MD05G1257400.v1.1

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000525
Physical Location & Seq
Forward (+)
39366038 .. 39376168
10131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1257400.v1.1.491

Sequence Viewer

Length: 2781 bp
ATGGCTCACCTGGCGAGCCACATCCAAAACTACGAGGTCCGATTCCTCCCCGAGTTCTTTGACTCTCGCTCAAATTGGACGGATTTTCGAGTGGAACTGTTCGCCATGGCCTGCAGCAACGCCGCGGCCGAGTTGCTTGGGGAGGCTCTATGGGTTTGTACTGTTTCTAGTTCTCACTTTATTTTGTATGTCTTGATCTCGCTTATGCATCATAGGTATGACATGGAGACCATCTACTTTGATGAAGTTGTAGGGAACTCAAAACGACAACTGTTGGAGTCAAAAGCATTGGATTTTGTTTACCCTGCGTACTCGGCCATGCTCGGACACCTGCGATCTAAAGCCCTTGAAGATTTTCAAGTTAGGCTGGAGCAGTCATTGAACAAAGGAGAAGGATTCGCTTCATCTGTCATACTTGTGCTCAATGCTGCCATACAACAAGCTAATTGGGATGCTTCAAAAGTCCGGGAAAAGCTTCGACGTGATATAGATGCACATGCATCATCGGTTCGTAGTGCAAAACTGGCAGAATTGAATTCCAACTATGAGAAAAAACTTTCTTCAATACCAAAACTACTTAATCGTGAGACTGAAGTTGCAGCATCAGAGTTCTCAACTGCAGTTGCCAATTTTGAGTTGGACAACGAAACGGTTGCCAAAATGAAGCAACATTTGAAGGATTATGCGAGAAATGTGGTGGAGACAAAAGCAAGAGAAGAGGCTGGGAAAATTATGATCCACATGAAGGATCGGTTTGCCAGAGTCTTCAATTATGACAGCGATTCAATGCCTAGGGTTTGGACTGGGAACGAAGACATTAGAAGTATTACCAAGGATGCACGAACTGATTCACTGGGAACTTTTGTGGAATCTCTGAAGCTTTTGTCGACCATGGCTGCCTTTCGTTTGGATGAGAAGCCAGGTAATATTGAAAATGTCCTTGTTTCTTCTCTGGTGGACGGGACTGTTACTGTTTCATCTTCACAAAATAGGAAACTAGGACCTTCTACTGATCCTCTTGCCTCAAGCTCTTGGGAAGAGGTTTCTTCAAAGGATACCTTAATTACCCCAGTACAGTGCAAGTCATTGTGGAGGCTGAGATGGACGCATCACAACAGAGAAAGAAAGAGAGATGGCTTTTTCCATTCTATTTCTCCAGGAGGGCTTGCTGGCAATAGAAGGGGTGTTGTCCCTGCGTCAGGATTTTCCTTTAGTGCACAACAGATTTGGAAAGTAATCAAAGAGAACAAGGACTTGGATCTTCCCGCTCACAAGGTTATGGTTGCTACTGTTCGGTGTGAAGAGATTGCCAACCACAAATTCAAACAGTTGGTCCACAATGAGGGTTGGTTGGCACTGCAAGAAGCTGTTGAAACTGGTCCCGTACAAGGCTTTGGTAAAAGGCTCAGTTCCATTCTAGCCACCTATCTTTCCTATTCTGACTTTATTTTGTATGTCTTGATCTCACTTATGCATCATAGGTATGACATGGAGGCCATCTACTTTCATGAAGGTGTAAGGAACTCAAAACGACAACTGTTGGAGTCAAAAGCATTGAATTTTGTTTACCCTGCGTACTTGGCCATGCTTGGGCACCTACGTTCTAAAGCCCTTGAAGATTTTCAAGTGAGGCTGGAGCAGTCGTTGAACAAAGGAGAAGGATTCGCTTCATCTGTTCGTACCTGTGTTCAGTCTTCTATGCTTGAGTTTGAGAAAGGATGTGCCGATGCTGCCATACAACAAGCTAATTGGGATGCTTCAAAAGTCCGGGAAAAGCTTCGACGTGATATAGATGCACATGCATCATTGGTTCGTAGTGCAAAACTGGCAGAATTGAATTCCAACTATGAGAAAAAACTTTCTTCGTCTTTAAGCGGTCCTGTAGAGGCTCTACTGGAAACTGGTGCAAAAGACACCTCGGCTTCAATACGAAAACTACTTAATCGTGGGACCAAAGTTGCAGTATCGGAGTTCTCAACTGCAGTTGCCAATTTTGAGTTGGACAATGAAACGGTTGTCAAAATGAAGCAGCATTTGAAGGATTATGCGAGAAATGTGGTGGAGACAAAAGCAAGAGAAGTGGCTGGGAAAATTATGATCCACATGAAGGATCGGTATGCCGCAGTCTTCAATTATGACAGCGATTCAATGCCTAGGGTTTGGACTGGGAACGAAGACATTACAAGTATTGCCAAGGATGCACAAACTGCGGATGAGAAGCCAGATAATATTGAAAATGTCCTAGTTTCTTCTCTGGTGGACGGGACTGTTACTGTTTCATCTTCACAAAATAGGAAACTAGGACCTTCTATAGATCCTCTTGCCTCAAGCTCTTGGGAAGAGGTTTCTTCAAAGGATACCTTAATTACCCCAGTACAGTGCAAGTCATTGTGGGGGCAGTTCAAAGCAGAGACCGAATATAGCGTCACTCAAGCTATTTCGGCACAGGAGGCTTACAAGCGGAGTAACAACTGGTTACCTCCTCCATGGGCTATAATGGTGATGATCGTTCTTGGTTTTAACGAATTTATGATGCTTTTAAAGAACCCTCTCTACCTCATGGTTCTATTTGTTGCATTTTTACTTTCAAAGGCCTTATGGGTACAGATGGACATTACGGGACAGTTCCAGCATGGCGCTCTGTCCGGAATACTGTCTATCTCATCAAGGTTTCTTCCAACCGTCATGAATCTTCTAAGAAAACTCGCAGAAGAAGCTCAAGGGGAATCAAACACCGGAAGCACAAAGGCGGCCAGTTTCTCTTTCTTCTCAGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

927

Amino Acids

103.87

Weight (kDa)

7.11

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sey1_3HB PF20428 71 - 188 1.7e-23 Sey1 three-helix bundle domain
Sey1_3HB PF20428 188 - 365 2.2e-44 Sey1 three-helix bundle domain
RHD3_GTPase PF05879 375 - 422 1.3e-11 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 493 - 905 5.3e-117 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 339
Acc36I ACCTGC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 1593
AccBSI CCGCTC 1 cut(s) 1268
AccI GTMKAC 1 cut(s) 887
AccII CGCG 1 cut(s) 125
AccIII TCCGGA 1 cut(s) 2645
AciI CCGC 8 cut(s) 123, 125, 1266, 1875, 2121, 2210, 2461, 2750
AclWI GGATC 7 cut(s) 730, 756, 1007, 1266, 2092, 2118, 2309
AcoI YGGCCR 4 cut(s) 126, 315, 1581, 2751
AcsI RAATTY 5 cut(s) 535, 1319, 1558, 1837, 2525
AcuI CTGAAG 2 cut(s) 612, 896
AfaI GTAC 8 cut(s) 160, 311, 1074, 1386, 1577, 1681, 2376, 2604
AfiI CCNNNNNNNGG 6 cut(s) 745, 1199, 1342, 1388, 1590, 2107
AjiI CACGTC 2 cut(s) 482, 1784
AjnI CCWGG 3 cut(s) 9, 919, 1156
AjuI GAANNNNNNNTTGG 4 cut(s) 1238, 1270, 1304, 1336
Alw21I GWGCWC 2 cut(s) 423, 1219
Alw26I GTCTC 5 cut(s) 221, 581, 695, 2057, 2405
Alw44I GTGCAC 1 cut(s) 1215
AlwI GGATC 7 cut(s) 730, 756, 1007, 1266, 2092, 2118, 2309
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 2645
AoxI GGCC 7 cut(s) 108, 126, 315, 1494, 1581, 2592, 2751
ApaLI GTGCAC 1 cut(s) 1215
ApeKI GCWGC 6 cut(s) 114, 428, 599, 896, 1730, 2029
ApoI RAATTY 5 cut(s) 535, 1319, 1558, 1837, 2525
ArsI GACNNNNNNTTYG 4 cut(s) 1317, 1349, 2409, 2441
Asp700I GAANNNNTTC 5 cut(s) 354, 474, 847, 1620, 1776
AspA2I CCTAGG 2 cut(s) 791, 2153
AspLEI GCGC 1 cut(s) 2639
AspS9I GGNCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
AsuC2I CCSGG 2 cut(s) 467, 1769
AsuHPI GGTGA 1 cut(s) 2512
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
AvrII CCTAGG 2 cut(s) 791, 2153
BaeGI GKGCMC 2 cut(s) 1219, 1596
BalI TGGCCA 1 cut(s) 1583
BanI GGYRCC 1 cut(s) 1593
BbsI GAAGAC 5 cut(s) 757, 819, 1686, 2119, 2181
Bbv12I GWGCWC 2 cut(s) 423, 1219
BbvI GCAGC 6 cut(s) 126, 415, 611, 883, 1717, 2041
BccI CCATC 5 cut(s) 239, 1095, 1127, 1505, 2602
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 3 cut(s) 11, 921, 1158
BciVI GTATCC 2 cut(s) 1048, 2350
BcnI CCSGG 2 cut(s) 467, 1769
BcoDI GTCTC 5 cut(s) 221, 581, 695, 2057, 2405
BfaI CTAG 7 cut(s) 168, 792, 998, 1418, 2154, 2243, 2300
BfmI CTRYAG 5 cut(s) 112, 618, 1881, 1980, 2310
BfoI RGCGCY 1 cut(s) 2640
BfuAI ACCTGC 1 cut(s) 339
BfuI GTATCC 2 cut(s) 1048, 2350
BlnI CCTAGG 2 cut(s) 791, 2153
Bme1390I CCNGG 5 cut(s) 11, 467, 921, 1158, 1769
Bme18I GGWCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 2 cut(s) 482, 1784
BmgT120I GGNCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
BmiI GGNNCC 3 cut(s) 1381, 1595, 1951
BmrFI CCNGG 5 cut(s) 11, 467, 921, 1158, 1769
BmrI ACTGGG 5 cut(s) 813, 863, 1064, 2175, 2366
BmuI ACTGGG 5 cut(s) 813, 863, 1064, 2175, 2366
BpiI GAAGAC 5 cut(s) 757, 819, 1686, 2119, 2181
BpmI CTGGAG 3 cut(s) 389, 1140, 1655
BpuEI CTTGAG 5 cut(s) 1009, 1724, 2311, 2415, 2703
BpuMI CCSGG 2 cut(s) 467, 1769
BsaI GGTCTC 2 cut(s) 221, 2405
BsaJI CCNNGG 9 cut(s) 105, 123, 791, 831, 891, 1917, 2153, 2193, 2486
BsaWI WCCGGW 2 cut(s) 2645, 2735
BsaXI ACNNNNNCTCC 2 cut(s) 1628, 1658
Bsc4I CCNNNNNNNGG 6 cut(s) 745, 1199, 1342, 1388, 1590, 2107
BseAI TCCGGA 1 cut(s) 2645
BseBI CCWGG 3 cut(s) 11, 921, 1158
BseDI CCNNGG 9 cut(s) 105, 123, 791, 831, 891, 1917, 2153, 2193, 2486
BseGI GGATG 8 cut(s) 21, 457, 841, 916, 1724, 1759, 2203, 2218
BseLI CCNNNNNNNGG 6 cut(s) 745, 1199, 1342, 1388, 1590, 2107
BseMII CTCAG 2 cut(s) 1088, 1420
BseRI GAGGAG 1 cut(s) 2472
BseSI GKGCMC 2 cut(s) 1219, 1596
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 6 cut(s) 126, 415, 611, 883, 1717, 2041
BseYI CCCAGC 2 cut(s) 722, 2084
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 7 cut(s) 110, 128, 317, 1496, 1583, 2594, 2753
BshNI GGYRCC 1 cut(s) 1593
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 423, 1219
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 4 cut(s) 466, 1768, 2646, 2736
BslFI GGGAC 6 cut(s) 976, 1175, 1365, 1963, 2278, 2634
BslI CCNNNNNNNGG 6 cut(s) 745, 1199, 1342, 1388, 1590, 2107
BsmAI GTCTC 5 cut(s) 221, 581, 695, 2057, 2405
BsmFI GGGAC 6 cut(s) 976, 1175, 1365, 1963, 2278, 2634
BsnI GGCC 7 cut(s) 110, 128, 317, 1496, 1583, 2594, 2753
Bso31I GGTCTC 2 cut(s) 221, 2405
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 3 cut(s) 423, 1219, 1596
Bsp13I TCCGGA 1 cut(s) 2645
Bsp19I CCATGG 3 cut(s) 105, 891, 2486
BspACI CCGC 8 cut(s) 123, 125, 1266, 1875, 2121, 2210, 2461, 2750
BspANI GGCC 7 cut(s) 110, 128, 317, 1496, 1583, 2594, 2753
BspCNI CTCAG 2 cut(s) 1089, 1419
BspEI TCCGGA 1 cut(s) 2645
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 2 cut(s) 1507, 2685
BspLI GGNNCC 3 cut(s) 1381, 1595, 1951
BspMAI CTGCAG 3 cut(s) 116, 622, 1984
BspMI ACCTGC 1 cut(s) 339
BspPI GGATC 7 cut(s) 730, 756, 1007, 1266, 2092, 2118, 2309
BspT107I GGYRCC 1 cut(s) 1593
BspTNI GGTCTC 2 cut(s) 221, 2405
BsrBI CCGCTC 1 cut(s) 1268
BssECI CCNNGG 9 cut(s) 105, 123, 791, 831, 891, 1917, 2153, 2193, 2486
BssT1I CCWWGG 7 cut(s) 105, 791, 831, 891, 2153, 2193, 2486
Bst2UI CCWGG 3 cut(s) 11, 921, 1158
Bst6I CTCTTC 4 cut(s) 711, 1032, 1296, 2334
BstAPI GCANNNNNTGC 1 cut(s) 2207
BstC8I GCNNGC 4 cut(s) 16, 112, 1167, 1171
BstDEI CTNAG 4 cut(s) 1097, 1406, 2696, 2770
BstDSI CCRYGG 4 cut(s) 105, 123, 891, 2486
BstEII GGTNACC 1 cut(s) 2475
BstENI CCTNNNNNAGG 1 cut(s) 1197
BstF5I GGATG 8 cut(s) 21, 457, 841, 916, 1724, 1759, 2203, 2218
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 2640
BstHHI GCGC 1 cut(s) 2639
BstMAI GTCTC 5 cut(s) 221, 581, 695, 2057, 2405
BstMCI CGRYCG 1 cut(s) 129
BstNI CCWGG 3 cut(s) 11, 921, 1158
BstNSI RCATGY 2 cut(s) 500, 1802
BstPI GGTNACC 1 cut(s) 2475
BstSCI CCNGG 5 cut(s) 9, 465, 919, 1156, 1767
BstSFI CTRYAG 5 cut(s) 112, 618, 1881, 1980, 2310
BstSLI GKGCMC 2 cut(s) 1219, 1596
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 6 cut(s) 126, 415, 611, 883, 1717, 2041
BstV2I GAAGAC 5 cut(s) 757, 819, 1686, 2119, 2181
BstX2I RGATCY 2 cut(s) 1258, 2314
BstYI RGATCY 2 cut(s) 1258, 2314
BstZI CGGCCG 1 cut(s) 126
BsuI GTATCC 2 cut(s) 1048, 2350
BsuRI GGCC 7 cut(s) 110, 128, 317, 1496, 1583, 2594, 2753
BtgI CCRYGG 4 cut(s) 105, 123, 891, 2486
BtrI CACGTC 2 cut(s) 482, 1784
BtsCI GGATG 8 cut(s) 21, 457, 841, 916, 1724, 1759, 2203, 2218
BtsI GCAGTG 1 cut(s) 1355
BtsIMutI CAGTG 4 cut(s) 851, 1082, 1355, 2384
BveI ACCTGC 1 cut(s) 339
Cac8I GCNNGC 4 cut(s) 16, 112, 1167, 1171
CciI TCATGA 2 cut(s) 1507, 2685
CfoI GCGC 1 cut(s) 2639
Cfr13I GGNCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
Cfr42I CCGCGG 1 cut(s) 126
CseI GACGC 3 cut(s) 1114, 1185, 2413
Csp6I GTAC 8 cut(s) 159, 310, 1073, 1385, 1576, 1680, 2375, 2603
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
CviQI GTAC 8 cut(s) 159, 310, 1073, 1385, 1576, 1680, 2375, 2603
DdeI CTNAG 4 cut(s) 1097, 1406, 2696, 2770
DraI TTTAAA 1 cut(s) 2541
EaeI YGGCCR 4 cut(s) 126, 315, 1581, 2751
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 4 cut(s) 711, 1032, 1296, 2334
EarI CTCTTC 4 cut(s) 711, 1032, 1296, 2334
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 7 cut(s) 105, 791, 831, 891, 2153, 2193, 2486
Eco147I AGGCCT 1 cut(s) 2594
Eco31I GGTCTC 2 cut(s) 221, 2405
Eco47I GGWCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
Eco52I CGGCCG 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 612, 896
Eco88I CYCGRG 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 2475
EcoNI CCTNNNNNAGG 1 cut(s) 1197
EcoO109I RGGNCCY 2 cut(s) 1001, 2303
EcoO65I GGTNACC 1 cut(s) 2475
EcoRI GAATTC 2 cut(s) 535, 1837
EcoRII CCWGG 3 cut(s) 9, 919, 1156
EcoT14I CCWWGG 7 cut(s) 105, 791, 831, 891, 2153, 2193, 2486
EcoT22I ATGCAT 4 cut(s) 210, 502, 1476, 1804
ErhI CCWWGG 7 cut(s) 105, 791, 831, 891, 2153, 2193, 2486
FalI AAGNNNNNCTT 4 cut(s) 1043, 1075, 2345, 2377
FaqI GGGAC 6 cut(s) 976, 1175, 1365, 1963, 2278, 2634
FauI CCCGC 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 887
FokI GGATG 8 cut(s) 8, 464, 848, 923, 1731, 1766, 2210, 2225
FspBI CTAG 7 cut(s) 168, 792, 998, 1418, 2154, 2243, 2300
GlaI GCGC 1 cut(s) 2638
GsaI CCCAGC 2 cut(s) 726, 2088
GsuI CTGGAG 3 cut(s) 389, 1140, 1655
HaeII RGCGCY 1 cut(s) 2640
HaeIII GGCC 7 cut(s) 110, 128, 317, 1496, 1583, 2594, 2753
HapII CCGG 4 cut(s) 466, 1768, 2646, 2736
HgaI GACGC 3 cut(s) 1114, 1185, 2413
HhaI GCGC 1 cut(s) 2639
Hin6I GCGC 1 cut(s) 2637
HinP1I GCGC 1 cut(s) 2637
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HindIII AAGCTT 3 cut(s) 473, 878, 1775
HpaII CCGG 4 cut(s) 466, 1768, 2646, 2736
HphI GGTGA 1 cut(s) 2512
Hpy166II GTNNAC 7 cut(s) 301, 888, 958, 1217, 1336, 1567, 2260
Hpy188I TCNGA 7 cut(s) 41, 326, 607, 876, 1441, 1969, 2773
Hpy188III TCNNGA 7 cut(s) 193, 584, 1200, 1459, 1508, 2646, 2686
Hpy8I GTNNAC 7 cut(s) 301, 888, 958, 1217, 1336, 1567, 2260
Hpy99I CGWCG 2 cut(s) 483, 1785
HpyCH4IV ACGT 3 cut(s) 481, 1600, 1783
HpyF3I CTNAG 4 cut(s) 1097, 1406, 2696, 2770
HpySE526I ACGT 3 cut(s) 481, 1600, 1783
HspAI GCGC 1 cut(s) 2637
Kpn2I TCCGGA 1 cut(s) 2645
KspI CCGCGG 1 cut(s) 126
LmnI GCTCC 2 cut(s) 370, 1636
Lsp1109I GCAGC 6 cut(s) 126, 415, 611, 883, 1717, 2041
MaeI CTAG 7 cut(s) 168, 792, 998, 1418, 2154, 2243, 2300
MaeII ACGT 3 cut(s) 481, 1600, 1783
MaeIII GTNAC 5 cut(s) 967, 2269, 2425, 2465, 2475
MbiI CCGCTC 1 cut(s) 1268
MflI RGATCY 2 cut(s) 1258, 2314
MhlI GDGCHC 3 cut(s) 423, 1219, 1596
MlsI TGGCCA 1 cut(s) 1583
MluNI TGGCCA 1 cut(s) 1583
MlyI GAGTC 4 cut(s) 56, 287, 771, 1553
MmeI TCCRAC 7 cut(s) 255, 564, 618, 1521, 1866, 1980, 2702
Mox20I TGGCCA 1 cut(s) 1583
Mph1103I ATGCAT 4 cut(s) 210, 502, 1476, 1804
MroI TCCGGA 1 cut(s) 2645
MroXI GAANNNNTTC 5 cut(s) 354, 474, 847, 1620, 1776
MscI TGGCCA 1 cut(s) 1583
MseI TTAA 7 cut(s) 579, 1061, 1871, 1941, 2363, 2520, 2540
MslI CAYNNNNRTG 4 cut(s) 216, 416, 1482, 1512
Msp20I TGGCCA 1 cut(s) 1583
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 4 cut(s) 466, 1768, 2646, 2736
MspR9I CCNGG 5 cut(s) 11, 467, 921, 1158, 1769
MvaI CCWGG 3 cut(s) 11, 921, 1158
MvnI CGCG 1 cut(s) 125
NciI CCSGG 2 cut(s) 467, 1769
NcoI CCATGG 3 cut(s) 105, 891, 2486
NlaIV GGNNCC 3 cut(s) 1381, 1595, 1951
NmeAIII GCCGAG 3 cut(s) 154, 293, 1898
NmuCI GTSAC 1 cut(s) 2425
NsiI ATGCAT 4 cut(s) 210, 502, 1476, 1804
NspI RCATGY 2 cut(s) 500, 1802
PagI TCATGA 2 cut(s) 1507, 2685
PaqCI CACCTGC 1 cut(s) 339
PceI AGGCCT 1 cut(s) 2594
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 5 cut(s) 354, 474, 847, 1620, 1776
PfeI GAWTC 9 cut(s) 42, 396, 782, 848, 869, 1662, 2144, 2689, 2726
PfoI TCCNGGA 3 cut(s) 465, 1156, 1767
PleI GAGTC 4 cut(s) 56, 286, 770, 1552
PpsI GAGTC 4 cut(s) 56, 286, 770, 1552
PpuMI RGGWCCY 2 cut(s) 1001, 2303
Psp5II RGGWCCY 2 cut(s) 1001, 2303
Psp6I CCWGG 3 cut(s) 9, 919, 1156
PspEI GGTNACC 1 cut(s) 2475
PspFI CCCAGC 2 cut(s) 722, 2084
PspGI CCWGG 3 cut(s) 9, 919, 1156
PspN4I GGNNCC 3 cut(s) 1381, 1595, 1951
PspPI GGNCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
PspPPI RGGWCCY 2 cut(s) 1001, 2303
PsrI GAACNNNNNNTAC 2 cut(s) 2537, 2569
PstI CTGCAG 3 cut(s) 116, 622, 1984
PsuI RGATCY 2 cut(s) 1258, 2314
RsaI GTAC 8 cut(s) 160, 311, 1074, 1386, 1577, 1681, 2376, 2604
RsaNI GTAC 8 cut(s) 159, 310, 1073, 1385, 1576, 1680, 2375, 2603
RseI CAYNNNNRTG 4 cut(s) 216, 416, 1482, 1512
SacII CCGCGG 1 cut(s) 126
SalI GTCGAC 1 cut(s) 886
SaqAI TTAA 7 cut(s) 579, 1061, 1871, 1941, 2363, 2520, 2540
Sau96I GGNCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
SchI GAGTC 4 cut(s) 56, 287, 771, 1553
ScrFI CCNGG 5 cut(s) 11, 467, 921, 1158, 1769
SduI GDGCHC 3 cut(s) 423, 1219, 1596
SfcI CTRYAG 5 cut(s) 112, 618, 1881, 1980, 2310
Sfr303I CCGCGG 1 cut(s) 126
SgrBI CCGCGG 1 cut(s) 126
SinI GGWCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
SmiMI CAYNNNNRTG 4 cut(s) 216, 416, 1482, 1512
SmlI CTYRAG 5 cut(s) 1024, 1703, 2326, 2430, 2718
SmoI CTYRAG 5 cut(s) 1024, 1703, 2326, 2430, 2718
SseBI AGGCCT 1 cut(s) 2594
SsiI CCGC 8 cut(s) 123, 125, 1266, 1875, 2121, 2210, 2461, 2750
SspI AATATT 2 cut(s) 928, 2230
SspMI CTAG 7 cut(s) 168, 792, 998, 1418, 2154, 2243, 2300
StuI AGGCCT 1 cut(s) 2594
StyD4I CCNGG 5 cut(s) 9, 465, 919, 1156, 1767
StyI CCWWGG 7 cut(s) 105, 791, 831, 891, 2153, 2193, 2486
TaiI ACGT 3 cut(s) 484, 1603, 1786
TaqI TCGA 4 cut(s) 88, 478, 887, 1780
TaqII GACCGA 1 cut(s) 2429
TatI WGTACW 3 cut(s) 158, 1072, 2374
TauI GCSGC 4 cut(s) 125, 128, 2123, 2753
TfiI GAWTC 9 cut(s) 42, 396, 782, 848, 869, 1662, 2144, 2689, 2726
Tru1I TTAA 7 cut(s) 579, 1061, 1871, 1941, 2363, 2520, 2540
Tru9I TTAA 7 cut(s) 579, 1061, 1871, 1941, 2363, 2520, 2540
TscAI CASTG 4 cut(s) 858, 1082, 1362, 2384
TseFI GTSAC 1 cut(s) 2425
TseI GCWGC 6 cut(s) 114, 428, 599, 896, 1730, 2029
Tsp45I GTSAC 1 cut(s) 2425
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 4 cut(s) 858, 1082, 1362, 2384
VneI GTGCAC 1 cut(s) 1215
VpaK11BI GGWCC 7 cut(s) 37, 1001, 1333, 1379, 1877, 1950, 2303
XagI CCTNNNNNAGG 1 cut(s) 1197
XapI RAATTY 5 cut(s) 535, 1319, 1558, 1837, 2525
XceI RCATGY 2 cut(s) 500, 1802
XcmI CCANNNNNNNNNTGG 2 cut(s) 634, 1996
XmaJI CCTAGG 2 cut(s) 791, 2153
XmiI GTMKAC 1 cut(s) 887
XmnI GAANNNNTTC 5 cut(s) 354, 474, 847, 1620, 1776
XspI CTAG 7 cut(s) 168, 792, 998, 1418, 2154, 2243, 2300
Zsp2I ATGCAT 4 cut(s) 210, 502, 1476, 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.