Rw3G020960
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000697
Physical Location & Seq
Forward (+)
25045001 .. 25046299
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020960.1

Sequence Viewer

Length: 909 bp
ATGTATCCGGATGTCTTTCTAAAGTTGTGCACTATTATTATAGAGAGAACTGCTTTGGAAGATACAAGATATATTTGTGTCGAAGAAAGGGTAGCTACATTCTTAATGATTGTGGGGCATAATGATCGATATTGTAATGTTCGTCATAGATTTGGTCGCTCACATTTCGCAACTAGTCAAAACTTCAACAAAGTTTTAAAGGCCTTGAACACTATAGCACCACAAATGATGGTCAAACCTGGAGGTGTGCCCTCTAAAATTAGGGAAAGTACAAGGTTTTACCCTTATTTTAAGGATTGCATTGGTGCTATTGATGGAACCCACATTCCAGCCATGGTAAAAGGTCGAGAAGTAAGCAGCTATCGTAACCGTCATGGTATTCAATCTCAAAATGTTTTGGCTGCTTGCAATTTCGATTTGGAATTCATATACGTGCTTAGTGGGTGGGAAGGAAAATATTTTCTCGTGGATTGTGGATTTGCTAATCGACGCCAATTTTTAGCTCCACTACGAGGTGTCCGATATCATCTGAAAGATTTTGGTGGTCAAGGTTGCCACCCCAGAAATGCAAGTGAGATATTTGGTATCTTTAAATCACGGTTCACAATTTTCAAAATCGCACCTCCCTTCCCATTTGAGACACAAGCGGAGTTAGTGTTAGCTTGTGTTGGACTACATAACTTTCTTCTCAAAGAATGTCGCTCCGATGAATTTCCCGTTGAACCAGAAGATGATCAATCTTCATCATATCTAGACATGGAAGATGAAAATCTTGAACTACTTTCTCAAAGCCAACAACAACAAAGAGCGGAAGCTAATGCTGGGAGAATTAGCATTGCTGATGCTATGTGGAATGATAGGCCGCGGAATGATGATAATGGAAATCAAGAGGATAACAATGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

302

Amino Acids

34.61

Weight (kDa)

5.94

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 69 3.6e-17 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 809
AccII CGCG 1 cut(s) 865
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 4 cut(s) 647, 809, 863, 865
AcsI RAATTY 2 cut(s) 422, 710
AcyI GRCGYC 1 cut(s) 490
AfaI GTAC 1 cut(s) 271
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 6 cut(s) 187, 208, 383, 613, 722, 776
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 1 cut(s) 238
AloI GAACNNNNNNTCC 2 cut(s) 310, 342
AluBI AGCT 5 cut(s) 95, 360, 503, 662, 815
AluI AGCT 5 cut(s) 95, 360, 503, 662, 815
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 632
Alw44I GTGCAC 1 cut(s) 28
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 201, 860
ApaLI GTGCAC 1 cut(s) 28
ApeKI GCWGC 2 cut(s) 357, 401
ApoI RAATTY 2 cut(s) 422, 710
BaeGI GKGCMC 2 cut(s) 32, 252
BauI CACGAG 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 369, 388
BccI CCATC 2 cut(s) 223, 308
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 1 cut(s) 240
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 1 cut(s) 632
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 2 cut(s) 174, 752
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 3 cut(s) 358, 402, 863
BlsI GCNGC 3 cut(s) 359, 403, 864
Bme1390I CCNGG 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 832
BpmI CTGGAG 1 cut(s) 261
Bsa29I ATCGAT 1 cut(s) 127
BsaAI YACGTR 1 cut(s) 433
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 333, 863
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 834
Bse8I GATNNNNATC 1 cut(s) 768
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 333, 863
BseGI GGATG 1 cut(s) 16
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMI GCAATG 1 cut(s) 834
BseSI GKGCMC 2 cut(s) 32, 252
BseXI GCAGC 2 cut(s) 369, 388
BseYI CCCAGC 1 cut(s) 821
Bsh1236I CGCG 1 cut(s) 865
BshFI GGCC 2 cut(s) 203, 862
BshVI ATCGAT 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 632
BsnI GGCC 2 cut(s) 203, 862
Bsp1286I GDGCHC 2 cut(s) 32, 252
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 2 cut(s) 124, 733
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 647, 809, 863, 865
BspANI GGCC 2 cut(s) 203, 862
BspDI ATCGAT 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 865
BspLI GGNNCC 1 cut(s) 319
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 834
BssECI CCNNGG 2 cut(s) 333, 863
BssMI GATC 2 cut(s) 124, 733
BssNI GRCGYC 1 cut(s) 490
BssSI CACGAG 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 371, 600
BstACI GRCGYC 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 1 cut(s) 437
BstDSI CCRYGG 2 cut(s) 333, 863
BstF5I GGATG 1 cut(s) 16
BstFNI CGCG 1 cut(s) 865
BstKTI GATC 2 cut(s) 127, 736
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 2 cut(s) 124, 733
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 2 cut(s) 32, 252
BstUI CGCG 1 cut(s) 865
BstV1I GCAGC 2 cut(s) 369, 388
Bsu15I ATCGAT 1 cut(s) 127
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 2 cut(s) 203, 862
BsuTUI ATCGAT 1 cut(s) 127
BtgI CCRYGG 2 cut(s) 333, 863
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 406
Cfr42I CCGCGG 1 cut(s) 866
ClaI ATCGAT 1 cut(s) 127
CseI GACGC 1 cut(s) 498
Csp6I GTAC 1 cut(s) 270
CviAII CATG 3 cut(s) 334, 374, 757
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 1 cut(s) 437
DpnI GATC 2 cut(s) 126, 735
DpnII GATC 2 cut(s) 124, 733
DraI TTTAAA 2 cut(s) 198, 592
Eco130I CCWWGG 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 203
Eco32I GATATC 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 524
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 3 cut(s) 337, 377, 760
FatI CATG 3 cut(s) 333, 373, 756
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 3 cut(s) 358, 402, 863
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 358, 402, 863
FspBI CTAG 2 cut(s) 174, 752
GluI GCNGC 3 cut(s) 358, 402, 863
GsaI CCCAGC 1 cut(s) 825
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 2 cut(s) 203, 862
HapII CCGG 1 cut(s) 8
HgaI GACGC 1 cut(s) 498
Hin1I GRCGYC 1 cut(s) 490
Hin1II CATG 3 cut(s) 337, 377, 760
HpaII CCGG 1 cut(s) 8
Hpy166II GTNNAC 2 cut(s) 30, 603
Hpy188I TCNGA 3 cut(s) 521, 531, 706
Hpy188III TCNNGA 5 cut(s) 8, 347, 752, 773, 887
Hpy8I GTNNAC 2 cut(s) 30, 603
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 443, 637
HpyCH4III ACNGT 2 cut(s) 371, 600
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 4 cut(s) 30, 300, 408, 569
HpyF3I CTNAG 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 432
Hsp92I GRCGYC 1 cut(s) 490
Hsp92II CATG 3 cut(s) 337, 377, 760
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 733
KspI CCGCGG 1 cut(s) 866
Kzo9I GATC 2 cut(s) 124, 733
LmnI GCTCC 2 cut(s) 508, 707
LpnPI CCDG 7 cut(s) 21, 225, 252, 342, 574, 738, 807
Lsp1109I GCAGC 2 cut(s) 369, 388
LweI GCATC 1 cut(s) 832
MaeI CTAG 2 cut(s) 174, 752
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 365
MalI GATC 2 cut(s) 126, 735
MbiI CCGCTC 1 cut(s) 809
MboI GATC 2 cut(s) 124, 733
MboII GAAGA 6 cut(s) 71, 95, 677, 732, 740, 773
MhlI GDGCHC 2 cut(s) 32, 252
MluCI AATT 7 cut(s) 258, 409, 422, 494, 606, 710, 828
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 6 cut(s) 236, 262, 506, 633, 883, 895
MroI TCCGGA 1 cut(s) 7
MseI TTAA 5 cut(s) 104, 197, 291, 591, 907
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 865
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 865
NcoI CCATGG 1 cut(s) 333
NdeII GATC 2 cut(s) 124, 733
NlaIII CATG 3 cut(s) 337, 377, 760
NlaIV GGNNCC 1 cut(s) 319
PceI AGGCCT 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PkrI GCNGC 3 cut(s) 359, 403, 864
Ppu21I YACGTR 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 238
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 319
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 1 cut(s) 431
SacII CCGCGG 1 cut(s) 866
SaqAI TTAA 5 cut(s) 104, 197, 291, 591, 907
SatI GCNGC 3 cut(s) 358, 402, 863
Sau3AI GATC 2 cut(s) 124, 733
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 2 cut(s) 32, 252
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 1 cut(s) 213
Sfr303I CCGCGG 1 cut(s) 866
SgrBI CCGCGG 1 cut(s) 866
SmiMI CAYNNNNRTG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 173
Sse9I AATT 7 cut(s) 258, 409, 422, 494, 606, 710, 828
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 4 cut(s) 647, 809, 863, 865
SspI AATATT 1 cut(s) 458
SspMI CTAG 2 cut(s) 174, 752
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 371, 600
TaiI ACGT 1 cut(s) 435
TaqI TCGA 5 cut(s) 81, 127, 346, 414, 487
TasI AATT 7 cut(s) 258, 409, 422, 494, 606, 710, 828
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 865
Tru1I TTAA 5 cut(s) 104, 197, 291, 591, 907
Tru9I TTAA 5 cut(s) 104, 197, 291, 591, 907
TseI GCWGC 2 cut(s) 357, 401
TspDTI ATGAA 4 cut(s) 415, 723, 732, 780
VneI GTGCAC 1 cut(s) 28
XapI RAATTY 2 cut(s) 422, 710
XbaI TCTAGA 1 cut(s) 751
XspI CTAG 2 cut(s) 174, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.