RLG00000032445

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000525
Physical Location & Seq
Forward (+)
15346651 .. 15351341
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032445

Sequence Viewer

Length: 669 bp
ATGCCCTATCATCGCTTCTCATCCTCCTATCAGCGTTCTTCAGAGCCGCCGCCGATGATGATTCCCGGTCAGCCTACGACGTCATCTAAAAGCGTCACCGACTACGACCTCGACCTGAGCAACGATAATTGGCTGCAACTTTTCTCTCTAAGGCTCCTCGAGCTCGTCTTCAACCTCACCGAACTGTACCAGGACCTGATCGACACCCTCCAGAACCTCGACTTCGGCGGCAAGATGACACAGTTGTCTGGCGACTCGGGGAAGGGATGTGGTGGTGGTAGTGGCAGCTACGGTGGGAGCGGTCTGCGGGAACGGGAGAGGGAGCGTGAGCAGCTTAGTGCGCTGTCGCTTAAGCTTTTGTCAGTCATGGCTGCCGTCTGCTTGGATAAGAAGCCCAATAATATAGAGAAAGTTCACTTCTCTTCTCTAATGTCGTTGGATGGAACTGGAGCTCCTCTTGCCTCAACCAATTGGGATGAGGTTTCTGTAAGAGACACCTTAATTAAACCAGTGCAGTGCAAGCCCTTGTGGAAGCAGTTGAAAGAAGAGACTGCCGCAGAACAGACCATTGCAAATGGTTCTGAGCGTAACAGGCTGAGGCAAGAAACTGAGGTTTCAACTAGGAATAACCTGCCTCCATCAAACCAGAGGTTTGGACACAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

223

Amino Acids

24.71

Weight (kDa)

5.97

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sey1_3HB PF20428 115 - 188 3.6e-06 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 639
AccBSI CCGCTC 1 cut(s) 300
AciI CCGC 6 cut(s) 47, 50, 228, 300, 307, 555
AcuI CTGAAG 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 1 cut(s) 188
AflII CTTAAG 1 cut(s) 350
AgsI TTSAA 3 cut(s) 172, 541, 618
AjnI CCWGG 1 cut(s) 189
AloI GAACNNNNNNTCC 2 cut(s) 436, 468
AluBI AGCT 5 cut(s) 163, 288, 334, 355, 452
AluI AGCT 5 cut(s) 163, 288, 334, 355, 452
Alw21I GWGCWC 2 cut(s) 165, 454
Alw26I GTCTC 2 cut(s) 486, 542
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 2 cut(s) 158, 256
ApeKI GCWGC 4 cut(s) 133, 285, 331, 371
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 88, 169
AvaI CYCGRG 2 cut(s) 158, 256
AvaII GGWCC 1 cut(s) 193
BanII GRGCYC 2 cut(s) 165, 454
BbsI GAAGAC 1 cut(s) 160
Bbv12I GWGCWC 2 cut(s) 165, 454
BbvCI CCTCAGC 1 cut(s) 596
BbvI GCAGC 4 cut(s) 120, 297, 343, 358
BccI CCATC 2 cut(s) 434, 646
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 191
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 486, 542
BfaI CTAG 1 cut(s) 621
BfrI CTTAAG 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 639
BisI GCNGC 8 cut(s) 47, 50, 134, 229, 286, 332, 372, 555
BlsI GCNGC 8 cut(s) 48, 51, 135, 230, 287, 333, 373, 556
Bme1390I CCNGG 2 cut(s) 66, 191
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 2 cut(s) 158, 256
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 66, 191
BpiI GAAGAC 1 cut(s) 160
BpmI CTGGAG 2 cut(s) 194, 468
Bpu10I CCTNAGC 2 cut(s) 116, 596
BpuMI CCSGG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bse1I ACTGG 2 cut(s) 451, 509
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 191
BseGI GGATG 4 cut(s) 20, 272, 445, 481
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 4 cut(s) 107, 573, 587, 600
BseNI ACTGG 2 cut(s) 451, 509
BseRI GAGGAG 2 cut(s) 146, 444
BseXI GCAGC 4 cut(s) 120, 297, 343, 358
BsgI GTGCAG 1 cut(s) 533
BsiHKAI GWGCWC 2 cut(s) 165, 454
BsiHKCI CYCGRG 2 cut(s) 158, 256
BsiSI CCGG 1 cut(s) 66
BsmAI GTCTC 2 cut(s) 486, 542
BsoBI CYCGRG 2 cut(s) 158, 256
Bsp1286I GDGCHC 2 cut(s) 165, 454
Bsp143I GATC 1 cut(s) 198
BspACI CCGC 6 cut(s) 47, 50, 228, 300, 307, 555
BspCNI CTCAG 4 cut(s) 108, 574, 588, 601
BspLI GGNNCC 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 639
BspTI CTTAAG 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 2 cut(s) 451, 509
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 186, 243, 293
Bst6I CTCTTC 2 cut(s) 427, 540
BstACI GRCGYC 1 cut(s) 80
BstAFI CTTAAG 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 6 cut(s) 116, 149, 335, 582, 596, 609
BstF5I GGATG 4 cut(s) 20, 272, 445, 481
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 2 cut(s) 486, 542
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 6 cut(s) 160, 331, 340, 458, 520, 592
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 64, 189
BstV1I GCAGC 4 cut(s) 120, 297, 343, 358
BstV2I GAAGAC 1 cut(s) 160
BstXI CCANNNNNNTGG 1 cut(s) 653
BtsCI GGATG 4 cut(s) 20, 272, 445, 481
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 2 cut(s) 516, 521
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 1 cut(s) 521
CaiI CAGNNNCTG 1 cut(s) 196
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 187
CviAII CATG 1 cut(s) 367
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 6 cut(s) 116, 149, 335, 582, 596, 609
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 427, 540
EarI CTCTTC 2 cut(s) 427, 540
Ecl136II GAGCTC 2 cut(s) 163, 452
Eco24I GRGCYC 2 cut(s) 165, 454
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 2 cut(s) 163, 452
Eco57I CTGAAG 1 cut(s) 24
Eco88I CYCGRG 2 cut(s) 158, 256
EcoICRI GAGCTC 2 cut(s) 163, 452
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 189
EcoT38I GRGCYC 2 cut(s) 165, 454
FaeI CATG 1 cut(s) 370
FaiI YATR 2 cut(s) 368, 404
FatI CATG 1 cut(s) 366
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 8 cut(s) 47, 50, 134, 229, 286, 332, 372, 555
FokI GGATG 4 cut(s) 7, 279, 452, 488
FriOI GRGCYC 2 cut(s) 165, 454
Fsp4HI GCNGC 8 cut(s) 47, 50, 134, 229, 286, 332, 372, 555
FspBI CTAG 1 cut(s) 621
GlaI GCGC 1 cut(s) 342
GluI GCNGC 8 cut(s) 47, 50, 134, 229, 286, 332, 372, 555
GsuI CTGGAG 2 cut(s) 194, 468
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 2 cut(s) 61, 254
HpaII CCGG 1 cut(s) 66
HphI GGTGA 2 cut(s) 88, 169
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 2 cut(s) 43, 583
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 186, 243, 293
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 4 cut(s) 136, 514, 519, 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 160, 331, 340, 458, 520, 592
HpyF3I CTNAG 6 cut(s) 116, 149, 335, 582, 596, 609
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 5 cut(s) 159, 297, 322, 449, 457
Lsp1109I GCAGC 4 cut(s) 120, 297, 343, 358
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 94, 587
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 300
MboI GATC 1 cut(s) 198
MboII GAAGA 4 cut(s) 30, 160, 414, 557
MfeI CAATTG 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 165, 454
MluCI AATT 4 cut(s) 127, 469, 501, 664
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 417
MseI TTAA 3 cut(s) 351, 500, 504
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 2 cut(s) 66, 191
MunI CAATTG 1 cut(s) 469
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 6 cut(s) 160, 331, 340, 458, 520, 592
NciI CCSGG 1 cut(s) 66
NdeII GATC 1 cut(s) 198
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 94
PacI TTAATTAA 1 cut(s) 504
PaeR7I CTCGAG 1 cut(s) 158
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 8 cut(s) 48, 51, 135, 230, 287, 333, 373, 556
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PpuMI RGGWCCY 1 cut(s) 193
Psp124BI GAGCTC 2 cut(s) 165, 454
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
PspXI VCTCGAGB 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SacI GAGCTC 2 cut(s) 165, 454
SaqAI TTAA 3 cut(s) 351, 500, 504
SatI GCNGC 8 cut(s) 47, 50, 134, 229, 286, 332, 372, 555
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 66, 191
SduI GDGCHC 2 cut(s) 165, 454
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 158
SmlI CTYRAG 2 cut(s) 158, 350
SmoI CTYRAG 2 cut(s) 158, 350
Sse9I AATT 4 cut(s) 127, 469, 501, 664
SsiI CCGC 6 cut(s) 47, 50, 228, 300, 307, 555
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 2 cut(s) 165, 454
StyD4I CCNGG 2 cut(s) 64, 189
TaaI ACNGT 3 cut(s) 186, 243, 293
TaiI ACGT 1 cut(s) 83
TaqI TCGA 4 cut(s) 111, 159, 201, 219
TasI AATT 4 cut(s) 127, 469, 501, 664
TauI GCSGC 4 cut(s) 49, 52, 231, 557
TfiI GAWTC 1 cut(s) 61
Tru1I TTAA 3 cut(s) 351, 500, 504
Tru9I TTAA 3 cut(s) 351, 500, 504
TscAI CASTG 2 cut(s) 516, 521
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 4 cut(s) 133, 285, 331, 371
Tsp45I GTSAC 1 cut(s) 94
TspRI CASTG 2 cut(s) 516, 521
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 158
XspI CTAG 1 cut(s) 621
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.