AT5G45160

root hair defective 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000525
Physical Location & Seq
Forward (+)
18264616 .. 18270296
5681 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45160.1

Sequence Viewer

Length: 2505 bp
ATGGGGGAAAACGATGATGGTTGCTCGACTCAGCTAATCGATGGGAATGGTGAATTTAACGTTAAGGGTTTGGATAATTTCGTGAAAAAGACGAAGCTTTCTGATTGTGGTCTTTCTTATGCTGTTGTTGCAATCATGGGTCCTCAAAGTAGTGGGAAGAGTACACTTTTGAATCATCTTTTTAAAACCAGTTTTCGGGAAATGGATGCTTTTGCGGGAAGGAGTCAAACAACTAAAGGTATTTGGATGGCTAGGTGTGTTGGTATTGAGCCTTTCACAATTGCTATGGATTTGGAGGGTACTGATGGGAGAGAAAGAGGCGAGGATGACACTACATTTGAGAAACAAAGTGCCCTGTTTGCTATAGCTGTTGCCGATATTGTACTGATAAACATGTGGTGCCATGACATTGGAAGGGAACAGGCTGCCAACAAACCATTGCTAAAGACAGTTTTCCAGGTCATGCTGCGATTGTTTAGCCCTCGTAAAACAACTCTTCTTTTTGTCATACGTGATAAAACAAAGACTCCAATAGAGCTGCTTGAACGTGCTCTAAGAGAAGATATTCAGAAGATATGGGATTCAGTTCGCAAGCCTGAAGCACACAAAAATACACCTCTAAATGAATTCTTCAATGTAATGATTGTTGCATTATCCAGCTATGAAGAGAAAGAAAAACAGTTTGAGCAAGAGGTTGCTGAGTTGAGGCAGAGGTTTTTCCATTCCATTTCACCTGGGGGACTTGCTGGTGATAGACGTGGTGTAGTTCCTGCTTCAGGATTTTCTTTCAGCTCCCAGCAGATTTGGAAAGTTATAAAAGAGAATAGAGATTTGGACCTTCCTGCTCACAAGGTAATGGTTGCCACTGTTCGGTGTGAAGAGATAGCCAATGAGAAGCTGCGCGACTTGGCAACCAATGAGAGTTGGTTAGAGTTGCATGAAGCCGCTGAAGGTGGTCTAGTTCCTGGTTTTGGCAAAAAGCTTAGCTCTATCCTTGAAAAATACTTCTCAGAATATGACGCAGAGGCAATATACTTTGATGAAGGAGTAAGAAAAGAGAAGCGTCTACAGCTGAAACTTAATGCTTTGGATTTCGTATACCCTTCTTATGCTACCATGTTGGGACATCTACGGTCCAATGCACTGGAATCCTTTAAGATCCGACTGGAACAGTCCCTAAATCAAGGTGAAGGATTCGCAAAAGCTGTGCGAGACTCCCAACAGTCTTGTCTGATGGTGTTTGACAAAGGATGTGAAGATGCTGCGGTTAAACAAGCAACTTGGGATGCATCAAAAATCAGGGAGAAACTTTGTCGTGACATTGATGCTCACACATTTTTTGCTAGGAGTGCTAAACTATCTGAATTAACTGCCAACTATGAGAAACGGCTAACTCAAGCACTTAGTGAACCTGTTGAGTCTTTGTTCGAAGCTGGTGGAAAAGAGACCTGGCCTTCCATAAGGAAGCTTCTCAAACGCGAGACTGAAACAGCTGTTACAGATTTTCTGGATGTGGTTACTGGTTTTGAGCTAGATCACGCTAAAATTGATGCTATGGTTCAGAATCTAAAGAACTATTCTCAGAGTTTAGTGGAGAAAAAAGCAAGAGAAGAAGCTGCTAAAATTTTAATCCGAATGAAAGATAGGTTCTCAACAGTCTTCAGTCATGACAAAGACTCAATGCCACGGGTCTGGACTGGAAAAGAGGATATTAGAGCAATCACAAAAGATGCTCGAGCAGAGGCTTTAAGTCTTTTATCTGTGATGACTGCAATACGTTTGGATGAAAGACCAGACAATATTGAGAGTACTCTTTTCTCTTCTCTAATGGATGGAACTGTTTCTGCTGCATCTTCCCACAACAGAAGCGTAGGAACGTCTACAGATCCACTTGCATCTAGCTCTTGGGAAGAGGTTCCTCCAAATAATATACTACTGACGCCAGTGCAGTGTAAGTCACTGTGGAGACAGTTCAAATCTGAGACCGAGTATACTGTAACCCAAGCTATTTCGGCACAGGAGGCTCACAAGCGAAATAACAACTGGCTTCCACCGGCTTGGGCGATCGTGTTGATGATTGTCCTTGGTTTCAATGAATTCATGATGCTTTTGAAGAATCCTTTGTACCTTTTGGGTTTCTTCGTGGCGTTTTTACTCTCCAAAGCCTTATGGGTGCAGCTTGACATTCCGAGGGAATTTCAACATGGCGCAGTTGCTGGAGTACTGTCCATCACGTCGAAGTTTCTTCCAACTGTTATGAATCTTCTAAGAAAGCTTGCGGAAGAAGCGCAAGGAAAAACAACACAGGAAGTTCCAGACTTATCAGCTTCCCAAACCTATAGACAGCAATCTCCATCACACTCCATCTCAAGCACAATATCTGAATCTGTCGCATCAAACATATCATCTGCTGGTGATGATGCAGAATACTCAAGCCCAAGCCCGGCCCTGGTTCGGAGACGAAACACCAACAATGTACAAGAATCAGAAATCTCACAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

834

Amino Acids

93.57

Weight (kDa)

5.94

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
URGCP_GTPase PF25683 39 - 170 9e-07 URGCP-like GTPase domain
RHD3_GTPase PF05879 46 - 281 2.2e-115 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 323 - 762 9e-163 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 399
AccI GTMKAC 4 cut(s) 1066, 1098, 1880, 1991
AccII CGCG 2 cut(s) 903, 1479
AciI CCGC 4 cut(s) 215, 945, 1265, 2279
AclI AACGTT 1 cut(s) 60
AclWI GGATC 2 cut(s) 1153, 1880
AcsI RAATTY 5 cut(s) 53, 626, 1623, 2096, 2195
AcuI CTGAAG 4 cut(s) 618, 759, 969, 1645
AcyI GRCGYC 1 cut(s) 1940
AdeI CACNNNGTG 1 cut(s) 1406
AfaI GTAC 7 cut(s) 163, 301, 384, 1810, 2126, 2223, 2478
AfiI CCNNNNNNNGG 7 cut(s) 195, 776, 870, 971, 2443, 2449, 2454
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 8 cut(s) 172, 545, 634, 998, 1975, 2092, 2113, 2201
AjiI CACGTC 2 cut(s) 758, 2235
AjnI CCWGG 5 cut(s) 456, 733, 964, 1448, 2448
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 6 cut(s) 1206, 1439, 1475, 1960, 1976, 2452
AlwI GGATC 2 cut(s) 1153, 1880
AlwNI CAGNNNCTG 1 cut(s) 2216
Ama87I CYCGRG 1 cut(s) 1734
AoxI GGCC 2 cut(s) 1451, 2445
ApeKI GCWGC 8 cut(s) 425, 466, 538, 898, 1262, 1616, 1847, 2176
ApoI RAATTY 5 cut(s) 53, 626, 1623, 2096, 2195
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 3 cut(s) 564, 1840, 1914
AspLEI GCGC 3 cut(s) 903, 2210, 2290
AspS9I GGNCC 4 cut(s) 140, 835, 1134, 2446
AsuC2I CCSGG 1 cut(s) 2444
AsuHPI GGTGA 5 cut(s) 62, 723, 761, 1199, 2426
AsuII TTCGAA 1 cut(s) 1428
AvaI CYCGRG 1 cut(s) 1734
AvaII GGWCC 3 cut(s) 140, 835, 1134
BaeGI GKGCMC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 1651
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 8 cut(s) 412, 453, 525, 885, 1249, 1603, 1834, 2188
BccI CCATC 9 cut(s) 11, 35, 241, 299, 1228, 1826, 2237, 2362, 2372
BceAI ACGGC 1 cut(s) 1403
BciT130I CCWGG 5 cut(s) 458, 735, 966, 1450, 2450
BcnI CCSGG 1 cut(s) 2444
BcoDI GTCTC 6 cut(s) 1206, 1439, 1475, 1960, 1976, 2452
BfaI CTAG 5 cut(s) 252, 959, 1344, 1532, 1899
BfmI CTRYAG 4 cut(s) 363, 1067, 1881, 2338
BisI GCNGC 9 cut(s) 426, 467, 539, 899, 945, 1263, 1617, 1848, 2177
BlpI GCTNAGC 1 cut(s) 983
BlsI GCNGC 9 cut(s) 427, 468, 540, 900, 946, 1264, 1618, 1849, 2178
BmcAI AGTACT 2 cut(s) 1810, 2223
Bme1390I CCNGG 6 cut(s) 458, 735, 966, 1450, 2444, 2450
Bme18I GGWCC 3 cut(s) 140, 835, 1134
BmeT110I CYCGRG 1 cut(s) 1734
BmgBI CACGTC 2 cut(s) 758, 2235
BmgT120I GGNCC 4 cut(s) 140, 835, 1134, 2446
BmiI GGNNCC 3 cut(s) 141, 401, 1917
BmrFI CCNGG 6 cut(s) 458, 735, 966, 1450, 2444, 2450
BpiI GAAGAC 1 cut(s) 1651
BpmI CTGGAG 1 cut(s) 2238
Bpu1102I GCTNAGC 1 cut(s) 983
Bpu14I TTCGAA 1 cut(s) 1428
BpuEI CTTGAG 3 cut(s) 1380, 2353, 2416
BpuMI CCSGG 1 cut(s) 2444
Bsa29I ATCGAT 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 512
BsaHI GRCGYC 1 cut(s) 1940
BsaI GGTCTC 2 cut(s) 1439, 1976
BsaJI CCNNGG 5 cut(s) 734, 1685, 2083, 2189, 2448
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 7 cut(s) 195, 776, 870, 971, 2443, 2449, 2454
Bse118I RCCGGY 1 cut(s) 2053
Bse1I ACTGG 7 cut(s) 189, 1149, 1170, 1525, 1702, 1943, 2048
Bse3DI GCAATG 1 cut(s) 437
BseBI CCWGG 5 cut(s) 458, 735, 966, 1450, 2450
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 5 cut(s) 734, 1685, 2083, 2189, 2448
BseGI GGATG 8 cut(s) 211, 252, 331, 1256, 1291, 1516, 1789, 1837
BseLI CCNNNNNNNGG 7 cut(s) 195, 776, 870, 971, 2443, 2449, 2454
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 5 cut(s) 44, 690, 1023, 1595, 1971
BseNI ACTGG 7 cut(s) 189, 1149, 1170, 1525, 1702, 1943, 2048
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 8 cut(s) 412, 453, 525, 885, 1249, 1603, 1834, 2188
BseYI CCCAGC 1 cut(s) 795
BsgI GTGCAG 2 cut(s) 1967, 2195
Bsh1236I CGCG 2 cut(s) 903, 1479
Bsh1285I CGRYCG 1 cut(s) 2067
BshFI GGCC 2 cut(s) 1453, 2447
BshNI GGYRCC 1 cut(s) 399
BshVI ATCGAT 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 2067
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 1734
BsiSI CCGG 2 cut(s) 2054, 2444
BslFI GGGAC 3 cut(s) 753, 1137, 1159
BslI CCNNNNNNNGG 7 cut(s) 195, 776, 870, 971, 2443, 2449, 2454
BsmAI GTCTC 6 cut(s) 1206, 1439, 1475, 1960, 1976, 2452
BsmBI CGTCTC 1 cut(s) 2452
BsmFI GGGAC 3 cut(s) 753, 1137, 1159
BsnI GGCC 2 cut(s) 1453, 2447
Bso31I GGTCTC 2 cut(s) 1439, 1976
BsoBI CYCGRG 1 cut(s) 1734
Bsp119I TTCGAA 1 cut(s) 1428
Bsp1286I GDGCHC 2 cut(s) 355, 553
Bsp1407I TGTACA 1 cut(s) 2476
Bsp143I GATC 4 cut(s) 1158, 1534, 1885, 2064
Bsp1720I GCTNAGC 1 cut(s) 983
BspACI CCGC 4 cut(s) 215, 945, 1265, 2279
BspANI GGCC 2 cut(s) 1453, 2447
BspCNI CTCAG 5 cut(s) 43, 691, 1022, 1594, 1972
BspDI ATCGAT 1 cut(s) 39
BspFNI CGCG 2 cut(s) 903, 1479
BspHI TCATGA 2 cut(s) 1666, 2100
BspLI GGNNCC 3 cut(s) 141, 401, 1917
BspPI GGATC 2 cut(s) 1153, 1880
BspT104I TTCGAA 1 cut(s) 1428
BspT107I GGYRCC 1 cut(s) 399
BspTNI GGTCTC 2 cut(s) 1439, 1976
BsrDI GCAATG 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 2053
BsrGI TGTACA 1 cut(s) 2476
BsrI ACTGG 7 cut(s) 189, 1149, 1170, 1525, 1702, 1943, 2048
BssAI RCCGGY 1 cut(s) 2053
BssECI CCNNGG 5 cut(s) 734, 1685, 2083, 2189, 2448
BssMI GATC 4 cut(s) 1158, 1534, 1885, 2064
BssNAI GTATAC 2 cut(s) 1099, 1992
BssNI GRCGYC 1 cut(s) 1940
BssT1I CCWWGG 1 cut(s) 2083
Bst1107I GTATAC 2 cut(s) 1099, 1992
Bst2UI CCWGG 5 cut(s) 458, 735, 966, 1450, 2450
Bst6I CTCTTC 6 cut(s) 152, 501, 660, 873, 1825, 1905
BstACI GRCGYC 1 cut(s) 1940
BstAUI TGTACA 1 cut(s) 2476
BstBAI YACGTR 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 1428
BstC8I GCNNGC 2 cut(s) 593, 2277
BstDEI CTNAG 9 cut(s) 30, 554, 699, 983, 1009, 1403, 1581, 1980, 2267
BstDSI CCRYGG 1 cut(s) 1685
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 8 cut(s) 211, 252, 331, 1256, 1291, 1516, 1789, 1837
BstFNI CGCG 2 cut(s) 903, 1479
BstHHI GCGC 3 cut(s) 903, 2210, 2290
BstKTI GATC 4 cut(s) 1161, 1537, 1888, 2067
BstMAI GTCTC 6 cut(s) 1206, 1439, 1475, 1960, 1976, 2452
BstMBI GATC 4 cut(s) 1158, 1534, 1885, 2064
BstMCI CGRYCG 1 cut(s) 2067
BstMWI GCNNNNNNNGC 7 cut(s) 128, 359, 1069, 1349, 2012, 2021, 2285
BstNI CCWGG 5 cut(s) 458, 735, 966, 1450, 2450
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 6 cut(s) 456, 733, 964, 1448, 2442, 2448
BstSFI CTRYAG 4 cut(s) 363, 1067, 1881, 2338
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 2 cut(s) 903, 1479
BstV1I GCAGC 8 cut(s) 412, 453, 525, 885, 1249, 1603, 1834, 2188
BstV2I GAAGAC 1 cut(s) 1651
BstX2I RGATCY 2 cut(s) 1158, 1885
BstXI CCANNNNNNTGG 4 cut(s) 410, 1144, 1692, 2058
BstYI RGATCY 2 cut(s) 1158, 1885
BstZ17I GTATAC 2 cut(s) 1099, 1992
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 2 cut(s) 1453, 2447
BsuTUI ATCGAT 1 cut(s) 39
BtgI CCRYGG 1 cut(s) 1685
BtrI CACGTC 2 cut(s) 758, 2235
BtsCI GGATG 8 cut(s) 211, 252, 331, 1256, 1291, 1516, 1789, 1837
BtsI GCAGTG 1 cut(s) 1955
BtsIMutI CAGTG 5 cut(s) 864, 1142, 1950, 1955, 1958
Cac8I GCNNGC 2 cut(s) 593, 2277
CaiI CAGNNNCTG 1 cut(s) 2216
CciI TCATGA 2 cut(s) 1666, 2100
CfoI GCGC 3 cut(s) 903, 2210, 2290
Cfr10I RCCGGY 1 cut(s) 2053
Cfr13I GGNCC 4 cut(s) 140, 835, 1134, 2446
ClaI ATCGAT 1 cut(s) 39
CseI GACGC 3 cut(s) 1028, 1052, 1948
Csp6I GTAC 7 cut(s) 162, 300, 383, 1809, 2125, 2222, 2477
CviAII CATG 9 cut(s) 136, 394, 404, 463, 938, 1117, 1667, 2101, 2204
CviQI GTAC 7 cut(s) 162, 300, 383, 1809, 2125, 2222, 2477
DdeI CTNAG 9 cut(s) 30, 554, 699, 983, 1009, 1403, 1581, 1980, 2267
DpnI GATC 4 cut(s) 1160, 1536, 1887, 2066
DpnII GATC 4 cut(s) 1158, 1534, 1885, 2064
DraI TTTAAA 1 cut(s) 184
DraIII CACNNNGTG 1 cut(s) 1406
Eam1104I CTCTTC 6 cut(s) 152, 501, 660, 873, 1825, 1905
EarI CTCTTC 6 cut(s) 152, 501, 660, 873, 1825, 1905
Eco130I CCWWGG 1 cut(s) 2083
Eco31I GGTCTC 2 cut(s) 1439, 1976
Eco47I GGWCC 3 cut(s) 140, 835, 1134
Eco57I CTGAAG 4 cut(s) 618, 759, 969, 1645
Eco88I CYCGRG 1 cut(s) 1734
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 140
EcoRI GAATTC 2 cut(s) 626, 2096
EcoRII CCWGG 5 cut(s) 456, 733, 964, 1448, 2448
EcoT14I CCWWGG 1 cut(s) 2083
EcoT22I ATGCAT 1 cut(s) 1291
ErhI CCWWGG 1 cut(s) 2083
Esp3I CGTCTC 1 cut(s) 2452
FaeI CATG 9 cut(s) 139, 397, 407, 466, 941, 1120, 1670, 2104, 2207
FaqI GGGAC 3 cut(s) 753, 1137, 1159
FatI CATG 9 cut(s) 135, 393, 403, 462, 937, 1116, 1666, 2100, 2203
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 4 cut(s) 1066, 1098, 1880, 1991
Fnu4HI GCNGC 9 cut(s) 426, 467, 539, 899, 945, 1263, 1617, 1848, 2177
FokI GGATG 8 cut(s) 218, 259, 338, 1263, 1298, 1523, 1796, 1844
Fsp4HI GCNGC 9 cut(s) 426, 467, 539, 899, 945, 1263, 1617, 1848, 2177
FspBI CTAG 5 cut(s) 252, 959, 1344, 1532, 1899
GlaI GCGC 3 cut(s) 902, 2209, 2289
GluI GCNGC 9 cut(s) 426, 467, 539, 899, 945, 1263, 1617, 1848, 2177
GsaI CCCAGC 1 cut(s) 799
GsuI CTGGAG 1 cut(s) 2238
HaeIII GGCC 2 cut(s) 1453, 2447
HapII CCGG 2 cut(s) 2054, 2444
HgaI GACGC 3 cut(s) 1028, 1052, 1948
HhaI GCGC 3 cut(s) 903, 2210, 2290
Hin1I GRCGYC 1 cut(s) 1940
Hin1II CATG 9 cut(s) 139, 397, 407, 466, 941, 1120, 1670, 2104, 2207
Hin6I GCGC 3 cut(s) 901, 2208, 2288
HinP1I GCGC 3 cut(s) 901, 2208, 2288
HindIII AAGCTT 4 cut(s) 95, 980, 1466, 2273
HpaII CCGG 2 cut(s) 2054, 2444
HphI GGTGA 5 cut(s) 62, 723, 761, 1199, 2426
Hpy166II GTNNAC 6 cut(s) 164, 1067, 1099, 1409, 1881, 1992
Hpy188III TCNNGA 9 cut(s) 82, 197, 777, 1316, 1508, 1667, 1693, 2101, 2315
Hpy8I GTNNAC 6 cut(s) 164, 1067, 1099, 1409, 1881, 1992
Hpy99I CGWCG 1 cut(s) 2239
HpyAV CCTTC 8 cut(s) 213, 408, 848, 944, 1037, 1113, 1184, 1464
HpyCH4IV ACGT 7 cut(s) 60, 511, 547, 757, 1777, 1877, 2234
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 359, 1069, 1349, 2012, 2021, 2285
HpyF3I CTNAG 9 cut(s) 30, 554, 699, 983, 1009, 1403, 1581, 1980, 2267
HpySE526I ACGT 7 cut(s) 60, 511, 547, 757, 1777, 1877, 2234
Hsp92I GRCGYC 1 cut(s) 1940
Hsp92II CATG 9 cut(s) 139, 397, 407, 466, 941, 1120, 1670, 2104, 2207
HspAI GCGC 3 cut(s) 901, 2208, 2288
Kzo9I GATC 4 cut(s) 1158, 1534, 1885, 2064
LmnI GCTCC 1 cut(s) 797
Lsp1109I GCAGC 8 cut(s) 412, 453, 525, 885, 1249, 1603, 1834, 2188
MaeI CTAG 5 cut(s) 252, 959, 1344, 1532, 1899
MaeII ACGT 7 cut(s) 60, 511, 547, 757, 1777, 1877, 2234
MaeIII GTNAC 5 cut(s) 1316, 1495, 1516, 1956, 1996
MalI GATC 4 cut(s) 1160, 1536, 1887, 2066
MboI GATC 4 cut(s) 1158, 1534, 1885, 2064
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 2 cut(s) 1158, 1885
MhlI GDGCHC 2 cut(s) 355, 553
MluCI AATT 9 cut(s) 53, 76, 279, 626, 1364, 1545, 1623, 2096, 2195
MlyI GAGTC 6 cut(s) 22, 232, 520, 1208, 1427, 1670
MmeI TCCRAC 2 cut(s) 1186, 2273
Mph1103I ATGCAT 1 cut(s) 1291
MroXI GAANNNNTTC 3 cut(s) 564, 1840, 1914
MseI TTAA 9 cut(s) 57, 63, 183, 1080, 1155, 1269, 1367, 1628, 1748
MspA1I CMGCKG 3 cut(s) 947, 1072, 1493
MspI CCGG 2 cut(s) 2054, 2444
MspR9I CCNGG 6 cut(s) 458, 735, 966, 1450, 2444, 2450
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 5 cut(s) 458, 735, 966, 1450, 2450
MvnI CGCG 2 cut(s) 903, 1479
MwoI GCNNNNNNNGC 7 cut(s) 128, 359, 1069, 1349, 2012, 2021, 2285
NciI CCSGG 1 cut(s) 2444
NdeII GATC 4 cut(s) 1158, 1534, 1885, 2064
NlaIII CATG 9 cut(s) 139, 397, 407, 466, 941, 1120, 1670, 2104, 2207
NlaIV GGNNCC 3 cut(s) 141, 401, 1917
NmuCI GTSAC 2 cut(s) 1316, 1956
NsiI ATGCAT 1 cut(s) 1291
NspI RCATGY 1 cut(s) 397
NspV TTCGAA 1 cut(s) 1428
PaeR7I CTCGAG 1 cut(s) 1734
PagI TCATGA 2 cut(s) 1666, 2100
PciI ACATGT 1 cut(s) 393
PdmI GAANNNNTTC 3 cut(s) 564, 1840, 1914
PfeI GAWTC 9 cut(s) 172, 581, 1148, 1194, 1564, 2116, 2260, 2384, 2483
PkrI GCNGC 9 cut(s) 427, 468, 540, 900, 946, 1264, 1618, 1849, 2178
Ple19I CGATCG 1 cut(s) 2067
PleI GAGTC 6 cut(s) 22, 231, 520, 1208, 1426, 1670
PpsI GAGTC 6 cut(s) 22, 231, 520, 1208, 1426, 1670
Ppu21I YACGTR 1 cut(s) 512
PpuMI RGGWCCY 1 cut(s) 140
PscI ACATGT 1 cut(s) 393
PsiI TTATAA 1 cut(s) 815
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 140
Psp6I CCWGG 5 cut(s) 456, 733, 964, 1448, 2448
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 5 cut(s) 456, 733, 964, 1448, 2448
PspN4I GGNNCC 3 cut(s) 141, 401, 1917
PspPI GGNCC 4 cut(s) 140, 835, 1134, 2446
PspPPI RGGWCCY 1 cut(s) 140
PspXI VCTCGAGB 1 cut(s) 1734
PstNI CAGNNNCTG 1 cut(s) 2216
PsuI RGATCY 2 cut(s) 1158, 1885
PvuI CGATCG 1 cut(s) 2067
PvuII CAGCTG 2 cut(s) 1072, 1493
RsaI GTAC 7 cut(s) 163, 301, 384, 1810, 2126, 2223, 2478
RsaNI GTAC 7 cut(s) 162, 300, 383, 1809, 2125, 2222, 2477
SaqAI TTAA 9 cut(s) 57, 63, 183, 1080, 1155, 1269, 1367, 1628, 1748
SatI GCNGC 9 cut(s) 426, 467, 539, 899, 945, 1263, 1617, 1848, 2177
Sau3AI GATC 4 cut(s) 1158, 1534, 1885, 2064
Sau96I GGNCC 4 cut(s) 140, 835, 1134, 2446
ScaI AGTACT 2 cut(s) 1810, 2223
SchI GAGTC 6 cut(s) 22, 232, 520, 1208, 1427, 1670
ScrFI CCNGG 6 cut(s) 458, 735, 966, 1450, 2444, 2450
SduI GDGCHC 2 cut(s) 355, 553
SfcI CTRYAG 4 cut(s) 363, 1067, 1881, 2338
Sfr274I CTCGAG 1 cut(s) 1734
SfuI TTCGAA 1 cut(s) 1428
SinI GGWCC 3 cut(s) 140, 835, 1134
SlaI CTCGAG 1 cut(s) 1734
SmlI CTYRAG 4 cut(s) 1395, 1734, 2368, 2431
SmoI CTYRAG 4 cut(s) 1395, 1734, 2368, 2431
Sse9I AATT 9 cut(s) 53, 76, 279, 626, 1364, 1545, 1623, 2096, 2195
SsiI CCGC 4 cut(s) 215, 945, 1265, 2279
SspI AATATT 1 cut(s) 1801
SspMI CTAG 5 cut(s) 252, 959, 1344, 1532, 1899
StyD4I CCNGG 6 cut(s) 456, 733, 964, 1448, 2442, 2448
StyI CCWWGG 1 cut(s) 2083
TaiI ACGT 7 cut(s) 63, 514, 550, 760, 1780, 1880, 2237
TaqI TCGA 5 cut(s) 26, 39, 1428, 1735, 2237
TaqII GACCGA 1 cut(s) 2000
TasI AATT 9 cut(s) 53, 76, 279, 626, 1364, 1545, 1623, 2096, 2195
TatI WGTACW 5 cut(s) 161, 382, 1808, 2221, 2476
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 9 cut(s) 172, 581, 1148, 1194, 1564, 2116, 2260, 2384, 2483
Tru1I TTAA 9 cut(s) 57, 63, 183, 1080, 1155, 1269, 1367, 1628, 1748
Tru9I TTAA 9 cut(s) 57, 63, 183, 1080, 1155, 1269, 1367, 1628, 1748
TscAI CASTG 5 cut(s) 871, 1149, 1950, 1955, 1965
TseFI GTSAC 2 cut(s) 1316, 1956
TseI GCWGC 8 cut(s) 425, 466, 538, 898, 1262, 1616, 1847, 2176
Tsp45I GTSAC 2 cut(s) 1316, 1956
TspDTI ATGAA 9 cut(s) 639, 678, 954, 1056, 1652, 1800, 2089, 2109, 2273
TspRI CASTG 5 cut(s) 871, 1149, 1950, 1955, 1965
VpaK11BI GGWCC 3 cut(s) 140, 835, 1134
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 5 cut(s) 53, 626, 1623, 2096, 2195
XceI RCATGY 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 1734
XmiI GTMKAC 4 cut(s) 1066, 1098, 1880, 1991
XmnI GAANNNNTTC 3 cut(s) 564, 1840, 1914
XspI CTAG 5 cut(s) 252, 959, 1344, 1532, 1899
ZrmI AGTACT 2 cut(s) 1810, 2223
Zsp2I ATGCAT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.