Rw0G006340
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00245
OG0000697
Physical Location & Seq
Reverse (-)
61155 .. 63898
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006340.1

Sequence Viewer

Length: 1653 bp
ATGAGAGATTCACAAGGGAATGGTAAAAGAAAGGATTACAAAACTTGGAACACTGAAGAAAGCAACGTTTTGCTTCAACTTATGGTTGAAGGCGCCAAACTTGGATTTCGTGATATTAATGGTCTTATGCGCCATAGTTCTGGATTTGGGTGGGATCCCATCACAAAAAGATTCACTGCTGATGATGAAGTATGGGCAGATTATTTGAAGTCACATCCAACGCATGGGCACTTTCGGTCAGAAACTTTTCCAGACTATGAGGACTTGAAAATTGCAGTTGGAAATGGAACTGCAACTGGAATGGGCTCAATCAGTCTAGGTGATGATACAGATGCAACAACATTTGGAGCGGAGGAAAATGAATCTTGGGGAATTGGTGGCATAGATGGATTAGTTTTTGATCGAAATACTAACATGTTCGTACAAAGTGAAAATGAGTCATCACAACAACCCTCCCAAGGTACCAGTATAGATGCTCCAGCTCCACCACATAGCAGGACTCAAGGAAAACGGAGTAGAACTGACTATGAAAATAATAGTTCTAAAAACTTATCAAGTGGCATTGGTAAAATTGTCACAAGTTTTGATAAAATTTATGGCTTAATGGAGAAGAGAGAATCAAGAGAGAGTGACCTTTTGGATGCTATGAAGGAGACTCCAGGATTAACTGATGAAGCTTGCTTTATGGCTCTTGATTTGCTTAAACTAAAGCAAACAATGTCTGAAATGGACATGTATGGAGCTGATGAAGAGGAAGAACAGTTTTACGAGGCTGTTAAGATATTATTAATGGCAATACAAGCAGTGGTTTATGTGTTATACGACCTTGTATTCAGCATACGTGGTGAACGTAAGCAATGCAACTTTTATGGATGGTTATTTATTTCATTGTACAGTAATGAAGATATTATTTCATTTTTCTTTTCAACTATTTTATTTTTTTCATTGTTTCAGGATTGCGTCGGAGCTATAGATGGCACACATATTCCAGCAACGGTAGTTGGACGTGAGGTTAGCAGATATCGAAATCGACATGGGAAGATATCACAAAATGTATTAGCAGCTTGTAACTTTGATTTACAGTTCACATATGTAATTAGTGGATGGGAGGGTTCCGCTCATGATTCAAAAGTATTGAATGATGCGATTTCTAGACGAAATGGACTCAAAGTGCCACCAGGTAAATATTACTTAGGGGACTGCGGATTTCCAAATCGACGCCGGTTTTTAGCTCCATTTCGAGGTACTCGATATCATCTCAAAGATTTTGGTGGTGAAGGAAACCATCCTGTCAATGCAATTGAGTTATTCAATCTTCGTCATGCTTCCTTGAGGAATGTAATTGAGAGAATATTTGGAATATTTAAGTCGCGTTTCACAATCTTCAAATCAGCACCACCATTTTCATATAAGACACAAGCAGAGCTAGTTTTGGCTTGTGCAGGACTGCACAATTTTCTTCGACAGGAATGCCGTTCAGATGAATTTCCTCCCGAACCAGAAGAAGATCCGATGGACAATCACGAAGATAATTTTGAATGGGATGATTTTCAAACCCAAGATCATATTGCTACTCATATGTGGACAGATGCCCAACCAAATGCCAACAATGAAAACAATAACAATGAAAGTGAAAATGAAGGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

550

Amino Acids

62.34

Weight (kDa)

4.99

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_1 PF01609 318 - 463 1.6e-06 Transposase DDE domain
DDE_Tnp_4 PF13359 324 - 485 3.6e-26 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 461
AccB1I GGYRCC 2 cut(s) 92, 461
AccB7I CCANNNNNTGG 1 cut(s) 224
AccBSI CCGCTC 2 cut(s) 350, 1118
AccII CGCG 1 cut(s) 1372
AciI CCGC 3 cut(s) 350, 1116, 1203
AclI AACGTT 1 cut(s) 66
AclWI GGATC 3 cut(s) 149, 162, 1502
AcsI RAATTY 2 cut(s) 591, 1484
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 2 cut(s) 93, 1219
AfaI GTAC 4 cut(s) 423, 463, 893, 1246
AfiI CCNNNNNNNGG 3 cut(s) 224, 458, 1241
AflIII ACRYGT 2 cut(s) 414, 732
AjiI CACGTC 1 cut(s) 1007
AjnI CCWGG 2 cut(s) 658, 1177
AluBI AGCT 7 cut(s) 482, 677, 743, 968, 1064, 1232, 1426
AluI AGCT 7 cut(s) 482, 677, 743, 968, 1064, 1232, 1426
Alw26I GTCTC 1 cut(s) 647
AlwI GGATC 3 cut(s) 149, 162, 1502
ApeKI GCWGC 1 cut(s) 1061
ApoI RAATTY 2 cut(s) 591, 1484
AseI ATTAAT 2 cut(s) 117, 788
Asp700I GAANNNNTTC 1 cut(s) 246
Asp718I GGTACC 1 cut(s) 461
AspLEI GCGC 2 cut(s) 95, 132
AsuHPI GGTGA 3 cut(s) 332, 857, 1286
BaeGI GKGCMC 1 cut(s) 231
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 2 cut(s) 92, 461
BanII GRGCYC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 1073
BccI CCATC 7 cut(s) 167, 380, 867, 968, 1098, 1293, 1507
BceAI ACGGC 1 cut(s) 1458
BcgI CGANNNNNNTGC 2 cut(s) 1452, 1486
BciT130I CCWGG 2 cut(s) 660, 1179
BcoDI GTCTC 1 cut(s) 647
BfaI CTAG 3 cut(s) 317, 1152, 1427
BfmI CTRYAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 1 cut(s) 1062
BlsI GCNGC 1 cut(s) 1063
Bme1390I CCNGG 2 cut(s) 660, 1179
BmgBI CACGTC 1 cut(s) 1007
BmiI GGNNCC 4 cut(s) 94, 156, 463, 1114
BmrFI CCNGG 2 cut(s) 660, 1179
BmsI GCATC 5 cut(s) 322, 463, 631, 1132, 1579
BpmI CTGGAG 2 cut(s) 462, 642
BpuEI CTTGAG 2 cut(s) 486, 1351
BsaAI YACGTR 1 cut(s) 842
BsaHI GRCGYC 2 cut(s) 93, 1219
BsaJI CCNNGG 1 cut(s) 457
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 458, 1241
Bse118I RCCGGY 1 cut(s) 1221
Bse1I ACTGG 2 cut(s) 301, 465
Bse3DI GCAATG 1 cut(s) 863
BseBI CCWGG 2 cut(s) 660, 1179
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 6 cut(s) 214, 646, 878, 1109, 1285, 1549
BseLI CCNNNNNNNGG 3 cut(s) 224, 458, 1241
BseMI GCAATG 1 cut(s) 863
BseNI ACTGG 2 cut(s) 301, 465
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 1 cut(s) 1073
BsgI GTGCAG 2 cut(s) 1433, 1461
Bsh1236I CGCG 1 cut(s) 1372
BshNI GGYRCC 2 cut(s) 92, 461
BsiSI CCGG 1 cut(s) 1222
BslFI GGGAC 1 cut(s) 1211
BslI CCNNNNNNNGG 3 cut(s) 224, 458, 1241
BsmAI GTCTC 1 cut(s) 647
BsmFI GGGAC 1 cut(s) 1211
BsmI GAATGC 1 cut(s) 1475
Bsp1286I GDGCHC 2 cut(s) 231, 308
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 4 cut(s) 154, 400, 1507, 1561
BspACI CCGC 3 cut(s) 350, 1116, 1203
BspFNI CGCG 1 cut(s) 1372
BspHI TCATGA 1 cut(s) 1120
BspLI GGNNCC 4 cut(s) 94, 156, 463, 1114
BspPI GGATC 3 cut(s) 149, 162, 1502
BspT107I GGYRCC 2 cut(s) 92, 461
BsrBI CCGCTC 2 cut(s) 350, 1118
BsrDI GCAATG 1 cut(s) 863
BsrFI RCCGGY 1 cut(s) 1221
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 2 cut(s) 301, 465
BssAI RCCGGY 1 cut(s) 1221
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 4 cut(s) 154, 400, 1507, 1561
BssNI GRCGYC 2 cut(s) 93, 1219
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 660, 1179
Bst4CI ACNGT 4 cut(s) 762, 896, 997, 1083
Bst6I CTCTTC 2 cut(s) 605, 744
BstACI GRCGYC 2 cut(s) 93, 1219
BstAUI TGTACA 1 cut(s) 891
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 1 cut(s) 1192
BstF5I GGATG 6 cut(s) 214, 646, 878, 1109, 1285, 1549
BstFNI CGCG 1 cut(s) 1372
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 132
BstKTI GATC 4 cut(s) 157, 403, 1510, 1564
BstMAI GTCTC 1 cut(s) 647
BstMBI GATC 4 cut(s) 154, 400, 1507, 1561
BstMWI GCNNNNNNNGC 1 cut(s) 800
BstNI CCWGG 2 cut(s) 660, 1179
BstNSI RCATGY 2 cut(s) 418, 736
BstSCI CCNGG 2 cut(s) 658, 1177
BstSFI CTRYAG 1 cut(s) 969
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 1 cut(s) 1372
BstV1I GCAGC 1 cut(s) 1073
BstX2I RGATCY 2 cut(s) 154, 1507
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 2 cut(s) 154, 1507
BtrI CACGTC 1 cut(s) 1007
BtsCI GGATG 6 cut(s) 214, 646, 878, 1109, 1285, 1549
BtsI GCAGTG 2 cut(s) 174, 810
BtsIMutI CAGTG 3 cut(s) 51, 174, 810
Cac8I GCNNGC 1 cut(s) 679
CciI TCATGA 1 cut(s) 1120
CfoI GCGC 2 cut(s) 95, 132
Cfr10I RCCGGY 1 cut(s) 1221
CseI GACGC 2 cut(s) 949, 1227
CsiI ACCWGGT 1 cut(s) 1177
Csp6I GTAC 4 cut(s) 422, 462, 892, 1245
CviAII CATG 6 cut(s) 224, 415, 733, 1034, 1121, 1322
CviQI GTAC 4 cut(s) 422, 462, 892, 1245
DdeI CTNAG 1 cut(s) 1192
DinI GGCGCC 1 cut(s) 94
DpnI GATC 4 cut(s) 156, 402, 1509, 1563
DpnII GATC 4 cut(s) 154, 400, 1507, 1561
Eam1104I CTCTTC 2 cut(s) 605, 744
EarI CTCTTC 2 cut(s) 605, 744
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 308
Eco32I GATATC 3 cut(s) 1022, 1044, 1253
Eco57I CTGAAG 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 658, 1177
EcoRV GATATC 3 cut(s) 1022, 1044, 1253
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 308
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 6 cut(s) 227, 418, 736, 1037, 1124, 1325
FaqI GGGAC 1 cut(s) 1211
FatI CATG 6 cut(s) 223, 414, 732, 1033, 1120, 1321
FauNDI CATATG 2 cut(s) 1090, 1578
Fnu4HI GCNGC 1 cut(s) 1062
FokI GGATG 6 cut(s) 201, 653, 885, 1116, 1272, 1556
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 1062
FspBI CTAG 3 cut(s) 317, 1152, 1427
GlaI GCGC 2 cut(s) 94, 131
GluI GCNGC 1 cut(s) 1062
GsuI CTGGAG 2 cut(s) 462, 642
HaeII RGCGCY 1 cut(s) 96
HapII CCGG 1 cut(s) 1222
HgaI GACGC 2 cut(s) 949, 1227
HhaI GCGC 2 cut(s) 95, 132
Hin1I GRCGYC 2 cut(s) 93, 1219
Hin1II CATG 6 cut(s) 227, 418, 736, 1037, 1124, 1325
Hin6I GCGC 2 cut(s) 93, 130
HinP1I GCGC 2 cut(s) 93, 130
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 9 cut(s) 8, 171, 362, 437, 499, 617, 655, 1124, 1164
HpaII CCGG 1 cut(s) 1222
HphI GGTGA 3 cut(s) 332, 857, 1286
Hpy166II GTNNAC 3 cut(s) 848, 1086, 1584
Hpy188I TCNGA 5 cut(s) 241, 724, 965, 1480, 1512
Hpy8I GTNNAC 3 cut(s) 848, 1086, 1584
Hpy99I CGWCG 2 cut(s) 965, 1221
HpyAV CCTTC 4 cut(s) 83, 643, 1271, 1634
HpyCH4III ACNGT 4 cut(s) 762, 896, 997, 1083
HpyCH4IV ACGT 4 cut(s) 66, 841, 850, 1006
HpyCH4V TGCA 7 cut(s) 275, 293, 335, 861, 1298, 1442, 1450
HpyF10VI GCNNNNNNNGC 1 cut(s) 800
HpyF3I CTNAG 1 cut(s) 1192
HpySE526I ACGT 4 cut(s) 66, 841, 850, 1006
Hsp92I GRCGYC 2 cut(s) 93, 1219
Hsp92II CATG 6 cut(s) 227, 418, 736, 1037, 1124, 1325
HspAI GCGC 2 cut(s) 93, 130
KasI GGCGCC 1 cut(s) 92
KpnI GGTACC 1 cut(s) 465
Kzo9I GATC 4 cut(s) 154, 400, 1507, 1561
LmnI GCTCC 6 cut(s) 347, 481, 487, 740, 965, 1237
Lsp1109I GCAGC 1 cut(s) 1073
LweI GCATC 5 cut(s) 322, 463, 631, 1132, 1579
MabI ACCWGGT 1 cut(s) 1177
MaeI CTAG 3 cut(s) 317, 1152, 1427
MaeII ACGT 4 cut(s) 66, 841, 850, 1006
MaeIII GTNAC 4 cut(s) 210, 574, 629, 1067
MalI GATC 4 cut(s) 156, 402, 1509, 1563
MbiI CCGCTC 2 cut(s) 350, 1118
MboI GATC 4 cut(s) 154, 400, 1507, 1561
MfeI CAATTG 1 cut(s) 1299
MflI RGATCY 2 cut(s) 154, 1507
MhlI GDGCHC 2 cut(s) 231, 308
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 4 cut(s) 446, 493, 649, 1158
MmeI TCCRAC 4 cut(s) 242, 259, 943, 982
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 7 cut(s) 117, 602, 665, 702, 777, 788, 1365
MspI CCGG 1 cut(s) 1222
MspR9I CCNGG 2 cut(s) 660, 1179
MunI CAATTG 1 cut(s) 1299
Mva1269I GAATGC 1 cut(s) 1475
MvaI CCWGG 2 cut(s) 660, 1179
MvnI CGCG 1 cut(s) 1372
MwoI GCNNNNNNNGC 1 cut(s) 800
NarI GGCGCC 1 cut(s) 93
NdeI CATATG 2 cut(s) 1090, 1578
NdeII GATC 4 cut(s) 154, 400, 1507, 1561
NlaIII CATG 6 cut(s) 227, 418, 736, 1037, 1124, 1325
NlaIV GGNNCC 4 cut(s) 94, 156, 463, 1114
NmuCI GTSAC 3 cut(s) 210, 574, 629
NspI RCATGY 2 cut(s) 418, 736
PagI TCATGA 1 cut(s) 1120
PciI ACATGT 2 cut(s) 414, 732
PcsI WCGNNNNNNNCGW 2 cut(s) 847, 1246
PctI GAATGC 1 cut(s) 1475
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 5 cut(s) 8, 171, 362, 617, 1124
PflMI CCANNNNNTGG 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 1 cut(s) 1063
PleI GAGTC 4 cut(s) 445, 493, 649, 1158
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 4 cut(s) 445, 493, 649, 1158
Ppu21I YACGTR 1 cut(s) 842
PscI ACATGT 2 cut(s) 414, 732
PshBI ATTAAT 2 cut(s) 117, 788
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 658, 1177
PspGI CCWGG 2 cut(s) 658, 1177
PspN4I GGNNCC 4 cut(s) 94, 156, 463, 1114
PsuI RGATCY 2 cut(s) 154, 1507
RsaI GTAC 4 cut(s) 423, 463, 893, 1246
RsaNI GTAC 4 cut(s) 422, 462, 892, 1245
SaqAI TTAA 7 cut(s) 117, 602, 665, 702, 777, 788, 1365
SatI GCNGC 1 cut(s) 1062
Sau3AI GATC 4 cut(s) 154, 400, 1507, 1561
SchI GAGTC 4 cut(s) 446, 493, 649, 1158
ScrFI CCNGG 2 cut(s) 660, 1179
SduI GDGCHC 2 cut(s) 231, 308
SexAI ACCWGGT 1 cut(s) 1177
SfaNI GCATC 5 cut(s) 322, 463, 631, 1132, 1579
SfcI CTRYAG 1 cut(s) 969
SfoI GGCGCC 1 cut(s) 94
SmlI CTYRAG 2 cut(s) 501, 1330
SmoI CTYRAG 2 cut(s) 501, 1330
SsiI CCGC 3 cut(s) 350, 1116, 1203
SspDI GGCGCC 1 cut(s) 92
SspI AATATT 3 cut(s) 1187, 1353, 1362
SspMI CTAG 3 cut(s) 317, 1152, 1427
StyD4I CCNGG 2 cut(s) 658, 1177
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 762, 896, 997, 1083
TaiI ACGT 4 cut(s) 69, 844, 853, 1009
TaqI TCGA 7 cut(s) 403, 1024, 1030, 1216, 1240, 1249, 1462
TaqII GACCGA 1 cut(s) 225
TatI WGTACW 1 cut(s) 891
TfiI GAWTC 5 cut(s) 8, 171, 362, 617, 1124
Tru1I TTAA 7 cut(s) 117, 602, 665, 702, 777, 788, 1365
Tru9I TTAA 7 cut(s) 117, 602, 665, 702, 777, 788, 1365
TscAI CASTG 3 cut(s) 58, 181, 810
TseFI GTSAC 3 cut(s) 210, 574, 629
TseI GCWGC 1 cut(s) 1061
Tsp45I GTSAC 3 cut(s) 210, 574, 629
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 3 cut(s) 58, 181, 810
Van91I CCANNNNNTGG 1 cut(s) 224
VspI ATTAAT 2 cut(s) 117, 788
XapI RAATTY 2 cut(s) 591, 1484
XbaI TCTAGA 1 cut(s) 1151
XceI RCATGY 2 cut(s) 418, 736
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 3 cut(s) 317, 1152, 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.