FvH4_6g52063

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000697
Physical Location & Seq
Forward (+)
38470479 .. 38471957
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52063.t1

Sequence Viewer

Length: 597 bp
ATGTCTGAAATGGATATGTATGGAGTTGATGAAGAGGAAGAAGAGTTTTACGAGGCCATTAGAATTTTATTAATGGCAATACAAGCAGTGATTTATGTGTTATATGATCTTGTGCTCAGCATACCAGATGAAAGTATTAGACGTCCATTGACTCGGAGACCAGTGACATCAAATGGGTACATATACATGAATAAGATACTGGACGGAGACCCTCATATCTTTCGGGAGGTTTACAGAATGTATCCTGACGTGTTCCGGAAATTGTGTGTCATCATAAAAGAGAAAACACCTTTACGGAATACAAGACACATTTGTGTTGAAGAAATGCTTGCAACCTTTTTACTAGTTGTCGGATTGCATAATTTTCTTCGACAAGAATGCCGTTCAGATGAATTTCCTCCTGAACCAGAAGATGATCCGGTAGAGAATGAAGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGAGCAGCAAAGAGATAATGCTAATCAATGGAGAATGAGTGTTGCTAATCAGATGTGGGCAGAAGCTCAAGCAAATGCCAACAATGTAAACAATGACAATGAAGAAAGTAATCATGAAGGAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

199

Amino Acids

23.5

Weight (kDa)

4.38

Isoelectric Point (pI)

64.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 58 - 124 2.7e-14 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccIII TCCGGA 1 cut(s) 255
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 2 cut(s) 63, 392
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 3 cut(s) 320, 446, 461
AhlI ACTAGT 1 cut(s) 343
AjiI CACGTC 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 312
AluBI AGCT 1 cut(s) 533
AluI AGCT 1 cut(s) 533
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 151, 201
AlwI GGATC 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 255
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 2 cut(s) 63, 392
AseI ATTAAT 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 484
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciVI GTATCC 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 151, 201
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 1 cut(s) 344
BfuI GTATCC 1 cut(s) 252
BisI GCNGC 1 cut(s) 473
BlpI GCTNAGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 474
BmgBI CACGTC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 2 cut(s) 151, 201
BsaWI WCCGGW 2 cut(s) 255, 418
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bse1I ACTGG 2 cut(s) 161, 204
BseAI TCCGGA 1 cut(s) 255
BseGI GGATG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 130
BseNI ACTGG 2 cut(s) 161, 204
BseXI GCAGC 1 cut(s) 484
BshFI GGCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 256, 419
BsmAI GTCTC 2 cut(s) 151, 201
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 2 cut(s) 151, 201
Bsp1286I GDGCHC 1 cut(s) 117
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 2 cut(s) 106, 415
Bsp1720I GCTNAGC 1 cut(s) 116
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 129
BspEI TCCGGA 1 cut(s) 255
BspHI TCATGA 1 cut(s) 580
BspPI GGATC 1 cut(s) 410
BspTNI GGTCTC 2 cut(s) 151, 201
BsrI ACTGG 2 cut(s) 161, 204
BssMI GATC 2 cut(s) 106, 415
BssNI GRCGYC 1 cut(s) 142
Bst6I CTCTTC 2 cut(s) 27, 36
BstACI GRCGYC 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 116
BstF5I GGATG 1 cut(s) 457
BstKTI GATC 2 cut(s) 109, 418
BstMAI GTCTC 2 cut(s) 151, 201
BstMBI GATC 2 cut(s) 106, 415
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 484
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 1 cut(s) 56
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 168
Cac8I GCNNGC 1 cut(s) 330
CciI TCATGA 1 cut(s) 580
Csp6I GTAC 1 cut(s) 178
CviAII CATG 2 cut(s) 187, 581
CviJI RGCY 2 cut(s) 56, 533
CviKI_1 RGCY 2 cut(s) 56, 533
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 1 cut(s) 116
DpnI GATC 2 cut(s) 108, 417
DpnII GATC 2 cut(s) 106, 415
Eam1104I CTCTTC 2 cut(s) 27, 36
EarI CTCTTC 2 cut(s) 27, 36
Eco31I GGTCTC 2 cut(s) 151, 201
FaeI CATG 2 cut(s) 190, 584
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 2 cut(s) 186, 580
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 1 cut(s) 344
GluI GCNGC 1 cut(s) 473
HaeIII GGCC 1 cut(s) 56
HapII CCGG 2 cut(s) 256, 419
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 2 cut(s) 190, 584
HinfI GANTC 1 cut(s) 151
HpaII CCGG 2 cut(s) 256, 419
Hpy166II GTNNAC 2 cut(s) 232, 556
Hpy188I TCNGA 5 cut(s) 7, 156, 353, 388, 519
Hpy188III TCNNGA 5 cut(s) 224, 245, 256, 401, 581
Hpy8I GTNNAC 2 cut(s) 232, 556
HpyAV CCTTC 1 cut(s) 578
HpyCH4IV ACGT 2 cut(s) 142, 249
HpyCH4V TGCA 2 cut(s) 332, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 116
HpySE526I ACGT 2 cut(s) 142, 249
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 2 cut(s) 190, 584
Kpn2I TCCGGA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 106, 415
LpnPI CCDG 8 cut(s) 138, 174, 185, 258, 269, 414, 420, 432
Lsp1109I GCAGC 1 cut(s) 484
MaeI CTAG 1 cut(s) 344
MaeII ACGT 2 cut(s) 142, 249
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 108, 417
MboI GATC 2 cut(s) 106, 415
MboII GAAGA 9 cut(s) 44, 50, 53, 332, 359, 422, 443, 446, 581
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 5 cut(s) 63, 260, 361, 392, 439
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 5 cut(s) 28, 46, 220, 222, 408
MroI TCCGGA 1 cut(s) 255
MseI TTAA 1 cut(s) 71
MslI CAYNNNNRTG 2 cut(s) 185, 312
MspI CCGG 2 cut(s) 256, 419
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 2 cut(s) 106, 415
NlaIII CATG 2 cut(s) 190, 584
NmuCI GTSAC 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 312
PagI TCATGA 1 cut(s) 580
PctI GAATGC 1 cut(s) 383
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PshBI ATTAAT 1 cut(s) 71
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 2 cut(s) 185, 312
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 2 cut(s) 106, 415
SchI GAGTC 1 cut(s) 145
SduI GDGCHC 1 cut(s) 117
SetI ASST 6 cut(s) 145, 231, 252, 292, 338, 535
SmiMI CAYNNNNRTG 2 cut(s) 185, 312
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 343
Sse9I AATT 5 cut(s) 63, 260, 361, 392, 439
SspMI CTAG 1 cut(s) 344
TaiI ACGT 2 cut(s) 145, 252
TaqI TCGA 1 cut(s) 370
TasI AATT 5 cut(s) 63, 260, 361, 392, 439
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 2 cut(s) 93, 168
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 7 cut(s) 45, 144, 203, 405, 444, 582, 597
TspGWI ACGGA 2 cut(s) 219, 310
TspRI CASTG 2 cut(s) 93, 168
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 2 cut(s) 63, 392
XspI CTAG 1 cut(s) 344
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.