Rmu_sc0002357.1_g000042

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002357.1
OG0000697
Physical Location & Seq
Forward (+)
153437 .. 154879
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002357.1_g000042.1.cds

Sequence Viewer

Length: 834 bp
atggatgtcatgaatgaagatgatatggatgatgaagaattttttgaagctataaagggtatcttgatggcaatagaagcaattatccatctagattgtataggagcgattgatgggacacatattccagcaatggtgaagggacctgagataagcgcataccgaaaccgatatggaaaaacatcacaaaatgtattggctgctactaactttgacttggaattcatttatgtgctcagtgggtgggaaggttcagctcatgattcaagagtactaaatgatgcattgacaaggagaaacgggcttaagttacctccaggtaaatttttattggtggatgctggatttgcaaatagacatggtttcttagcacctattagaggcgtccgctatcatctcaaagagttcagtggtcagggtcgccaccctgaaaatgcgcgtgagttattcaatcttcgtcatgcttccttgagaaacgtagttgagagaatatttggagtacttaaatcccgtttcacaatattcaagactgcacctccattcccatttaagacacaagcggagccagtgttggcttgtgcaggacttcacaactttcttcgtaaggaatgtcgttctgatttatttcctgttgaaccggaggatgaagaagattatgaacatgaagaagatccgaaagatgaattccaaacccaagaccaacaaagagaggaagctaatgattggagaatggagatagctaatcatatgtggagagatgcaagacctgcacctgatcctgaacccaatcctccacccgttgttgaaggcaacaatgcagcagaacaatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

277

Amino Acids

31.51

Weight (kDa)

5.01

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 775
AccII CGCG 1 cut(s) 439
AciI CCGC 2 cut(s) 388, 560
AclWI GGATC 2 cut(s) 665, 770
AcsI RAATTY 4 cut(s) 38, 221, 323, 683
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 2 cut(s) 273, 501
AfiI CCNNNNNNNGG 1 cut(s) 380
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 6 cut(s) 47, 267, 451, 526, 635, 806
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 4 cut(s) 50, 257, 716, 740
AluI AGCT 4 cut(s) 50, 257, 716, 740
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 2 cut(s) 665, 770
ApeKI GCWGC 2 cut(s) 200, 818
ApoI RAATTY 4 cut(s) 38, 221, 323, 683
AspLEI GCGC 2 cut(s) 158, 439
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 187, 830
BccI CCATC 3 cut(s) 61, 96, 107
BciT130I CCWGG 1 cut(s) 318
BfaI CTAG 1 cut(s) 92
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 775
BisI GCNGC 2 cut(s) 201, 819
BlsI GCNGC 2 cut(s) 202, 820
BmcAI AGTACT 2 cut(s) 273, 501
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 144, 564
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 3 cut(s) 271, 328, 748
BpmI CTGGAG 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 490
BsaHI GRCGYC 1 cut(s) 384
BsaWI WCCGGW 1 cut(s) 637
BsaXI ACNNNNNCTCC 4 cut(s) 520, 550, 554, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 138
BseBI CCWGG 1 cut(s) 318
BseGI GGATG 4 cut(s) 10, 34, 343, 649
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 138, 250
BseNI ACTGG 1 cut(s) 566
BseXI GCAGC 2 cut(s) 187, 830
BsgI GTGCAG 3 cut(s) 516, 600, 753
Bsh1236I CGCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 638
BslFI GGGAC 2 cut(s) 130, 156
BslI CCNNNNNNNGG 1 cut(s) 380
BsmFI GGGAC 2 cut(s) 130, 156
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 670, 775
BspACI CCGC 2 cut(s) 388, 560
BspCNI CTCAG 2 cut(s) 139, 249
BspFNI CGCG 1 cut(s) 439
BspHI TCATGA 3 cut(s) 9, 259, 830
BspLI GGNNCC 2 cut(s) 144, 564
BspMI ACCTGC 1 cut(s) 775
BspPI GGATC 2 cut(s) 665, 770
BspTI CTTAAG 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 1 cut(s) 566
BssMI GATC 2 cut(s) 670, 775
BssNI GRCGYC 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 384
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 767
BstDEI CTNAG 3 cut(s) 147, 236, 367
BstENI CCTNNNNNAGG 1 cut(s) 378
BstF5I GGATG 4 cut(s) 10, 34, 343, 649
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 2 cut(s) 158, 439
BstKTI GATC 2 cut(s) 673, 778
BstMBI GATC 2 cut(s) 670, 775
BstMWI GCNNNNNNNGC 3 cut(s) 77, 347, 767
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 187, 830
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BtsCI GGATG 4 cut(s) 10, 34, 343, 649
BtsIMutI CAGTG 3 cut(s) 244, 415, 573
BveI ACCTGC 1 cut(s) 775
CciI TCATGA 3 cut(s) 9, 259, 830
CfoI GCGC 2 cut(s) 158, 439
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 373
Csp6I GTAC 2 cut(s) 272, 500
CviAII CATG 6 cut(s) 10, 260, 359, 461, 662, 831
CviJI RGCY 8 cut(s) 50, 200, 257, 304, 565, 575, 716, 740
CviKI_1 RGCY 8 cut(s) 50, 200, 257, 304, 565, 575, 716, 740
CviQI GTAC 2 cut(s) 272, 500
DdeI CTNAG 3 cut(s) 147, 236, 367
DpnI GATC 2 cut(s) 672, 777
DpnII GATC 2 cut(s) 670, 775
Eco47I GGWCC 1 cut(s) 143
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 143
EcoRI GAATTC 2 cut(s) 221, 683
EcoRII CCWGG 1 cut(s) 316
EcoT22I ATGCAT 1 cut(s) 286
FaeI CATG 6 cut(s) 13, 263, 362, 464, 665, 834
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 2 cut(s) 130, 156
FatI CATG 6 cut(s) 9, 259, 358, 460, 661, 830
FauNDI CATATG 1 cut(s) 747
Fnu4HI GCNGC 2 cut(s) 201, 819
FokI GGATG 4 cut(s) 17, 41, 350, 656
Fsp4HI GCNGC 2 cut(s) 201, 819
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 157, 438
GluI GCNGC 2 cut(s) 201, 819
GsuI CTGGAG 1 cut(s) 300
HapII CCGG 1 cut(s) 638
HgaI GACGC 1 cut(s) 373
HhaI GCGC 2 cut(s) 158, 439
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 6 cut(s) 13, 263, 362, 464, 665, 834
Hin6I GCGC 2 cut(s) 156, 437
HinP1I GCGC 2 cut(s) 156, 437
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 638
HphI GGTGA 1 cut(s) 148
Hpy188I TCNGA 2 cut(s) 619, 675
Hpy188III TCNNGA 8 cut(s) 10, 64, 92, 260, 267, 526, 779, 831
HpyAV CCTTC 3 cut(s) 133, 242, 800
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 7 cut(s) 284, 350, 533, 581, 761, 770, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 347, 767
HpyF3I CTNAG 3 cut(s) 147, 236, 367
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 6 cut(s) 13, 263, 362, 464, 665, 834
HspAI GCGC 2 cut(s) 156, 437
Kzo9I GATC 2 cut(s) 670, 775
LmnI GCTCC 2 cut(s) 104, 562
Lsp1109I GCAGC 2 cut(s) 187, 830
LweI GCATC 3 cut(s) 271, 328, 748
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 309
MalI GATC 2 cut(s) 672, 777
MboI GATC 2 cut(s) 670, 775
MboII GAAGA 8 cut(s) 29, 47, 446, 590, 659, 662, 677, 680
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 5 cut(s) 38, 81, 221, 323, 683
MnlI CCTC 6 cut(s) 324, 374, 546, 634, 703, 801
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 3 cut(s) 306, 504, 549
MslI CAYNNNNRTG 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 1 cut(s) 638
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 3 cut(s) 77, 347, 767
NdeI CATATG 1 cut(s) 747
NdeII GATC 2 cut(s) 670, 775
NlaIII CATG 6 cut(s) 13, 263, 362, 464, 665, 834
NlaIV GGNNCC 2 cut(s) 144, 564
NsiI ATGCAT 1 cut(s) 286
PagI TCATGA 3 cut(s) 9, 259, 830
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 202, 820
PpuMI RGGWCCY 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 144, 564
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
PsuI RGATCY 1 cut(s) 670
RsaI GTAC 2 cut(s) 273, 501
RsaNI GTAC 2 cut(s) 272, 500
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 306, 504, 549
SatI GCNGC 2 cut(s) 201, 819
Sau3AI GATC 2 cut(s) 670, 775
Sau96I GGNCC 1 cut(s) 143
ScaI AGTACT 2 cut(s) 273, 501
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 3 cut(s) 271, 328, 748
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 2 cut(s) 305, 469
SmoI CTYRAG 2 cut(s) 305, 469
Sse9I AATT 5 cut(s) 38, 81, 221, 323, 683
SsiI CCGC 2 cut(s) 388, 560
SspI AATATT 2 cut(s) 492, 522
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 316
TaiI ACGT 1 cut(s) 480
TasI AATT 5 cut(s) 38, 81, 221, 323, 683
TatI WGTACW 2 cut(s) 271, 499
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 306, 504, 549
Tru9I TTAA 3 cut(s) 306, 504, 549
TscAI CASTG 3 cut(s) 244, 415, 573
TseI GCWGC 2 cut(s) 200, 818
TspDTI ATGAA 8 cut(s) 26, 30, 48, 214, 660, 672, 678, 696
TspRI CASTG 3 cut(s) 244, 415, 573
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 378
XapI RAATTY 4 cut(s) 38, 221, 323, 683
XbaI TCTAGA 1 cut(s) 91
XspI CTAG 1 cut(s) 92
ZrmI AGTACT 2 cut(s) 273, 501
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.