Rmu_sc0004511.1_g000001

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004511.1
OG0000697
Physical Location & Seq
Reverse (-)
633 .. 2110
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004511.1_g000001.1.cds

Sequence Viewer

Length: 786 bp
atgtctgaaatggacatgtatggagctgatgaagaggaagagcagttttatgaggctgttaagatattattaatggcaatacaagcattgatttatgtgttatacgaacttgtgttcagcatacatggtgaacatattagacgtccgctgacttgtcgtgaagctcagctgatatttgagcgatctcattttgctgttagcagaagtttcaacaaagtcttgaaggccttgaatacaatagcaccacagtttatggctaaacatgagtccataccacctaacataagagaaagggactgcggattcccaaatcgacgtcggttcctagctccatttcgaggtactcgatatcacctcaaagattttggtggtgaaggaaatcatcctgtcaatgcaattgagttatttaatcttagccatgcttccttgaggaacataattgagagaatatttagaatatttaagtcgcgtttcacaatcttcaaatcagcaccaccatttttatataagacacaagcagaactagttttggcttgtgcaggactgcacaattttcttcgacaggaatgtcgttcagatgaatttcctcctgaaccagaagaagatccgatagacaatcacgaagataattttgaatgggataattttcaaacccaagatcagcaaagagagaatgctaatgaatggataatgagtattgctactcatatgtggatagatgcccaaccaaatgccaacaatgaaaacaatgacaatcaagaaagtgaaaatgagggagaagaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

261

Amino Acids

30.64

Weight (kDa)

5.09

Isoelectric Point (pI)

62.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 567
AatII GACGTC 2 cut(s) 145, 319
AccII CGCG 1 cut(s) 469
AciI CCGC 2 cut(s) 146, 300
AclWI GGATC 1 cut(s) 599
AcsI RAATTY 1 cut(s) 581
AcyI GRCGYC 2 cut(s) 142, 316
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 6 cut(s) 211, 223, 232, 484, 635, 650
AhlI ACTAGT 1 cut(s) 523
AluBI AGCT 4 cut(s) 26, 164, 169, 329
AluI AGCT 4 cut(s) 26, 164, 169, 329
AlwI GGATC 1 cut(s) 599
AoxI GGCC 1 cut(s) 225
ApoI RAATTY 1 cut(s) 581
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 140, 344, 383
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 2 cut(s) 326, 524
BlpI GCTNAGC 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 323
BmsI GCATC 1 cut(s) 709
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 448
BsaHI GRCGYC 2 cut(s) 142, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 179
BsgI GTGCAG 2 cut(s) 530, 558
Bsh1236I CGCG 1 cut(s) 469
BshFI GGCC 1 cut(s) 227
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 1 cut(s) 227
Bsp143I GATC 3 cut(s) 182, 604, 658
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 2 cut(s) 146, 300
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 178
BspFNI CGCG 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 323
BspPI GGATC 1 cut(s) 599
BspQI GCTCTTC 1 cut(s) 33
BssMI GATC 3 cut(s) 182, 604, 658
BssNI GRCGYC 2 cut(s) 142, 316
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 2 cut(s) 27, 33
BstACI GRCGYC 2 cut(s) 142, 316
BstDEI CTNAG 2 cut(s) 165, 413
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 469
BstKTI GATC 3 cut(s) 185, 607, 661
BstMBI GATC 3 cut(s) 182, 604, 658
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstUI CGCG 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 1 cut(s) 227
BtsCI GGATG 1 cut(s) 382
Csp6I GTAC 1 cut(s) 342
CviAII CATG 4 cut(s) 16, 125, 263, 419
CviJI RGCY 9 cut(s) 26, 56, 164, 169, 227, 257, 329, 417, 533
CviKI_1 RGCY 9 cut(s) 26, 56, 164, 169, 227, 257, 329, 417, 533
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 2 cut(s) 165, 413
DpnI GATC 3 cut(s) 184, 606, 660
DpnII GATC 3 cut(s) 182, 604, 658
DrdI GACNNNNNNGTC 1 cut(s) 567
DseDI GACNNNNNNGTC 1 cut(s) 567
Eam1104I CTCTTC 2 cut(s) 27, 33
EarI CTCTTC 2 cut(s) 27, 33
Eco147I AGGCCT 1 cut(s) 227
Eco32I GATATC 1 cut(s) 350
EcoRV GATATC 1 cut(s) 350
FaeI CATG 4 cut(s) 19, 128, 266, 422
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 15, 124, 262, 418
FauNDI CATATG 1 cut(s) 708
FokI GGATG 1 cut(s) 369
FspBI CTAG 2 cut(s) 326, 524
HaeIII GGCC 1 cut(s) 227
Hin1I GRCGYC 2 cut(s) 142, 316
Hin1II CATG 4 cut(s) 19, 128, 266, 422
HinfI GANTC 2 cut(s) 266, 303
HphI GGTGA 3 cut(s) 140, 344, 383
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 3 cut(s) 7, 577, 609
Hpy188III TCNNGA 5 cut(s) 158, 220, 590, 620, 758
Hpy8I GTNNAC 1 cut(s) 131
Hpy99I CGWCG 2 cut(s) 318, 321
HpyAV CCTTC 2 cut(s) 217, 368
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 142, 316
HpyCH4V TGCA 3 cut(s) 395, 539, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 165, 413
HpySE526I ACGT 2 cut(s) 142, 316
Hsp92I GRCGYC 2 cut(s) 142, 316
Hsp92II CATG 4 cut(s) 19, 128, 266, 422
Kzo9I GATC 3 cut(s) 182, 604, 658
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 23, 334
LpnPI CCDG 5 cut(s) 399, 525, 548, 603, 609
LweI GCATC 1 cut(s) 709
MaeI CTAG 2 cut(s) 326, 524
MaeII ACGT 2 cut(s) 142, 316
MalI GATC 3 cut(s) 184, 606, 660
MboI GATC 3 cut(s) 182, 604, 658
MboII GAAGA 7 cut(s) 44, 50, 472, 548, 611, 614, 635
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 604
MluCI AATT 6 cut(s) 396, 438, 550, 581, 628, 643
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 7 cut(s) 28, 46, 332, 365, 423, 597, 766
MseI TTAA 4 cut(s) 60, 71, 408, 462
MspA1I CMGCKG 2 cut(s) 148, 169
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 679
MvnI CGCG 1 cut(s) 469
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeI CATATG 1 cut(s) 708
NdeII GATC 3 cut(s) 182, 604, 658
NlaIII CATG 4 cut(s) 19, 128, 266, 422
NlaIV GGNNCC 1 cut(s) 323
NspI RCATGY 1 cut(s) 19
PceI AGGCCT 1 cut(s) 227
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 1 cut(s) 303
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 323
PsuI RGATCY 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 4 cut(s) 60, 71, 408, 462
Sau3AI GATC 3 cut(s) 182, 604, 658
SchI GAGTC 1 cut(s) 275
SetI ASST 9 cut(s) 28, 145, 166, 171, 280, 319, 331, 343, 357
SfaNI GCATC 1 cut(s) 709
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 523
Sse9I AATT 6 cut(s) 396, 438, 550, 581, 628, 643
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 2 cut(s) 146, 300
SspI AATATT 2 cut(s) 450, 459
SspMI CTAG 2 cut(s) 326, 524
StuI AGGCCT 1 cut(s) 227
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 145, 319
TaqI TCGA 4 cut(s) 313, 337, 346, 559
TasI AATT 6 cut(s) 396, 438, 550, 581, 628, 643
TfiI GAWTC 1 cut(s) 303
Tru1I TTAA 4 cut(s) 60, 71, 408, 462
Tru9I TTAA 4 cut(s) 60, 71, 408, 462
TspDTI ATGAA 4 cut(s) 45, 594, 696, 756
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 581
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 326, 524
ZraI GACGTC 2 cut(s) 143, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.