Rmu_sc0003113.1_g000003

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003113.1
OG0000697
Physical Location & Seq
Reverse (-)
7205 .. 8698
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003113.1_g000003.1.cds

Sequence Viewer

Length: 1014 bp
atggaaggggaagaaatagatggggaagagataggggaagaagaaatagatgaagacgaaattgaagaggaattttacgaaacaatcaagtatttattgatggcatttcaggcaataatcattgtgttaagagagactatgagtatgatgcatcaacgtattcaccgtcctctcaaacgacgacctattactaagaaaggatatcattacatacatggtttcttgaaagaagacccacaaagttttcgacaattgcacagaatgtatccaaatgtgtttctaaagttgtgtacggccacaatgatcgatatggtaatgttcgggaaaggtttggtcatttcacattttgctactagtcaaaacttcaacaaaactttgaaggccttgaacactatagcaccacgtatgatgatcaaacctggatctgcagtgccctctaaaataagggaaagcacaaggttttacccctactttaaggattgtattggtgcaatcgatggcgcccatattctagcgatggtaaaaggtcgggaagtaagcagctatcataatcgtcatggaattcagtctcaaaatgttttggcagctttggattgtggatttgctaatcgacgccaattcttagctccgttacgaggtgttcgatatcatctcaaagattttggtggtcaaggtcgccatcctcgaaatgcaagcgagttgttcaatcttcaccatgcgtcattgaggaatgtgattgagaggatatttggtatctttaaatcacggttcacaatttttaaaaccgcacctccatttccatatcaaacacaagcagaattggtgttagcttgtgctggactacacaactttcttcgcaaagaatgtcgtgaggatgaatttcctgttgaaccagaaaatgagtcatcatcttcatcctatctagatatggaagatgagaatcttgaactactttctcaaagtcaacaacgacaaagagcggaggctaatgcttggaggcttactattgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

337

Amino Acids

38.98

Weight (kDa)

7.25

Isoelectric Point (pI)

59.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35695
fragaria_vesca FvH4_4g17331 FvH4_6g20420 FvH4_6g24701 FvH4_6g52063
malus_domestica MD12G1024500.v1.1
prunus_persica Prupe.2G004900_v2.0.a1
pyrus_communis pycom10g09210 pycom111g03680 pycom12g13430 pycom16g19230
rosa_chinensis RchiOBHm_Chr5g0050651 RchiOBHm_Chr5g0054101 RchiOBHm_Chr6g0281391
rosa_multiflora Rmu_sc0000029.1_g000014 Rmu_sc0000147.1_g000039 Rmu_sc0000548.1_g000007 Rmu_sc0000913.1_g000001 Rmu_sc0000932.1_g000010 Rmu_sc0001304.1_g000041 Rmu_sc0001969.1_g000002 Rmu_sc0002357.1_g000042 Rmu_sc0002848.1_g000001 Rmu_sc0003113.1_g000003 Rmu_sc0003553.1_g000010 Rmu_sc0003642.1_g000003 Rmu_sc0004160.1_g000001 Rmu_sc0004511.1_g000001 Rmu_sc0004816.1_g000009 Rmu_sc0005500.1_g000008 Rmu_sc0005782.1_g000004 Rmu_sc0006833.1_g000005 Rmu_sc0007173.1_g000003 Rmu_sc0009973.1_g000001 Rmu_sc0016181.1_g000003 Rmu_sc0017974.1_g000001 Rmu_ssc0000255.1_g000020 Rmu_ssc0000263.1_g000011 Rmu_ssc0000366.1_g000012
rosa_roxburghii Rroxscaffold_1G00010110 Rroxscaffold_1G00023070 Rroxscaffold_1G00042980 Rroxscaffold_3G00273770 Rroxscaffold_4G00318480 Rroxscaffold_4G00323200 Rroxscaffold_5G00335150 Rroxscaffold_5G00340530 Rroxscaffold_5G00349500 Rroxscaffold_6G00401310 Rroxscaffold_6G00405610 Rroxscaffold_6G00409810 Rroxscaffold_7G00190250
rosa_rugosa Rorug04G0114300 Rorug05G0441600 Rorug07G0169600
rosa_wichuraiana Rw0G006340 Rw0G016280 Rw0G021420 Rw1G001890 Rw1G009310 Rw1G011130 Rw1G012600 Rw1G017460 Rw2G022560 Rw3G020960 Rw4G006850 Rw4G009950 Rw4G032810 Rw6G003640 Rw6G018030 Rw6G032240 Rw7G024530 Rw7G036060 Rw7G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 980
AciI CCGC 2 cut(s) 786, 980
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 3 cut(s) 71, 561, 878
AcyI GRCGYC 2 cut(s) 501, 613
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 635
AgsI TTSAA 8 cut(s) 65, 226, 367, 379, 388, 706, 890, 947
AhlI ACTAGT 1 cut(s) 353
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 4 cut(s) 543, 587, 626, 830
AluI AGCT 4 cut(s) 543, 587, 626, 830
Alw26I GTCTC 2 cut(s) 128, 573
AlwI GGATC 1 cut(s) 430
AoxI GGCC 2 cut(s) 294, 381
ApeKI GCWGC 2 cut(s) 540, 584
ApoI RAATTY 3 cut(s) 71, 561, 878
AspLEI GCGC 1 cut(s) 503
AsuHPI GGTGA 2 cut(s) 155, 704
BaeGI GKGCMC 1 cut(s) 435
BanI GGYRCC 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 60, 237
BbvI GCAGC 2 cut(s) 552, 596
BccI CCATC 5 cut(s) 14, 94, 491, 511, 687
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 276
BclI TGATCA 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 128, 573
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 3 cut(s) 354, 512, 923
BfmI CTRYAG 2 cut(s) 393, 426
BfoI RGCGCY 1 cut(s) 504
BfuI GTATCC 1 cut(s) 276
BisI GCNGC 2 cut(s) 541, 585
BlsI GCNGC 2 cut(s) 542, 586
Bme1390I CCNGG 1 cut(s) 420
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 138, 160
BpiI GAAGAC 2 cut(s) 60, 237
Bsa29I ATCGAT 2 cut(s) 306, 495
BsaAI YACGTR 1 cut(s) 404
BsaBI GATNNNNATC 1 cut(s) 939
BsaHI GRCGYC 2 cut(s) 501, 613
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 1 cut(s) 420
BseCI ATCGAT 2 cut(s) 306, 495
BseGI GGATG 3 cut(s) 679, 880, 914
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 2 cut(s) 552, 596
BshFI GGCC 2 cut(s) 296, 383
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 2 cut(s) 306, 495
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 2 cut(s) 128, 573
BsnI GGCC 2 cut(s) 296, 383
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 3 cut(s) 303, 411, 422
BspACI CCGC 2 cut(s) 786, 980
BspANI GGCC 2 cut(s) 296, 383
BspDI ATCGAT 2 cut(s) 306, 495
BspLI GGNNCC 1 cut(s) 502
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 980
BssMI GATC 3 cut(s) 303, 411, 422
BssNI GRCGYC 2 cut(s) 501, 613
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 167, 768
Bst6I CTCTTC 2 cut(s) 21, 60
BstACI GRCGYC 2 cut(s) 501, 613
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 694
BstDEI CTNAG 2 cut(s) 192, 622
BstF5I GGATG 3 cut(s) 679, 880, 914
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 3 cut(s) 306, 414, 425
BstMAI GTCTC 2 cut(s) 128, 573
BstMBI GATC 3 cut(s) 303, 411, 422
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 2 cut(s) 393, 426
BstSLI GKGCMC 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 552, 596
BstV2I GAAGAC 2 cut(s) 60, 237
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 2 cut(s) 306, 495
BsuI GTATCC 1 cut(s) 276
BsuRI GGCC 2 cut(s) 296, 383
BsuTUI ATCGAT 2 cut(s) 306, 495
BtgZI GCGATG 1 cut(s) 530
BtsCI GGATG 3 cut(s) 679, 880, 914
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 694
CfoI GCGC 1 cut(s) 503
ClaI ATCGAT 2 cut(s) 306, 495
CseI GACGC 2 cut(s) 621, 708
Csp6I GTAC 1 cut(s) 291
CviAII CATG 3 cut(s) 215, 557, 716
CviJI RGCY 8 cut(s) 296, 383, 543, 587, 626, 830, 986, 1000
CviKI_1 RGCY 8 cut(s) 296, 383, 543, 587, 626, 830, 986, 1000
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 2 cut(s) 192, 622
DinI GGCGCC 1 cut(s) 502
DpnI GATC 3 cut(s) 305, 413, 424
DpnII GATC 3 cut(s) 303, 411, 422
DraI TTTAAA 2 cut(s) 760, 781
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 21, 60
EarI CTCTTC 2 cut(s) 21, 60
Eco147I AGGCCT 1 cut(s) 383
Eco32I GATATC 2 cut(s) 203, 647
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 418
EcoRV GATATC 2 cut(s) 203, 647
EcoT22I ATGCAT 1 cut(s) 153
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
FaeI CATG 3 cut(s) 218, 560, 719
FatI CATG 3 cut(s) 214, 556, 715
FbaI TGATCA 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 541, 585
FokI GGATG 3 cut(s) 666, 887, 901
Fsp4HI GCNGC 2 cut(s) 541, 585
FspBI CTAG 3 cut(s) 354, 512, 923
GlaI GCGC 1 cut(s) 502
GluI GCNGC 2 cut(s) 541, 585
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 2 cut(s) 296, 383
HgaI GACGC 2 cut(s) 621, 708
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 2 cut(s) 501, 613
Hin1II CATG 3 cut(s) 218, 560, 719
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HincII GTYRAC 1 cut(s) 965
HindII GTYRAC 1 cut(s) 965
HinfI GANTC 2 cut(s) 902, 940
HphI GGTGA 2 cut(s) 155, 704
Hpy166II GTNNAC 3 cut(s) 291, 771, 965
Hpy188III TCNNGA 6 cut(s) 223, 322, 530, 869, 923, 944
Hpy8I GTNNAC 3 cut(s) 291, 771, 965
Hpy99I CGWCG 2 cut(s) 183, 615
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 2 cut(s) 167, 768
HpyCH4IV ACGT 2 cut(s) 157, 403
HpyCH4V TGCA 5 cut(s) 151, 256, 428, 491, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 192, 622
HpySE526I ACGT 2 cut(s) 157, 403
Hsp92I GRCGYC 2 cut(s) 501, 613
Hsp92II CATG 3 cut(s) 218, 560, 719
HspAI GCGC 1 cut(s) 501
KasI GGCGCC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 411
Kzo9I GATC 3 cut(s) 303, 411, 422
LmnI GCTCC 1 cut(s) 631
LpnPI CCDG 6 cut(s) 95, 405, 432, 822, 897, 906
Lsp1109I GCAGC 2 cut(s) 552, 596
LweI GCATC 2 cut(s) 138, 160
MaeI CTAG 3 cut(s) 354, 512, 923
MaeII ACGT 2 cut(s) 157, 403
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 305, 413, 424
MbiI CCGCTC 1 cut(s) 980
MboI GATC 3 cut(s) 303, 411, 422
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 8 cut(s) 60, 71, 251, 561, 617, 774, 818, 878
Mly113I GGCGCC 1 cut(s) 501
MlyI GAGTC 1 cut(s) 911
Mph1103I ATGCAT 1 cut(s) 153
MseI TTAA 5 cut(s) 128, 474, 759, 780, 1012
MspR9I CCNGG 1 cut(s) 420
MunI CAATTG 1 cut(s) 251
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 110
NarI GGCGCC 1 cut(s) 501
NdeII GATC 3 cut(s) 303, 411, 422
NlaIII CATG 3 cut(s) 218, 560, 719
NlaIV GGNNCC 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 153
PceI AGGCCT 1 cut(s) 383
PcsI WCGNNNNNNNCGW 3 cut(s) 163, 640, 682
PfeI GAWTC 1 cut(s) 940
PkrI GCNGC 2 cut(s) 542, 586
PleI GAGTC 1 cut(s) 910
PluTI GGCGCC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 910
Ppu21I YACGTR 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 502
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SaqAI TTAA 5 cut(s) 128, 474, 759, 780, 1012
SatI GCNGC 2 cut(s) 541, 585
Sau3AI GATC 3 cut(s) 303, 411, 422
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 2 cut(s) 138, 160
SfcI CTRYAG 2 cut(s) 393, 426
SfoI GGCGCC 1 cut(s) 502
SpeI ACTAGT 1 cut(s) 353
Sse9I AATT 8 cut(s) 60, 71, 251, 561, 617, 774, 818, 878
SseBI AGGCCT 1 cut(s) 383
SsiI CCGC 2 cut(s) 786, 980
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 3 cut(s) 354, 512, 923
StuI AGGCCT 1 cut(s) 383
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 2 cut(s) 167, 768
TaiI ACGT 2 cut(s) 160, 406
TaqI TCGA 6 cut(s) 247, 306, 495, 610, 643, 685
TasI AATT 8 cut(s) 60, 71, 251, 561, 617, 774, 818, 878
TfiI GAWTC 1 cut(s) 940
Tru1I TTAA 5 cut(s) 128, 474, 759, 780, 1012
Tru9I TTAA 5 cut(s) 128, 474, 759, 780, 1012
TscAI CASTG 1 cut(s) 435
TseI GCWGC 2 cut(s) 540, 584
TspDTI ATGAA 3 cut(s) 66, 891, 903
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 1 cut(s) 435
XapI RAATTY 3 cut(s) 71, 561, 878
XbaI TCTAGA 1 cut(s) 922
XspI CTAG 3 cut(s) 354, 512, 923
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.