MD10G1213100.v1.1

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000525
Physical Location & Seq
Reverse (-)
31169368 .. 31170852
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1213100.v1.1.491

Sequence Viewer

Length: 441 bp
ATGAACTCGATGAGAAGCCACCATCTCTTATCTGCAAAGCGAAGAAGAACTCGACAGATTGTATATGGGCTCGACAGGTTTGTGAAGGAGGTGAAGCTTACCGAGTGCGGACTCTCCTATGCGGTTGTTGTAATCATGGGTCCGCAGAGCAGCGGGAAAAGCACTTTGTTAAATCATTTGTTTTGGACTAAGTTTAGGGAGATGGATGCTTACGGTGGAAGGAGCCAAACTACAAAGGGTATTTGGATTGCCAGGTGTGTTGGCATTGAGCTGTGCACGATTGCGATGGATTTGGAGGGGACTGATGGGAGGGAGAGGGGCGAGGTAGTCAGATTGATTTTTTTTAGGGGGTTTGTGGGGTTTTTTTTTAGAGATTTTTCTGTGAAAGCATTGTTGTTGTATGAATTTTTTTTATTTTGGGTTTTGATTGTTTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

147

Amino Acids

16.99

Weight (kDa)

9.81

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RHD3_GTPase PF05879 46 - 108 9.5e-28 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 108, 122, 143, 153
AcsI RAATTY 1 cut(s) 404
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 2 cut(s) 97, 271
AluI AGCT 2 cut(s) 97, 271
Alw21I GWGCWC 1 cut(s) 278
Alw44I GTGCAC 1 cut(s) 274
ApaLI GTGCAC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 1 cut(s) 162
BccI CCATC 4 cut(s) 30, 196, 280, 299
BciT130I CCWGG 1 cut(s) 253
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 141, 224
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 196
BseBI CCWGG 1 cut(s) 253
BseGI GGATG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 278
BseXI GCAGC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 278
BslFI GGGAC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 72, 278
BspACI CCGC 4 cut(s) 108, 122, 143, 153
BspLI GGNNCC 2 cut(s) 141, 224
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 211
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 140
CviAII CATG 1 cut(s) 136
CviJI RGCY 5 cut(s) 18, 70, 97, 225, 271
CviKI_1 RGCY 5 cut(s) 18, 70, 97, 225, 271
DdeI CTNAG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 72
FaeI CATG 1 cut(s) 139
FaiI YATR 6 cut(s) 64, 66, 120, 137, 402, 439
FaqI GGGAC 1 cut(s) 313
FatI CATG 1 cut(s) 135
FauI CCCGC 1 cut(s) 146
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 151
GluI GCNGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 1 cut(s) 111
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 79, 213
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 35, 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 1 cut(s) 189
Hsp92II CATG 1 cut(s) 139
LmnI GCTCC 1 cut(s) 222
LpnPI CCDG 3 cut(s) 61, 238, 265
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 196
MboII GAAGA 2 cut(s) 54, 57
MhlI GDGCHC 2 cut(s) 72, 278
MluCI AATT 1 cut(s) 404
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 5 cut(s) 82, 289, 303, 309, 316
MseI TTAA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 159
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 2 cut(s) 141, 224
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 141, 224
PspPI GGNCC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 2 cut(s) 72, 278
SetI ASST 6 cut(s) 80, 93, 99, 257, 273, 327
SfaNI GCATC 1 cut(s) 196
SinI GGWCC 1 cut(s) 140
Sse9I AATT 1 cut(s) 404
SsiI CCGC 4 cut(s) 108, 122, 143, 153
StyD4I CCNGG 1 cut(s) 251
TaaI ACNGT 1 cut(s) 215
TaqI TCGA 3 cut(s) 8, 52, 72
TasI AATT 1 cut(s) 404
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TseI GCWGC 1 cut(s) 150
TspDTI ATGAA 2 cut(s) 17, 417
VneI GTGCAC 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.