Rmu_sc0000029.1_g000014

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000029.1
OG0000697
Physical Location & Seq
Reverse (-)
92959 .. 94437
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000029.1_g000014.1.cds

Sequence Viewer

Length: 1080 bp
atgtctgaaatggacatgtatggagctgacgaagtggaagagcagttttacgaggctgttaagatattattaatggcaatacaagcagtgatttatgtgttatacgaccttgtgtttagcatacgtggtgaacatattagacgtccgctgactcgtagaccagtgacatcaagtggatacatatatatatgcacaacatattggacagagaccctcaaatctttagagagaagtttcaacaaagtcttgaaggccttgaatacaatagcaccacagtttatggctaaacctgagtccataccacctaacataagagaaagtacaaggttctatccttactttaaggattgtgtcggagctatagatggcacgtatattccagcaacggtagttggacgtgaggtaagcagatatcgaaatcgccatgggacgatatcacaaaacgtattagcagcttgtaactttgatttacagttcatatatgtgattagtggatgggagggttccgctcatgattcaaaagtgttgaatgatgcgatttctagacgaaatggactcaaagtgccaccaggtaaatattacttagggaactgcggattcccaaatcgacgtctgtttctagctccatttcgaggtactcgatatcacctcaaagattttggtggtgaaggaaaccatcctgtcaatgcaattgagttattcaatcttcgccatgcttccttgaggaacgtaattgagagaatatttggaatatttaagtcacgtttcacaatcttcaaatcagcaccaccatttctatataagacacaagcagaactagttttggcttgtgcaggactgcacaattttcttcgacaggaatgtcgttcaaatgaatttcctcctaaaccagaagaagatccgatagacaatcacgaagataattttgaatgggatgattttcaaacccaagatcagcaaagagagaatgctaatgaatggggaatgagtattgctactcatatgtggacagatgcccaaccaaatgccaacaatgaaaacaatgacaatcaagaaagtgaaaatgaaggagaagaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

359

Amino Acids

41.37

Weight (kDa)

5.69

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 861
AatII GACGTC 2 cut(s) 145, 613
AccBSI CCGCTC 1 cut(s) 509
AccI GTMKAC 1 cut(s) 157
AciI CCGC 3 cut(s) 146, 507, 594
AclWI GGATC 1 cut(s) 893
AcsI RAATTY 1 cut(s) 875
AcyI GRCGYC 2 cut(s) 142, 610
AfaI GTAC 2 cut(s) 322, 637
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 15
AhlI ACTAGT 1 cut(s) 817
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 1 cut(s) 568
AluBI AGCT 4 cut(s) 26, 359, 455, 623
AluI AGCT 4 cut(s) 26, 359, 455, 623
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 893
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 875
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 140, 638, 677
BbvI GCAGC 1 cut(s) 464
BccI CCATC 3 cut(s) 359, 489, 684
BciT130I CCWGG 1 cut(s) 570
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 203
BcuI ACTAGT 1 cut(s) 817
BfaI CTAG 3 cut(s) 543, 620, 818
BfmI CTRYAG 1 cut(s) 360
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 570
BmgBI CACGTC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 1 cut(s) 570
BmsI GCATC 2 cut(s) 523, 1003
BpuEI CTTGAG 1 cut(s) 742
BsaAI YACGTR 2 cut(s) 125, 372
BsaHI GRCGYC 2 cut(s) 142, 610
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse1I ACTGG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 3 cut(s) 500, 676, 940
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 464
BsgI GTGCAG 2 cut(s) 824, 852
BshFI GGCC 1 cut(s) 254
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 632
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 442
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 1 cut(s) 254
Bso31I GGTCTC 1 cut(s) 203
Bsp143I GATC 2 cut(s) 898, 952
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 3 cut(s) 146, 507, 594
BspANI GGCC 1 cut(s) 254
BspCNI CTCAG 1 cut(s) 283
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 505
BspPI GGATC 1 cut(s) 893
BspQI GCTCTTC 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 509
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 2 cut(s) 898, 952
BssNI GRCGYC 2 cut(s) 142, 610
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 3 cut(s) 276, 388, 474
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 2 cut(s) 142, 610
BstBAI YACGTR 2 cut(s) 125, 372
BstDEI CTNAG 2 cut(s) 291, 583
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 3 cut(s) 500, 676, 940
BstKTI GATC 2 cut(s) 901, 955
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 2 cut(s) 898, 952
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 1 cut(s) 568
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 1 cut(s) 424
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 3 cut(s) 500, 676, 940
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 168
CciI TCATGA 1 cut(s) 511
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 2 cut(s) 321, 636
CviAII CATG 4 cut(s) 16, 425, 512, 713
CviJI RGCY 8 cut(s) 26, 56, 254, 284, 359, 455, 623, 827
CviKI_1 RGCY 8 cut(s) 26, 56, 254, 284, 359, 455, 623, 827
CviQI GTAC 2 cut(s) 321, 636
DdeI CTNAG 2 cut(s) 291, 583
DpnI GATC 2 cut(s) 900, 954
DpnII GATC 2 cut(s) 898, 952
DrdI GACNNNNNNGTC 1 cut(s) 861
DseDI GACNNNNNNGTC 1 cut(s) 861
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 254
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 3 cut(s) 413, 435, 644
EcoRII CCWGG 1 cut(s) 568
EcoRV GATATC 3 cut(s) 413, 435, 644
EcoT14I CCWWGG 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 4 cut(s) 19, 428, 515, 716
FaqI GGGAC 1 cut(s) 442
FatI CATG 4 cut(s) 15, 424, 511, 712
FauNDI CATATG 1 cut(s) 1002
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 3 cut(s) 507, 663, 947
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 3 cut(s) 543, 620, 818
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 1 cut(s) 254
Hin1I GRCGYC 2 cut(s) 142, 610
Hin1II CATG 4 cut(s) 19, 428, 515, 716
HinfI GANTC 5 cut(s) 151, 293, 515, 555, 597
HphI GGTGA 3 cut(s) 140, 638, 677
Hpy166II GTNNAC 3 cut(s) 131, 158, 1008
Hpy188I TCNGA 3 cut(s) 7, 356, 903
Hpy188III TCNNGA 5 cut(s) 247, 512, 543, 914, 1052
Hpy8I GTNNAC 3 cut(s) 131, 158, 1008
Hpy99I CGWCG 1 cut(s) 612
HpyAV CCTTC 3 cut(s) 244, 662, 1061
HpyCH4III ACNGT 3 cut(s) 276, 388, 474
HpyCH4IV ACGT 8 cut(s) 124, 142, 371, 397, 444, 610, 729, 763
HpyCH4V TGCA 4 cut(s) 192, 689, 833, 841
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 291, 583
HpySE526I ACGT 8 cut(s) 124, 142, 371, 397, 444, 610, 729, 763
Hsp92I GRCGYC 2 cut(s) 142, 610
Hsp92II CATG 4 cut(s) 19, 428, 515, 716
Kzo9I GATC 2 cut(s) 898, 952
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 3 cut(s) 23, 356, 628
LpnPI CCDG 9 cut(s) 174, 303, 393, 555, 582, 693, 819, 842, 903
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 2 cut(s) 523, 1003
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 3 cut(s) 543, 620, 818
MaeII ACGT 8 cut(s) 124, 142, 371, 397, 444, 610, 729, 763
MaeIII GTNAC 3 cut(s) 163, 458, 759
MalI GATC 2 cut(s) 900, 954
MbiI CCGCTC 1 cut(s) 509
MboI GATC 2 cut(s) 898, 952
MboII GAAGA 7 cut(s) 50, 698, 766, 842, 905, 908, 929
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 1 cut(s) 898
MluCI AATT 5 cut(s) 690, 732, 844, 875, 922
MlyI GAGTC 3 cut(s) 145, 302, 549
MmeI TCCRAC 2 cut(s) 334, 373
MnlI CCTC 8 cut(s) 46, 224, 394, 493, 626, 659, 717, 891
MseI TTAA 4 cut(s) 60, 71, 342, 756
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 570
MunI CAATTG 1 cut(s) 690
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 424
NdeI CATATG 1 cut(s) 1002
NdeII GATC 2 cut(s) 898, 952
NlaIII CATG 4 cut(s) 19, 428, 515, 716
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 163, 759
NspI RCATGY 1 cut(s) 19
PagI TCATGA 1 cut(s) 511
PceI AGGCCT 1 cut(s) 254
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 637
PctI GAATGC 1 cut(s) 973
PfeI GAWTC 2 cut(s) 515, 597
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 3 cut(s) 145, 301, 549
PpsI GAGTC 3 cut(s) 145, 301, 549
Ppu21I YACGTR 2 cut(s) 125, 372
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 1 cut(s) 505
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 2 cut(s) 322, 637
RsaNI GTAC 2 cut(s) 321, 636
RseI CAYNNNNRTG 1 cut(s) 482
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 4 cut(s) 60, 71, 342, 756
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 2 cut(s) 898, 952
SchI GAGTC 3 cut(s) 145, 302, 549
ScrFI CCNGG 1 cut(s) 570
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 2 cut(s) 523, 1003
SfcI CTRYAG 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 721
SmoI CTYRAG 1 cut(s) 721
SpeI ACTAGT 1 cut(s) 817
Sse9I AATT 5 cut(s) 690, 732, 844, 875, 922
SseBI AGGCCT 1 cut(s) 254
SsiI CCGC 3 cut(s) 146, 507, 594
SspI AATATT 3 cut(s) 578, 744, 753
SspMI CTAG 3 cut(s) 543, 620, 818
StuI AGGCCT 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 276, 388, 474
TaiI ACGT 8 cut(s) 127, 145, 374, 400, 447, 613, 732, 766
TaqI TCGA 5 cut(s) 415, 607, 631, 640, 853
TasI AATT 5 cut(s) 690, 732, 844, 875, 922
TatI WGTACW 1 cut(s) 320
TfiI GAWTC 2 cut(s) 515, 597
Tru1I TTAA 4 cut(s) 60, 71, 342, 756
Tru9I TTAA 4 cut(s) 60, 71, 342, 756
TscAI CASTG 2 cut(s) 93, 168
TseFI GTSAC 2 cut(s) 163, 759
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 2 cut(s) 163, 759
TspDTI ATGAA 5 cut(s) 466, 888, 990, 1050, 1080
TspRI CASTG 2 cut(s) 93, 168
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 875
XbaI TCTAGA 1 cut(s) 542
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 3 cut(s) 543, 620, 818
ZraI GACGTC 2 cut(s) 143, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.