Protein ROOT HAIR DEFECTIVE 3 homolog
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1129 |
| AccB1I | GGYRCC | 3 cut(s) | 191, 228, 402 |
| AccII | CGCG | 1 cut(s) | 1359 |
| AccIII | TCCGGA | 1 cut(s) | 1773 |
| AclI | AACGTT | 1 cut(s) | 1549 |
| AclWI | GGATC | 1 cut(s) | 1451 |
| AcoI | YGGCCR | 1 cut(s) | 288 |
| AcsI | RAATTY | 9 cut(s) | 639, 794, 911, 1168, 1300, 1611, 1737, 2121, 2195 |
| AfaI | GTAC | 4 cut(s) | 283, 307, 387, 2115 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 750, 885 |
| AflII | CTTAAG | 2 cut(s) | 1352, 1796 |
| AhlI | ACTAGT | 1 cut(s) | 949 |
| AjnI | CCWGG | 3 cut(s) | 846, 1827, 1997 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 896, 928 |
| Alw21I | GWGCWC | 1 cut(s) | 170 |
| Alw26I | GTCTC | 7 cut(s) | 340, 606, 1034, 1135, 1420, 1829, 1972 |
| AlwI | GGATC | 1 cut(s) | 1451 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1087 |
| Aor13HI | TCCGGA | 1 cut(s) | 1773 |
| AoxI | GGCC | 3 cut(s) | 117, 288, 2205 |
| ApeKI | GCWGC | 8 cut(s) | 27, 428, 919, 1081, 1292, 1817, 2134, 2137 |
| ApoI | RAATTY | 9 cut(s) | 639, 794, 911, 1168, 1300, 1611, 1737, 2121, 2195 |
| AseI | ATTAAT | 1 cut(s) | 509 |
| AspLEI | GCGC | 4 cut(s) | 149, 358, 1359, 2223 |
| AspS9I | GGNCC | 3 cut(s) | 117, 844, 1582 |
| AsuC2I | CCSGG | 1 cut(s) | 747 |
| AsuHPI | GGTGA | 7 cut(s) | 68, 119, 124, 776, 1105, 1496, 2044 |
| AvaII | GGWCC | 2 cut(s) | 844, 1582 |
| BaeGI | GKGCMC | 1 cut(s) | 1050 |
| BalI | TGGCCA | 1 cut(s) | 290 |
| BanI | GGYRCC | 3 cut(s) | 191, 228, 402 |
| BauI | CACGAG | 1 cut(s) | 1668 |
| Bbv12I | GWGCWC | 1 cut(s) | 170 |
| BbvI | GCAGC | 8 cut(s) | 14, 415, 931, 1093, 1279, 1804, 2121, 2124 |
| BccI | CCATC | 4 cut(s) | 146, 202, 305, 1874 |
| BceAI | ACGGC | 1 cut(s) | 1094 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 613, 647, 1263, 1297, 1706, 1740 |
| BciT130I | CCWGG | 3 cut(s) | 848, 1829, 1999 |
| BcnI | CCSGG | 1 cut(s) | 747 |
| BcoDI | GTCTC | 7 cut(s) | 340, 606, 1034, 1135, 1420, 1829, 1972 |
| BcuI | ACTAGT | 1 cut(s) | 949 |
| BfaI | CTAG | 6 cut(s) | 368, 950, 1205, 1734, 2126, 2183 |
| BfmI | CTRYAG | 3 cut(s) | 648, 1461, 2209 |
| BfoI | RGCGCY | 2 cut(s) | 150, 2224 |
| BfrI | CTTAAG | 2 cut(s) | 1352, 1796 |
| BfuAI | ACCTGC | 1 cut(s) | 1129 |
| BisI | GCNGC | 9 cut(s) | 28, 429, 920, 1082, 1293, 1818, 2103, 2135, 2138 |
| BlpI | GCTNAGC | 2 cut(s) | 923, 998 |
| BlsI | GCNGC | 9 cut(s) | 29, 430, 921, 1083, 1294, 1819, 2104, 2136, 2139 |
| Bme1390I | CCNGG | 4 cut(s) | 747, 848, 1829, 1999 |
| Bme18I | GGWCC | 2 cut(s) | 844, 1582 |
| BmgT120I | GGNCC | 3 cut(s) | 117, 844, 1582 |
| BmiI | GGNNCC | 5 cut(s) | 9, 118, 193, 230, 404 |
| BmrFI | CCNGG | 4 cut(s) | 747, 848, 1829, 1999 |
| BmsI | GCATC | 7 cut(s) | 465, 1267, 1279, 1385, 1516, 1714, 2114 |
| BpmI | CTGGAG | 2 cut(s) | 1519, 1979 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 923, 998 |
| BpuEI | CTTGAG | 2 cut(s) | 1349, 1499 |
| BpuMI | CCSGG | 1 cut(s) | 747 |
| Bsa29I | ATCGAT | 2 cut(s) | 872, 1878 |
| BsaI | GGTCTC | 1 cut(s) | 606 |
| BsaJI | CCNNGG | 3 cut(s) | 291, 745, 1585 |
| BsaWI | WCCGGW | 2 cut(s) | 194, 1773 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1952, 1982 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 750, 885 |
| Bse1I | ACTGG | 4 cut(s) | 669, 1188, 1419, 1966 |
| BseAI | TCCGGA | 1 cut(s) | 1773 |
| BseBI | CCWGG | 3 cut(s) | 848, 1829, 1999 |
| BseCI | ATCGAT | 2 cut(s) | 872, 1878 |
| BseDI | CCNNGG | 3 cut(s) | 291, 745, 1585 |
| BseGI | GGATG | 5 cut(s) | 301, 316, 337, 1220, 1837 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 750, 885 |
| BseMII | CTCAG | 8 cut(s) | 164, 195, 804, 937, 1012, 1130, 1923, 2138 |
| BseNI | ACTGG | 4 cut(s) | 669, 1188, 1419, 1966 |
| BseRI | GAGGAG | 1 cut(s) | 716 |
| BseSI | GKGCMC | 1 cut(s) | 1050 |
| BseXI | GCAGC | 8 cut(s) | 14, 415, 931, 1093, 1279, 1804, 2121, 2124 |
| BsgI | GTGCAG | 2 cut(s) | 1293, 2210 |
| Bsh1236I | CGCG | 1 cut(s) | 1359 |
| BshFI | GGCC | 3 cut(s) | 119, 290, 2207 |
| BshNI | GGYRCC | 3 cut(s) | 191, 228, 402 |
| BshVI | ATCGAT | 2 cut(s) | 872, 1878 |
| BsiHKAI | GWGCWC | 1 cut(s) | 170 |
| BsiSI | CCGG | 3 cut(s) | 195, 747, 1774 |
| BslFI | GGGAC | 4 cut(s) | 584, 1527, 1634, 1777 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 750, 885 |
| BsmAI | GTCTC | 7 cut(s) | 340, 606, 1034, 1135, 1420, 1829, 1972 |
| BsmBI | CGTCTC | 2 cut(s) | 340, 1829 |
| BsmFI | GGGAC | 4 cut(s) | 584, 1527, 1634, 1777 |
| BsmI | GAATGC | 5 cut(s) | 116, 478, 598, 1318, 1783 |
| BsnI | GGCC | 3 cut(s) | 119, 290, 2207 |
| Bso31I | GGTCTC | 1 cut(s) | 606 |
| Bsp1286I | GDGCHC | 2 cut(s) | 170, 1050 |
| Bsp13I | TCCGGA | 1 cut(s) | 1773 |
| Bsp1407I | TGTACA | 1 cut(s) | 281 |
| Bsp143I | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 923, 998 |
| Bsp19I | CCATGG | 1 cut(s) | 291 |
| BspANI | GGCC | 3 cut(s) | 119, 290, 2207 |
| BspCNI | CTCAG | 8 cut(s) | 163, 196, 803, 936, 1011, 1131, 1922, 2139 |
| BspDI | ATCGAT | 2 cut(s) | 872, 1878 |
| BspEI | TCCGGA | 1 cut(s) | 1773 |
| BspFNI | CGCG | 1 cut(s) | 1359 |
| BspHI | TCATGA | 1 cut(s) | 1522 |
| BspLI | GGNNCC | 5 cut(s) | 9, 118, 193, 230, 404 |
| BspMI | ACCTGC | 1 cut(s) | 1129 |
| BspPI | GGATC | 1 cut(s) | 1451 |
| BspQI | GCTCTTC | 1 cut(s) | 158 |
| BspT107I | GGYRCC | 3 cut(s) | 191, 228, 402 |
| BspTI | CTTAAG | 2 cut(s) | 1352, 1796 |
| BspTNI | GGTCTC | 1 cut(s) | 606 |
| BsrGI | TGTACA | 1 cut(s) | 281 |
| BsrI | ACTGG | 4 cut(s) | 669, 1188, 1419, 1966 |
| BssECI | CCNNGG | 3 cut(s) | 291, 745, 1585 |
| BssMI | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| BssSI | CACGAG | 1 cut(s) | 1668 |
| BssT1I | CCWWGG | 2 cut(s) | 291, 1585 |
| Bst2BI | CACGAG | 1 cut(s) | 1668 |
| Bst2UI | CCWGG | 3 cut(s) | 848, 1829, 1999 |
| Bst4CI | ACNGT | 8 cut(s) | 56, 961, 1089, 1398, 1421, 1625, 1983, 2062 |
| Bst6I | CTCTTC | 4 cut(s) | 158, 888, 1627, 1870 |
| BstAFI | CTTAAG | 2 cut(s) | 1352, 1796 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1615 |
| BstAUI | TGTACA | 1 cut(s) | 281 |
| BstC8I | GCNNGC | 5 cut(s) | 426, 756, 786, 1250, 2004 |
| BstDEI | CTNAG | 9 cut(s) | 150, 204, 446, 790, 923, 998, 1139, 1909, 2147 |
| BstDSI | CCRYGG | 1 cut(s) | 291 |
| BstEII | GGTNACC | 1 cut(s) | 764 |
| BstF5I | GGATG | 5 cut(s) | 301, 316, 337, 1220, 1837 |
| BstFNI | CGCG | 1 cut(s) | 1359 |
| BstH2I | RGCGCY | 2 cut(s) | 150, 2224 |
| BstHHI | GCGC | 4 cut(s) | 149, 358, 1359, 2223 |
| BstKTI | GATC | 4 cut(s) | 412, 973, 1193, 1459 |
| BstMAI | GTCTC | 7 cut(s) | 340, 606, 1034, 1135, 1420, 1829, 1972 |
| BstMBI | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 100, 165, 263, 362, 1615 |
| BstNI | CCWGG | 3 cut(s) | 848, 1829, 1999 |
| BstNSI | RCATGY | 4 cut(s) | 428, 1252, 1272, 2006 |
| BstPI | GGTNACC | 1 cut(s) | 764 |
| BstSCI | CCNGG | 4 cut(s) | 745, 846, 1827, 1997 |
| BstSFI | CTRYAG | 3 cut(s) | 648, 1461, 2209 |
| BstSLI | GKGCMC | 1 cut(s) | 1050 |
| BstUI | CGCG | 1 cut(s) | 1359 |
| BstV1I | GCAGC | 8 cut(s) | 14, 415, 931, 1093, 1279, 1804, 2121, 2124 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 298, 413, 1828 |
| Bsu15I | ATCGAT | 2 cut(s) | 872, 1878 |
| BsuRI | GGCC | 3 cut(s) | 119, 290, 2207 |
| BsuTUI | ATCGAT | 2 cut(s) | 872, 1878 |
| BtgI | CCRYGG | 1 cut(s) | 291 |
| BtsCI | GGATG | 5 cut(s) | 301, 316, 337, 1220, 1837 |
| BtsIMutI | CAGTG | 1 cut(s) | 201 |
| BveI | ACCTGC | 1 cut(s) | 1129 |
| Cac8I | GCNNGC | 5 cut(s) | 426, 756, 786, 1250, 2004 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1087 |
| CciI | TCATGA | 1 cut(s) | 1522 |
| CfoI | GCGC | 4 cut(s) | 149, 358, 1359, 2223 |
| Cfr13I | GGNCC | 3 cut(s) | 117, 844, 1582 |
| ClaI | ATCGAT | 2 cut(s) | 872, 1878 |
| CseI | GACGC | 1 cut(s) | 1846 |
| Csp6I | GTAC | 4 cut(s) | 282, 306, 386, 2114 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1617, 1652, 1885, 1920 |
| CviQI | GTAC | 4 cut(s) | 282, 306, 386, 2114 |
| DdeI | CTNAG | 9 cut(s) | 150, 204, 446, 790, 923, 998, 1139, 1909, 2147 |
| DpnI | GATC | 4 cut(s) | 411, 972, 1192, 1458 |
| DpnII | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| DraI | TTTAAA | 1 cut(s) | 1174 |
| EaeI | YGGCCR | 1 cut(s) | 288 |
| Eam1104I | CTCTTC | 4 cut(s) | 158, 888, 1627, 1870 |
| EarI | CTCTTC | 4 cut(s) | 158, 888, 1627, 1870 |
| EciI | GGCGGA | 1 cut(s) | 1655 |
| Eco130I | CCWWGG | 2 cut(s) | 291, 1585 |
| Eco147I | AGGCCT | 1 cut(s) | 2207 |
| Eco31I | GGTCTC | 1 cut(s) | 606 |
| Eco47I | GGWCC | 2 cut(s) | 844, 1582 |
| Eco91I | GGTNACC | 1 cut(s) | 764 |
| EcoO109I | RGGNCCY | 2 cut(s) | 117, 844 |
| EcoO65I | GGTNACC | 1 cut(s) | 764 |
| EcoRII | CCWGG | 3 cut(s) | 846, 1827, 1997 |
| EcoT14I | CCWWGG | 2 cut(s) | 291, 1585 |
| EcoT22I | ATGCAT | 3 cut(s) | 480, 1318, 1378 |
| ErhI | CCWWGG | 2 cut(s) | 291, 1585 |
| Esp3I | CGTCTC | 2 cut(s) | 340, 1829 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 87, 119, 155, 187 |
| FaqI | GGGAC | 4 cut(s) | 584, 1527, 1634, 1777 |
| FauI | CCCGC | 4 cut(s) | 107, 152, 751, 1611 |
| FauNDI | CATATG | 3 cut(s) | 124, 1318, 1372 |
| Fnu4HI | GCNGC | 9 cut(s) | 28, 429, 920, 1082, 1293, 1818, 2103, 2135, 2138 |
| FokI | GGATG | 5 cut(s) | 308, 323, 344, 1207, 1844 |
| Fsp4HI | GCNGC | 9 cut(s) | 28, 429, 920, 1082, 1293, 1818, 2103, 2135, 2138 |
| FspBI | CTAG | 6 cut(s) | 368, 950, 1205, 1734, 2126, 2183 |
| GlaI | GCGC | 4 cut(s) | 148, 357, 1358, 2222 |
| GluI | GCNGC | 9 cut(s) | 28, 429, 920, 1082, 1293, 1818, 2103, 2135, 2138 |
| GsuI | CTGGAG | 2 cut(s) | 1519, 1979 |
| HaeII | RGCGCY | 2 cut(s) | 150, 2224 |
| HaeIII | GGCC | 3 cut(s) | 119, 290, 2207 |
| HapII | CCGG | 3 cut(s) | 195, 747, 1774 |
| HgaI | GACGC | 1 cut(s) | 1846 |
| HhaI | GCGC | 4 cut(s) | 149, 358, 1359, 2223 |
| Hin6I | GCGC | 4 cut(s) | 147, 356, 1357, 2221 |
| HinP1I | GCGC | 4 cut(s) | 147, 356, 1357, 2221 |
| HincII | GTYRAC | 2 cut(s) | 1390, 1756 |
| HindII | GTYRAC | 2 cut(s) | 1390, 1756 |
| HindIII | AAGCTT | 3 cut(s) | 101, 1440, 2016 |
| HpaII | CCGG | 3 cut(s) | 195, 747, 1774 |
| HphI | GGTGA | 7 cut(s) | 68, 119, 124, 776, 1105, 1496, 2044 |
| Hpy166II | GTNNAC | 2 cut(s) | 1390, 1756 |
| Hpy188I | TCNGA | 9 cut(s) | 17, 52, 153, 379, 793, 1156, 1202, 1677, 1703 |
| Hpy8I | GTNNAC | 2 cut(s) | 1390, 1756 |
| Hpy99I | CGWCG | 1 cut(s) | 1493 |
| HpyAV | CCTTC | 3 cut(s) | 219, 1202, 1717 |
| HpyCH4III | ACNGT | 8 cut(s) | 56, 961, 1089, 1398, 1421, 1625, 1983, 2062 |
| HpyCH4IV | ACGT | 1 cut(s) | 1549 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 100, 165, 263, 362, 1615 |
| HpyF3I | CTNAG | 9 cut(s) | 150, 204, 446, 790, 923, 998, 1139, 1909, 2147 |
| HpySE526I | ACGT | 1 cut(s) | 1549 |
| HspAI | GCGC | 4 cut(s) | 147, 356, 1357, 2221 |
| Kpn2I | TCCGGA | 1 cut(s) | 1773 |
| Kzo9I | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| LguI | GCTCTTC | 1 cut(s) | 158 |
| Lsp1109I | GCAGC | 8 cut(s) | 14, 415, 931, 1093, 1279, 1804, 2121, 2124 |
| LweI | GCATC | 7 cut(s) | 465, 1267, 1279, 1385, 1516, 1714, 2114 |
| MaeI | CTAG | 6 cut(s) | 368, 950, 1205, 1734, 2126, 2183 |
| MaeII | ACGT | 1 cut(s) | 1549 |
| MaeIII | GTNAC | 5 cut(s) | 130, 764, 952, 1093, 1484 |
| MalI | GATC | 4 cut(s) | 411, 972, 1192, 1458 |
| MboI | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| MboII | GAAGA | 9 cut(s) | 175, 647, 689, 905, 1511, 1602, 1644, 1857, 1923 |
| MfeI | CAATTG | 1 cut(s) | 713 |
| MhlI | GDGCHC | 2 cut(s) | 170, 1050 |
| MlsI | TGGCCA | 1 cut(s) | 290 |
| MluNI | TGGCCA | 1 cut(s) | 290 |
| MlyI | GAGTC | 5 cut(s) | 41, 1672, 1799, 1866, 1901 |
| MmeI | TCCRAC | 3 cut(s) | 580, 1225, 1560 |
| Mox20I | TGGCCA | 1 cut(s) | 290 |
| Mph1103I | ATGCAT | 3 cut(s) | 480, 1318, 1378 |
| MroI | TCCGGA | 1 cut(s) | 1773 |
| MscI | TGGCCA | 1 cut(s) | 290 |
| MslI | CAYNNNNRTG | 3 cut(s) | 173, 206, 296 |
| Msp20I | TGGCCA | 1 cut(s) | 290 |
| MspA1I | CMGCKG | 1 cut(s) | 1957 |
| MspCI | CTTAAG | 2 cut(s) | 1352, 1796 |
| MspI | CCGG | 3 cut(s) | 195, 747, 1774 |
| MspR9I | CCNGG | 4 cut(s) | 747, 848, 1829, 1999 |
| MunI | CAATTG | 1 cut(s) | 713 |
| Mva1269I | GAATGC | 5 cut(s) | 116, 478, 598, 1318, 1783 |
| MvaI | CCWGG | 3 cut(s) | 848, 1829, 1999 |
| MvnI | CGCG | 1 cut(s) | 1359 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 100, 165, 263, 362, 1615 |
| NciI | CCSGG | 1 cut(s) | 747 |
| NcoI | CCATGG | 1 cut(s) | 291 |
| NdeI | CATATG | 3 cut(s) | 124, 1318, 1372 |
| NdeII | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| NlaIV | GGNNCC | 5 cut(s) | 9, 118, 193, 230, 404 |
| NmuCI | GTSAC | 4 cut(s) | 130, 764, 1093, 1484 |
| NsiI | ATGCAT | 3 cut(s) | 480, 1318, 1378 |
| NspI | RCATGY | 4 cut(s) | 428, 1252, 1272, 2006 |
| PaeI | GCATGC | 3 cut(s) | 428, 1252, 2006 |
| PagI | TCATGA | 1 cut(s) | 1522 |
| PceI | AGGCCT | 1 cut(s) | 2207 |
| PciSI | GCTCTTC | 1 cut(s) | 158 |
| PctI | GAATGC | 5 cut(s) | 116, 478, 598, 1318, 1783 |
| PfeI | GAWTC | 5 cut(s) | 178, 198, 1241, 2035, 2091 |
| PkrI | GCNGC | 9 cut(s) | 29, 430, 921, 1083, 1294, 1819, 2104, 2136, 2139 |
| PleI | GAGTC | 5 cut(s) | 41, 1672, 1798, 1865, 1901 |
| PpsI | GAGTC | 5 cut(s) | 41, 1672, 1798, 1865, 1901 |
| PpuMI | RGGWCCY | 1 cut(s) | 844 |
| PshBI | ATTAAT | 1 cut(s) | 509 |
| Psp1406I | AACGTT | 1 cut(s) | 1549 |
| Psp5II | RGGWCCY | 1 cut(s) | 844 |
| Psp6I | CCWGG | 3 cut(s) | 846, 1827, 1997 |
| PspEI | GGTNACC | 1 cut(s) | 764 |
| PspGI | CCWGG | 3 cut(s) | 846, 1827, 1997 |
| PspN4I | GGNNCC | 5 cut(s) | 9, 118, 193, 230, 404 |
| PspPI | GGNCC | 3 cut(s) | 117, 844, 1582 |
| PspPPI | RGGWCCY | 1 cut(s) | 844 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 945, 977 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1087 |
| RsaI | GTAC | 4 cut(s) | 283, 307, 387, 2115 |
| RsaNI | GTAC | 4 cut(s) | 282, 306, 386, 2114 |
| RseI | CAYNNNNRTG | 3 cut(s) | 173, 206, 296 |
| SapI | GCTCTTC | 1 cut(s) | 158 |
| SatI | GCNGC | 9 cut(s) | 28, 429, 920, 1082, 1293, 1818, 2103, 2135, 2138 |
| Sau3AI | GATC | 4 cut(s) | 409, 970, 1190, 1456 |
| Sau96I | GGNCC | 3 cut(s) | 117, 844, 1582 |
| SchI | GAGTC | 5 cut(s) | 41, 1672, 1799, 1866, 1901 |
| ScrFI | CCNGG | 4 cut(s) | 747, 848, 1829, 1999 |
| SduI | GDGCHC | 2 cut(s) | 170, 1050 |
| SfaNI | GCATC | 7 cut(s) | 465, 1267, 1279, 1385, 1516, 1714, 2114 |
| SfcI | CTRYAG | 3 cut(s) | 648, 1461, 2209 |
| SinI | GGWCC | 2 cut(s) | 844, 1582 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 173, 206, 296 |
| SmlI | CTYRAG | 4 cut(s) | 1328, 1352, 1478, 1796 |
| SmoI | CTYRAG | 4 cut(s) | 1328, 1352, 1478, 1796 |
| SpeI | ACTAGT | 1 cut(s) | 949 |
| SphI | GCATGC | 3 cut(s) | 428, 1252, 2006 |
| SseBI | AGGCCT | 1 cut(s) | 2207 |
| SspI | AATATT | 2 cut(s) | 23, 513 |
| SspMI | CTAG | 6 cut(s) | 368, 950, 1205, 1734, 2126, 2183 |
| StuI | AGGCCT | 1 cut(s) | 2207 |
| StyD4I | CCNGG | 4 cut(s) | 745, 846, 1827, 1997 |
| StyI | CCWWGG | 2 cut(s) | 291, 1585 |
| TaaI | ACNGT | 8 cut(s) | 56, 961, 1089, 1398, 1421, 1625, 1983, 2062 |
| TaiI | ACGT | 1 cut(s) | 1552 |
| TaqI | TCGA | 6 cut(s) | 45, 633, 872, 1073, 1283, 1878 |
| TaqII | GACCGA | 1 cut(s) | 783 |
| TatI | WGTACW | 2 cut(s) | 281, 385 |
| TauI | GCSGC | 1 cut(s) | 2105 |
| TfiI | GAWTC | 5 cut(s) | 178, 198, 1241, 2035, 2091 |
| TscAI | CASTG | 1 cut(s) | 208 |
| TseFI | GTSAC | 4 cut(s) | 130, 764, 1093, 1484 |
| TseI | GCWGC | 8 cut(s) | 27, 428, 919, 1081, 1292, 1817, 2134, 2137 |
| Tsp45I | GTSAC | 4 cut(s) | 130, 764, 1093, 1484 |
| TspDTI | ATGAA | 8 cut(s) | 191, 471, 633, 690, 904, 1209, 1511, 1560 |
| TspGWI | ACGGA | 4 cut(s) | 323, 1139, 1213, 2103 |
| TspRI | CASTG | 1 cut(s) | 208 |
| Vha464I | CTTAAG | 2 cut(s) | 1352, 1796 |
| VpaK11BI | GGWCC | 2 cut(s) | 844, 1582 |
| VspI | ATTAAT | 1 cut(s) | 509 |
| XapI | RAATTY | 9 cut(s) | 639, 794, 911, 1168, 1300, 1611, 1737, 2121, 2195 |
| XceI | RCATGY | 4 cut(s) | 428, 1252, 1272, 2006 |
| XspI | CTAG | 6 cut(s) | 368, 950, 1205, 1734, 2126, 2183 |
| Zsp2I | ATGCAT | 3 cut(s) | 480, 1318, 1378 |