Rmu_sc0001969.1_g000002

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001969.1
OG0000697
Physical Location & Seq
Reverse (-)
2178 .. 6548
4371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001969.1_g000002.1.cds

Sequence Viewer

Length: 756 bp
atgaagaactttggtgttgggagttttgtcaaattccgactttggtccggcagcggtgccgccactagggctgtcaatgggtcgtgtcggtcgtgttatcgggtcgtgtcggaattgacagccctagccgccactgtggtggtgttttccggcggaaaatattctccagtggattgtgaatttgctaatcgacgccaatctttagctccattaagcggtgtccaatatcatctgaaagattttggtggtcaaggtcgccaccccagaaatgcaagtgagttgttcaatcttcgccatgcatcattaaggaatgtggttgagaggacatttggtatctttaaatcgtggttcacaattttcaaaatcgcacctccattcccatttgagacacaagaggagttagtgttagcttgtgttggactacataactttcttcgcaaaaaatgtcgctccgatgaatttcccgttgaaccagaagatgatcagtcttcatcatatctagacatggaagatgaaaatattgaactactttctcaaagccaacaacaacaaagagcggaagctaatgcttggagaattagcattgctgatgctatgtggaatgacaggccgcagaatgatgataatggaaatcaagaggataacaaggaggatcaagacaatgacaatgagaataatgaggaacacataaatgatgagaatcaagaggtttacgatgataatgatgttggaatggaggagtatgcatcattctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

251

Amino Acids

28.55

Weight (kDa)

4.68

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 557
AciI CCGC 7 cut(s) 54, 60, 129, 153, 216, 557, 611
AclWI GGATC 1 cut(s) 660
AcsI RAATTY 3 cut(s) 32, 179, 458
AcyI GRCGYC 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 66, 215
AgsI TTSAA 4 cut(s) 286, 361, 470, 524
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 3 cut(s) 206, 410, 563
AluI AGCT 3 cut(s) 206, 410, 563
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 1 cut(s) 660
AoxI GGCC 1 cut(s) 608
ApeKI GCWGC 1 cut(s) 51
ApoI RAATTY 3 cut(s) 32, 179, 458
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 45
AvaII GGWCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 63
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 3 cut(s) 66, 125, 500
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 4 cut(s) 52, 60, 129, 611
BlsI GCNGC 4 cut(s) 53, 61, 130, 612
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 58
BmsI GCATC 2 cut(s) 308, 580
BoxI GACNNNNGTC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 215
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 699
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 2 cut(s) 66, 215
BseMI GCAATG 1 cut(s) 582
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 410, 752
BseXI GCAGC 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 610
BshNI GGYRCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 92
BsiSI CCGG 2 cut(s) 48, 150
BslI CCNNNNNNNGG 2 cut(s) 66, 215
BsmAI GTCTC 1 cut(s) 380
BsnI GGCC 1 cut(s) 610
Bsp143I GATC 2 cut(s) 481, 652
BspACI CCGC 7 cut(s) 54, 60, 129, 153, 216, 557, 611
BspANI GGCC 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 582
BsrI ACTGG 1 cut(s) 167
BssMI GATC 2 cut(s) 481, 652
BssNI GRCGYC 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 193
BstKTI GATC 2 cut(s) 484, 655
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 2 cut(s) 481, 652
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 68, 128
BstPAI GACNNNNGTC 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 480
BstXI CCANNNNNNTGG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 132, 174
Cfr13I GGNCC 1 cut(s) 45
CseI GACGC 1 cut(s) 201
CviAII CATG 2 cut(s) 296, 505
CviJI RGCY 8 cut(s) 71, 122, 128, 206, 410, 540, 563, 610
CviKI_1 RGCY 8 cut(s) 71, 122, 128, 206, 410, 540, 563, 610
DpnI GATC 2 cut(s) 483, 654
DpnII GATC 2 cut(s) 481, 652
DraI TTTAAA 1 cut(s) 340
EciI GGCGGA 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 45
EcoT22I ATGCAT 2 cut(s) 301, 748
FaeI CATG 2 cut(s) 299, 508
FaiI YATR 7 cut(s) 297, 426, 496, 506, 596, 689, 744
FatI CATG 2 cut(s) 295, 504
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 4 cut(s) 52, 60, 129, 611
Fsp4HI GCNGC 4 cut(s) 52, 60, 129, 611
FspBI CTAG 3 cut(s) 66, 125, 500
GluI GCNGC 4 cut(s) 52, 60, 129, 611
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 1 cut(s) 610
HapII CCGG 2 cut(s) 48, 150
HgaI GACGC 1 cut(s) 201
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 299, 508
HinfI GANTC 1 cut(s) 700
HpaII CCGG 2 cut(s) 48, 150
Hpy166II GTNNAC 2 cut(s) 351, 712
Hpy188I TCNGA 4 cut(s) 38, 112, 234, 454
Hpy188III TCNNGA 4 cut(s) 500, 635, 656, 704
Hpy8I GTNNAC 2 cut(s) 351, 712
Hpy99I CGWCG 1 cut(s) 195
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4V TGCA 3 cut(s) 272, 299, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 128
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 299, 508
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 2 cut(s) 481, 652
LmnI GCTCC 2 cut(s) 211, 455
LpnPI CCDG 6 cut(s) 61, 163, 180, 277, 486, 592
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 2 cut(s) 308, 580
MaeI CTAG 3 cut(s) 66, 125, 500
MalI GATC 2 cut(s) 483, 654
MbiI CCGCTC 1 cut(s) 557
MboI GATC 2 cut(s) 481, 652
MboII GAAGA 6 cut(s) 16, 281, 425, 480, 488, 521
MluCI AATT 6 cut(s) 32, 113, 179, 354, 458, 576
MmeI TCCRAC 4 cut(s) 61, 90, 397, 709
MnlI CCTC 8 cut(s) 315, 381, 388, 631, 643, 673, 700, 730
Mph1103I ATGCAT 2 cut(s) 301, 748
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 3 cut(s) 212, 305, 339
MslI CAYNNNNRTG 2 cut(s) 137, 690
MspA1I CMGCKG 1 cut(s) 54
MspI CCGG 2 cut(s) 48, 150
MwoI GCNNNNNNNGC 2 cut(s) 68, 128
NdeII GATC 2 cut(s) 481, 652
NlaIII CATG 2 cut(s) 299, 508
NlaIV GGNNCC 1 cut(s) 58
NsiI ATGCAT 2 cut(s) 301, 748
OliI CACNNNNGTG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 700
PkrI GCNGC 4 cut(s) 53, 61, 130, 612
PshAI GACNNNNGTC 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 45
RseI CAYNNNNRTG 2 cut(s) 137, 690
SaqAI TTAA 3 cut(s) 212, 305, 339
SatI GCNGC 4 cut(s) 52, 60, 129, 611
Sau3AI GATC 2 cut(s) 481, 652
Sau96I GGNCC 1 cut(s) 45
SetI ASST 6 cut(s) 208, 256, 373, 412, 565, 711
SfaNI GCATC 2 cut(s) 308, 580
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 137, 690
Sse9I AATT 6 cut(s) 32, 113, 179, 354, 458, 576
SsiI CCGC 7 cut(s) 54, 60, 129, 153, 216, 557, 611
SspI AATATT 2 cut(s) 161, 520
SspMI CTAG 3 cut(s) 66, 125, 500
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 190
TaqII GACCGA 1 cut(s) 78
TasI AATT 6 cut(s) 32, 113, 179, 354, 458, 576
TauI GCSGC 3 cut(s) 62, 131, 613
TfiI GAWTC 1 cut(s) 700
Tru1I TTAA 3 cut(s) 212, 305, 339
Tru9I TTAA 3 cut(s) 212, 305, 339
TscAI CASTG 2 cut(s) 139, 174
TseI GCWGC 1 cut(s) 51
TspDTI ATGAA 4 cut(s) 17, 471, 480, 528
TspRI CASTG 2 cut(s) 139, 174
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 3 cut(s) 32, 179, 458
XbaI TCTAGA 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 3 cut(s) 66, 125, 500
Zsp2I ATGCAT 2 cut(s) 301, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.