Prupe.4G106400_v2.0.a1

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000525
Physical Location & Seq
Reverse (-)
5510248 .. 5517988
7741 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G106400.2

Sequence Viewer

Length: 2499 bp
ATGGAGGAAGATTGCTGCGCCACACAACTCATCTATGGGGATGGTCAGTTTAACTCCTCTGGGCTCGACAGGTTCGTCAAGGAAGTGAAGCTTGCTGAGTGTGGACTCTCCTATGCTGTGGTTGCCATCATGGGTCCTCAGAGCAGCGGGAAGAGCACTTTATTGAACCATCTGTTCCACACTAAGTTTAGGGAGATGGATGCATACAGTGGAAGGAGCCAAACAACTAAGGGTGTTTGGATAGCCAAGTGTGTTGGCATTGAGCCTTGCACGATTGCCATGGATTTGGAGGGTACTGATGGCAGGGAGAGAGGCGAGGATGATACTACATTCGAGAAGCAAAGTGCCCTATTTGCCCTGGCAGTTTCAGACATTGTGCTGATAAATATGTGGTGTCATGATATAGGTCGCGAGCAAGCTGCAAACAAACCTTTACTGAAAACAGTTTTTCAGGTCATGATGCGCTTATTCAGCCCCCGTAAAACGACATTGCTGTTTGTTATACGTGATAAAACAAAGACCCCGTTTGAATATCTGGAGCCGGTTTTGAGGGAAGATATACAGAAGATATGGGATGGAGTTCCGAAGCCCCAAGCCCATAAAAGTACCCCCTTCAGTGATTTCTTTAGTGTAGAAGTAGTTGCTTTGTCCAGTTATGAAGAGAAGGAGGAGAAGTTTAAGGAGGAGGTTGCTCAACTGAGGCAACGCTTTTTCCATTCTATTTCTCCAGGAGGGCTTGCTGGTGATAGACGGGGTGTTGTCCCTGCCACAGGATTTTCCTTTAGTGCACAACAGATTTGGAAAGTAATCAAAGAGAACAAGGACCTGGATCTTCCCGCTCACAAGGTTATGGTTGCTACTGTTCGGTGTGAAGAGATTGCCAACCAGAAATTTAATCAGTTAGTCTATGATGAGGATTGGTTGGCATTGGAAGAAGCTGTCCAAACGGGTCCCGTTCAAGGTTTTGGTAAAAGGCTTAGCTCCATCCTAGGCACCTATCTTTCTGAATATGACATGGAGGCCGTCTACTTTGACGAAGGAGTAAGGAACTCAAAACGACAATTGTTGGAGTCCAAAGCATTGGATTTTGTTTACCCTGCATACACGACCATGCTGGGACACCTACGCTCTAAAGCACTTGAAGATTTTAAAGTGAGGCTGGAGCAATCGTTAAACAAAGGAGGAGAATTTGCTTCATCTGTTCGTACCTCTACCCAGTCTTCCATGCTTGAGTTTGACAAAGGATGTGCAGATGCTGCCATACAACAAGCTGATTGGGATGCTTCGAGAGTCAGGGAAAAACTTAAACGTGATATAGATGCTCATGCATCATCCGTTCGTAGTGCAAAGTTGTCAGAACTGAATATCAATTATGAGAAACAACTTTCTGCATCTTTAAGTGGACCTGTAGAGGCACTACTTGAAACTGGTGGAAAAGACACTTGGACTTCAATAAGAAAACTTCTTAATCGTGAAACTGAAGTTGCAGTATCGAAGTTCTCAGCTGCAGTTGCTGGTTTTGAGTTGGACAAAGATACATCTACCAAAATGATGCAAAATTTAAGGGATTATGCAAGAAATGTGGTTGAGAAAAAAGCACGAGAAGAGGCTGCAAACATTATGATCCACATGAAGGATCGGTTCTCGACAGTCTTCAATTATGACAGTGATTCAATGCCTAGGGTTTGGACTGGGAAAGATGACATTAGAAGTATTACCAAGGATGCCCGCTCCGCGTCTCTAAAGCTTTTGTCAGTCATGGCTGCCATCCGCTTGGAGGAGAAGCCAGATAATATTGAAAAACTTCTCTTTTCTTCTTTGATGGATGGAACTGTTACCGTTTCATCTTCGCAAGATAGGAGGATAGCAGCTTCTACAGATCCTCTTGCCTCAAGCACTTGGGAAGAGGTTTCATCAAAGGATACTTTAATTACTCCAGTACAGTGCAAATCGTTGTGGAGACAGTTCAAGGCAGAGACTGAGTATAGTGTCACTCAAGCTATTGCAGCCCAGGAGGCTCACAAGCGGAGTAACAACTGGTTGCCTCCTCCATGGGCTATTATGGCGATGATTGTTCTTGGTTTTAATGAATTTATGCTCCTTTTAAAGAATCCTCTCTACCTCATGGTTCTATTTGTTGCATTTTTAATTTCAAAGGCCTTATGGGTACAGATGGACATTGCGGGAGAGTTCCAACATGGCACCCTGTCAGGAATACTATCTATTTCATCGAGGTTTCTTCCAACTGTCATGGATCTTCTAAGAAAACTTGCAGAAGAAGCTCAGGGTAATCCAGCACCTGAAGCACCCAGGCGGCCAGTATCTGTTGCTTCCCAGAGTCATAGAAATGAAACACCTCCGCCAAACACAATATCAAGTTCAATTCCTGAGTCATCTGTGTCATCTAATATCTCGTCATCGGATGGTGACGTCGAATACTCAAGCCCTCCCTTGAGACAAAGGCGACCTATGAATGTTCAAGAAGTTGAACCGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

833

Amino Acids

93.15

Weight (kDa)

5.78

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 1 cut(s) 2433
AccB1I GGYRCC 2 cut(s) 992, 2201
AccBSI CCGCTC 2 cut(s) 839, 1731
AccI GTMKAC 1 cut(s) 1026
AccII CGCG 2 cut(s) 411, 1736
AciI CCGC 9 cut(s) 147, 837, 1729, 1734, 1771, 2026, 2183, 2314, 2360
AclWI GGATC 5 cut(s) 837, 1618, 1644, 1874, 2262
AcoI YGGCCR 1 cut(s) 2315
AcsI RAATTY 4 cut(s) 890, 1187, 1558, 2090
AcuI CTGAAG 3 cut(s) 598, 1500, 2322
AcyI GRCGYC 1 cut(s) 2430
AfaI GTAC 5 cut(s) 295, 607, 1207, 1941, 2169
AfiI CCNNNNNNNGG 4 cut(s) 770, 959, 1633, 1777
AjnI CCWGG 5 cut(s) 357, 727, 825, 2010, 2309
AjuI GAANNNNNNNTTGG 2 cut(s) 1426, 1458
Alw21I GWGCWC 2 cut(s) 158, 790
Alw26I GTCTC 4 cut(s) 1743, 1954, 1970, 2449
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 5 cut(s) 837, 1618, 1644, 1874, 2262
AlwNI CAGNNNCTG 5 cut(s) 1256, 1514, 1979, 2300, 2324
AoxI GGCC 3 cut(s) 1020, 2157, 2315
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 9 cut(s) 15, 144, 419, 1256, 1505, 1610, 1763, 1868, 2006
ApoI RAATTY 4 cut(s) 890, 1187, 1558, 2090
Asp700I GAANNNNTTC 1 cut(s) 1803
AspA2I CCTAGG 2 cut(s) 988, 1679
AspLEI GCGC 2 cut(s) 20, 465
AspS9I GGNCC 4 cut(s) 134, 823, 950, 1403
AsuHPI GGTGA 2 cut(s) 755, 2438
AvaII GGWCC 4 cut(s) 134, 823, 950, 1403
AvrII CCTAGG 2 cut(s) 988, 1679
BaeGI GKGCMC 2 cut(s) 349, 790
BanI GGYRCC 2 cut(s) 992, 2201
BanII GRGCYC 1 cut(s) 66
BauI CACGAG 1 cut(s) 1599
BbsI GAAGAC 2 cut(s) 1212, 1645
Bbv12I GWGCWC 2 cut(s) 158, 790
BbvI GCAGC 9 cut(s) 2, 156, 406, 1243, 1492, 1597, 1750, 1880, 2018
BceAI ACGGC 1 cut(s) 1007
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 5 cut(s) 359, 729, 827, 2012, 2311
BciVI GTATCC 1 cut(s) 1915
BcoDI GTCTC 4 cut(s) 1743, 1954, 1970, 2449
BfaI CTAG 2 cut(s) 989, 1680
BfmI CTRYAG 3 cut(s) 1407, 1506, 1875
BfuI GTATCC 1 cut(s) 1915
BglI GCCNNNNNGGC 1 cut(s) 2015
BlnI CCTAGG 2 cut(s) 988, 1679
BlpI GCTNAGC 1 cut(s) 977
Bme1390I CCNGG 5 cut(s) 359, 729, 827, 2012, 2311
Bme18I GGWCC 4 cut(s) 134, 823, 950, 1403
BmgT120I GGNCC 4 cut(s) 134, 823, 950, 1403
BmiI GGNNCC 7 cut(s) 135, 218, 540, 951, 952, 994, 2203
BmrFI CCNGG 5 cut(s) 359, 729, 827, 2012, 2311
BmrI ACTGGG 2 cut(s) 1210, 1701
BmsI GCATC 9 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1400, 1542, 1714
BmuI ACTGGG 2 cut(s) 1210, 1701
BpiI GAAGAC 2 cut(s) 1212, 1645
BpmI CTGGAG 4 cut(s) 557, 711, 1181, 1920
Bpu10I CCTNAGC 1 cut(s) 2283
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 5 cut(s) 1250, 1876, 1980, 2425, 2473
BsaAI YACGTR 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 2430
BsaJI CCNNGG 8 cut(s) 279, 357, 988, 1679, 1719, 2010, 2051, 2309
BsaXI ACNNNNNCTCC 4 cut(s) 659, 689, 1154, 1184
Bsc4I CCNNNNNNNGG 4 cut(s) 770, 959, 1633, 1777
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 7 cut(s) 651, 1216, 1432, 1696, 1937, 2042, 2318
Bse3DI GCAATG 2 cut(s) 488, 2178
BseBI CCWGG 5 cut(s) 359, 729, 827, 2012, 2311
BseDI CCNNGG 8 cut(s) 279, 357, 988, 1679, 1719, 2010, 2051, 2309
BseLI CCNNNNNNNGG 4 cut(s) 770, 959, 1633, 1777
BseMI GCAATG 2 cut(s) 488, 2178
BseMII CTCAG 7 cut(s) 87, 152, 689, 1515, 1971, 2297, 2379
BseNI ACTGG 7 cut(s) 651, 1216, 1432, 1696, 1937, 2042, 2318
BseRI GAGGAG 6 cut(s) 46, 683, 698, 1197, 1793, 2037
BseSI GKGCMC 2 cut(s) 349, 790
BseXI GCAGC 9 cut(s) 2, 156, 406, 1243, 1492, 1597, 1750, 1880, 2018
BseYI CCCAGC 1 cut(s) 1114
BsgI GTGCAG 1 cut(s) 1269
Bsh1236I CGCG 2 cut(s) 411, 1736
BshFI GGCC 3 cut(s) 1022, 2159, 2317
BshNI GGYRCC 2 cut(s) 992, 2201
BsiHKAI GWGCWC 2 cut(s) 158, 790
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 3 cut(s) 746, 936, 1131
BslI CCNNNNNNNGG 4 cut(s) 770, 959, 1633, 1777
BsmAI GTCTC 4 cut(s) 1743, 1954, 1970, 2449
BsmBI CGTCTC 1 cut(s) 1743
BsmFI GGGAC 3 cut(s) 746, 936, 1131
BsnI GGCC 3 cut(s) 1022, 2159, 2317
Bsp1286I GDGCHC 4 cut(s) 66, 158, 349, 790
Bsp143I GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
Bsp1720I GCTNAGC 1 cut(s) 977
Bsp19I CCATGG 2 cut(s) 279, 2051
Bsp68I TCGCGA 1 cut(s) 411
BspACI CCGC 9 cut(s) 147, 837, 1729, 1734, 1771, 2026, 2183, 2314, 2360
BspANI GGCC 3 cut(s) 1022, 2159, 2317
BspCNI CTCAG 7 cut(s) 88, 151, 690, 1514, 1972, 2296, 2380
BspFNI CGCG 2 cut(s) 411, 1736
BspHI TCATGA 2 cut(s) 397, 456
BspLI GGNNCC 7 cut(s) 135, 218, 540, 951, 952, 994, 2203
BspMAI CTGCAG 1 cut(s) 1510
BspPI GGATC 5 cut(s) 837, 1618, 1644, 1874, 2262
BspQI GCTCTTC 1 cut(s) 146
BspT107I GGYRCC 2 cut(s) 992, 2201
BsrBI CCGCTC 2 cut(s) 839, 1731
BsrDI GCAATG 2 cut(s) 488, 2178
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 7 cut(s) 651, 1216, 1432, 1696, 1937, 2042, 2318
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 8 cut(s) 279, 357, 988, 1679, 1719, 2010, 2051, 2309
BssMI GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
BssNI GRCGYC 1 cut(s) 2430
BssSI CACGAG 1 cut(s) 1599
BssT1I CCWWGG 5 cut(s) 279, 988, 1679, 1719, 2051
Bst2BI CACGAG 1 cut(s) 1599
Bst2UI CCWGG 5 cut(s) 359, 729, 827, 2012, 2311
Bst6I CTCTTC 5 cut(s) 146, 654, 867, 1599, 1899
BstACI GRCGYC 1 cut(s) 2430
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 5 cut(s) 93, 413, 417, 738, 1729
BstDSI CCRYGG 2 cut(s) 279, 2051
BstENI CCTNNNNNAGG 1 cut(s) 768
BstFNI CGCG 2 cut(s) 411, 1736
BstHHI GCGC 2 cut(s) 20, 465
BstKTI GATC 5 cut(s) 832, 1626, 1639, 1882, 2257
BstMAI GTCTC 4 cut(s) 1743, 1954, 1970, 2449
BstMBI GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
BstNI CCWGG 5 cut(s) 359, 729, 827, 2012, 2311
BstSCI CCNGG 5 cut(s) 357, 727, 825, 2010, 2309
BstSFI CTRYAG 3 cut(s) 1407, 1506, 1875
BstSLI GKGCMC 2 cut(s) 349, 790
BstUI CGCG 2 cut(s) 411, 1736
BstV1I GCAGC 9 cut(s) 2, 156, 406, 1243, 1492, 1597, 1750, 1880, 2018
BstV2I GAAGAC 2 cut(s) 1212, 1645
BstX2I RGATCY 3 cut(s) 829, 1879, 2254
BstXI CCANNNNNNTGG 3 cut(s) 286, 1081, 1774
BstYI RGATCY 3 cut(s) 829, 1879, 2254
BsuI GTATCC 1 cut(s) 1915
BsuRI GGCC 3 cut(s) 1022, 2159, 2317
BtgI CCRYGG 2 cut(s) 279, 2051
BtgZI GCGATG 1 cut(s) 2081
BtsIMutI CAGTG 4 cut(s) 214, 622, 1672, 1949
BtuMI TCGCGA 1 cut(s) 411
Cac8I GCNNGC 5 cut(s) 93, 413, 417, 738, 1729
CaiI CAGNNNCTG 5 cut(s) 1256, 1514, 1979, 2300, 2324
CciI TCATGA 2 cut(s) 397, 456
CfoI GCGC 2 cut(s) 20, 465
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 4 cut(s) 134, 823, 950, 1403
CseI GACGC 1 cut(s) 1725
Csp6I GTAC 5 cut(s) 294, 606, 1206, 1940, 2168
CspCI CAANNNNNGTGG 2 cut(s) 1563, 1598
CviQI GTAC 5 cut(s) 294, 606, 1206, 1940, 2168
DpnI GATC 5 cut(s) 831, 1625, 1638, 1881, 2256
DpnII GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
DraI TTTAAA 2 cut(s) 1150, 2106
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 2315
Eam1104I CTCTTC 5 cut(s) 146, 654, 867, 1599, 1899
EarI CTCTTC 5 cut(s) 146, 654, 867, 1599, 1899
EciI GGCGGA 1 cut(s) 2349
Eco130I CCWWGG 5 cut(s) 279, 988, 1679, 1719, 2051
Eco147I AGGCCT 1 cut(s) 2159
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 4 cut(s) 134, 823, 950, 1403
Eco57I CTGAAG 3 cut(s) 598, 1500, 2322
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoO109I RGGNCCY 3 cut(s) 134, 823, 950
EcoRII CCWGG 5 cut(s) 357, 727, 825, 2010, 2309
EcoT14I CCWWGG 5 cut(s) 279, 988, 1679, 1719, 2051
EcoT22I ATGCAT 2 cut(s) 205, 1330
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 5 cut(s) 279, 988, 1679, 1719, 2051
Esp3I CGTCTC 1 cut(s) 1743
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 3 cut(s) 746, 936, 1131
FauI CCCGC 4 cut(s) 140, 844, 1736, 2176
FblI GTMKAC 1 cut(s) 1026
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 2 cut(s) 989, 1680
GlaI GCGC 2 cut(s) 19, 464
GsaI CCCAGC 1 cut(s) 1118
GsuI CTGGAG 4 cut(s) 557, 711, 1181, 1920
HaeIII GGCC 3 cut(s) 1022, 2159, 2317
HapII CCGG 1 cut(s) 542
HgaI GACGC 1 cut(s) 1725
HhaI GCGC 2 cut(s) 20, 465
Hin1I GRCGYC 1 cut(s) 2430
Hin6I GCGC 2 cut(s) 18, 463
HinP1I GCGC 2 cut(s) 18, 463
HindIII AAGCTT 2 cut(s) 89, 1745
HinfI GANTC 7 cut(s) 105, 1070, 1290, 1670, 2110, 2338, 2390
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 755, 2438
Hpy166II GTNNAC 5 cut(s) 104, 788, 1027, 1093, 1403
Hpy188I TCNGA 6 cut(s) 141, 370, 585, 1006, 1357, 2422
Hpy8I GTNNAC 5 cut(s) 104, 788, 1027, 1093, 1403
Hpy99I CGWCG 1 cut(s) 2435
HpyAV CCTTC 5 cut(s) 207, 622, 658, 1031, 1627
HpyCH4IV ACGT 3 cut(s) 505, 1309, 2430
HpySE526I ACGT 3 cut(s) 505, 1309, 2430
Hsp92I GRCGYC 1 cut(s) 2430
HspAI GCGC 2 cut(s) 18, 463
KflI GGGWCCC 1 cut(s) 950
Kzo9I GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 6 cut(s) 216, 538, 986, 1162, 1736, 2103
Lsp1109I GCAGC 9 cut(s) 2, 156, 406, 1243, 1492, 1597, 1750, 1880, 2018
LweI GCATC 9 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1400, 1542, 1714
MaeI CTAG 2 cut(s) 989, 1680
MaeII ACGT 3 cut(s) 505, 1309, 2430
MaeIII GTNAC 4 cut(s) 1834, 1990, 2030, 2426
MalI GATC 5 cut(s) 831, 1625, 1638, 1881, 2256
MbiI CCGCTC 2 cut(s) 839, 1731
MboI GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
MfeI CAATTG 1 cut(s) 1061
MflI RGATCY 3 cut(s) 829, 1879, 2254
MhlI GDGCHC 4 cut(s) 66, 158, 349, 790
MlyI GAGTC 5 cut(s) 99, 1079, 1299, 2347, 2399
MmeI TCCRAC 4 cut(s) 1047, 1506, 2218, 2267
Mph1103I ATGCAT 2 cut(s) 205, 1330
MroXI GAANNNNTTC 1 cut(s) 1803
MslI CAYNNNNRTG 2 cut(s) 1109, 2346
MspA1I CMGCKG 2 cut(s) 147, 1505
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 5 cut(s) 359, 729, 827, 2012, 2311
MunI CAATTG 1 cut(s) 1061
MvaI CCWGG 5 cut(s) 359, 729, 827, 2012, 2311
MvnI CGCG 2 cut(s) 411, 1736
NcoI CCATGG 2 cut(s) 279, 2051
NdeII GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
NlaIV GGNNCC 7 cut(s) 135, 218, 540, 951, 952, 994, 2203
NmuCI GTSAC 2 cut(s) 1990, 2426
NruI TCGCGA 1 cut(s) 411
NsiI ATGCAT 2 cut(s) 205, 1330
PagI TCATGA 2 cut(s) 397, 456
PceI AGGCCT 1 cut(s) 2159
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 1803
PfeI GAWTC 2 cut(s) 1670, 2110
PfoI TCCNGGA 1 cut(s) 727
PleI GAGTC 5 cut(s) 99, 1078, 1298, 2346, 2398
PpsI GAGTC 5 cut(s) 99, 1078, 1298, 2346, 2398
Ppu21I YACGTR 1 cut(s) 506
PpuMI RGGWCCY 3 cut(s) 134, 823, 950
Psp5II RGGWCCY 3 cut(s) 134, 823, 950
Psp6I CCWGG 5 cut(s) 357, 727, 825, 2010, 2309
PspFI CCCAGC 1 cut(s) 1114
PspGI CCWGG 5 cut(s) 357, 727, 825, 2010, 2309
PspN4I GGNNCC 7 cut(s) 135, 218, 540, 951, 952, 994, 2203
PspPI GGNCC 4 cut(s) 134, 823, 950, 1403
PspPPI RGGWCCY 3 cut(s) 134, 823, 950
PstI CTGCAG 1 cut(s) 1510
PstNI CAGNNNCTG 5 cut(s) 1256, 1514, 1979, 2300, 2324
PsuI RGATCY 3 cut(s) 829, 1879, 2254
PvuII CAGCTG 1 cut(s) 1505
RruI TCGCGA 1 cut(s) 411
RsaI GTAC 5 cut(s) 295, 607, 1207, 1941, 2169
RsaNI GTAC 5 cut(s) 294, 606, 1206, 1940, 2168
RseI CAYNNNNRTG 2 cut(s) 1109, 2346
SapI GCTCTTC 1 cut(s) 146
Sau3AI GATC 5 cut(s) 829, 1623, 1636, 1879, 2254
Sau96I GGNCC 4 cut(s) 134, 823, 950, 1403
SchI GAGTC 5 cut(s) 99, 1079, 1299, 2347, 2399
ScrFI CCNGG 5 cut(s) 359, 729, 827, 2012, 2311
SduI GDGCHC 4 cut(s) 66, 158, 349, 790
SfaNI GCATC 9 cut(s) 190, 450, 1243, 1270, 1309, 1337, 1400, 1542, 1714
SfcI CTRYAG 3 cut(s) 1407, 1506, 1875
SinI GGWCC 4 cut(s) 134, 823, 950, 1403
SmiMI CAYNNNNRTG 2 cut(s) 1109, 2346
SmlI CTYRAG 5 cut(s) 1229, 1891, 1995, 2440, 2452
SmoI CTYRAG 5 cut(s) 1229, 1891, 1995, 2440, 2452
SseBI AGGCCT 1 cut(s) 2159
SsiI CCGC 9 cut(s) 147, 837, 1729, 1734, 1771, 2026, 2183, 2314, 2360
SspI AATATT 1 cut(s) 1795
SspMI CTAG 2 cut(s) 989, 1680
StuI AGGCCT 1 cut(s) 2159
StyD4I CCNGG 5 cut(s) 357, 727, 825, 2010, 2309
StyI CCWWGG 5 cut(s) 279, 988, 1679, 1719, 2051
TaiI ACGT 3 cut(s) 508, 1312, 2433
TaqI TCGA 7 cut(s) 66, 333, 1286, 1493, 1646, 2231, 2433
TatI WGTACW 1 cut(s) 1939
TauI GCSGC 1 cut(s) 2317
TfiI GAWTC 2 cut(s) 1670, 2110
TscAI CASTG 4 cut(s) 214, 622, 1672, 1949
TseFI GTSAC 2 cut(s) 1990, 2426
TseI GCWGC 9 cut(s) 15, 144, 419, 1256, 1505, 1610, 1763, 1868, 2006
Tsp45I GTSAC 2 cut(s) 1990, 2426
TspDTI ATGAA 9 cut(s) 672, 1185, 1646, 1833, 1902, 2103, 2217, 2364, 2486
TspGWI ACGGA 1 cut(s) 1324
TspRI CASTG 4 cut(s) 214, 622, 1672, 1949
VneI GTGCAC 1 cut(s) 786
VpaK11BI GGWCC 4 cut(s) 134, 823, 950, 1403
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 4 cut(s) 890, 1187, 1558, 2090
XmaJI CCTAGG 2 cut(s) 988, 1679
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 1803
XspI CTAG 2 cut(s) 989, 1680
ZraI GACGTC 1 cut(s) 2431
Zsp2I ATGCAT 2 cut(s) 205, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.