MD05G1256900.v1.1

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000525
Physical Location & Seq
Forward (+)
38780849 .. 38787354
6506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1256900.v1.1.491

Sequence Viewer

Length: 2499 bp
ATGGACGAAGATTGCTGCGCCACGCAACTTATCTATGGCGATGGAGAATTTAACGCAGATGGGCTCGACAGGTTCGTGAAGGAGGTGAAGCTTACCGAGTGCGGACTCTCCTATGCGGTTGTTGCAATCATGGGTCCGCAGAGCAGCGGGAAAAGCACTTTGTTAAATCATTTGTTTCGGACTAAGTTTAGGGAGATGGATGCTTACAGTGGAAGGAGCCAAACTACAAAGGGTATTTGGATTGCCAAGTGTGTTGGCATTGAGCCGTGCACGATTGCCATGGATTTGGAGGGGACTGATGGGAGGGAGAGGGGCGAGGATGATACTACATTCGAGAAACAAAGCGCCCTATTTGCCTTGGCAGTTTCAGACATCGTGCTGATAAATATGTGGTGTCATGATATTGGTCGGGAGCAAGCTGCCAATAAACCTTTACTAAAAACAGTTTTTCAGGTCATGATGCGCCTATTCAGCCCCCGTAAAACAACGTTACTTTTTGTTATACGTGATAAAACAAAGACCCCTTTTGAATACCTGGAACCTGTGCTAAGGGAAGATATACAGAAGATATGGGATGGAGTACCGAAGCCTCAAGCCCATAAAAGCACCCCATTGAGTGAATTTTTTACTGTAGAAGTAGTTGCTTTGTCCAGTTACGAAGAGAAGGAGGAGAAGTTTAAGGAGGAGGTTGCTCAATTGAGGCAGCGCTTTTTCCATTCTATTTCTCCAGGAGGGCTTGCTGGCGATAGAAGGGGTGTTGTCCCTGCGTCAGGATTTTCTTTTAGTGCACAACAGATTTGGAAAGTAATCAAAGAGAACAAGGACTTGGATCTTCCCGCTCACAAGGTTATGGTTGCTACGGTTCGGTGTGAAGAGATTGCCAACCAGAAATTCAAACAGTTGGTCCATGATGAGGGTTGGTTAGCACTGCAAGAAGCTGTTGAAACTGGTCCTGTACAAGGCTTTGGTAAAAGGCTCAGTTCCATTCTAGCCACCTATCTTTCCGAGTATGACATGGAGGCCATCTACTTTGATGAAGGTGTAAGGAACTCAAAACGACAACTGTTGGAGTCAAAAGCATTGGATTTTGTTTACCCTGCGTACTCGGCCATGCTCGGACACCTACGTTCTAAAGCCCTTGAAGATTTTCAAGTGAGGCTGGAGCAGTCGTTGAACAAAGGAGAAGGATTCGCTTCATCTGTTCGTACCTGTGCTCAGTCTTCTATGCTTGAGTTTGAGAAAGGATGTGCAGATGCTGCCATACAACAAGCTAATTGGGATGCTTCAAAAGTCCGGGAAAAGCTTCAACGTGATATAGATGCACATGCATCATCGGTTCGTGGTGCAAAACTGGCAGAATTGAATTCCAACTATGAGAAAAAACTTTCTTCGTCTTTAAGCGGTCCTGTAGAGGCTCTACTGGAAACTGGTGCAAAAGACACCTGGGCTTCGATACGAAAACTACTTAATCGTGAGACCAAAGTTGCAGTATCGGAGTTCTCAACTGCAGTTGCCAATTTTGAGTTGGACAATGAAACGGTTGTCAAAATGAAGCAGCATTTGAAGGATTATGCGAGAAATGTGGTGGAGACAAAAGCAAGAGAAGAGGCTGGGAAAATTATGATCCACATGAAGGATCGGTTTGCCGCAGTCTTCAATTATGACAGCGATTCAATGCCTAGGGTTTGGACTGGGAACGAAGACATTAGAAGTATTACCAAGGATGCACGAACTGCGTCTCTGAAGCTTTTGTCGACCATGGCTGCCATTCGCTTGGATGAGAAGCCAGATAATATTGAAAATGTCCTCGTTTCTTCTCTGGTGGACGGGACTGTTACTGTTTCATCTTCACAAAATAGGAAACTAGGACCTTCTACAGATCCTCTTGCCTCAAGCTCTTGGGAAGAGGTTTCTTCAAAGGATACCTTAATTACCCCAGTACAGTGCAAGTCATTGTGGAGGCAGTTCAAAGCAGAGACGGAATATAGCGTCACTCAAGCTATTTCGGCACAGGAGGCTCACAAGCGGAGTAACAACTGGTTACCTCCTCCATGGGCTATAATGGCGATGATCGTTCTTGGTTTTAACGAATTTATGATGCTTTTAAAGAACCCTCTCTACCTCATGGTTCTATTTGTTGCATTTTTACTTTCAAAGGCGTTATGGGTACAGATGGACATTACGGGACAGTTCCAGCATGGCGCTCTGTCAGGAATACTGTCTATCTCATCAAGGTTTCTTCCAACCGTCATGAATCTTCTAAGAAAACTCGCAGAAGAAGCTCAGGGGAATCAAACACCGGAAGCACAAAGGCGGCCAGTTTCTCTTTCTTCTCAGAGTTATAGAAATGAAACACCTCAGCCAAATCCAGTAACAAGCTCATTCCCGGAGTCCTCAGTGTCATCCAATATCTCGTCATCAGATACCGGCATGGAATACTCAAGCCCTCCTTTAAGACAAAGGCGAACGGCAAACGTTGAGGAAGTTGAATCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

833

Amino Acids

93.05

Weight (kDa)

5.93

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
URGCP_GTPase PF25683 38 - 159 2.3e-06 URGCP-like GTPase domain
RHD3_GTPase PF05879 44 - 279 3.2e-114 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 321 - 760 9.4e-144 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 839
AccI GTMKAC 1 cut(s) 1754
AciI CCGC 9 cut(s) 102, 116, 137, 147, 837, 1401, 1647, 2026, 2314
AclI AACGTT 2 cut(s) 488, 2475
AclWI GGATC 4 cut(s) 837, 1618, 1644, 1874
AcoI YGGCCR 2 cut(s) 1107, 2315
AcsI RAATTY 5 cut(s) 47, 620, 890, 1363, 2090
AcuI CTGAAG 1 cut(s) 1763
AfaI GTAC 6 cut(s) 582, 957, 1103, 1207, 1941, 2169
AfeI AGCGCT 1 cut(s) 707
AfiI CCNNNNNNNGG 4 cut(s) 770, 913, 959, 1633
AjnI CCWGG 3 cut(s) 534, 727, 1442
AjuI GAANNNNNNNTTGG 4 cut(s) 809, 841, 875, 907
Alw21I GWGCWC 3 cut(s) 272, 790, 1216
Alw26I GTCTC 4 cut(s) 1469, 1583, 1743, 1970
Alw44I GTGCAC 2 cut(s) 268, 786
AlwI GGATC 4 cut(s) 837, 1618, 1644, 1874
AlwNI CAGNNNCTG 1 cut(s) 1256
Aor51HI AGCGCT 1 cut(s) 707
AoxI GGCC 3 cut(s) 1020, 1107, 2315
ApaLI GTGCAC 2 cut(s) 268, 786
ApeKI GCWGC 7 cut(s) 15, 144, 419, 703, 1256, 1555, 1763
ApoI RAATTY 5 cut(s) 47, 620, 890, 1363, 2090
ArsI GACNNNNNNTTYG 2 cut(s) 888, 920
Asp700I GAANNNNTTC 2 cut(s) 1146, 1302
AspA2I CCTAGG 1 cut(s) 1679
AspLEI GCGC 5 cut(s) 20, 347, 465, 708, 2204
AspS9I GGNCC 5 cut(s) 134, 904, 950, 1403, 1868
AsuC2I CCSGG 2 cut(s) 1295, 2387
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 5 cut(s) 134, 904, 950, 1403, 1868
AvrII CCTAGG 1 cut(s) 1679
BaeGI GKGCMC 2 cut(s) 272, 790
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 3 cut(s) 1212, 1645, 1707
Bbv12I GWGCWC 3 cut(s) 272, 790, 1216
BbvCI CCTCAGC 1 cut(s) 2358
BbvI GCAGC 7 cut(s) 2, 156, 406, 715, 1243, 1567, 1750
BccI CCATC 7 cut(s) 35, 53, 190, 293, 569, 1031, 2167
BceAI ACGGC 2 cut(s) 250, 2484
BciT130I CCWGG 3 cut(s) 536, 729, 1444
BciVI GTATCC 1 cut(s) 1915
BcnI CCSGG 2 cut(s) 1295, 2387
BcoDI GTCTC 4 cut(s) 1469, 1583, 1743, 1970
BfaI CTAG 3 cut(s) 989, 1680, 1865
BfmI CTRYAG 4 cut(s) 630, 1407, 1506, 1875
BfoI RGCGCY 3 cut(s) 348, 709, 2205
BfuI GTATCC 1 cut(s) 1915
BisI GCNGC 9 cut(s) 16, 145, 420, 704, 1257, 1556, 1647, 1764, 2315
BlnI CCTAGG 1 cut(s) 1679
BlsI GCNGC 9 cut(s) 17, 146, 421, 705, 1258, 1557, 1648, 1765, 2316
Bme1390I CCNGG 5 cut(s) 536, 729, 1295, 1444, 2387
Bme18I GGWCC 5 cut(s) 134, 904, 950, 1403, 1868
BmgT120I GGNCC 5 cut(s) 134, 904, 950, 1403, 1868
BmiI GGNNCC 3 cut(s) 135, 218, 540
BmrFI CCNGG 5 cut(s) 536, 729, 1295, 1444, 2387
BmrI ACTGGG 2 cut(s) 1701, 1931
BmsI GCATC 8 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1714, 2088
BmuI ACTGGG 2 cut(s) 1701, 1931
BpiI GAAGAC 3 cut(s) 1212, 1645, 1707
BpmI CTGGAG 2 cut(s) 711, 1181
Bpu10I CCTNAGC 3 cut(s) 548, 2283, 2358
BpuEI CTTGAG 5 cut(s) 576, 1250, 1876, 1980, 2425
BpuMI CCSGG 2 cut(s) 1295, 2387
BsaAI YACGTR 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 1469
BsaJI CCNNGG 7 cut(s) 279, 357, 1443, 1679, 1719, 1758, 2051
BsaWI WCCGGW 1 cut(s) 2299
BsaXI ACNNNNNCTCC 4 cut(s) 659, 689, 1154, 1184
Bsc4I CCNNNNNNNGG 4 cut(s) 770, 913, 959, 1633
Bse118I RCCGGY 1 cut(s) 2426
BseBI CCWGG 3 cut(s) 536, 729, 1444
BseDI CCNNGG 7 cut(s) 279, 357, 1443, 1679, 1719, 1758, 2051
BseGI GGATG 8 cut(s) 205, 325, 580, 1250, 1285, 1729, 1783, 2402
BseLI CCNNNNNNNGG 4 cut(s) 770, 913, 959, 1633
BseMII CTCAG 6 cut(s) 991, 1229, 2297, 2348, 2372, 2409
BseRI GAGGAG 3 cut(s) 683, 698, 2037
BseSI GKGCMC 2 cut(s) 272, 790
BseXI GCAGC 7 cut(s) 2, 156, 406, 715, 1243, 1567, 1750
BseYI CCCAGC 1 cut(s) 1610
BsgI GTGCAG 1 cut(s) 1269
BshFI GGCC 3 cut(s) 1022, 1109, 2317
BsiHKAI GWGCWC 3 cut(s) 272, 790, 1216
BsiSI CCGG 4 cut(s) 1294, 2300, 2387, 2427
BslFI GGGAC 4 cut(s) 307, 746, 1843, 2199
BslI CCNNNNNNNGG 4 cut(s) 770, 913, 959, 1633
BsmAI GTCTC 4 cut(s) 1469, 1583, 1743, 1970
BsmBI CGTCTC 2 cut(s) 1743, 1970
BsmFI GGGAC 4 cut(s) 307, 746, 1843, 2199
BsnI GGCC 3 cut(s) 1022, 1109, 2317
Bso31I GGTCTC 1 cut(s) 1469
Bsp1286I GDGCHC 4 cut(s) 66, 272, 790, 1216
Bsp1407I TGTACA 1 cut(s) 955
Bsp143I GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
Bsp19I CCATGG 3 cut(s) 279, 1758, 2051
BspACI CCGC 9 cut(s) 102, 116, 137, 147, 837, 1401, 1647, 2026, 2314
BspANI GGCC 3 cut(s) 1022, 1109, 2317
BspCNI CTCAG 6 cut(s) 990, 1228, 2296, 2347, 2371, 2408
BspHI TCATGA 4 cut(s) 397, 456, 2250, 2495
BspLI GGNNCC 3 cut(s) 135, 218, 540
BspMAI CTGCAG 1 cut(s) 1510
BspPI GGATC 4 cut(s) 837, 1618, 1644, 1874
BspTNI GGTCTC 1 cut(s) 1469
BsrBI CCGCTC 1 cut(s) 839
BsrFI RCCGGY 1 cut(s) 2426
BsrGI TGTACA 1 cut(s) 955
BssAI RCCGGY 1 cut(s) 2426
BssECI CCNNGG 7 cut(s) 279, 357, 1443, 1679, 1719, 1758, 2051
BssMI GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
BssT1I CCWWGG 6 cut(s) 279, 357, 1679, 1719, 1758, 2051
Bst2UI CCWGG 3 cut(s) 536, 729, 1444
Bst6I CTCTTC 4 cut(s) 654, 867, 1599, 1899
BstAPI GCANNNNNTGC 2 cut(s) 1256, 1733
BstAUI TGTACA 1 cut(s) 955
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 417, 738, 742
BstDEI CTNAG 9 cut(s) 183, 548, 977, 1215, 2261, 2283, 2334, 2358, 2395
BstDSI CCRYGG 3 cut(s) 279, 1758, 2051
BstEII GGTNACC 1 cut(s) 2040
BstENI CCTNNNNNAGG 2 cut(s) 768, 957
BstF5I GGATG 8 cut(s) 205, 325, 580, 1250, 1285, 1729, 1783, 2402
BstH2I RGCGCY 3 cut(s) 348, 709, 2205
BstHHI GCGC 5 cut(s) 20, 347, 465, 708, 2204
BstKTI GATC 5 cut(s) 832, 1626, 1639, 1882, 2073
BstMAI GTCTC 4 cut(s) 1469, 1583, 1743, 1970
BstMBI GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
BstNI CCWGG 3 cut(s) 536, 729, 1444
BstNSI RCATGY 1 cut(s) 1328
BstPI GGTNACC 1 cut(s) 2040
BstSCI CCNGG 5 cut(s) 534, 727, 1293, 1442, 2385
BstSFI CTRYAG 4 cut(s) 630, 1407, 1506, 1875
BstSLI GKGCMC 2 cut(s) 272, 790
BstV1I GCAGC 7 cut(s) 2, 156, 406, 715, 1243, 1567, 1750
BstV2I GAAGAC 3 cut(s) 1212, 1645, 1707
BstX2I RGATCY 2 cut(s) 829, 1879
BstXI CCANNNNNNTGG 2 cut(s) 286, 1774
BstYI RGATCY 2 cut(s) 829, 1879
BsuI GTATCC 1 cut(s) 1915
BsuRI GGCC 3 cut(s) 1022, 1109, 2317
BtgI CCRYGG 3 cut(s) 279, 1758, 2051
BtgZI GCGATG 2 cut(s) 54, 2081
BtsCI GGATG 8 cut(s) 205, 325, 580, 1250, 1285, 1729, 1783, 2402
BtsI GCAGTG 1 cut(s) 926
BtsIMutI CAGTG 4 cut(s) 214, 926, 1949, 2403
Cac8I GCNNGC 3 cut(s) 417, 738, 742
CaiI CAGNNNCTG 1 cut(s) 1256
CciI TCATGA 4 cut(s) 397, 456, 2250, 2495
CfoI GCGC 5 cut(s) 20, 347, 465, 708, 2204
Cfr10I RCCGGY 1 cut(s) 2426
Cfr13I GGNCC 5 cut(s) 134, 904, 950, 1403, 1868
CseI GACGC 3 cut(s) 756, 1725, 1978
Csp6I GTAC 6 cut(s) 581, 956, 1102, 1206, 1940, 2168
CviQI GTAC 6 cut(s) 581, 956, 1102, 1206, 1940, 2168
DdeI CTNAG 9 cut(s) 183, 548, 977, 1215, 2261, 2283, 2334, 2358, 2395
DpnI GATC 5 cut(s) 831, 1625, 1638, 1881, 2072
DpnII GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
DraI TTTAAA 1 cut(s) 2106
EaeI YGGCCR 2 cut(s) 1107, 2315
Eam1104I CTCTTC 4 cut(s) 654, 867, 1599, 1899
EarI CTCTTC 4 cut(s) 654, 867, 1599, 1899
Eco130I CCWWGG 6 cut(s) 279, 357, 1679, 1719, 1758, 2051
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 1469
Eco47I GGWCC 5 cut(s) 134, 904, 950, 1403, 1868
Eco47III AGCGCT 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 1763
Eco91I GGTNACC 1 cut(s) 2040
EcoNI CCTNNNNNAGG 2 cut(s) 768, 957
EcoO109I RGGNCCY 1 cut(s) 1868
EcoO65I GGTNACC 1 cut(s) 2040
EcoRI GAATTC 1 cut(s) 1363
EcoRII CCWGG 3 cut(s) 534, 727, 1442
EcoT14I CCWWGG 6 cut(s) 279, 357, 1679, 1719, 1758, 2051
EcoT22I ATGCAT 1 cut(s) 1330
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 6 cut(s) 279, 357, 1679, 1719, 1758, 2051
Esp3I CGTCTC 2 cut(s) 1743, 1970
FalI AAGNNNNNCTT 4 cut(s) 1910, 1942, 2434, 2466
FaqI GGGAC 4 cut(s) 307, 746, 1843, 2199
FauI CCCGC 2 cut(s) 140, 844
FblI GTMKAC 1 cut(s) 1754
Fnu4HI GCNGC 9 cut(s) 16, 145, 420, 704, 1257, 1556, 1647, 1764, 2315
FokI GGATG 8 cut(s) 212, 332, 587, 1257, 1292, 1736, 1790, 2389
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 9 cut(s) 16, 145, 420, 704, 1257, 1556, 1647, 1764, 2315
FspBI CTAG 3 cut(s) 989, 1680, 1865
GlaI GCGC 5 cut(s) 19, 346, 464, 707, 2203
GluI GCNGC 9 cut(s) 16, 145, 420, 704, 1257, 1556, 1647, 1764, 2315
GsaI CCCAGC 1 cut(s) 1614
GsuI CTGGAG 2 cut(s) 711, 1181
HaeII RGCGCY 3 cut(s) 348, 709, 2205
HaeIII GGCC 3 cut(s) 1022, 1109, 2317
HapII CCGG 4 cut(s) 1294, 2300, 2387, 2427
HgaI GACGC 3 cut(s) 756, 1725, 1978
HhaI GCGC 5 cut(s) 20, 347, 465, 708, 2204
Hin6I GCGC 5 cut(s) 18, 345, 463, 706, 2202
HinP1I GCGC 5 cut(s) 18, 345, 463, 706, 2202
HincII GTYRAC 1 cut(s) 1755
HindII GTYRAC 1 cut(s) 1755
HindIII AAGCTT 3 cut(s) 89, 1301, 1745
HinfI GANTC 8 cut(s) 105, 1070, 1188, 1670, 2254, 2290, 2390, 2489
HpaII CCGG 4 cut(s) 1294, 2300, 2387, 2427
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 5 cut(s) 270, 788, 1093, 1755, 1825
Hpy188I TCNGA 8 cut(s) 180, 370, 1006, 1118, 1495, 1743, 2337, 2422
Hpy8I GTNNAC 5 cut(s) 270, 788, 1093, 1755, 1825
HpyAV CCTTC 9 cut(s) 73, 207, 658, 744, 1031, 1178, 1558, 1627, 1881
HpyCH4IV ACGT 5 cut(s) 488, 505, 1126, 1309, 2475
HpyF3I CTNAG 9 cut(s) 183, 548, 977, 1215, 2261, 2283, 2334, 2358, 2395
HpySE526I ACGT 5 cut(s) 488, 505, 1126, 1309, 2475
HspAI GCGC 5 cut(s) 18, 345, 463, 706, 2202
Kzo9I GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
LmnI GCTCC 3 cut(s) 216, 412, 1162
Lsp1109I GCAGC 7 cut(s) 2, 156, 406, 715, 1243, 1567, 1750
LweI GCATC 8 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1714, 2088
MaeI CTAG 3 cut(s) 989, 1680, 1865
MaeII ACGT 5 cut(s) 488, 505, 1126, 1309, 2475
MaeIII GTNAC 7 cut(s) 489, 653, 1834, 1990, 2030, 2040, 2371
MalI GATC 5 cut(s) 831, 1625, 1638, 1881, 2072
MbiI CCGCTC 1 cut(s) 839
MboI GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
MfeI CAATTG 1 cut(s) 695
MflI RGATCY 2 cut(s) 829, 1879
MhlI GDGCHC 4 cut(s) 66, 272, 790, 1216
MlyI GAGTC 3 cut(s) 99, 1079, 2399
MmeI TCCRAC 4 cut(s) 1047, 1392, 1506, 2267
Mph1103I ATGCAT 1 cut(s) 1330
MroXI GAANNNNTTC 2 cut(s) 1146, 1302
MseI TTAA 9 cut(s) 51, 164, 678, 1397, 1467, 1928, 2085, 2105, 2453
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 4 cut(s) 1294, 2300, 2387, 2427
MspR9I CCNGG 5 cut(s) 536, 729, 1295, 1444, 2387
MunI CAATTG 1 cut(s) 695
MvaI CCWGG 3 cut(s) 536, 729, 1444
NciI CCSGG 2 cut(s) 1295, 2387
NcoI CCATGG 3 cut(s) 279, 1758, 2051
NdeII GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
NlaIV GGNNCC 3 cut(s) 135, 218, 540
NmeAIII GCCGAG 1 cut(s) 1085
NmuCI GTSAC 1 cut(s) 1990
NsiI ATGCAT 1 cut(s) 1330
NspI RCATGY 1 cut(s) 1328
PagI TCATGA 4 cut(s) 397, 456, 2250, 2495
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 1146, 1302
PfeI GAWTC 5 cut(s) 1188, 1670, 2254, 2290, 2489
PfoI TCCNGGA 3 cut(s) 727, 1293, 2385
PkrI GCNGC 9 cut(s) 17, 146, 421, 705, 1258, 1557, 1648, 1765, 2316
PleI GAGTC 3 cut(s) 99, 1078, 2398
PpsI GAGTC 3 cut(s) 99, 1078, 2398
Ppu21I YACGTR 1 cut(s) 506
PpuMI RGGWCCY 1 cut(s) 1868
Psp1406I AACGTT 2 cut(s) 488, 2475
Psp5II RGGWCCY 1 cut(s) 1868
Psp6I CCWGG 3 cut(s) 534, 727, 1442
PspEI GGTNACC 1 cut(s) 2040
PspFI CCCAGC 1 cut(s) 1610
PspGI CCWGG 3 cut(s) 534, 727, 1442
PspN4I GGNNCC 3 cut(s) 135, 218, 540
PspPI GGNCC 5 cut(s) 134, 904, 950, 1403, 1868
PspPPI RGGWCCY 1 cut(s) 1868
PsrI GAACNNNNNNTAC 2 cut(s) 2102, 2134
PstI CTGCAG 1 cut(s) 1510
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 2 cut(s) 829, 1879
RsaI GTAC 6 cut(s) 582, 957, 1103, 1207, 1941, 2169
RsaNI GTAC 6 cut(s) 581, 956, 1102, 1206, 1940, 2168
SalI GTCGAC 1 cut(s) 1753
SaqAI TTAA 9 cut(s) 51, 164, 678, 1397, 1467, 1928, 2085, 2105, 2453
SatI GCNGC 9 cut(s) 16, 145, 420, 704, 1257, 1556, 1647, 1764, 2315
Sau3AI GATC 5 cut(s) 829, 1623, 1636, 1879, 2070
Sau96I GGNCC 5 cut(s) 134, 904, 950, 1403, 1868
SchI GAGTC 3 cut(s) 99, 1079, 2399
ScrFI CCNGG 5 cut(s) 536, 729, 1295, 1444, 2387
SduI GDGCHC 4 cut(s) 66, 272, 790, 1216
SfaNI GCATC 8 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1714, 2088
SfcI CTRYAG 4 cut(s) 630, 1407, 1506, 1875
SinI GGWCC 5 cut(s) 134, 904, 950, 1403, 1868
SmlI CTYRAG 5 cut(s) 591, 1229, 1891, 1995, 2440
SmoI CTYRAG 5 cut(s) 591, 1229, 1891, 1995, 2440
SsiI CCGC 9 cut(s) 102, 116, 137, 147, 837, 1401, 1647, 2026, 2314
SspI AATATT 1 cut(s) 1795
SspMI CTAG 3 cut(s) 989, 1680, 1865
StyD4I CCNGG 5 cut(s) 534, 727, 1293, 1442, 2385
StyI CCWWGG 6 cut(s) 279, 357, 1679, 1719, 1758, 2051
TaiI ACGT 5 cut(s) 491, 508, 1129, 1312, 2478
TaqI TCGA 4 cut(s) 66, 333, 1451, 1754
TatI WGTACW 2 cut(s) 955, 1939
TauI GCSGC 2 cut(s) 1649, 2317
TfiI GAWTC 5 cut(s) 1188, 1670, 2254, 2290, 2489
Tru1I TTAA 9 cut(s) 51, 164, 678, 1397, 1467, 1928, 2085, 2105, 2453
Tru9I TTAA 9 cut(s) 51, 164, 678, 1397, 1467, 1928, 2085, 2105, 2453
TscAI CASTG 4 cut(s) 214, 933, 1949, 2403
TseFI GTSAC 1 cut(s) 1990
TseI GCWGC 7 cut(s) 15, 144, 419, 703, 1256, 1555, 1763
Tsp45I GTSAC 1 cut(s) 1990
TspDTI ATGAA 9 cut(s) 1050, 1185, 1548, 1565, 1646, 1833, 2267, 2364, 2484
TspGWI ACGGA 1 cut(s) 1994
TspRI CASTG 4 cut(s) 214, 933, 1949, 2403
VneI GTGCAC 2 cut(s) 268, 786
VpaK11BI GGWCC 5 cut(s) 134, 904, 950, 1403, 1868
XagI CCTNNNNNAGG 2 cut(s) 768, 957
XapI RAATTY 5 cut(s) 47, 620, 890, 1363, 2090
XceI RCATGY 1 cut(s) 1328
XcmI CCANNNNNNNNNTGG 1 cut(s) 1522
XmaJI CCTAGG 1 cut(s) 1679
XmiI GTMKAC 1 cut(s) 1754
XmnI GAANNNNTTC 2 cut(s) 1146, 1302
XspI CTAG 3 cut(s) 989, 1680, 1865
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.