Rmu_sc0017974.1_g000001

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017974.1
OG0000697
Physical Location & Seq
Reverse (-)
3258 .. 4392
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017974.1_g000001.1.cds

Sequence Viewer

Length: 849 bp
atgattgtggggcataatgatcgatattgtaatgttcgtcataggtttggtcgctcacatttcgcaactagtcaaaacttcaacaaagttttaaaggccttgaatactatagcaccacaaatgatggtcaaacctggaggtgtgccctctaaaattagggaaagtacaaggttttacccttattttaaggattgcattggtgctattgatggaacccacattccagccatggtaaaaggtcgagaagtaagcagctatcgtaaccgtcatggtattcaatctcaaaatgttttggctgcttgcaatttcgatttggaattcatatacgtgcttagtgggtgggaaggaaaatattttctcgtggattgtggatttgctaatcgacgccaatttttagctccactacgaggtgtccgatatcatctgaaagattttggtggtcaaggtcgccaccccagaaatgcaagtgagttgtttaatcttcaccatgcatcattgaggaatgtgattgaaaggatatttggtatctttaaatcacggttcacaattttcaaaatctcacctcccttcccatttgagacacaagcggagttagtgttagcttgtgctggactacataactttcttcttaaagaatgtcgctccgatgaatttcccgttgaaccagaagatgatcaatcttcatcatatctagacatggaagatgaaaatcttgaactactttctcaaagccaacaacaacaaagagtggaagctaatgcttggagaattagcattgctgatgctatgtggaatgataggccgcggaatgatgataatgcaaatcaagaggataacaatgaggattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

282

Amino Acids

32.48

Weight (kDa)

6.8

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 805
AciI CCGC 3 cut(s) 587, 803, 805
AcsI RAATTY 2 cut(s) 317, 650
AcyI GRCGYC 1 cut(s) 385
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 7 cut(s) 82, 103, 278, 512, 553, 662, 716
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 1 cut(s) 133
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AloI GAACNNNNNNTCC 2 cut(s) 205, 237
AluBI AGCT 4 cut(s) 255, 398, 602, 755
AluI AGCT 4 cut(s) 255, 398, 602, 755
Alw26I GTCTC 1 cut(s) 572
AoxI GGCC 2 cut(s) 96, 800
ApeKI GCWGC 2 cut(s) 252, 296
ApoI RAATTY 2 cut(s) 317, 650
AsuHPI GGTGA 2 cut(s) 476, 552
BaeGI GKGCMC 1 cut(s) 147
BauI CACGAG 1 cut(s) 359
BbvI GCAGC 2 cut(s) 264, 283
BccI CCATC 2 cut(s) 118, 203
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 1 cut(s) 572
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 2 cut(s) 69, 692
BfmI CTRYAG 1 cut(s) 108
BisI GCNGC 3 cut(s) 253, 297, 803
BlsI GCNGC 3 cut(s) 254, 298, 804
Bme1390I CCNGG 1 cut(s) 135
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 500, 772
BpmI CTGGAG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 328
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 385
BsaJI CCNNGG 2 cut(s) 228, 803
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 2 cut(s) 228, 803
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMI GCAATG 1 cut(s) 774
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 2 cut(s) 264, 283
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 2 cut(s) 98, 802
BshVI ATCGAT 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 572
BsnI GGCC 2 cut(s) 98, 802
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 19, 673
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 3 cut(s) 587, 803, 805
BspANI GGCC 2 cut(s) 98, 802
BspDI ATCGAT 1 cut(s) 22
BspFNI CGCG 1 cut(s) 805
BspLI GGNNCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 774
BssECI CCNNGG 2 cut(s) 228, 803
BssMI GATC 2 cut(s) 19, 673
BssNI GRCGYC 1 cut(s) 385
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 266, 540
BstACI GRCGYC 1 cut(s) 385
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 332
BstDSI CCRYGG 2 cut(s) 228, 803
BstFNI CGCG 1 cut(s) 805
BstKTI GATC 2 cut(s) 22, 676
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 2 cut(s) 19, 673
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 2 cut(s) 264, 283
Bsu15I ATCGAT 1 cut(s) 22
BsuRI GGCC 2 cut(s) 98, 802
BsuTUI ATCGAT 1 cut(s) 22
BtgI CCRYGG 2 cut(s) 228, 803
Cac8I GCNNGC 1 cut(s) 301
Cfr42I CCGCGG 1 cut(s) 806
ClaI ATCGAT 1 cut(s) 22
CseI GACGC 1 cut(s) 393
Csp6I GTAC 1 cut(s) 165
CviAII CATG 4 cut(s) 229, 269, 488, 697
CviJI RGCY 9 cut(s) 98, 227, 255, 296, 398, 602, 732, 755, 802
CviKI_1 RGCY 9 cut(s) 98, 227, 255, 296, 398, 602, 732, 755, 802
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 1 cut(s) 332
DpnI GATC 2 cut(s) 21, 675
DpnII GATC 2 cut(s) 19, 673
DraI TTTAAA 2 cut(s) 93, 532
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 98
Eco32I GATATC 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 133
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 4 cut(s) 232, 272, 491, 700
FatI CATG 4 cut(s) 228, 268, 487, 696
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 3 cut(s) 253, 297, 803
Fsp4HI GCNGC 3 cut(s) 253, 297, 803
FspBI CTAG 2 cut(s) 69, 692
GluI GCNGC 3 cut(s) 253, 297, 803
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 2 cut(s) 98, 802
HgaI GACGC 1 cut(s) 393
Hin1I GRCGYC 1 cut(s) 385
Hin1II CATG 4 cut(s) 232, 272, 491, 700
HphI GGTGA 2 cut(s) 476, 552
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 3 cut(s) 416, 426, 646
Hpy188III TCNNGA 4 cut(s) 242, 692, 713, 827
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 338, 577
HpyCH4III ACNGT 2 cut(s) 266, 540
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 5 cut(s) 195, 303, 464, 491, 821
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 385
Hsp92II CATG 4 cut(s) 232, 272, 491, 700
Ksp22I TGATCA 1 cut(s) 673
KspI CCGCGG 1 cut(s) 806
Kzo9I GATC 2 cut(s) 19, 673
LmnI GCTCC 2 cut(s) 403, 647
LpnPI CCDG 6 cut(s) 120, 147, 237, 469, 594, 678
Lsp1109I GCAGC 2 cut(s) 264, 283
LweI GCATC 2 cut(s) 500, 772
MaeI CTAG 2 cut(s) 69, 692
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 260
MalI GATC 2 cut(s) 21, 675
MboI GATC 2 cut(s) 19, 673
MboII GAAGA 5 cut(s) 473, 617, 672, 680, 713
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 7 cut(s) 153, 304, 317, 389, 546, 650, 768
MnlI CCTC 7 cut(s) 131, 157, 401, 492, 573, 823, 835
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 6 cut(s) 92, 186, 477, 531, 630, 847
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 805
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 805
NcoI CCATGG 1 cut(s) 228
NdeII GATC 2 cut(s) 19, 673
NlaIII CATG 4 cut(s) 232, 272, 491, 700
NlaIV GGNNCC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 493
PceI AGGCCT 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PkrI GCNGC 3 cut(s) 254, 298, 804
Ppu21I YACGTR 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 214
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 326
SacII CCGCGG 1 cut(s) 806
SaqAI TTAA 6 cut(s) 92, 186, 477, 531, 630, 847
SatI GCNGC 3 cut(s) 253, 297, 803
Sau3AI GATC 2 cut(s) 19, 673
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 2 cut(s) 500, 772
SfcI CTRYAG 1 cut(s) 108
Sfr303I CCGCGG 1 cut(s) 806
SgrBI CCGCGG 1 cut(s) 806
SmiMI CAYNNNNRTG 1 cut(s) 326
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 7 cut(s) 153, 304, 317, 389, 546, 650, 768
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 3 cut(s) 587, 803, 805
SspI AATATT 1 cut(s) 353
SspMI CTAG 2 cut(s) 69, 692
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 2 cut(s) 266, 540
TaiI ACGT 1 cut(s) 330
TaqI TCGA 4 cut(s) 22, 241, 309, 382
TasI AATT 7 cut(s) 153, 304, 317, 389, 546, 650, 768
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 805
Tru1I TTAA 6 cut(s) 92, 186, 477, 531, 630, 847
Tru9I TTAA 6 cut(s) 92, 186, 477, 531, 630, 847
TseI GCWGC 2 cut(s) 252, 296
TspDTI ATGAA 4 cut(s) 310, 663, 672, 720
XapI RAATTY 2 cut(s) 317, 650
XbaI TCTAGA 1 cut(s) 691
XspI CTAG 2 cut(s) 69, 692
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.