Rw7G036830

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000697
Physical Location & Seq
Reverse (-)
55795001 .. 55796462
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036830.1

Sequence Viewer

Length: 846 bp
ATGTCTGAAATGGACATGTATGGAGCTGATGAAGAGGAAGAACAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAATACAAGCAGTGGTTTATGTGTTATACGACCTTGTATTCAGCATACGTGGTGAACGTATTAGACGTCCACTGACTCGGCGACCAGTGACATCAAGTGGATACATATATATGCACAAAATATTAGACAGAGACCCTCAAATCTTTAGAGAGGTGTATAGAATGTATCCTGCTGAAGCTCGGCTGATATTTGAGCGATCTCATTTCACTGTTAGCAAAAGTTTCAATAAAGTCTTGAAGGCCTTGAATACAATAGCACCTGAGTTTATGGCTAAACCTGAGTCCGTGCCACCCAACATAAGAGAAAGTACAAGGTTTTATCCTTACTTTAAGGATTGCGTCGGAGCTATAAATGGCACACATATTCCAGCAACGGTAGTTGGACGTGAGGTTAGCAGATATCGAAATCGACATGGGAAGATATCACAAAATGTATTAGCAGCTTGTAACTTTGATTTACAGTTCACATATGTAATTAGTGGATGGGAGGGTTCCGCTCATGATTCAAAAGTATTGAATGATACGATTTCTAGACGAAATGGACTCAAAGAATGCCGTTCAGATGAATTTCCTCCCGAACCAGAAGAAGATCCGATAGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCAGCAGAGAGAAAATGCTAATGAATGGAGAATGAGTATTGCTACTCATATGTGGACAGATGCCCAACCAAATGCCAACAATGACAACAATAACAATGAAGAAAGTGAAAATGAAGGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

281

Amino Acids

32.8

Weight (kDa)

4.95

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 143 - 201 2.8e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 572
AciI CCGC 1 cut(s) 570
AclWI GGATC 1 cut(s) 659
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 7 cut(s) 301, 313, 322, 582, 592, 695, 710
AjiI CACGTC 1 cut(s) 461
AluBI AGCT 4 cut(s) 26, 254, 422, 518
AluI AGCT 4 cut(s) 26, 254, 422, 518
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 659
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 1 cut(s) 641
AseI ATTAAT 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 140
BbvI GCAGC 1 cut(s) 527
BccI CCATC 1 cut(s) 552
BceAI ACGGC 1 cut(s) 615
BciVI GTATCC 2 cut(s) 170, 252
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 606
BfuI GTATCC 2 cut(s) 170, 252
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
BmgBI CACGTC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 568
BmsI GCATC 1 cut(s) 769
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bse1I ACTGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 563, 706
BseMII CTCAG 2 cut(s) 327, 345
BseNI ACTGG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 1 cut(s) 317
Bso31I GGTCTC 1 cut(s) 201
Bsp143I GATC 3 cut(s) 272, 664, 718
BspACI CCGC 1 cut(s) 570
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 328, 346
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 572
BsrI ACTGG 1 cut(s) 161
BssMI GATC 3 cut(s) 272, 664, 718
BssNI GRCGYC 1 cut(s) 142
Bst4CI ACNGT 4 cut(s) 45, 286, 451, 537
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 142
BstBAI YACGTR 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 336, 354
BstF5I GGATG 2 cut(s) 563, 706
BstKTI GATC 3 cut(s) 275, 667, 721
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 3 cut(s) 272, 664, 718
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuI GTATCC 2 cut(s) 170, 252
BsuRI GGCC 1 cut(s) 317
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 563, 706
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 4 cut(s) 93, 146, 168, 282
CciI TCATGA 1 cut(s) 574
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 384
CviAII CATG 3 cut(s) 16, 488, 575
CviJI RGCY 8 cut(s) 26, 56, 254, 259, 317, 347, 422, 518
CviKI_1 RGCY 8 cut(s) 26, 56, 254, 259, 317, 347, 422, 518
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 336, 354
DpnI GATC 3 cut(s) 274, 666, 720
DpnII GATC 3 cut(s) 272, 664, 718
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 2 cut(s) 476, 498
Eco57I CTGAAG 1 cut(s) 270
EcoRV GATATC 2 cut(s) 476, 498
FaeI CATG 3 cut(s) 19, 491, 578
FatI CATG 3 cut(s) 15, 487, 574
FauNDI CATATG 2 cut(s) 544, 768
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 2 cut(s) 570, 713
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 1 cut(s) 606
GluI GCNGC 1 cut(s) 516
HaeIII GGCC 1 cut(s) 317
HgaI GACGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 3 cut(s) 19, 491, 578
HinfI GANTC 4 cut(s) 151, 356, 578, 618
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 4 cut(s) 131, 146, 540, 774
Hpy188I TCNGA 4 cut(s) 7, 419, 637, 669
Hpy188III TCNNGA 5 cut(s) 310, 575, 606, 650, 680
Hpy8I GTNNAC 4 cut(s) 131, 146, 540, 774
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 2 cut(s) 307, 827
HpyCH4III ACNGT 4 cut(s) 45, 286, 451, 537
HpyCH4IV ACGT 4 cut(s) 124, 133, 142, 460
HpyCH4V TGCA 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 336, 354
HpySE526I ACGT 4 cut(s) 124, 133, 142, 460
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 3 cut(s) 19, 491, 578
Kzo9I GATC 3 cut(s) 272, 664, 718
LmnI GCTCC 2 cut(s) 23, 419
LpnPI CCDG 6 cut(s) 174, 258, 348, 366, 456, 669
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 606
MaeII ACGT 4 cut(s) 124, 133, 142, 460
MaeIII GTNAC 2 cut(s) 163, 521
MalI GATC 3 cut(s) 274, 666, 720
MbiI CCGCTC 1 cut(s) 572
MboI GATC 3 cut(s) 272, 664, 718
MboII GAAGA 7 cut(s) 44, 50, 505, 671, 674, 695, 830
MflI RGATCY 1 cut(s) 664
MluCI AATT 3 cut(s) 549, 641, 688
MlyI GAGTC 3 cut(s) 145, 365, 612
MmeI TCCRAC 2 cut(s) 397, 436
MnlI CCTC 7 cut(s) 28, 46, 220, 222, 457, 556, 657
MseI TTAA 3 cut(s) 60, 71, 405
MslI CAYNNNNRTG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 632
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeI CATATG 2 cut(s) 544, 768
NdeII GATC 3 cut(s) 272, 664, 718
NlaIII CATG 3 cut(s) 19, 491, 578
NlaIV GGNNCC 1 cut(s) 568
NmeAIII GCCGAG 2 cut(s) 133, 235
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 19
PagI TCATGA 1 cut(s) 574
PceI AGGCCT 1 cut(s) 317
PciI ACATGT 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 139
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 578
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 3 cut(s) 145, 364, 612
PpsI GAGTC 3 cut(s) 145, 364, 612
Ppu21I YACGTR 1 cut(s) 125
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 568
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 3 cut(s) 60, 71, 405
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 3 cut(s) 272, 664, 718
SchI GAGTC 3 cut(s) 145, 365, 612
SfaNI GCATC 1 cut(s) 769
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 3 cut(s) 549, 641, 688
SseBI AGGCCT 1 cut(s) 317
SsiI CCGC 1 cut(s) 570
SspI AATATT 1 cut(s) 198
SspMI CTAG 1 cut(s) 606
StuI AGGCCT 1 cut(s) 317
TaaI ACNGT 4 cut(s) 45, 286, 451, 537
TaiI ACGT 4 cut(s) 127, 136, 145, 463
TaqI TCGA 2 cut(s) 478, 484
TasI AATT 3 cut(s) 549, 641, 688
TatI WGTACW 1 cut(s) 383
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 3 cut(s) 60, 71, 405
Tru9I TTAA 3 cut(s) 60, 71, 405
TscAI CASTG 4 cut(s) 93, 153, 168, 289
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 45, 654, 756, 831, 846
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 4 cut(s) 93, 153, 168, 289
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 605
XceI RCATGY 1 cut(s) 19
XspI CTAG 1 cut(s) 606
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.