Rroxscaffold_1G00074270

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000525
Physical Location & Seq
Reverse (-)
95315963 .. 95322851
6889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074270.1

Sequence Viewer

Length: 2430 bp
ATGGCGGCGGAGGATTGCTACGCCACGCAGCTGATCGACGGCGACGGCGAATTCAATGCCGCCGGGCTGGACCGATTCGTGAAGGAAGCGAAGGTTATCGATTGCGGGCTCTCGTACGCCGTAGTCGCCATTATGGGGCCGCAGAGTAGCGGGAAGAGTACTTTGATGAATCATCTGTTTCATACTAAGTTCAGGGAGATGGATGCGTTTAGGGGAAGGAGCCAAACGACGAAGGGGATTTGGATTGCCAAGTGTGTTGGCATTGAGCCTTGCACTATAGGCATGGATTTGGAGGGTACTGATGGCAGGGAGAGAGGCGAGGATGATACTACATTTGAGAAACAAAGCGCTCTTTTTGCGTTGGCAGTTTCAGACATTGTGCTGATTAACATGTGGTGCCATGATATTGGTCGGGAGCAGGCTGCAAACAAACCTTTACTGAAAACAGTTTTTCAGGTGATGATGCGCTTATTTAGCCCACGTAAAACGACGTTGTTGTTTGTTATACGCGATAAAACAAAGACCCCATTTGAGTATCTGGAACCTGTTTTAAGGGAAGATATACAAAAGATATGGGATTCAGTTCCAAAGCCCCAAGCCCACAAAAGCACCCCGCTCAGCGAATTTTTCACTGTTGCTGAACTGCGGCAGCGTTTTTTCCATTCTATTTCTCCAGGAGGGCTTGCTGGTGATAGACGGGGTGTTGTCCCTGCCTCCGGATTTTCATTTAGCGCACAACGGATTTGGAAAATAATCAAAGAAAACAAAGACCTGGATCTTCCTGCTCACAAGGTTATGGTTGCTACTGTTCGGTGTGAAGAGATTGCTAATGAGAAATTCAATCACTTGACGTTTAATGAGGATTGGTTGGCATTGGAAGAAGCTGTGCAAAGTGGTCCAGTACCAGGTTTTGGTAAACAGCTCAGCTCCATCCTAGACACCTATCTTTCTGAATATGACATGGAGGCCATATACTTTGATGAAGGTGTAAGGAACTCAAGACGACACCAATTGGAAAAAAGAGCATTGGATTTTGTGTTCCCTGCATACACTACCATGCTTGGGCACCTACGTTCTAATGCATTGGAAAATTTTAAAGTGAGATTGGAGCAGTCATTGAACAATGGAGAAAGTTTTGCCTCATCTGTTCGAACCTGTATGGAGGGATGCATGCTTGAGTTTGACCGTGGATGTGCAGATGCTGCTGTACAGCAGGTTAATTGGGATACTTCAAGAGTTCGGGAAAAGCTCCGACGTGATATAGATGCACATGCATCATCTATTCGTAGTACAAAGTTGTCGGAATTGAATACCAAAAAACTCTCAGCTTCTTTAACTGAACCTGTGGGGGCTCTACTTGATGCCGGAGGAGAAGACACCTGGGCTTCAATAAGGAAACTTCTTAATCATGAGACTGAATCTGTAGTATTAGAGTTCTCAAAAGCAGTTGTTGGTTTTGAGTTGGATAAAGTAACAATTGCGAAAATGGTGCAAAATTTAAGGGATTATGCAAGAAATGTAGTGGAGATAAAAGCAAGAGAAGAGGCTGGCAAAGTTCTGATCCACATGAAGGATCGGTTTTCAACAGTCTTCAATTATGACAGTGATTCAATGCCTAGGATATGGACCGGGAAGGAAGACATTAAAACCATAACCAAGGATGCCCGCTCTGCGTCTCTCAAGATTTTATCGGTTAGAGCTGCTATTCGCTTGGATGAGAAGCCTGATAATATTGAAAAAGTGATCTTTTCTTCTCTAATGGATGGAACTGGTACTGCTTTGTCCTCTCAAGATACGAGAACAAGAGCTTTAGCAGATCCTCTTGCAACAAGCACTTGGGAGGAGGTTTCTCCAAAGGATACACTGATTACACCAGTGCAATGCAAATCACTGTGGAGACAGTTCAAATCAGAAACCGAGTATACTATCACTCAAGCTATTTCAGCACAGGAGGCCCACAAGCGTAGTAACAACTGGTTGCCTCCTCCATGGGCCATTATGGCAATGATTCTTCTTGGTTTCAATGAATTTATGCTCCTTTTAAGGAACCCTCTCTACCTCTTGGTTCTTTTTGTTGCATATTTACTTACGAAGGCCTTATGGGTACAGATGGACATTGCGGGAGAGTTCCGACATGGCGCTCTTTCAGGAATATTATCCATTTCATCGAAGTTTCTTCCCACTGTTATGGATATCCTGAGACGGCTTGCAGAAGAAGCTCAAGGGCGTCCAACACCTGAAGCGCCAAGACAGCCAGTTTCTCTTGCTTCTCAAAGTTATAGAAGTGAAACACCTCAGCCAAATCCAGTGACGAGCTCAGTTCCTGAGTCATCCGTATCATCTAATATTTCATCTTCTGATAGTGGCATGGAATGCTCGAGCCCTAACTTGACACACAGGCGAACCACAAACATTCAGGAGATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

809

Amino Acids

90.72

Weight (kDa)

6.11

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RHD3_GTPase PF05879 45 - 212 1.4e-83 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
RHD3_GTPase PF05879 212 - 261 1.1e-13 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 303 - 739 1.4e-134 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1204
AccB1I GGYRCC 2 cut(s) 396, 1065
AccB7I CCANNNNNTGG 1 cut(s) 911
AccBSI CCGCTC 2 cut(s) 616, 1668
AccI GTMKAC 1 cut(s) 1922
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 716
AclWI GGATC 4 cut(s) 783, 1555, 1581, 1811
AcsI RAATTY 6 cut(s) 50, 623, 836, 1090, 1495, 2027
AcuI CTGAAG 1 cut(s) 2259
AcyI GRCGYC 1 cut(s) 2227
AfaI GTAC 8 cut(s) 116, 160, 298, 903, 1209, 1291, 1774, 2106
AfeI AGCGCT 1 cut(s) 349
AfiI CCNNNNNNNGG 6 cut(s) 135, 716, 905, 911, 1062, 1570
AflIII ACRYGT 1 cut(s) 390
AjiI CACGTC 1 cut(s) 1256
AjnI CCWGG 4 cut(s) 673, 771, 904, 1379
AloI GAACNNNNNNTCC 2 cut(s) 1022, 1054
Alw21I GWGCWC 1 cut(s) 2318
Alw26I GTCTC 4 cut(s) 1406, 1680, 1891, 2194
AlwI GGATC 4 cut(s) 783, 1555, 1581, 1811
AlwNI CAGNNNCTG 2 cut(s) 1202, 2324
Ama87I CYCGRG 1 cut(s) 2377
Aor13HI TCCGGA 1 cut(s) 716
Aor51HI AGCGCT 1 cut(s) 349
AoxI GGCC 5 cut(s) 137, 966, 1953, 1992, 2094
ApeKI GCWGC 5 cut(s) 28, 422, 649, 1202, 1700
ApoI RAATTY 6 cut(s) 50, 623, 836, 1090, 1495, 2027
AspA2I CCTAGG 1 cut(s) 1616
AspLEI GCGC 5 cut(s) 350, 468, 734, 2141, 2245
AspS9I GGNCC 6 cut(s) 70, 137, 896, 1626, 1954, 1992
AsuC2I CCSGG 2 cut(s) 64, 1630
AsuHPI GGTGA 2 cut(s) 469, 701
AsuII TTCGAA 1 cut(s) 1150
AvaI CYCGRG 1 cut(s) 2377
AvaII GGWCC 3 cut(s) 70, 896, 1626
AvrII CCTAGG 1 cut(s) 1616
BaeGI GKGCMC 1 cut(s) 1068
BanI GGYRCC 2 cut(s) 396, 1065
BanII GRGCYC 4 cut(s) 111, 1354, 2318, 2384
BbsI GAAGAC 3 cut(s) 1380, 1582, 1644
Bbv12I GWGCWC 1 cut(s) 2318
BbvCI CCTCAGC 1 cut(s) 2295
BbvI GCAGC 5 cut(s) 40, 409, 661, 1189, 1687
BccI CCATC 6 cut(s) 193, 296, 938, 1757, 2104, 2416
BceAI ACGGC 4 cut(s) 55, 61, 104, 2219
BcgI CGANNNNNNTGC 4 cut(s) 38, 72, 1471, 1505
BciT130I CCWGG 4 cut(s) 675, 773, 906, 1381
BciVI GTATCC 2 cut(s) 1219, 1852
BcnI CCSGG 2 cut(s) 64, 1630
BcoDI GTCTC 4 cut(s) 1406, 1680, 1891, 2194
BfaI CTAG 2 cut(s) 935, 1617
BfmI CTRYAG 2 cut(s) 276, 1422
BfoI RGCGCY 3 cut(s) 351, 2142, 2246
BfuAI ACCTGC 1 cut(s) 1204
BfuI GTATCC 2 cut(s) 1219, 1852
BglI GCCNNNNNGGC 1 cut(s) 2000
BisI GCNGC 9 cut(s) 6, 29, 60, 140, 423, 647, 650, 1203, 1701
BlnI CCTAGG 1 cut(s) 1616
BlpI GCTNAGC 2 cut(s) 617, 923
BlsI GCNGC 9 cut(s) 7, 30, 61, 141, 424, 648, 651, 1204, 1702
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 6 cut(s) 64, 675, 773, 906, 1381, 1630
Bme18I GGWCC 3 cut(s) 70, 896, 1626
BmeT110I CYCGRG 1 cut(s) 2377
BmgBI CACGTC 1 cut(s) 1256
BmgT120I GGNCC 6 cut(s) 70, 137, 896, 1626, 1954, 1992
BmiI GGNNCC 6 cut(s) 138, 221, 398, 543, 1067, 2048
BmrFI CCNGG 6 cut(s) 64, 675, 773, 906, 1381, 1630
BmsI GCATC 8 cut(s) 193, 453, 1157, 1189, 1255, 1283, 1351, 1651
BpiI GAAGAC 3 cut(s) 1380, 1582, 1644
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 2295
Bpu1102I GCTNAGC 2 cut(s) 617, 923
Bpu14I TTCGAA 1 cut(s) 1150
BpuEI CTTGAG 6 cut(s) 982, 1196, 1664, 1773, 1917, 2205
BpuMI CCSGG 2 cut(s) 64, 1630
Bsa29I ATCGAT 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 2227
BsaJI CCNNGG 5 cut(s) 1186, 1380, 1616, 1656, 1988
BsaWI WCCGGW 1 cut(s) 716
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 716, 905, 911, 1062, 1570
Bse1I ACTGG 6 cut(s) 899, 1774, 1874, 1979, 2255, 2306
Bse3DI GCAATG 3 cut(s) 1886, 2010, 2115
BseAI TCCGGA 1 cut(s) 716
BseBI CCWGG 4 cut(s) 675, 773, 906, 1381
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 5 cut(s) 1186, 1380, 1616, 1656, 1988
BseGI GGATG 9 cut(s) 208, 328, 930, 1172, 1196, 1666, 1720, 1768, 2330
BseLI CCNNNNNNNGG 6 cut(s) 135, 716, 905, 911, 1062, 1570
BseMI GCAATG 3 cut(s) 1886, 2010, 2115
BseMII CTCAG 7 cut(s) 631, 937, 1338, 2189, 2309, 2316, 2331
BseNI ACTGG 6 cut(s) 899, 1774, 1874, 1979, 2255, 2306
BseRI GAGGAG 3 cut(s) 1383, 1856, 1974
BseSI GKGCMC 1 cut(s) 1068
BseXI GCAGC 5 cut(s) 40, 409, 661, 1189, 1687
BsgI GTGCAG 1 cut(s) 1215
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 5 cut(s) 139, 968, 1955, 1994, 2096
BshNI GGYRCC 2 cut(s) 396, 1065
BshVI ATCGAT 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 2318
BsiHKCI CYCGRG 1 cut(s) 2377
BsiSI CCGG 4 cut(s) 63, 717, 1365, 1629
BsiWI CGTACG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 6 cut(s) 135, 716, 905, 911, 1062, 1570
BsmAI GTCTC 4 cut(s) 1406, 1680, 1891, 2194
BsmBI CGTCTC 2 cut(s) 1680, 2194
BsmFI GGGAC 1 cut(s) 692
BsmI GAATGC 1 cut(s) 2378
BsnI GGCC 5 cut(s) 139, 968, 1955, 1994, 2096
BsoBI CYCGRG 1 cut(s) 2377
Bsp119I TTCGAA 1 cut(s) 1150
Bsp1286I GDGCHC 5 cut(s) 111, 1068, 1354, 2318, 2384
Bsp13I TCCGGA 1 cut(s) 716
Bsp1407I TGTACA 1 cut(s) 1207
Bsp143I GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
Bsp1720I GCTNAGC 2 cut(s) 617, 923
Bsp19I CCATGG 1 cut(s) 1988
BspANI GGCC 5 cut(s) 139, 968, 1955, 1994, 2096
BspCNI CTCAG 7 cut(s) 630, 936, 1337, 2190, 2308, 2317, 2330
BspDI ATCGAT 1 cut(s) 99
BspEI TCCGGA 1 cut(s) 716
BspFNI CGCG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 1408
BspLI GGNNCC 6 cut(s) 138, 221, 398, 543, 1067, 2048
BspMI ACCTGC 1 cut(s) 1204
BspPI GGATC 4 cut(s) 783, 1555, 1581, 1811
BspT104I TTCGAA 1 cut(s) 1150
BspT107I GGYRCC 2 cut(s) 396, 1065
BsrBI CCGCTC 2 cut(s) 616, 1668
BsrDI GCAATG 3 cut(s) 1886, 2010, 2115
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 6 cut(s) 899, 1774, 1874, 1979, 2255, 2306
BssECI CCNNGG 5 cut(s) 1186, 1380, 1616, 1656, 1988
BssMI GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
BssNAI GTATAC 1 cut(s) 1923
BssNI GRCGYC 1 cut(s) 2227
BssT1I CCWWGG 3 cut(s) 1616, 1656, 1988
Bst1107I GTATAC 1 cut(s) 1923
Bst2UI CCWGG 4 cut(s) 675, 773, 906, 1381
Bst4CI ACNGT 9 cut(s) 448, 634, 808, 1187, 1588, 1604, 1893, 1902, 2185
Bst6I CTCTTC 3 cut(s) 149, 813, 1536
BstACI GRCGYC 1 cut(s) 2227
BstAPI GCANNNNNTGC 2 cut(s) 1202, 2373
BstAUI TGTACA 1 cut(s) 1207
BstBAI YACGTR 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 1150
BstC8I GCNNGC 7 cut(s) 107, 420, 684, 1172, 1549, 1666, 2208
BstDEI CTNAG 8 cut(s) 186, 617, 923, 1324, 2198, 2295, 2317, 2325
BstDSI CCRYGG 2 cut(s) 1186, 1988
BstF5I GGATG 9 cut(s) 208, 328, 930, 1172, 1196, 1666, 1720, 1768, 2330
BstFNI CGCG 1 cut(s) 510
BstH2I RGCGCY 3 cut(s) 351, 2142, 2246
BstHHI GCGC 5 cut(s) 350, 468, 734, 2141, 2245
BstKTI GATC 6 cut(s) 36, 778, 1563, 1576, 1746, 1819
BstMAI GTCTC 4 cut(s) 1406, 1680, 1891, 2194
BstMBI GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
BstNI CCWGG 4 cut(s) 675, 773, 906, 1381
BstNSI RCATGY 3 cut(s) 394, 1174, 1274
BstSCI CCNGG 6 cut(s) 62, 673, 771, 904, 1379, 1628
BstSFI CTRYAG 2 cut(s) 276, 1422
BstSLI GKGCMC 1 cut(s) 1068
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 5 cut(s) 40, 409, 661, 1189, 1687
BstV2I GAAGAC 3 cut(s) 1380, 1582, 1644
BstX2I RGATCY 2 cut(s) 775, 1816
BstXI CCANNNNNNTGG 2 cut(s) 407, 2188
BstYI RGATCY 2 cut(s) 775, 1816
BstZ17I GTATAC 1 cut(s) 1923
Bsu15I ATCGAT 1 cut(s) 99
BsuI GTATCC 2 cut(s) 1219, 1852
BsuRI GGCC 5 cut(s) 139, 968, 1955, 1994, 2096
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 1186, 1988
BtrI CACGTC 1 cut(s) 1256
BtsCI GGATG 9 cut(s) 208, 328, 930, 1172, 1196, 1666, 1720, 1768, 2330
BtsIMutI CAGTG 7 cut(s) 630, 1609, 1862, 1881, 1889, 2181, 2313
BveI ACCTGC 1 cut(s) 1204
Cac8I GCNNGC 7 cut(s) 107, 420, 684, 1172, 1549, 1666, 2208
CaiI CAGNNNCTG 2 cut(s) 1202, 2324
CciI TCATGA 1 cut(s) 1408
CfoI GCGC 5 cut(s) 350, 468, 734, 2141, 2245
Cfr13I GGNCC 6 cut(s) 70, 137, 896, 1626, 1954, 1992
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 2 cut(s) 1662, 2216
CsiI ACCWGGT 1 cut(s) 904
Csp6I GTAC 8 cut(s) 115, 159, 297, 902, 1208, 1290, 1773, 2105
CviQI GTAC 8 cut(s) 115, 159, 297, 902, 1208, 1290, 1773, 2105
DdeI CTNAG 8 cut(s) 186, 617, 923, 1324, 2198, 2295, 2317, 2325
DpnI GATC 6 cut(s) 35, 777, 1562, 1575, 1745, 1818
DpnII GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
DraI TTTAAA 1 cut(s) 1096
Eam1104I CTCTTC 3 cut(s) 149, 813, 1536
EarI CTCTTC 3 cut(s) 149, 813, 1536
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 2316
Eco130I CCWWGG 3 cut(s) 1616, 1656, 1988
Eco147I AGGCCT 1 cut(s) 2096
Eco24I GRGCYC 4 cut(s) 111, 1354, 2318, 2384
Eco32I GATATC 1 cut(s) 2194
Eco47I GGWCC 3 cut(s) 70, 896, 1626
Eco47III AGCGCT 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 2316
Eco57I CTGAAG 1 cut(s) 2259
Eco88I CYCGRG 1 cut(s) 2377
EcoICRI GAGCTC 1 cut(s) 2316
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 4 cut(s) 673, 771, 904, 1379
EcoRV GATATC 1 cut(s) 2194
EcoT14I CCWWGG 3 cut(s) 1616, 1656, 1988
EcoT22I ATGCAT 3 cut(s) 1084, 1172, 1276
EcoT38I GRGCYC 4 cut(s) 111, 1354, 2318, 2384
ErhI CCWWGG 3 cut(s) 1616, 1656, 1988
Esp3I CGTCTC 2 cut(s) 1680, 2194
FaqI GGGAC 1 cut(s) 692
FauI CCCGC 5 cut(s) 98, 143, 621, 1673, 2113
FblI GTMKAC 1 cut(s) 1922
Fnu4HI GCNGC 9 cut(s) 6, 29, 60, 140, 423, 647, 650, 1203, 1701
FokI GGATG 9 cut(s) 215, 335, 917, 1179, 1203, 1673, 1727, 1775, 2317
FriOI GRGCYC 4 cut(s) 111, 1354, 2318, 2384
Fsp4HI GCNGC 9 cut(s) 6, 29, 60, 140, 423, 647, 650, 1203, 1701
FspBI CTAG 2 cut(s) 935, 1617
GlaI GCGC 5 cut(s) 349, 467, 733, 2140, 2244
GluI GCNGC 9 cut(s) 6, 29, 60, 140, 423, 647, 650, 1203, 1701
GsuI CTGGAG 1 cut(s) 657
HaeII RGCGCY 3 cut(s) 351, 2142, 2246
HaeIII GGCC 5 cut(s) 139, 968, 1955, 1994, 2096
HapII CCGG 4 cut(s) 63, 717, 1365, 1629
HgaI GACGC 2 cut(s) 1662, 2216
HhaI GCGC 5 cut(s) 350, 468, 734, 2141, 2245
Hin1I GRCGYC 1 cut(s) 2227
Hin6I GCGC 5 cut(s) 348, 466, 732, 2139, 2243
HinP1I GCGC 5 cut(s) 348, 466, 732, 2139, 2243
HinfI GANTC 7 cut(s) 75, 169, 578, 1418, 1607, 2008, 2327
HpaII CCGG 4 cut(s) 63, 717, 1365, 1629
HphI GGTGA 2 cut(s) 469, 701
Hpy166II GTNNAC 2 cut(s) 917, 1923
Hpy188I TCNGA 8 cut(s) 373, 952, 1253, 1303, 1560, 1912, 2132, 2359
Hpy8I GTNNAC 2 cut(s) 917, 1923
Hpy99I CGWCG 5 cut(s) 41, 47, 232, 493, 1257
HpyAV CCTTC 8 cut(s) 76, 85, 210, 226, 977, 1564, 1627, 2086
HpyCH4III ACNGT 9 cut(s) 448, 634, 808, 1187, 1588, 1604, 1893, 1902, 2185
HpyCH4IV ACGT 5 cut(s) 481, 491, 851, 1072, 1255
HpyF3I CTNAG 8 cut(s) 186, 617, 923, 1324, 2198, 2295, 2317, 2325
HpySE526I ACGT 5 cut(s) 481, 491, 851, 1072, 1255
Hsp92I GRCGYC 1 cut(s) 2227
HspAI GCGC 5 cut(s) 348, 466, 732, 2139, 2243
Kpn2I TCCGGA 1 cut(s) 716
Kzo9I GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
LmnI GCTCC 6 cut(s) 219, 415, 932, 1108, 1254, 2040
Lsp1109I GCAGC 5 cut(s) 40, 409, 661, 1189, 1687
LweI GCATC 8 cut(s) 193, 453, 1157, 1189, 1255, 1283, 1351, 1651
MabI ACCWGGT 1 cut(s) 904
MaeI CTAG 2 cut(s) 935, 1617
MaeII ACGT 5 cut(s) 481, 491, 851, 1072, 1255
MaeIII GTNAC 3 cut(s) 1471, 1967, 2308
MalI GATC 6 cut(s) 35, 777, 1562, 1575, 1745, 1818
MbiI CCGCTC 2 cut(s) 616, 1668
MboI GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
MfeI CAATTG 2 cut(s) 1010, 1476
MflI RGATCY 2 cut(s) 775, 1816
MhlI GDGCHC 5 cut(s) 111, 1068, 1354, 2318, 2384
MlyI GAGTC 1 cut(s) 2336
MmeI TCCRAC 5 cut(s) 1276, 1281, 1443, 2155, 2255
Mph1103I ATGCAT 3 cut(s) 1084, 1172, 1276
MroI TCCGGA 1 cut(s) 716
MslI CAYNNNNRTG 2 cut(s) 1055, 2186
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 4 cut(s) 63, 717, 1365, 1629
MspR9I CCNGG 6 cut(s) 64, 675, 773, 906, 1381, 1630
MunI CAATTG 2 cut(s) 1010, 1476
Mva1269I GAATGC 1 cut(s) 2378
MvaI CCWGG 4 cut(s) 675, 773, 906, 1381
MvnI CGCG 1 cut(s) 510
NciI CCSGG 2 cut(s) 64, 1630
NcoI CCATGG 1 cut(s) 1988
NdeII GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
NlaIV GGNNCC 6 cut(s) 138, 221, 398, 543, 1067, 2048
NmuCI GTSAC 1 cut(s) 2308
NsiI ATGCAT 3 cut(s) 1084, 1172, 1276
NspI RCATGY 3 cut(s) 394, 1174, 1274
NspV TTCGAA 1 cut(s) 1150
PaeI GCATGC 1 cut(s) 1174
PaeR7I CTCGAG 1 cut(s) 2377
PagI TCATGA 1 cut(s) 1408
PceI AGGCCT 1 cut(s) 2096
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 2378
PfeI GAWTC 6 cut(s) 75, 169, 578, 1418, 1607, 2008
Pfl23II CGTACG 1 cut(s) 114
PflMI CCANNNNNTGG 1 cut(s) 911
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 9 cut(s) 7, 30, 61, 141, 424, 648, 651, 1204, 1702
PleI GAGTC 1 cut(s) 2335
PpsI GAGTC 1 cut(s) 2335
Ppu21I YACGTR 1 cut(s) 482
PscI ACATGT 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 2318
Psp6I CCWGG 4 cut(s) 673, 771, 904, 1379
PspGI CCWGG 4 cut(s) 673, 771, 904, 1379
PspLI CGTACG 1 cut(s) 114
PspN4I GGNNCC 6 cut(s) 138, 221, 398, 543, 1067, 2048
PspPI GGNCC 6 cut(s) 70, 137, 896, 1626, 1954, 1992
PspXI VCTCGAGB 1 cut(s) 2377
PsrI GAACNNNNNNTAC 2 cut(s) 2039, 2071
PstNI CAGNNNCTG 2 cut(s) 1202, 2324
PsuI RGATCY 2 cut(s) 775, 1816
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 8 cut(s) 116, 160, 298, 903, 1209, 1291, 1774, 2106
RsaNI GTAC 8 cut(s) 115, 159, 297, 902, 1208, 1290, 1773, 2105
RseI CAYNNNNRTG 2 cut(s) 1055, 2186
SacI GAGCTC 1 cut(s) 2318
SatI GCNGC 9 cut(s) 6, 29, 60, 140, 423, 647, 650, 1203, 1701
Sau3AI GATC 6 cut(s) 33, 775, 1560, 1573, 1743, 1816
Sau96I GGNCC 6 cut(s) 70, 137, 896, 1626, 1954, 1992
ScaI AGTACT 1 cut(s) 160
SchI GAGTC 1 cut(s) 2336
ScrFI CCNGG 6 cut(s) 64, 675, 773, 906, 1381, 1630
SduI GDGCHC 5 cut(s) 111, 1068, 1354, 2318, 2384
SexAI ACCWGGT 1 cut(s) 904
SfaNI GCATC 8 cut(s) 193, 453, 1157, 1189, 1255, 1283, 1351, 1651
SfcI CTRYAG 2 cut(s) 276, 1422
Sfr274I CTCGAG 1 cut(s) 2377
SfuI TTCGAA 1 cut(s) 1150
SinI GGWCC 3 cut(s) 70, 896, 1626
SlaI CTCGAG 1 cut(s) 2377
SmiMI CAYNNNNRTG 2 cut(s) 1055, 2186
SmlI CTYRAG 7 cut(s) 997, 1175, 1679, 1788, 1932, 2220, 2377
SmoI CTYRAG 7 cut(s) 997, 1175, 1679, 1788, 1932, 2220, 2377
SphI GCATGC 1 cut(s) 1174
SseBI AGGCCT 1 cut(s) 2096
SspI AATATT 3 cut(s) 1732, 2154, 2347
SspMI CTAG 2 cut(s) 935, 1617
SstI GAGCTC 1 cut(s) 2318
StuI AGGCCT 1 cut(s) 2096
StyD4I CCNGG 6 cut(s) 62, 673, 771, 904, 1379, 1628
StyI CCWWGG 3 cut(s) 1616, 1656, 1988
TaaI ACNGT 9 cut(s) 448, 634, 808, 1187, 1588, 1604, 1893, 1902, 2185
TaiI ACGT 5 cut(s) 484, 494, 854, 1075, 1258
TaqI TCGA 5 cut(s) 36, 99, 1150, 2168, 2378
TaqII GACCGA 1 cut(s) 87
TatI WGTACW 3 cut(s) 158, 1207, 1289
TauI GCSGC 4 cut(s) 8, 62, 142, 649
TfiI GAWTC 6 cut(s) 75, 169, 578, 1418, 1607, 2008
TscAI CASTG 7 cut(s) 637, 1609, 1869, 1881, 1896, 2188, 2313
TseFI GTSAC 1 cut(s) 2308
TseI GCWGC 5 cut(s) 28, 422, 649, 1202, 1700
Tsp45I GTSAC 1 cut(s) 2308
TspDTI ATGAA 8 cut(s) 170, 182, 714, 996, 1583, 2040, 2154, 2340
TspGWI ACGGA 2 cut(s) 754, 2323
TspRI CASTG 7 cut(s) 637, 1609, 1869, 1881, 1896, 2188, 2313
Van91I CCANNNNNTGG 1 cut(s) 911
VpaK11BI GGWCC 3 cut(s) 70, 896, 1626
XapI RAATTY 6 cut(s) 50, 623, 836, 1090, 1495, 2027
XceI RCATGY 3 cut(s) 394, 1174, 1274
XhoI CTCGAG 1 cut(s) 2377
XmaJI CCTAGG 1 cut(s) 1616
XmiI GTMKAC 1 cut(s) 1922
XspI CTAG 2 cut(s) 935, 1617
ZrmI AGTACT 1 cut(s) 160
Zsp2I ATGCAT 3 cut(s) 1084, 1172, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.