RchiOBHm_Chr5g0019391

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000525
Physical Location & Seq
Reverse (-)
13890799 .. 13904309
13511 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29952

Sequence Viewer

Length: 903 bp
ATGGAGGAACCAATCGGAGAATATTCCTGCCCCGTGCAAATCATCGACTCTGACGGTGATTTCAAGAATGCTGGGCTTGACCGTTTCGTTGAGCAAGTGAAGCTTGGTGAATGCGGGGCCTCATATGTTGTCACCTCCATCATAGGCGCTCAGAGTAGCGGGAAGAGCACCTTAATGAATCATCTTTTCGGCACCGGATTCACTGAGATGGACATAAACAAGAGAAGGTGCCAGACAACAAAGGGGATTTGGATAGCTAAATGTGCTGGTATTGAGCCTTGTACATTGGCCATGGATGTGGAGGGTACGGATGGCAGGGAAAGAGGGGAGGATGATACATTTGAGAGACGAAGTGCGCTATTTGCACTCGCTATTTCGGACATTGTACTAATAAATATGTGGTGCCATGATCTTGGACGAGAGCATGCTGCGAACAAACCTTTACTAAGAACAATTTTTGAGGTTAAGGTTATGATGCATTCATTTGACCCTGCCCGTAAGACAACATTAATATTTATTTTACGGGATAAAACAAAGTCCTCGCTTGAACTTCTCCAACTTGACTTAAAACATGATATACGAAAGATATGGAATGCAGTCCCCAAGTCCAGAGACCATGAAAGCACCCTGCTCGATGAAATTTTCTTTGTAGAAGTAGTTGCTTTGTCCAGTTATGAAGATAAGGAAAAGGAGTTTCAAGAGGAGGTTTCTCAATTGAAGCAACGATTTATCAATTCTACCTCCCCCGGAGGGCTTGCGGGTGGTGACCGAACGGGTGTTCTGCCTGCCTCAGAATTTTCTCATTATGCACAGGAGATTTGGACACAAATAAAAAAGAACAAGGACCTGGATATTGCTTCCCACAAGGTTTTTCGTAATTTTTTTATCTTCCCATTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

300

Amino Acids

33.9

Weight (kDa)

5.49

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
URGCP_GTPase PF25683 42 - 135 4e-06 URGCP-like GTPase domain
RHD3_GTPase PF05879 48 - 285 1.2e-94 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 191, 228, 402
AciI CCGC 3 cut(s) 114, 159, 758
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 2 cut(s) 639, 794
AfaI GTAC 3 cut(s) 283, 307, 387
AfiI CCNNNNNNNGG 1 cut(s) 750
AgsI TTSAA 4 cut(s) 64, 548, 698, 718
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 2 cut(s) 103, 257
AluI AGCT 2 cut(s) 103, 257
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 340, 606
AoxI GGCC 2 cut(s) 117, 288
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 2 cut(s) 639, 794
AseI ATTAAT 1 cut(s) 509
AspLEI GCGC 2 cut(s) 149, 358
AspS9I GGNCC 2 cut(s) 117, 844
AsuC2I CCSGG 1 cut(s) 747
AsuHPI GGTGA 4 cut(s) 68, 119, 124, 776
AvaII GGWCC 1 cut(s) 844
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 3 cut(s) 191, 228, 402
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 415
BccI CCATC 3 cut(s) 146, 202, 305
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BciT130I CCWGG 1 cut(s) 848
BcnI CCSGG 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 340, 606
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 747, 848
Bme18I GGWCC 1 cut(s) 844
BmgT120I GGNCC 2 cut(s) 117, 844
BmiI GGNNCC 5 cut(s) 9, 118, 193, 230, 404
BmrFI CCNGG 2 cut(s) 747, 848
BmsI GCATC 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 291, 745
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 2 cut(s) 291, 745
BseGI GGATG 3 cut(s) 301, 316, 337
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMII CTCAG 3 cut(s) 164, 195, 804
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 716
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 2 cut(s) 119, 290
BshNI GGYRCC 3 cut(s) 191, 228, 402
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 195, 747
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 2 cut(s) 340, 606
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 584
BsmI GAATGC 4 cut(s) 73, 116, 478, 598
BsnI GGCC 2 cut(s) 119, 290
Bso31I GGTCTC 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 281
Bsp143I GATC 1 cut(s) 409
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 3 cut(s) 114, 159, 758
BspANI GGCC 2 cut(s) 119, 290
BspCNI CTCAG 3 cut(s) 163, 196, 803
BspLI GGNNCC 5 cut(s) 9, 118, 193, 230, 404
BspQI GCTCTTC 1 cut(s) 158
BspT107I GGYRCC 3 cut(s) 191, 228, 402
BspTNI GGTCTC 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 281
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 2 cut(s) 291, 745
BssMI GATC 1 cut(s) 409
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 2 cut(s) 56, 83
Bst6I CTCTTC 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 426, 756, 786
BstDEI CTNAG 4 cut(s) 150, 204, 446, 790
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 3 cut(s) 301, 316, 337
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 358
BstKTI GATC 1 cut(s) 412
BstMAI GTCTC 2 cut(s) 340, 606
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 4 cut(s) 100, 165, 263, 362
BstNI CCWGG 1 cut(s) 848
BstNSI RCATGY 1 cut(s) 428
BstPI GGTNACC 1 cut(s) 764
BstSCI CCNGG 2 cut(s) 745, 846
BstV1I GCAGC 1 cut(s) 415
BstXI CCANNNNNNTGG 2 cut(s) 298, 413
BsuRI GGCC 2 cut(s) 119, 290
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 301, 316, 337
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 426, 756, 786
CfoI GCGC 2 cut(s) 149, 358
Cfr13I GGNCC 2 cut(s) 117, 844
Csp6I GTAC 3 cut(s) 282, 306, 386
CviAII CATG 5 cut(s) 292, 407, 425, 572, 617
CviJI RGCY 7 cut(s) 76, 103, 119, 257, 277, 290, 754
CviKI_1 RGCY 7 cut(s) 76, 103, 119, 257, 277, 290, 754
CviQI GTAC 3 cut(s) 282, 306, 386
DdeI CTNAG 4 cut(s) 150, 204, 446, 790
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 844
Eco91I GGTNACC 1 cut(s) 764
EcoO109I RGGNCCY 2 cut(s) 117, 844
EcoO65I GGTNACC 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 846
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 5 cut(s) 295, 410, 428, 575, 620
FalI AAGNNNNNCTT 4 cut(s) 87, 119, 155, 187
FaqI GGGAC 1 cut(s) 584
FatI CATG 5 cut(s) 291, 406, 424, 571, 616
FauI CCCGC 3 cut(s) 107, 152, 751
FauNDI CATATG 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 3 cut(s) 308, 323, 344
Fsp4HI GCNGC 1 cut(s) 429
GlaI GCGC 2 cut(s) 148, 357
GluI GCNGC 1 cut(s) 429
GsaI CCCAGC 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 119, 290
HapII CCGG 2 cut(s) 195, 747
HhaI GCGC 2 cut(s) 149, 358
Hin1II CATG 5 cut(s) 295, 410, 428, 575, 620
Hin6I GCGC 2 cut(s) 147, 356
HinP1I GCGC 2 cut(s) 147, 356
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 47, 178, 198
HpaII CCGG 2 cut(s) 195, 747
HphI GGTGA 4 cut(s) 68, 119, 124, 776
Hpy188I TCNGA 5 cut(s) 17, 52, 153, 379, 793
Hpy188III TCNNGA 3 cut(s) 64, 609, 698
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 2 cut(s) 56, 83
HpyCH4V TGCA 5 cut(s) 37, 365, 478, 596, 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 165, 263, 362
HpyF3I CTNAG 4 cut(s) 150, 204, 446, 790
Hsp92II CATG 5 cut(s) 295, 410, 428, 575, 620
HspAI GCGC 2 cut(s) 147, 356
Kzo9I GATC 1 cut(s) 409
LguI GCTCTTC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 130, 764
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 3 cut(s) 175, 689, 880
MfeI CAATTG 1 cut(s) 713
MhlI GDGCHC 1 cut(s) 170
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 6 cut(s) 453, 639, 713, 733, 794, 877
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 480
MscI TGGCCA 1 cut(s) 290
MseI TTAA 4 cut(s) 173, 465, 509, 566
MslI CAYNNNNRTG 4 cut(s) 173, 206, 296, 898
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 2 cut(s) 195, 747
MspR9I CCNGG 2 cut(s) 747, 848
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 4 cut(s) 73, 116, 478, 598
MvaI CCWGG 1 cut(s) 848
MwoI GCNNNNNNNGC 4 cut(s) 100, 165, 263, 362
NciI CCSGG 1 cut(s) 747
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 124
NdeII GATC 1 cut(s) 409
NlaIII CATG 5 cut(s) 295, 410, 428, 575, 620
NlaIV GGNNCC 5 cut(s) 9, 118, 193, 230, 404
NmuCI GTSAC 2 cut(s) 130, 764
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 1 cut(s) 428
PaeI GCATGC 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 158
PctI GAATGC 4 cut(s) 73, 116, 478, 598
PfeI GAWTC 2 cut(s) 178, 198
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 844
PshBI ATTAAT 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 844
Psp6I CCWGG 1 cut(s) 846
PspEI GGTNACC 1 cut(s) 764
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 5 cut(s) 9, 118, 193, 230, 404
PspPI GGNCC 2 cut(s) 117, 844
PspPPI RGGWCCY 1 cut(s) 844
RsaI GTAC 3 cut(s) 283, 307, 387
RsaNI GTAC 3 cut(s) 282, 306, 386
RseI CAYNNNNRTG 4 cut(s) 173, 206, 296, 898
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 4 cut(s) 173, 465, 509, 566
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 2 cut(s) 117, 844
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 747, 848
SduI GDGCHC 1 cut(s) 170
SfaNI GCATC 1 cut(s) 465
SinI GGWCC 1 cut(s) 844
SmiMI CAYNNNNRTG 4 cut(s) 173, 206, 296, 898
SphI GCATGC 1 cut(s) 428
Sse9I AATT 6 cut(s) 453, 639, 713, 733, 794, 877
SsiI CCGC 3 cut(s) 114, 159, 758
SspI AATATT 2 cut(s) 23, 513
StyD4I CCNGG 2 cut(s) 745, 846
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 56, 83
TaqI TCGA 2 cut(s) 45, 633
TaqII GACCGA 1 cut(s) 783
TasI AATT 6 cut(s) 453, 639, 713, 733, 794, 877
TatI WGTACW 2 cut(s) 281, 385
TfiI GAWTC 2 cut(s) 178, 198
Tru1I TTAA 4 cut(s) 173, 465, 509, 566
Tru9I TTAA 4 cut(s) 173, 465, 509, 566
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 2 cut(s) 130, 764
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 2 cut(s) 130, 764
TspDTI ATGAA 5 cut(s) 191, 471, 633, 651, 690
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 844
VspI ATTAAT 1 cut(s) 509
XapI RAATTY 2 cut(s) 639, 794
XceI RCATGY 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.