FvH4_4g17331

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000697
Physical Location & Seq
Forward (+)
21272334 .. 21274309
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17331.t1

Sequence Viewer

Length: 909 bp
ATGGACGAGGATGAAATTAATGGACAAGAGATAGGGGGAGAAGAAATAGATGAAGAAGAAATTGAAGAAATAGATGAAGAAGAAATTGAAGAGGAATTTTATGAAACAATCAAGTCTAATTGTATCGGTGCAATTGATGGCACCCATATTCCAGCAATGGTAAAAGGTCGTGAAGTAAGTAGCTACTGTAATCGTCATGGAATTCAATCTCAAAATGTCTTGGCAGCTTGCAACCTTGATTTACAATTCATATATGTGCTAAGTGGATGGGAAGGTTCGGCATATGATTCAAAATTGTTAAATGATGCCCTATCAAGAAGGAATGGACTTGAAGTTCCTCAAGGCAAATATTTTCTTGTGGATTGTGGATTTGCTAATCGACGTCAATTCTTAGCTCCATTACGAGGTGTTAGATATCATCTTAAGGATTTTGGTGGTCAAGGTCGCCATCCCCGAAATGCAAGTGAGTTGTTCAATCTTCGTCATGCATCATTGAGGAATGTGATTGAGAGGATATTTGGTATCTTTAAGTCACAGTTCACAATTTTCAAAACCGCACCTCCATTCCCATATAAAACACAAGCAGAATTGGTGTTAGCTTGTGCTGGACTACACAACTTTCTTCGCAAAGAATGTTGTTCCGATGAATTTCCTGTTGAACCAGAAAATGATTCATCATCTTCATCCTATCTAGATATGGAAGATGAGAATCTTGAACTTCTTTCTCAAAGTCAACTACGGCAAAGAGCAGAGGCTAATGCTTGGAGGCTTTCTATTGCTGAAGCTATGTGGCGAGATAGACCGCGAAATGGTGATAATGGAAGTCAAGAGGACAACAATGAGAATGAGAATATTGAGGAACATATGGATGATGTGGATGATGATGAAGAAGATTATGACGATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

303

Amino Acids

34.56

Weight (kDa)

4.41

Isoelectric Point (pI)

68.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_1 PF01609 40 - 185 7.3e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 45 - 206 1.2e-22 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 140
AccII CGCG 1 cut(s) 805
AciI CCGC 2 cut(s) 555, 803
AcsI RAATTY 3 cut(s) 95, 201, 647
AcuI CTGAAG 1 cut(s) 801
AcyI GRCGYC 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 404, 809
AflII CTTAAG 1 cut(s) 422
AgsI TTSAA 9 cut(s) 65, 89, 206, 291, 332, 475, 550, 659, 716
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 5 cut(s) 183, 227, 395, 599, 785
AluI AGCT 5 cut(s) 183, 227, 395, 599, 785
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 3 cut(s) 95, 201, 647
AseI ATTAAT 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 824
BanI GGYRCC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 236
BccI CCATC 3 cut(s) 131, 261, 456
BceAI ACGGC 1 cut(s) 755
BfaI CTAG 1 cut(s) 692
BfrI CTTAAG 1 cut(s) 422
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 142
BmsI GCATC 2 cut(s) 295, 497
BpuEI CTTGAG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 809
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 708
BseGI GGATG 6 cut(s) 16, 272, 448, 683, 874, 883
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 2 cut(s) 404, 809
BseMI GCAATG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 805
BshNI GGYRCC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 404, 809
BspACI CCGC 2 cut(s) 555, 803
BspFNI CGCG 1 cut(s) 805
BspLI GGNNCC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 140
BspTI CTTAAG 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 162
BssNI GRCGYC 1 cut(s) 382
Bst4CI ACNGT 2 cut(s) 188, 537
Bst6I CTCTTC 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 382
BstAFI CTTAAG 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 260, 391
BstF5I GGATG 6 cut(s) 16, 272, 448, 683, 874, 883
BstFNI CGCG 1 cut(s) 805
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 1 cut(s) 236
BtsCI GGATG 6 cut(s) 16, 272, 448, 683, 874, 883
Cac8I GCNNGC 1 cut(s) 229
CviAII CATG 2 cut(s) 197, 485
CviJI RGCY 7 cut(s) 183, 227, 395, 599, 755, 769, 785
CviKI_1 RGCY 7 cut(s) 183, 227, 395, 599, 755, 769, 785
DdeI CTNAG 2 cut(s) 260, 391
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco32I GATATC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 801
EcoRI GAATTC 1 cut(s) 201
EcoRV GATATC 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 490
FaeI CATG 2 cut(s) 200, 488
FatI CATG 2 cut(s) 196, 484
FauNDI CATATG 2 cut(s) 283, 864
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 6 cut(s) 23, 279, 435, 670, 881, 890
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 692
GluI GCNGC 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 2 cut(s) 200, 488
HincII GTYRAC 1 cut(s) 734
HindII GTYRAC 1 cut(s) 734
HinfI GANTC 3 cut(s) 287, 671, 709
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 2 cut(s) 540, 734
Hpy188I TCNGA 1 cut(s) 643
Hpy188III TCNNGA 5 cut(s) 170, 315, 692, 713, 827
Hpy8I GTNNAC 2 cut(s) 540, 734
Hpy99I CGWCG 1 cut(s) 384
HpyAV CCTTC 2 cut(s) 266, 312
HpyCH4III ACNGT 2 cut(s) 188, 537
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 4 cut(s) 131, 231, 461, 488
HpyF3I CTNAG 2 cut(s) 260, 391
HpySE526I ACGT 1 cut(s) 382
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 2 cut(s) 200, 488
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 4 cut(s) 165, 591, 666, 675
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 2 cut(s) 295, 497
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 531
MfeI CAATTG 1 cut(s) 132
Mph1103I ATGCAT 1 cut(s) 490
MseI TTAA 4 cut(s) 18, 299, 423, 528
MslI CAYNNNNRTG 2 cut(s) 254, 867
MspCI CTTAAG 1 cut(s) 422
MunI CAATTG 1 cut(s) 132
MvnI CGCG 1 cut(s) 805
NdeI CATATG 2 cut(s) 283, 864
NlaIII CATG 2 cut(s) 200, 488
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 531
NsiI ATGCAT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 451
PfeI GAWTC 3 cut(s) 287, 671, 709
PkrI GCNGC 1 cut(s) 226
PshBI ATTAAT 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 142
RseI CAYNNNNRTG 2 cut(s) 254, 867
SaqAI TTAA 4 cut(s) 18, 299, 423, 528
SatI GCNGC 1 cut(s) 225
SfaNI GCATC 2 cut(s) 295, 497
SmiMI CAYNNNNRTG 2 cut(s) 254, 867
SmlI CTYRAG 2 cut(s) 339, 422
SmoI CTYRAG 2 cut(s) 339, 422
SsiI CCGC 2 cut(s) 555, 803
SspI AATATT 2 cut(s) 350, 853
SspMI CTAG 1 cut(s) 692
TaaI ACNGT 2 cut(s) 188, 537
TaiI ACGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 379
TfiI GAWTC 3 cut(s) 287, 671, 709
Tru1I TTAA 4 cut(s) 18, 299, 423, 528
Tru9I TTAA 4 cut(s) 18, 299, 423, 528
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 9 cut(s) 27, 66, 90, 117, 238, 660, 663, 672, 900
Vha464I CTTAAG 1 cut(s) 422
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 3 cut(s) 95, 201, 647
XbaI TCTAGA 1 cut(s) 691
XspI CTAG 1 cut(s) 692
ZraI GACGTC 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.