Rmu_sc0000913.1_g000001

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000913.1
OG0000697
Physical Location & Seq
Forward (+)
1 .. 2124
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000913.1_g000001.1.cds

Sequence Viewer

Length: 735 bp
gattgtgtcggagctatagatggcacacatattccagcaacggtagttggacgtgaggttagcagatatcgaaatcgccatgggaagatatcacaaaatgtattagcagcttgtaactttgatttacagttcacatatgtaattagtggatgggagggttccgctcatgattcaaaagtattgaatgatgcgatttctagacgaaatggactcaaagtgccaccaggtaaatattacttaggggactgcagatttccaaatcgacgccggtttttagctccatttcgaggtactcgatatcatctcaaagattttagtggtgaaggaaaccatcctgtcaatgcaattgagttattcaatcttcgtcatgcttccttgaggaatgtaattgagagaatgtttggaatatttaagtcgcgtttcacaatcttcaaatcagcaccaccattttcatataagacacaagcagagctagttttggcttgtgcaggactgcacaattttcttcgacaggaatgccgttcagatgaatttcctcccgaaccagaagaagatccgatagacaatcacgaagataattttgaatgggatgattttcaaacccaagatcagcagagagaaaatgctaatgaatggagaatgagtattgctactcatatgtggacaaatgcccaaccaaatgccaacaatgaaaacaataacaatgaagaaagtgaaaatgaaggagaagaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

244

Amino Acids

28.11

Weight (kDa)

5.64

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 418
AciI CCGC 1 cut(s) 162
AclWI GGATC 1 cut(s) 548
AcsI RAATTY 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 6 cut(s) 174, 184, 358, 433, 584, 599
AjiI CACGTC 1 cut(s) 53
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 4 cut(s) 14, 110, 278, 472
AluI AGCT 4 cut(s) 14, 110, 278, 472
AlwI GGATC 1 cut(s) 548
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 332
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 14, 144, 339
BceAI ACGGC 1 cut(s) 504
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 1 cut(s) 225
BfaI CTAG 2 cut(s) 198, 473
BfmI CTRYAG 2 cut(s) 15, 247
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 53
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 397
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 267
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 3 cut(s) 155, 331, 595
BseLI CCNNNNNNNGG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 119
BsgI GTGCAG 2 cut(s) 479, 507
Bsh1236I CGCG 1 cut(s) 418
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 521
Bsp143I GATC 2 cut(s) 553, 607
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 1 cut(s) 162
BspFNI CGCG 1 cut(s) 418
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 160
BspMAI CTGCAG 1 cut(s) 251
BspPI GGATC 1 cut(s) 548
BsrBI CCGCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 2 cut(s) 553, 607
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 2 cut(s) 43, 129
BstACI GRCGYC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 238
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 3 cut(s) 155, 331, 595
BstFNI CGCG 1 cut(s) 418
BstKTI GATC 2 cut(s) 556, 610
BstMBI GATC 2 cut(s) 553, 607
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 2 cut(s) 15, 247
BstUI CGCG 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BtgI CCRYGG 1 cut(s) 79
BtrI CACGTC 1 cut(s) 53
BtsCI GGATG 3 cut(s) 155, 331, 595
CciI TCATGA 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 267
CseI GACGC 1 cut(s) 273
CsiI ACCWGGT 1 cut(s) 223
Csp6I GTAC 1 cut(s) 291
CviAII CATG 3 cut(s) 80, 167, 368
CviJI RGCY 5 cut(s) 14, 110, 278, 472, 482
CviKI_1 RGCY 5 cut(s) 14, 110, 278, 472, 482
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 1 cut(s) 238
DpnI GATC 2 cut(s) 555, 609
DpnII GATC 2 cut(s) 553, 607
Eco130I CCWWGG 1 cut(s) 79
Eco32I GATATC 3 cut(s) 68, 90, 299
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 3 cut(s) 68, 90, 299
EcoT14I CCWWGG 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 3 cut(s) 83, 170, 371
FaqI GGGAC 1 cut(s) 257
FatI CATG 3 cut(s) 79, 166, 367
FauNDI CATATG 2 cut(s) 136, 657
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 3 cut(s) 162, 318, 602
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 2 cut(s) 198, 473
GluI GCNGC 1 cut(s) 108
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 273
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 3 cut(s) 83, 170, 371
HinfI GANTC 2 cut(s) 170, 210
HpaII CCGG 1 cut(s) 268
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 2 cut(s) 132, 663
Hpy188I TCNGA 3 cut(s) 11, 526, 558
Hpy188III TCNNGA 4 cut(s) 167, 198, 539, 569
Hpy8I GTNNAC 2 cut(s) 132, 663
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 317, 716
HpyCH4III ACNGT 2 cut(s) 43, 129
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 4 cut(s) 249, 344, 488, 496
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 1 cut(s) 52
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 3 cut(s) 83, 170, 371
Kzo9I GATC 2 cut(s) 553, 607
LmnI GCTCC 2 cut(s) 11, 283
LpnPI CCDG 8 cut(s) 48, 210, 237, 281, 348, 474, 497, 558
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 178
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 2 cut(s) 198, 473
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 555, 609
MbiI CCGCTC 1 cut(s) 164
MboI GATC 2 cut(s) 553, 607
MboII GAAGA 8 cut(s) 97, 353, 421, 497, 560, 563, 584, 719
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 553
MluCI AATT 6 cut(s) 141, 345, 387, 499, 530, 577
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 5 cut(s) 49, 148, 281, 372, 546
MseI TTAA 1 cut(s) 411
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 225
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 418
NcoI CCATGG 1 cut(s) 79
NdeI CATATG 2 cut(s) 136, 657
NdeII GATC 2 cut(s) 553, 607
NlaIII CATG 3 cut(s) 83, 170, 371
NlaIV GGNNCC 1 cut(s) 160
PagI TCATGA 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 160
PstI CTGCAG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 553, 607
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 225
SetI ASST 8 cut(s) 16, 55, 60, 112, 229, 280, 292, 474
SexAI ACCWGGT 1 cut(s) 223
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 2 cut(s) 15, 247
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 6 cut(s) 141, 345, 387, 499, 530, 577
SsiI CCGC 1 cut(s) 162
SspI AATATT 2 cut(s) 233, 408
SspMI CTAG 2 cut(s) 198, 473
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 2 cut(s) 43, 129
TaiI ACGT 1 cut(s) 55
TaqI TCGA 5 cut(s) 70, 262, 286, 295, 508
TasI AATT 6 cut(s) 141, 345, 387, 499, 530, 577
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 6 cut(s) 441, 543, 645, 705, 720, 735
XapI RAATTY 1 cut(s) 530
XbaI TCTAGA 1 cut(s) 197
XspI CTAG 2 cut(s) 198, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.