Rroxscaffold_4G00318480

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000697
Physical Location & Seq
Reverse (-)
44643679 .. 44652757
9079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318480.1

Sequence Viewer

Length: 312 bp
ATGCTTGCAACGTTTTCACTAATTGTCGGCCAAAACAATCGATACGGTGAACCTCAGCTGATATTTGAGCGATCTCATTTCACTGTAAGCAAAGTCATGAAGGCTTTGAATATAATAGCACCAAATTTTATGGCTAAACCTGAGTCCATACCACCTAACATAAGAGAAAATACAAGGTTTTATCCTTACTTGAAGAAGATGCAGCGGGAATTAGAGACAAAAGTACTCTTTCTTTTCTTTGCTCTCCACTTCGGCCAGCTACTCATTCTCTCTGCACATGCTCCGGACAACACTTTCCTCATTTTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

103

Amino Acids

11.99

Weight (kDa)

9.69

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 1 cut(s) 205
AclI AACGTT 1 cut(s) 11
AcoI YGGCCR 2 cut(s) 28, 253
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 1 cut(s) 225
AgsI TTSAA 2 cut(s) 109, 193
AluBI AGCT 2 cut(s) 58, 259
AluI AGCT 2 cut(s) 58, 259
Alw26I GTCTC 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 2 cut(s) 28, 253
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 59
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 209
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
BmcAI AGTACT 1 cut(s) 225
BmsI GCATC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 54
Bsa29I ATCGAT 1 cut(s) 40
BsaWI WCCGGW 1 cut(s) 283
BseAI TCCGGA 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 40
BseMII CTCAG 2 cut(s) 68, 132
BseXI GCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 2 cut(s) 30, 255
BshVI ATCGAT 1 cut(s) 40
BsiSI CCGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 209
BsnI GGCC 2 cut(s) 30, 255
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 1 cut(s) 71
BspACI CCGC 1 cut(s) 205
BspANI GGCC 2 cut(s) 30, 255
BspCNI CTCAG 2 cut(s) 67, 133
BspDI ATCGAT 1 cut(s) 40
BspEI TCCGGA 1 cut(s) 283
BspHI TCATGA 1 cut(s) 96
BssMI GATC 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 47, 85
BstC8I GCNNGC 2 cut(s) 6, 257
BstDEI CTNAG 2 cut(s) 54, 141
BstKTI GATC 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 40
BsuRI GGCC 2 cut(s) 30, 255
BsuTUI ATCGAT 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 6, 257
CciI TCATGA 1 cut(s) 96
ClaI ATCGAT 1 cut(s) 40
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 97, 278
CviJI RGCY 6 cut(s) 30, 58, 104, 134, 255, 259
CviKI_1 RGCY 6 cut(s) 30, 58, 104, 134, 255, 259
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 2 cut(s) 54, 141
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 28, 253
FaeI CATG 2 cut(s) 100, 281
FaiI YATR 6 cut(s) 98, 113, 131, 149, 161, 279
FatI CATG 2 cut(s) 96, 277
FauI CCCGC 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 203
GluI GCNGC 1 cut(s) 203
HaeIII GGCC 2 cut(s) 30, 255
HapII CCGG 1 cut(s) 284
Hin1II CATG 2 cut(s) 100, 281
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 284
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 50
Hpy188III TCNNGA 2 cut(s) 97, 284
Hpy8I GTNNAC 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 47, 85
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 8, 202, 275
HpyF3I CTNAG 2 cut(s) 54, 141
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 2 cut(s) 100, 281
Kpn2I TCCGGA 1 cut(s) 283
Kzo9I GATC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 3 cut(s) 153, 269, 297
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 189
MaeII ACGT 1 cut(s) 11
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 2 cut(s) 205, 208
MluCI AATT 3 cut(s) 21, 124, 209
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 2 cut(s) 63, 308
MroI TCCGGA 1 cut(s) 283
MspA1I CMGCKG 2 cut(s) 58, 205
MspI CCGG 1 cut(s) 284
NdeII GATC 1 cut(s) 71
NlaIII CATG 2 cut(s) 100, 281
NspI RCATGY 1 cut(s) 281
PagI TCATGA 1 cut(s) 96
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp1406I AACGTT 1 cut(s) 11
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 1 cut(s) 71
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 152
SetI ASST 7 cut(s) 14, 55, 60, 142, 157, 179, 261
SfaNI GCATC 1 cut(s) 189
SgeI CNNG 9 cut(s) 17, 109, 152, 186, 202, 218, 268, 290, 296
Sse9I AATT 3 cut(s) 21, 124, 209
SsiI CCGC 1 cut(s) 205
TaaI ACNGT 2 cut(s) 47, 85
TaiI ACGT 1 cut(s) 14
TaqI TCGA 1 cut(s) 40
TasI AATT 3 cut(s) 21, 124, 209
TatI WGTACW 1 cut(s) 223
TscAI CASTG 1 cut(s) 88
TseI GCWGC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 1 cut(s) 88
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.