Rw6G003640
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000697
Physical Location & Seq
Forward (+)
5969979 .. 5971370
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003640.1

Sequence Viewer

Length: 1047 bp
ATGTATCCGGATGTCTTTCTAAAGTTGTGCACTATTATTAGAGAGAGAACTGCTTTGGAAGATACAAGATATATTTGTGTCGAAGAAAGGGTAGCTATATTCTTAATGATTGTGGGGCATAATGAACGATATTGTAATGTTCGTCAAAGGTTTGGTCGCTCACATTTCGTAACTAGTCAAAACTTCAACAAAGTTTTAAAGGCCTTGAACACTATGGCACCACAAATGATGGTCAAACCTGGAGGTGTGCCCTCTAAAATTAGGGAAAGTACAAGGTTTTACCCTTATTTTAAGGATTGCATTGGTGCTATTGATGGAACCCACATTCCAACCATGGTAAAAGGTCGAGAAGTAAGCAGCTATCGTAACCGACATGGTATTCAATCTCAAAATGTTTTGGCTGCTTGCAATTTCGATTTGGAATTCAAATACATGCTTAGTGGGTGGGAAGGTTCGGCACATGATTCAAAGTTGCTAAATGATGCCTTATCAAGAAGAAATGGAATTGAAGTGCCTCAAGGAAAATATTTTCTCGTGGATTGTGGATTTGCTAATCAACATTTTGGTGGTCAAGGTCGCCACCCCAAAAATGCAAGTGAGAATGTGATTGAAATGATTTTTGGTATCTTTAAATCACGGTTCACAATTTTCAAAATCGCACTTCCCTTCCCATTTGAGACACAAGCGGAGTTAGTGTTAGCTTGTGCTAGACTACATAACTTTCTTCGCAAAGAATGTCGCTCCGATGAATTTCCTGTTGAACCAGAAGATGATCAGTTTTCATCATATCTAGGCATGGAAGATGAAAATCCTGAACTACTTTCTCAAAGCCAACAACAACAAAGGGCGGAAGCTAATGATTGGAGAATTAGCATTGCTGATGCTATGTGGAATGATAGGCCGCGGAATGATGATAATGGAAATCAAGAGGATAACAATGAGGATCAAGACAATGACAATGAGAATAATGAGGAACACATAAATGATGAGAATCAAGAGGTTTACGATGATAATGAGGTTGGAATGGAGGAGTATGCATCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

348

Amino Acids

40.25

Weight (kDa)

4.98

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 69 7e-19 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 104 - 240 4.1e-13 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccII CGCG 1 cut(s) 904
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 4 cut(s) 686, 848, 902, 904
AclWI GGATC 1 cut(s) 951
AcsI RAATTY 2 cut(s) 422, 749
AfaI GTAC 1 cut(s) 271
AgsI TTSAA 9 cut(s) 187, 208, 383, 427, 468, 509, 611, 652, 761
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AloI GAACNNNNNNTCC 2 cut(s) 310, 342
AluBI AGCT 4 cut(s) 95, 360, 701, 854
AluI AGCT 4 cut(s) 95, 360, 701, 854
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 671
Alw44I GTGCAC 1 cut(s) 28
AlwI GGATC 1 cut(s) 951
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 201, 899
ApaLI GTGCAC 1 cut(s) 28
ApeKI GCWGC 2 cut(s) 357, 401
ApoI RAATTY 2 cut(s) 422, 749
ArsI GACNNNNNNTTYG 2 cut(s) 139, 171
BaeGI GKGCMC 2 cut(s) 32, 252
BanI GGYRCC 1 cut(s) 217
BauI CACGAG 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 369, 388
BccI CCATC 2 cut(s) 223, 308
BciT130I CCWGG 1 cut(s) 240
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 1 cut(s) 671
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 3 cut(s) 174, 708, 791
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 3 cut(s) 358, 402, 902
BlsI GCNGC 3 cut(s) 359, 403, 903
Bme1390I CCNGG 1 cut(s) 240
BmiI GGNNCC 2 cut(s) 219, 319
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 2 cut(s) 472, 871
BpmI CTGGAG 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 501
BsaBI GATNNNNATC 2 cut(s) 807, 990
BsaJI CCNNGG 2 cut(s) 333, 902
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bse3DI GCAATG 1 cut(s) 873
Bse8I GATNNNNATC 2 cut(s) 807, 990
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 333, 902
BseGI GGATG 1 cut(s) 16
BseJI GATNNNNATC 2 cut(s) 807, 990
BseMI GCAATG 1 cut(s) 873
BseRI GAGGAG 1 cut(s) 1043
BseSI GKGCMC 2 cut(s) 32, 252
BseXI GCAGC 2 cut(s) 369, 388
Bsh1236I CGCG 1 cut(s) 904
BshFI GGCC 2 cut(s) 203, 901
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 671
BsnI GGCC 2 cut(s) 203, 901
Bsp1286I GDGCHC 2 cut(s) 32, 252
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 2 cut(s) 772, 943
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 686, 848, 902, 904
BspANI GGCC 2 cut(s) 203, 901
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 904
BspLI GGNNCC 2 cut(s) 219, 319
BspPI GGATC 1 cut(s) 951
BspT107I GGYRCC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 873
BssECI CCNNGG 2 cut(s) 333, 902
BssMI GATC 2 cut(s) 772, 943
BssSI CACGAG 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 639
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 1 cut(s) 437
BstDSI CCRYGG 2 cut(s) 333, 902
BstF5I GGATG 1 cut(s) 16
BstFNI CGCG 1 cut(s) 904
BstKTI GATC 2 cut(s) 775, 946
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 2 cut(s) 772, 943
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 238
BstSLI GKGCMC 2 cut(s) 32, 252
BstUI CGCG 1 cut(s) 904
BstV1I GCAGC 2 cut(s) 369, 388
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 2 cut(s) 203, 901
BtgI CCRYGG 2 cut(s) 333, 902
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 406
Cfr42I CCGCGG 1 cut(s) 905
Csp6I GTAC 1 cut(s) 270
CviAII CATG 5 cut(s) 334, 374, 433, 461, 796
CviJI RGCY 8 cut(s) 95, 203, 360, 401, 701, 831, 854, 901
CviKI_1 RGCY 8 cut(s) 95, 203, 360, 401, 701, 831, 854, 901
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 1 cut(s) 437
DpnI GATC 2 cut(s) 774, 945
DpnII GATC 2 cut(s) 772, 943
DraI TTTAAA 2 cut(s) 198, 631
EciI GGCGGA 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 1039
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 5 cut(s) 337, 377, 436, 464, 799
FatI CATG 5 cut(s) 333, 373, 432, 460, 795
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 3 cut(s) 358, 402, 902
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 358, 402, 902
FspBI CTAG 3 cut(s) 174, 708, 791
GluI GCNGC 3 cut(s) 358, 402, 902
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 2 cut(s) 203, 901
HapII CCGG 1 cut(s) 8
Hin1II CATG 5 cut(s) 337, 377, 436, 464, 799
HinfI GANTC 2 cut(s) 464, 991
HpaII CCGG 1 cut(s) 8
Hpy166II GTNNAC 3 cut(s) 30, 642, 1003
Hpy188I TCNGA 1 cut(s) 745
Hpy188III TCNNGA 7 cut(s) 8, 347, 492, 812, 926, 947, 995
Hpy8I GTNNAC 3 cut(s) 30, 642, 1003
HpyAV CCTTC 2 cut(s) 443, 676
HpyCH4III ACNGT 1 cut(s) 639
HpyCH4V TGCA 5 cut(s) 30, 300, 408, 593, 1037
HpyF3I CTNAG 1 cut(s) 437
Hsp92II CATG 5 cut(s) 337, 377, 436, 464, 799
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 772
KspI CCGCGG 1 cut(s) 905
Kzo9I GATC 2 cut(s) 772, 943
LmnI GCTCC 1 cut(s) 746
LpnPI CCDG 6 cut(s) 21, 225, 252, 768, 777, 825
Lsp1109I GCAGC 2 cut(s) 369, 388
LweI GCATC 2 cut(s) 472, 871
MaeI CTAG 3 cut(s) 174, 708, 791
MaeIII GTNAC 2 cut(s) 169, 365
MalI GATC 2 cut(s) 774, 945
MboI GATC 2 cut(s) 772, 943
MboII GAAGA 6 cut(s) 71, 95, 507, 716, 779, 812
MhlI GDGCHC 2 cut(s) 32, 252
MluCI AATT 7 cut(s) 258, 409, 422, 504, 645, 749, 867
MmeI TCCRAC 2 cut(s) 353, 1000
MnlI CCTC 9 cut(s) 236, 262, 525, 922, 934, 964, 991, 1009, 1021
Mph1103I ATGCAT 1 cut(s) 1039
MroI TCCGGA 1 cut(s) 7
MseI TTAA 4 cut(s) 104, 197, 291, 630
MslI CAYNNNNRTG 2 cut(s) 564, 981
MspA1I CMGCKG 1 cut(s) 904
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 904
NcoI CCATGG 1 cut(s) 333
NdeII GATC 2 cut(s) 772, 943
NlaIII CATG 5 cut(s) 337, 377, 436, 464, 799
NlaIV GGNNCC 2 cut(s) 219, 319
NsiI ATGCAT 1 cut(s) 1039
NspI RCATGY 1 cut(s) 436
PceI AGGCCT 1 cut(s) 203
PfeI GAWTC 2 cut(s) 464, 991
PkrI GCNGC 3 cut(s) 359, 403, 903
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 219, 319
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 2 cut(s) 564, 981
SacII CCGCGG 1 cut(s) 905
SaqAI TTAA 4 cut(s) 104, 197, 291, 630
SatI GCNGC 3 cut(s) 358, 402, 902
Sau3AI GATC 2 cut(s) 772, 943
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 2 cut(s) 32, 252
SfaNI GCATC 2 cut(s) 472, 871
Sfr303I CCGCGG 1 cut(s) 905
SgrBI CCGCGG 1 cut(s) 905
SmiMI CAYNNNNRTG 2 cut(s) 564, 981
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
SpeI ACTAGT 1 cut(s) 173
Sse9I AATT 7 cut(s) 258, 409, 422, 504, 645, 749, 867
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 4 cut(s) 686, 848, 902, 904
SspI AATATT 1 cut(s) 527
SspMI CTAG 3 cut(s) 174, 708, 791
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 639
TaqI TCGA 3 cut(s) 81, 346, 414
TasI AATT 7 cut(s) 258, 409, 422, 504, 645, 749, 867
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 904
TfiI GAWTC 2 cut(s) 464, 991
Tru1I TTAA 4 cut(s) 104, 197, 291, 630
Tru9I TTAA 4 cut(s) 104, 197, 291, 630
TseI GCWGC 2 cut(s) 357, 401
TspDTI ATGAA 4 cut(s) 138, 762, 771, 819
VneI GTGCAC 1 cut(s) 28
XapI RAATTY 2 cut(s) 422, 749
XceI RCATGY 1 cut(s) 436
XspI CTAG 3 cut(s) 174, 708, 791
Zsp2I ATGCAT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.