eco147I | AGGCCT | 1 cut(s) | 440 |
| Eco31I | GGTCTC | 1 cut(s) | 201 |
| Eco32I | GATATC | 3 cut(s) | 599, 621, 830 |
| EcoRII | CCWGG | 1 cut(s) | 754 |
| EcoRV | GATATC | 3 cut(s) | 599, 621, 830 |
| EcoT14I | CCWWGG | 1 cut(s) | 610 |
| ErhI | CCWWGG | 1 cut(s) | 610 |
| FaeI | CATG | 4 cut(s) | 19, 614, 701, 894 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 312, 344 |
| FaqI | GGGAC | 1 cut(s) | 788 |
| FatI | CATG | 4 cut(s) | 15, 610, 697, 890 |
| FblI | GTMKAC | 1 cut(s) | 157 |
| Fnu4HI | GCNGC | 1 cut(s) | 639 |
| FokI | GGATG | 2 cut(s) | 257, 693 |
| Fsp4HI | GCNGC | 1 cut(s) | 639 |
| FspBI | CTAG | 3 cut(s) | 344, 729, 806 |
| GluI | GCNGC | 1 cut(s) | 639 |
| HaeIII | GGCC | 2 cut(s) | 354, 440 |
| Hin1I | GRCGYC | 2 cut(s) | 142, 796 |
| Hin1II | CATG | 4 cut(s) | 19, 614, 701, 894 |
| HincII | GTYRAC | 1 cut(s) | 158 |
| HindII | GTYRAC | 1 cut(s) | 158 |
| HinfI | GANTC | 5 cut(s) | 151, 479, 701, 741, 783 |
| HphI | GGTGA | 3 cut(s) | 140, 824, 863 |
| Hpy166II | GTNNAC | 2 cut(s) | 131, 158 |
| Hpy188I | TCNGA | 2 cut(s) | 7, 542 |
| Hpy188III | TCNNGA | 4 cut(s) | 245, 433, 698, 729 |
| Hpy8I | GTNNAC | 2 cut(s) | 131, 158 |
| Hpy99I | CGWCG | 3 cut(s) | 159, 798, 801 |
| HpyAV | CCTTC | 2 cut(s) | 430, 848 |
| HpyCH4III | ACNGT | 3 cut(s) | 462, 574, 660 |
| HpyCH4IV | ACGT | 6 cut(s) | 124, 142, 249, 583, 796, 907 |
| HpyCH4V | TGCA | 4 cut(s) | 190, 332, 569, 875 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 83 |
| HpyF3I | CTNAG | 2 cut(s) | 477, 769 |
| HpySE526I | ACGT | 6 cut(s) | 124, 142, 249, 583, 796, 907 |
| Hsp92I | GRCGYC | 2 cut(s) | 142, 796 |
| Hsp92II | CATG | 4 cut(s) | 19, 614, 701, 894 |
| Kzo9I | GATC | 1 cut(s) | 395 |
| LguI | GCTCTTC | 1 cut(s) | 33 |
| LmnI | GCTCC | 3 cut(s) | 23, 542, 814 |
| LpnPI | CCDG | 6 cut(s) | 174, 258, 489, 579, 741, 768 |
| Lsp1109I | GCAGC | 1 cut(s) | 650 |
| LweI | GCATC | 2 cut(s) | 279, 709 |
| MabI | ACCWGGT | 1 cut(s) | 754 |
| MaeI | CTAG | 3 cut(s) | 344, 729, 806 |
| MaeII | ACGT | 6 cut(s) | 124, 142, 249, 583, 796, 907 |
| MaeIII | GTNAC | 1 cut(s) | 163 |
| MalI | GATC | 1 cut(s) | 397 |
| MbiI | CCGCTC | 1 cut(s) | 695 |
| MboI | GATC | 1 cut(s) | 395 |
| MboII | GAAGA | 5 cut(s) | 44, 50, 332, 628, 876 |
| MluCI | AATT | 3 cut(s) | 260, 876, 910 |
| MlyI | GAGTC | 3 cut(s) | 145, 488, 735 |
| MmeI | TCCRAC | 2 cut(s) | 520, 559 |
| MnlI | CCTC | 8 cut(s) | 28, 46, 222, 580, 679, 812, 845, 895 |
| MseI | TTAA | 4 cut(s) | 60, 71, 528, 645 |
| MslI | CAYNNNNRTG | 3 cut(s) | 185, 312, 668 |
| MspA1I | CMGCKG | 1 cut(s) | 148 |
| MspR9I | CCNGG | 1 cut(s) | 756 |
| MvaI | CCWGG | 1 cut(s) | 756 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 83 |
| NcoI | CCATGG | 1 cut(s) | 610 |
| NdeII | GATC | 1 cut(s) | 395 |
| NlaIII | CATG | 4 cut(s) | 19, 614, 701, 894 |
| NlaIV | GGNNCC | 2 cut(s) | 691, 803 |
| NmeAIII | GCCGAG | 1 cut(s) | 358 |
| NmuCI | GTSAC | 1 cut(s) | 163 |
| NspI | RCATGY | 1 cut(s) | 19 |
| NspV | TTCGAA | 1 cut(s) | 256 |
| OliI | CACNNNNGTG | 1 cut(s) | 312 |
| PagI | TCATGA | 1 cut(s) | 697 |
| PceI | AGGCCT | 1 cut(s) | 440 |
| PciI | ACATGT | 1 cut(s) | 15 |
| PciSI | GCTCTTC | 1 cut(s) | 33 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 823 |
| PfeI | GAWTC | 2 cut(s) | 701, 783 |
| PkrI | GCNGC | 1 cut(s) | 640 |
| PleI | GAGTC | 3 cut(s) | 145, 487, 735 |
| PpsI | GAGTC | 3 cut(s) | 145, 487, 735 |
| Ppu21I | YACGTR | 1 cut(s) | 125 |
| PscI | ACATGT | 1 cut(s) | 15 |
| PshBI | ATTAAT | 1 cut(s) | 71 |
| Psp6I | CCWGG | 1 cut(s) | 754 |
| PspGI | CCWGG | 1 cut(s) | 754 |
| PspN4I | GGNNCC | 2 cut(s) | 691, 803 |
| RsaI | GTAC | 2 cut(s) | 508, 823 |
| RsaNI | GTAC | 2 cut(s) | 507, 822 |
| RseI | CAYNNNNRTG | 3 cut(s) | 185, 312, 668 |
| SalI | GTCGAC | 1 cut(s) | 156 |
| SapI | GCTCTTC | 1 cut(s) | 33 |
| SaqAI | TTAA | 4 cut(s) | 60, 71, 528, 645 |
| SatI | GCNGC | 1 cut(s) | 639 |
| Sau3AI | GATC | 1 cut(s) | 395 |
| SchI | GAGTC | 3 cut(s) | 145, 488, 735 |
| ScrFI | CCNGG | 1 cut(s) | 756 |
| SexAI | ACCWGGT | 1 cut(s) | 754 |
| SfaNI | GCATC | 2 cut(s) | 279, 709 |
| SfcI | CTRYAG | 1 cut(s) | 546 |
| SfuI | TTCGAA | 1 cut(s) | 256 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 185, 312, 668 |
| SmlI | CTYRAG | 1 cut(s) | 899 |
| SmoI | CTYRAG | 1 cut(s) | 899 |
| SpeI | ACTAGT | 1 cut(s) | 343 |
| Sse9I | AATT | 3 cut(s) | 260, 876, 910 |
| SseBI | AGGCCT | 1 cut(s) | 440 |
| SsiI | CCGC | 3 cut(s) | 146, 693, 780 |
| SspI | AATATT | 1 cut(s) | 764 |
| SspMI | CTAG | 3 cut(s) | 344, 729, 806 |
| StuI | AGGCCT | 1 cut(s) | 440 |
| StyD4I | CCNGG | 1 cut(s) | 754 |
| StyI | CCWWGG | 1 cut(s) | 610 |
| TaaI | ACNGT | 3 cut(s) | 462, 574, 660 |
| TaiI | ACGT | 6 cut(s) | 127, 145, 252, 586, 799, 910 |
| TaqI | TCGA | 7 cut(s) | 157, 256, 364, 601, 793, 817, 826 |
| TasI | AATT | 3 cut(s) | 260, 876, 910 |
| TatI | WGTACW | 1 cut(s) | 506 |
| TfiI | GAWTC | 2 cut(s) | 701, 783 |
| Tru1I | TTAA | 4 cut(s) | 60, 71, 528, 645 |
| Tru9I | TTAA | 4 cut(s) | 60, 71, 528, 645 |
| TscAI | CASTG | 2 cut(s) | 93, 168 |
| TseFI | GTSAC | 1 cut(s) | 163 |
| TseI | GCWGC | 1 cut(s) | 638 |
| Tsp45I | GTSAC | 1 cut(s) | 163 |
| TspDTI | ATGAA | 3 cut(s) | 45, 387, 652 |
| TspRI | CASTG | 2 cut(s) | 93, 168 |
| VspI | ATTAAT | 1 cut(s) | 71 |
| XbaI | TCTAGA | 1 cut(s) | 728 |
| XceI | RCATGY | 1 cut(s) | 19 |
| XmiI | GTMKAC | 1 cut(s) | 157 |
| XspI | CTAG | 3 cut(s) | 344, 729, 806 |
| ZraI | GACGTC | 2 cut(s) | 143, 797 |