nuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 389 |
| AccII | CGCG | 1 cut(s) | 643 |
| AciI | CCGC | 2 cut(s) | 387, 474 |
| AclWI | GGATC | 1 cut(s) | 773 |
| AcoI | YGGCCR | 1 cut(s) | 46 |
| AcsI | RAATTY | 1 cut(s) | 755 |
| AcyI | GRCGYC | 1 cut(s) | 490 |
| AfaI | GTAC | 3 cut(s) | 202, 366, 517 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 512 |
| AjiI | CACGTC | 1 cut(s) | 278 |
| AjnI | CCWGG | 1 cut(s) | 448 |
| AluBI | AGCT | 5 cut(s) | 71, 239, 335, 503, 697 |
| AluI | AGCT | 5 cut(s) | 71, 239, 335, 503, 697 |
| AlwI | GGATC | 1 cut(s) | 773 |
| AoxI | GGCC | 2 cut(s) | 46, 132 |
| ApeKI | GCWGC | 1 cut(s) | 332 |
| ApoI | RAATTY | 1 cut(s) | 755 |
| AsuHPI | GGTGA | 1 cut(s) | 557 |
| BbvI | GCAGC | 1 cut(s) | 344 |
| BccI | CCATC | 3 cut(s) | 239, 369, 564 |
| BceAI | ACGGC | 1 cut(s) | 729 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 723, 757 |
| BciT130I | CCWGG | 1 cut(s) | 450 |
| BciVI | GTATCC | 1 cut(s) | 33 |
| BfaI | CTAG | 2 cut(s) | 423, 698 |
| BfmI | CTRYAG | 1 cut(s) | 240 |
| BfuI | GTATCC | 1 cut(s) | 33 |
| BisI | GCNGC | 1 cut(s) | 333 |
| BlsI | GCNGC | 1 cut(s) | 334 |
| Bme1390I | CCNGG | 1 cut(s) | 450 |
| BmgBI | CACGTC | 1 cut(s) | 278 |
| BmiI | GGNNCC | 1 cut(s) | 385 |
| BmrFI | CCNGG | 1 cut(s) | 450 |
| BmsI | GCATC | 2 cut(s) | 403, 883 |
| BpuEI | CTTGAG | 1 cut(s) | 622 |
| Bsa29I | ATCGAT | 1 cut(s) | 58 |
| BsaBI | GATNNNNATC | 1 cut(s) | 21 |
| BsaHI | GRCGYC | 1 cut(s) | 490 |
| BsaJI | CCNNGG | 1 cut(s) | 304 |
| BsaWI | WCCGGW | 1 cut(s) | 151 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 853, 883 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 512 |
| Bse118I | RCCGGY | 1 cut(s) | 492 |
| Bse8I | GATNNNNATC | 1 cut(s) | 21 |
| BseBI | CCWGG | 1 cut(s) | 450 |
| BseCI | ATCGAT | 1 cut(s) | 58 |
| BseDI | CCNNGG | 1 cut(s) | 304 |
| BseGI | GGATG | 3 cut(s) | 380, 556, 820 |
| BseJI | GATNNNNATC | 1 cut(s) | 21 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 512 |
| BseMII | CTCAG | 1 cut(s) | 162 |
| BseXI | GCAGC | 1 cut(s) | 344 |
| BsgI | GTGCAG | 2 cut(s) | 704, 732 |
| Bsh1236I | CGCG | 1 cut(s) | 643 |
| BshFI | GGCC | 2 cut(s) | 48, 134 |
| BshVI | ATCGAT | 1 cut(s) | 58 |
| BsiSI | CCGG | 2 cut(s) | 152, 493 |
| BslFI | GGGAC | 1 cut(s) | 482 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 512 |
| BsmFI | GGGAC | 1 cut(s) | 482 |
| BsmI | GAATGC | 1 cut(s) | 746 |
| BsnI | GGCC | 2 cut(s) | 48, 134 |
| Bsp143I | GATC | 3 cut(s) | 89, 778, 832 |
| Bsp19I | CCATGG | 1 cut(s) | 304 |
| BspACI | CCGC | 2 cut(s) | 387, 474 |
| BspANI | GGCC | 2 cut(s) | 48, 134 |
| BspCNI | CTCAG | 1 cut(s) | 163 |
| BspDI | ATCGAT | 1 cut(s) | 58 |
| BspFNI | CGCG | 1 cut(s) | 643 |
| BspHI | TCATGA | 1 cut(s) | 391 |
| BspLI | GGNNCC | 1 cut(s) | 385 |
| BspPI | GGATC | 1 cut(s) | 773 |
| BsrBI | CCGCTC | 1 cut(s) | 389 |
| BsrFI | RCCGGY | 1 cut(s) | 492 |
| BssAI | RCCGGY | 1 cut(s) | 492 |
| BssECI | CCNNGG | 1 cut(s) | 304 |
| BssMI | GATC | 3 cut(s) | 89, 778, 832 |
| BssNI | GRCGYC | 1 cut(s) | 490 |
| BssT1I | CCWWGG | 1 cut(s) | 304 |
| Bst2UI | CCWGG | 1 cut(s) | 450 |
| Bst4CI | ACNGT | 4 cut(s) | 65, 103, 268, 354 |
| BstACI | GRCGYC | 1 cut(s) | 490 |
| BstDEI | CTNAG | 3 cut(s) | 171, 368, 463 |
| BstDSI | CCRYGG | 1 cut(s) | 304 |
| BstF5I | GGATG | 3 cut(s) | 380, 556, 820 |
| BstFNI | CGCG | 1 cut(s) | 643 |
| BstKTI | GATC | 3 cut(s) | 92, 781, 835 |
| BstMBI | GATC | 3 cut(s) | 89, 778, 832 |
| BstNI | CCWGG | 1 cut(s) | 450 |
| BstSCI | CCNGG | 1 cut(s) | 448 |
| BstSFI | CTRYAG | 1 cut(s) | 240 |
| BstUI | CGCG | 1 cut(s) | 643 |
| BstV1I | GCAGC | 1 cut(s) | 344 |
| BstX2I | RGATCY | 1 cut(s) | 778 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 905 |
| BstYI | RGATCY | 1 cut(s) | 778 |
| Bsu15I | ATCGAT | 1 cut(s) | 58 |
| BsuI | GTATCC | 1 cut(s) | 33 |
| BsuRI | GGCC | 2 cut(s) | 48, 134 |
| BsuTUI | ATCGAT | 1 cut(s) | 58 |
| BtgI | CCRYGG | 1 cut(s) | 304 |
| BtrI | CACGTC | 1 cut(s) | 278 |
| BtsCI | GGATG | 3 cut(s) | 380, 556, 820 |
| BtsIMutI | CAGTG | 2 cut(s) | 70, 99 |
| CciI | TCATGA | 1 cut(s) | 391 |
| Cfr10I | RCCGGY | 1 cut(s) | 492 |
| ClaI | ATCGAT | 1 cut(s) | 58 |
| CseI | GACGC | 2 cut(s) | 220, 498 |
| CsiI | ACCWGGT | 1 cut(s) | 448 |
| Csp6I | GTAC | 3 cut(s) | 201, 365, 516 |
| CviAII | CATG | 3 cut(s) | 305, 392, 593 |
| CviQI | GTAC | 3 cut(s) | 201, 365, 516 |
| DdeI | CTNAG | 3 cut(s) | 171, 368, 463 |
| DpnI | GATC | 3 cut(s) | 91, 780, 834 |
| DpnII | GATC | 3 cut(s) | 89, 778, 832 |
| EaeI | YGGCCR | 1 cut(s) | 46 |
| Eco130I | CCWWGG | 1 cut(s) | 304 |
| Eco147I | AGGCCT | 1 cut(s) | 134 |
| Eco32I | GATATC | 3 cut(s) | 293, 315, 524 |
| EcoRII | CCWGG | 1 cut(s) | 448 |
| EcoRV | GATATC | 3 cut(s) | 293, 315, 524 |
| EcoT14I | CCWWGG | 1 cut(s) | 304 |
| ErhI | CCWWGG | 1 cut(s) | 304 |
| FaeI | CATG | 3 cut(s) | 308, 395, 596 |
| FaqI | GGGAC | 1 cut(s) | 482 |
| FatI | CATG | 3 cut(s) | 304, 391, 592 |
| FauNDI | CATATG | 1 cut(s) | 882 |
| Fnu4HI | GCNGC | 1 cut(s) | 333 |
| FokI | GGATG | 3 cut(s) | 387, 543, 827 |
| Fsp4HI | GCNGC | 1 cut(s) | 333 |
| FspBI | CTAG | 2 cut(s) | 423, 698 |
| GluI | GCNGC | 1 cut(s) | 333 |
| HaeIII | GGCC | 2 cut(s) | 48, 134 |
| HapII | CCGG | 2 cut(s) | 152, 493 |
| HgaI | GACGC | 2 cut(s) | 220, 498 |
| Hin1I | GRCGYC | 1 cut(s) | 490 |
| Hin1II | CATG | 3 cut(s) | 308, 395, 596 |
| HinfI | GANTC | 3 cut(s) | 173, 395, 435 |
| HpaII | CCGG | 2 cut(s) | 152, 493 |
| HphI | GGTGA | 1 cut(s) | 557 |
| Hpy166II | GTNNAC | 1 cut(s) | 888 |
| Hpy188I | TCNGA | 3 cut(s) | 236, 751, 783 |
| Hpy188III | TCNNGA | 5 cut(s) | 127, 392, 423, 764, 794 |
| Hpy8I | GTNNAC | 1 cut(s) | 888 |
| Hpy99I | CGWCG | 2 cut(s) | 236, 492 |
| HpyAV | CCTTC | 3 cut(s) | 124, 542, 941 |
| HpyCH4III | ACNGT | 4 cut(s) | 65, 103, 268, 354 |
| HpyCH4IV | ACGT | 1 cut(s) | 277 |
| HpyCH4V | TGCA | 3 cut(s) | 569, 713, 721 |
| HpyF3I | CTNAG | 3 cut(s) | 171, 368, 463 |
| HpySE526I | ACGT | 1 cut(s) | 277 |
| Hsp92I | GRCGYC | 1 cut(s) | 490 |
| Hsp92II | CATG | 3 cut(s) | 308, 395, 596 |
| Kzo9I | GATC | 3 cut(s) | 89, 778, 832 |
| LmnI | GCTCC | 2 cut(s) | 236, 508 |
| Lsp1109I | GCAGC | 1 cut(s) | 344 |
| LweI | GCATC | 2 cut(s) | 403, 883 |
| MabI | ACCWGGT | 1 cut(s) | 448 |
| MaeI | CTAG | 2 cut(s) | 423, 698 |
| MaeII | ACGT | 1 cut(s) | 277 |
| MaeIII | GTNAC | 1 cut(s) | 338 |
| MalI | GATC | 3 cut(s) | 91, 780, 834 |
| MbiI | CCGCTC | 1 cut(s) | 389 |
| MboI | GATC | 3 cut(s) | 89, 778, 832 |
| MboII | GAAGA | 8 cut(s) | 322, 578, 646, 722, 785, 788, 809, 944 |
| MfeI | CAATTG | 1 cut(s) | 570 |
| MflI | RGATCY | 1 cut(s) | 778 |
| MluCI | AATT | 7 cut(s) | 12, 454, 570, 612, 724, 755, 802 |
| MlyI | GAGTC | 2 cut(s) | 182, 429 |
| MmeI | TCCRAC | 3 cut(s) | 50, 214, 253 |
| MnlI | CCTC | 5 cut(s) | 274, 373, 506, 597, 771 |
| MseI | TTAA | 2 cut(s) | 222, 636 |
| MspI | CCGG | 2 cut(s) | 152, 493 |
| MspR9I | CCNGG | 1 cut(s) | 450 |
| MunI | CAATTG | 1 cut(s) | 570 |
| Mva1269I | GAATGC | 1 cut(s) | 746 |
| MvaI | CCWGG | 1 cut(s) | 450 |
| MvnI | CGCG | 1 cut(s) | 643 |
| NcoI | CCATGG | 1 cut(s) | 304 |
| NdeI | CATATG | 1 cut(s) | 882 |
| NdeII | GATC | 3 cut(s) | 89, 778, 832 |
| NlaIII | CATG | 3 cut(s) | 308, 395, 596 |
| NlaIV | GGNNCC | 1 cut(s) | 385 |
| NmeAIII | GCCGAG | 1 cut(s) | 52 |
| PagI | TCATGA | 1 cut(s) | 391 |
| PceI | AGGCCT | 1 cut(s) | 134 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 517 |
| PctI | GAATGC | 1 cut(s) | 746 |
| PfeI | GAWTC | 1 cut(s) | 395 |
| PkrI | GCNGC | 1 cut(s) | 334 |
| PleI | GAGTC | 2 cut(s) | 181, 429 |
| PpsI | GAGTC | 2 cut(s) | 181, 429 |
| Psp6I | CCWGG | 1 cut(s) | 448 |
| PspGI | CCWGG | 1 cut(s) | 448 |
| PspN4I | GGNNCC | 1 cut(s) | 385 |
| PsuI | RGATCY | 1 cut(s) | 778 |
| RsaI | GTAC | 3 cut(s) | 202, 366, 517 |
| RsaNI | GTAC | 3 cut(s) | 201, 365, 516 |
| SaqAI | TTAA | 2 cut(s) | 222, 636 |
| SatI | GCNGC | 1 cut(s) | 333 |
| Sau3AI | GATC | 3 cut(s) | 89, 778, 832 |
| SchI | GAGTC | 2 cut(s) | 182, 429 |
| ScrFI | CCNGG | 1 cut(s) | 450 |
| SexAI | ACCWGGT | 1 cut(s) | 448 |
| SfaNI | GCATC | 2 cut(s) | 403, 883 |
| SfcI | CTRYAG | 1 cut(s) | 240 |
| SmlI | CTYRAG | 1 cut(s) | 601 |
| SmoI | CTYRAG | 1 cut(s) | 601 |
| Sse9I | AATT | 7 cut(s) | 12, 454, 570, 612, 724, 755, 802 |
| SseBI | AGGCCT | 1 cut(s) | 134 |
| SsiI | CCGC | 2 cut(s) | 387, 474 |
| SspI | AATATT | 2 cut(s) | 624, 633 |
| SspMI | CTAG | 2 cut(s) | 423, 698 |
| StuI | AGGCCT | 1 cut(s) | 134 |
| StyD4I | CCNGG | 1 cut(s) | 448 |
| StyI | CCWWGG | 1 cut(s) | 304 |
| TaaI | ACNGT | 4 cut(s) | 65, 103, 268, 354 |
| TaiI | ACGT | 1 cut(s) | 280 |
| TaqI | TCGA | 6 cut(s) | 58, 295, 487, 511, 520, 733 |
| TasI | AATT | 7 cut(s) | 12, 454, 570, 612, 724, 755, 802 |
| TatI | WGTACW | 2 cut(s) | 200, 364 |
| TfiI | GAWTC | 1 cut(s) | 395 |
| Tru1I | TTAA | 2 cut(s) | 222, 636 |
| Tru9I | TTAA | 2 cut(s) | 222, 636 |
| TscAI | CASTG | 2 cut(s) | 70, 106 |
| TseI | GCWGC | 1 cut(s) | 332 |
| TspDTI | ATGAA | 7 cut(s) | 346, 666, 768, 870, 930, 945, 960 |
| TspGWI | ACGGA | 1 cut(s) | 166 |
| TspRI | CASTG | 2 cut(s) | 70, 106 |
| XapI | RAATTY | 1 cut(s) | 755 |
| XbaI | TCTAGA | 1 cut(s) | 422 |
| XspI | CTAG | 2 cut(s) | 423, 698 |