RchiOBHm_Chr7g0226161

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000525
Physical Location & Seq
Forward (+)
49223297 .. 49229006
5710 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20256

Sequence Viewer

Length: 786 bp
ATGGTTGAAAATAGTTGTTCTCTCAAGAAAGTAGTACTAGAAATTGTCTACACATCTGATGGGTGTGGGAGCTGTGAGTATGCTTGTAAGAGTTGTGATGGTTACACTTGCGTGGAAGTAGTTGCTTTGTCTCATTATGTTTACGAGAGGAAGAAGTTTGAGGAGGAGGTTGCTGAATTGAGGCAGCGGTTTTCCAATTCTATTTCTCCGGAAGGGCTTGCTGGTGGTGACAGAAATGGCGTTGTCCCTGCCTCGGGATTTTCTTTTAGTGCTCAGCATATTTGGAAACAAATCAAACAGAACAAAGACCTGGATCTTCCTTCTCTCAATGTTATGGTTGCCAACGTTCGGTGTGAGGAGATAGCGAACGAGATATTCAATCAGTTGACAACAAATCAGGATTGGTTGAAACTGGAAGAAGCTGTCAAAATTGGTATTGTAGAAGGTTTTGGTGAAATGTTTGTGCACCCAGCGTACACTACCCTGATCAACAACATACGTTTCAGAACGTTTAAAGAGTTCAAAGTGAAACTGCAACAGGCATTAGACAATAAAGAAGACATAGATGCATCTGTTCAGACCCTCACTGAGTCTTCCATTATTGAGTTTAACCATGGATGTACAGATGCTGCCATAAAAGATGCTAATTGGGATGTTTCAAGCACTCGAAATATACTTAGCTCCGATATAAAGGGACATGCAGAAACTGCTTCCGTCTTCGAAGATTTGAAAAGAGTGAATCTCAAGGACAAGGTAACTATATATTACACACACACACACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

261

Amino Acids

29.38

Weight (kDa)

5.24

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RHD3_GTPase PF05879 33 - 107 4.5e-20 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 154 - 239 1.2e-10 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 1 cut(s) 187
AclI AACGTT 2 cut(s) 345, 509
AclWI GGATC 1 cut(s) 321
AfaI GTAC 3 cut(s) 36, 476, 622
AfiI CCNNNNNNNGG 3 cut(s) 253, 254, 348
AgsI TTSAA 6 cut(s) 8, 379, 409, 523, 660, 730
AjnI CCWGG 1 cut(s) 309
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 3 cut(s) 72, 422, 681
AluI AGCT 3 cut(s) 72, 422, 681
Alw21I GWGCWC 2 cut(s) 274, 468
Alw26I GTCTC 1 cut(s) 135
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 1 cut(s) 321
AlwNI CAGNNNCTG 2 cut(s) 629, 707
Ama87I CYCGRG 1 cut(s) 253
Aor13HI TCCGGA 1 cut(s) 208
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 2 cut(s) 184, 629
AsuHPI GGTGA 2 cut(s) 239, 464
AsuII TTCGAA 1 cut(s) 720
AvaI CYCGRG 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 468
BbsI GAAGAC 3 cut(s) 564, 585, 709
Bbv12I GWGCWC 2 cut(s) 274, 468
BbvI GCAGC 2 cut(s) 196, 616
BccI CCATC 2 cut(s) 53, 92
BciT130I CCWGG 1 cut(s) 311
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 38
BisI GCNGC 2 cut(s) 185, 630
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 2 cut(s) 186, 631
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 311
BmeT110I CYCGRG 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 4 cut(s) 556, 578, 616, 631
BpiI GAAGAC 3 cut(s) 564, 585, 709
BplI GAGNNNNNCTC 2 cut(s) 726, 758
Bpu1102I GCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 2 cut(s) 8, 728
BsaJI CCNNGG 2 cut(s) 252, 613
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 254, 348
Bse1I ACTGG 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 252, 613
BseGI GGATG 2 cut(s) 623, 658
BseLI CCNNNNNNNGG 3 cut(s) 253, 254, 348
BseMII CTCAG 2 cut(s) 287, 579
BseNI ACTGG 1 cut(s) 417
BseRI GAGGAG 3 cut(s) 176, 179, 371
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 2 cut(s) 196, 616
BseYI CCCAGC 1 cut(s) 469
BsiHKAI GWGCWC 2 cut(s) 274, 468
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 2 cut(s) 230, 708
BslI CCNNNNNNNGG 3 cut(s) 253, 254, 348
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 230, 708
BsoBI CYCGRG 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 274, 468
Bsp13I TCCGGA 1 cut(s) 208
Bsp1407I TGTACA 1 cut(s) 620
Bsp143I GATC 2 cut(s) 313, 486
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 613
BspACI CCGC 1 cut(s) 187
BspCNI CTCAG 2 cut(s) 286, 580
BspEI TCCGGA 1 cut(s) 208
BspPI GGATC 1 cut(s) 321
BspT104I TTCGAA 1 cut(s) 720
BsrGI TGTACA 1 cut(s) 620
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 252, 613
BssMI GATC 2 cut(s) 313, 486
BssT1I CCWWGG 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 707
BstAUI TGTACA 1 cut(s) 620
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 273, 588, 677
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 2 cut(s) 623, 658
BstKTI GATC 2 cut(s) 316, 489
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 313, 486
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 468
BstV1I GCAGC 2 cut(s) 196, 616
BstV2I GAAGAC 3 cut(s) 564, 585, 709
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 2 cut(s) 623, 658
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 1 cut(s) 219
CaiI CAGNNNCTG 2 cut(s) 629, 707
Csp6I GTAC 3 cut(s) 35, 475, 621
CviAII CATG 2 cut(s) 614, 698
CviJI RGCY 4 cut(s) 72, 217, 422, 681
CviKI_1 RGCY 4 cut(s) 72, 217, 422, 681
CviQI GTAC 3 cut(s) 35, 475, 621
DdeI CTNAG 3 cut(s) 273, 588, 677
DpnI GATC 2 cut(s) 315, 488
DpnII GATC 2 cut(s) 313, 486
DraI TTTAAA 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 2 cut(s) 617, 701
FaqI GGGAC 2 cut(s) 230, 708
FatI CATG 2 cut(s) 613, 697
FbaI TGATCA 1 cut(s) 486
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 2 cut(s) 185, 630
FokI GGATG 2 cut(s) 630, 665
Fsp4HI GCNGC 2 cut(s) 185, 630
FspBI CTAG 1 cut(s) 38
GluI GCNGC 2 cut(s) 185, 630
GsaI CCCAGC 1 cut(s) 473
HapII CCGG 1 cut(s) 209
Hin1II CATG 2 cut(s) 617, 701
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 2 cut(s) 590, 739
HpaII CCGG 1 cut(s) 209
HphI GGTGA 2 cut(s) 239, 464
Hpy166II GTNNAC 5 cut(s) 49, 142, 387, 466, 477
Hpy188I TCNGA 4 cut(s) 58, 506, 579, 685
Hpy188III TCNNGA 4 cut(s) 25, 209, 255, 398
Hpy8I GTNNAC 5 cut(s) 49, 142, 387, 466, 477
HpyAV CCTTC 3 cut(s) 206, 330, 437
HpyCH4IV ACGT 3 cut(s) 345, 499, 509
HpyCH4V TGCA 4 cut(s) 466, 535, 569, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 3 cut(s) 273, 588, 677
HpySE526I ACGT 3 cut(s) 345, 499, 509
Hsp92II CATG 2 cut(s) 617, 701
Kpn2I TCCGGA 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 2 cut(s) 313, 486
LmnI GCTCC 2 cut(s) 69, 686
Lsp1109I GCAGC 2 cut(s) 196, 616
LweI GCATC 4 cut(s) 556, 578, 616, 631
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 345, 499, 509
MaeIII GTNAC 3 cut(s) 101, 227, 754
MalI GATC 2 cut(s) 315, 488
MboI GATC 2 cut(s) 313, 486
MboII GAAGA 7 cut(s) 163, 308, 428, 569, 585, 709, 734
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 274, 468
MluCI AATT 5 cut(s) 42, 176, 196, 429, 646
MlyI GAGTC 1 cut(s) 599
MnlI CCTC 8 cut(s) 141, 154, 157, 160, 174, 262, 349, 593
Mph1103I ATGCAT 1 cut(s) 571
MroI TCCGGA 1 cut(s) 208
MseI TTAA 2 cut(s) 513, 609
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 707
NcoI CCATGG 1 cut(s) 613
NdeII GATC 2 cut(s) 313, 486
NlaIII CATG 2 cut(s) 617, 701
NmuCI GTSAC 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 571
NspI RCATGY 1 cut(s) 701
NspV TTCGAA 1 cut(s) 720
OliI CACNNNNGTG 1 cut(s) 110
PfeI GAWTC 1 cut(s) 739
PkrI GCNGC 2 cut(s) 186, 631
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Psp1406I AACGTT 2 cut(s) 345, 509
Psp6I CCWGG 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 309
PstNI CAGNNNCTG 2 cut(s) 629, 707
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 3 cut(s) 36, 476, 622
RsaNI GTAC 3 cut(s) 35, 475, 621
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 2 cut(s) 513, 609
SatI GCNGC 2 cut(s) 185, 630
Sau3AI GATC 2 cut(s) 313, 486
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 2 cut(s) 274, 468
SfaNI GCATC 4 cut(s) 556, 578, 616, 631
SfuI TTCGAA 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 23, 743
SmoI CTYRAG 2 cut(s) 23, 743
Sse9I AATT 5 cut(s) 42, 176, 196, 429, 646
SsiI CCGC 1 cut(s) 187
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 613
TaiI ACGT 3 cut(s) 348, 502, 512
TaqI TCGA 2 cut(s) 667, 720
TasI AATT 5 cut(s) 42, 176, 196, 429, 646
TatI WGTACW 2 cut(s) 34, 620
TfiI GAWTC 1 cut(s) 739
Tru1I TTAA 2 cut(s) 513, 609
Tru9I TTAA 2 cut(s) 513, 609
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 2 cut(s) 184, 629
Tsp45I GTSAC 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 703
TspRI CASTG 1 cut(s) 592
VneI GTGCAC 1 cut(s) 464
XceI RCATGY 1 cut(s) 701
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.