Rmu_sc0009973.1_g000001

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009973.1
OG0000697
Physical Location & Seq
Forward (+)
5455 .. 6920
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009973.1_g000001.1.cds

Sequence Viewer

Length: 1251 bp
atgtctgaaatggacatgtatggagttgatgaagaggaagaacagttttacgaggctgttaagatattattaatggcaatacaagcagtggtttatgtgttatacgaccttgtattcaacatacgtggtgaacgtattagacgtccactgactcggcgaccagtgacatcaagtggatacatatatatgcacaaaatattagacagagaccctcaaatctttagagaggtgtatagaatgtatcctgacgtttttcgaaaattatgtagcatcctaaaagtgaaaacacctttgcgggatacaagacacatttgtgttgaagaaatgctcgcaacctttctacttgttgtcggccaaaacaatcgatacagtgaagctcggctgatatttgagcgatctcatttcactgttagcaaaagtttcaacaaagtcttgaaggccctgaatacaatagcaccggagtttatggctaaacctgagtccgtgccacctaacataagagaaagtacaaggttttatccttactttaaggattgcgtcggagctatagatggcacacatattccagcaacggtagttgaacgtgaggttagcagatatcgaaatcgacatgggaatatatcacaaaatgtattagcagcttgtaactttgatttacagttcatatatgtacttagtggatgggagggttccgctcatgattcaaaagtattgaatgatgtgatttctagacgaaatggactcaaagtgccaccaggggactggggatttccaaatcgacgccggtttttagctccatttcgaggtactggatatcatctcaaagattttggtggtgaaggaaaccatcctgtcaatgcaattgagttattcaatcttcgtcatgcttccttgaggaatgtaattgagagaatatttggaatatttaagtcgcgtttcacaatcttcaaatcagcaccaccattttcatataagacacaagcagagctagttttggcttgtgcaggactgcacaattttcttcgacaggaatgccgttcagatgaatttcctcccgaaccagaagaagatccgatagacaatcacgaagataattttgaatgggatgattttcaaacccaagatcagcagagagaaaatgctaatgaatggagaatgagtattgctactcatatgtggacagatgtccaatcaaatgccaacaatgaaaacaataacaatgaagaaagtgaaaatgaaggagaaggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

416

Amino Acids

48.41

Weight (kDa)

5.9

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 695
AccII CGCG 1 cut(s) 934
AciI CCGC 2 cut(s) 295, 693
AclWI GGATC 1 cut(s) 1064
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 1 cut(s) 1046
AcyI GRCGYC 2 cut(s) 142, 781
AfaI GTAC 3 cut(s) 508, 672, 808
AfiI CCNNNNNNNGG 1 cut(s) 803
AflIII ACRYGT 1 cut(s) 15
AjnI CCWGG 1 cut(s) 754
AleI CACNNNNGTG 1 cut(s) 312
AluBI AGCT 5 cut(s) 377, 545, 641, 794, 988
AluI AGCT 5 cut(s) 377, 545, 641, 794, 988
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 1064
AoxI GGCC 2 cut(s) 352, 438
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 1 cut(s) 1046
AseI ATTAAT 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 140, 848
AsuII TTCGAA 1 cut(s) 256
BbvI GCAGC 1 cut(s) 650
BccI CCATC 3 cut(s) 545, 675, 855
BceAI ACGGC 1 cut(s) 1020
BcgI CGANNNNNNTGC 2 cut(s) 1014, 1048
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 3 cut(s) 170, 252, 292
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 729, 989
BfmI CTRYAG 1 cut(s) 546
BfuI GTATCC 3 cut(s) 170, 252, 292
BisI GCNGC 1 cut(s) 639
BlsI GCNGC 1 cut(s) 640
Bme1390I CCNGG 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 691
BmrFI CCNGG 1 cut(s) 756
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 772
BoxI GACNNNNGTC 1 cut(s) 1184
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 913
Bsa29I ATCGAT 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 2 cut(s) 142, 781
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 755
BsaWI WCCGGW 1 cut(s) 457
BsaXI ACNNNNNCTCC 2 cut(s) 1144, 1174
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse118I RCCGGY 1 cut(s) 783
Bse1I ACTGG 3 cut(s) 161, 767, 814
BseBI CCWGG 1 cut(s) 756
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 755
BseGI GGATG 4 cut(s) 270, 686, 847, 1111
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 3 cut(s) 161, 767, 814
BseXI GCAGC 1 cut(s) 650
BsgI GTGCAG 2 cut(s) 995, 1023
Bsh1236I CGCG 1 cut(s) 934
BshFI GGCC 2 cut(s) 354, 440
BshVI ATCGAT 1 cut(s) 364
BsiSI CCGG 2 cut(s) 458, 784
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 803
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 773
BsmI GAATGC 1 cut(s) 1037
BsnI GGCC 2 cut(s) 354, 440
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 3 cut(s) 395, 1069, 1123
BspACI CCGC 2 cut(s) 295, 693
BspANI GGCC 2 cut(s) 354, 440
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 1 cut(s) 364
BspFNI CGCG 1 cut(s) 934
BspHI TCATGA 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 691
BspPI GGATC 1 cut(s) 1064
BspT104I TTCGAA 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 695
BsrFI RCCGGY 1 cut(s) 783
BsrI ACTGG 3 cut(s) 161, 767, 814
BssAI RCCGGY 1 cut(s) 783
BssECI CCNNGG 1 cut(s) 755
BssMI GATC 3 cut(s) 395, 1069, 1123
BssNI GRCGYC 2 cut(s) 142, 781
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 45, 371, 409, 574, 660
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 2 cut(s) 142, 781
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 477, 674
BstF5I GGATG 4 cut(s) 270, 686, 847, 1111
BstFNI CGCG 1 cut(s) 934
BstKTI GATC 3 cut(s) 398, 1072, 1126
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 3 cut(s) 395, 1069, 1123
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 1 cut(s) 19
BstPAI GACNNNNGTC 1 cut(s) 1184
BstSCI CCNGG 1 cut(s) 754
BstSFI CTRYAG 1 cut(s) 546
BstUI CGCG 1 cut(s) 934
BstV1I GCAGC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 1069
BstXI CCANNNNNNTGG 1 cut(s) 762
BstYI RGATCY 1 cut(s) 1069
Bsu15I ATCGAT 1 cut(s) 364
BsuI GTATCC 3 cut(s) 170, 252, 292
BsuRI GGCC 2 cut(s) 354, 440
BsuTUI ATCGAT 1 cut(s) 364
BtsCI GGATG 4 cut(s) 270, 686, 847, 1111
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 5 cut(s) 93, 146, 168, 376, 405
Cac8I GCNNGC 1 cut(s) 330
CciI TCATGA 1 cut(s) 697
Cfr10I RCCGGY 1 cut(s) 783
Cfr13I GGNCC 1 cut(s) 439
ClaI ATCGAT 1 cut(s) 364
CseI GACGC 2 cut(s) 526, 789
Csp6I GTAC 3 cut(s) 507, 671, 807
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 4 cut(s) 16, 611, 698, 884
CviQI GTAC 3 cut(s) 507, 671, 807
DdeI CTNAG 2 cut(s) 477, 674
DpnI GATC 3 cut(s) 397, 1071, 1125
DpnII GATC 3 cut(s) 395, 1069, 1123
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 2 cut(s) 599, 815
EcoO109I RGGNCCY 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 2 cut(s) 599, 815
FaeI CATG 4 cut(s) 19, 614, 701, 887
FaqI GGGAC 1 cut(s) 773
FatI CATG 4 cut(s) 15, 610, 697, 883
FauI CCCGC 1 cut(s) 288
FauNDI CATATG 1 cut(s) 1173
Fnu4HI GCNGC 1 cut(s) 639
FokI GGATG 4 cut(s) 257, 693, 834, 1118
Fsp4HI GCNGC 1 cut(s) 639
FspBI CTAG 2 cut(s) 729, 989
GluI GCNGC 1 cut(s) 639
HaeIII GGCC 2 cut(s) 354, 440
HapII CCGG 2 cut(s) 458, 784
HgaI GACGC 2 cut(s) 526, 789
Hin1I GRCGYC 2 cut(s) 142, 781
Hin1II CATG 4 cut(s) 19, 614, 701, 887
HinfI GANTC 4 cut(s) 151, 479, 701, 741
HpaII CCGG 2 cut(s) 458, 784
HphI GGTGA 2 cut(s) 140, 848
Hpy166II GTNNAC 3 cut(s) 131, 146, 1179
Hpy188I TCNGA 4 cut(s) 7, 542, 1042, 1074
Hpy188III TCNNGA 6 cut(s) 245, 433, 698, 729, 1055, 1085
Hpy8I GTNNAC 3 cut(s) 131, 146, 1179
Hpy99I CGWCG 2 cut(s) 542, 783
HpyAV CCTTC 4 cut(s) 430, 833, 1232, 1238
HpyCH4III ACNGT 5 cut(s) 45, 371, 409, 574, 660
HpyCH4IV ACGT 5 cut(s) 124, 133, 142, 249, 583
HpyCH4V TGCA 4 cut(s) 190, 860, 1004, 1012
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 477, 674
HpySE526I ACGT 5 cut(s) 124, 133, 142, 249, 583
Hsp92I GRCGYC 2 cut(s) 142, 781
Hsp92II CATG 4 cut(s) 19, 614, 701, 887
Kzo9I GATC 3 cut(s) 395, 1069, 1123
LmnI GCTCC 2 cut(s) 542, 799
Lsp1109I GCAGC 1 cut(s) 650
LweI GCATC 1 cut(s) 279
MaeI CTAG 2 cut(s) 729, 989
MaeII ACGT 5 cut(s) 124, 133, 142, 249, 583
MaeIII GTNAC 2 cut(s) 163, 644
MalI GATC 3 cut(s) 397, 1071, 1125
MbiI CCGCTC 1 cut(s) 695
MboI GATC 3 cut(s) 395, 1069, 1123
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 1069
MluCI AATT 6 cut(s) 260, 861, 903, 1015, 1046, 1093
MlyI GAGTC 3 cut(s) 145, 488, 735
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 9 cut(s) 28, 46, 220, 222, 580, 679, 797, 888, 1062
MseI TTAA 4 cut(s) 60, 71, 528, 927
MslI CAYNNNNRTG 2 cut(s) 185, 312
MspI CCGG 2 cut(s) 458, 784
MspR9I CCNGG 1 cut(s) 756
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 1037
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 1 cut(s) 934
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeI CATATG 1 cut(s) 1173
NdeII GATC 3 cut(s) 395, 1069, 1123
NlaIII CATG 4 cut(s) 19, 614, 701, 887
NlaIV GGNNCC 1 cut(s) 691
NmeAIII GCCGAG 2 cut(s) 133, 358
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 256
OliI CACNNNNGTG 1 cut(s) 312
PagI TCATGA 1 cut(s) 697
PciI ACATGT 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 139
PctI GAATGC 1 cut(s) 1037
PfeI GAWTC 1 cut(s) 701
PkrI GCNGC 1 cut(s) 640
PleI GAGTC 3 cut(s) 145, 487, 735
PpsI GAGTC 3 cut(s) 145, 487, 735
Ppu21I YACGTR 1 cut(s) 125
PscI ACATGT 1 cut(s) 15
PshAI GACNNNNGTC 1 cut(s) 1184
PshBI ATTAAT 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 1 cut(s) 691
PspPI GGNCC 1 cut(s) 439
PsuI RGATCY 1 cut(s) 1069
RsaI GTAC 3 cut(s) 508, 672, 808
RsaNI GTAC 3 cut(s) 507, 671, 807
RseI CAYNNNNRTG 2 cut(s) 185, 312
SaqAI TTAA 4 cut(s) 60, 71, 528, 927
SatI GCNGC 1 cut(s) 639
Sau3AI GATC 3 cut(s) 395, 1069, 1123
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 3 cut(s) 145, 488, 735
ScrFI CCNGG 1 cut(s) 756
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 185, 312
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
Sse9I AATT 6 cut(s) 260, 861, 903, 1015, 1046, 1093
SsiI CCGC 2 cut(s) 295, 693
SspI AATATT 3 cut(s) 198, 915, 924
SspMI CTAG 2 cut(s) 729, 989
StyD4I CCNGG 1 cut(s) 754
TaaI ACNGT 5 cut(s) 45, 371, 409, 574, 660
TaiI ACGT 5 cut(s) 127, 136, 145, 252, 586
TaqI TCGA 7 cut(s) 256, 364, 601, 607, 778, 802, 1024
TasI AATT 6 cut(s) 260, 861, 903, 1015, 1046, 1093
TatI WGTACW 2 cut(s) 506, 670
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 4 cut(s) 60, 71, 528, 927
Tru9I TTAA 4 cut(s) 60, 71, 528, 927
TscAI CASTG 5 cut(s) 93, 153, 168, 376, 412
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 638
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 8 cut(s) 45, 652, 957, 1059, 1161, 1221, 1236, 1251
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 5 cut(s) 93, 153, 168, 376, 412
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 1046
XbaI TCTAGA 1 cut(s) 728
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 759
XspI CTAG 2 cut(s) 729, 989
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.