Rh5BG139700

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000525
Physical Location & Seq
Reverse (-)
14359510 .. 14393670
34161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG139700.1

Sequence Viewer

Length: 2403 bp
ATGGATGAGTTGTCAGAGAAGCCAAGCCAGACAGAATGCAAGTATTACTTAAGGCCAGGGGGATGTAAGTATGGGAAAGCTTGCAGATACAACCATGGAAAAGGGAAACCTTCGGTTGCTCCAGTTGTGGAACTTAACTTTCTGGGACTGCCAATTCGACGGGGGGAGAGAGAGTGTCCCTACTATATGCGAAATGGCTCCTGCAAGTATGGATCAAACTGCAGGTTTAATCACCCTGATCCTACTGCTGCAGGAGGATCTGACCCTCCATCTGGATTTGATAATGGTGGACCTGCATCATTACAAGGTGGATCGCAATCATCATCTTGGTCTGCACCAAGATCATTGAATGAGACTCCGCTTTATATGCCAATGATGATGCCACCATCTCAAGGGGTTCCTTCTCAAAATACAGAATGGAATGGCTATCAGGCACCAGTTTATCTAGAAAGAAGCATGCCTGCACGTCCACCATATGTTATCAACAACTCAGGGACCAAAACCAATGTTTATAAACAATATCCACCGTCAAATCAAGTTGATGAATTTCCAGAACGACCTGGACAACGTTTGTGCAGTTTCTTTTTAAGAACAGGGGACTGTAAGTTTAAATCTAATTGCAAATATCATCATCCAAAAAGTCAGACTGCAGTATCCCCCTCATTTGCACCGAGTGACAAGGGCCTGCCTTTGAGACCACTTAATTTTCTCTCTAAGCTTGGAAGGGCCGGTAGGTACGTACGAATGGAGGAACCAATTGGAGAATACTGCTGCCCCGTGCAAATCATCGACTCTGACGGTGATTTCAAGAATGCTGGGCTTGACCGTTTCATTGAGCAAGCGAAGCTTGGTGAATGCGGGGCCTCATATGTTGTCACCTCCATCATAGGCGCTCAGAGTAGCGGGAAGAGCACCTTAATGAATCATCTTTTCGGCACCGGATTCACTGAGATGGACATAAACAAGAGAAGGTGCCAAACAACAAAGGGGATTTGGATAGCTAGATGTGCTGGTATTGAACCTTGTACATTGGCCATGGATGTGGAGGGCACGGATGGCAGGGAAAGAGGGGAGGATGATACATTTGAGAGACAAAGTGCGCTATTTGCACTCGCTATTTCGGACATTGTACTAATAAATATGTGGTGCCATGATGTTGGTCGAGAGCATGCTGCGAACAAACCTTTACTAAGAACAATTTTTGAGGTTATGATGCATTCATTTGACCCTGCCCGTAAGAAAACATTAATATTTATTTTACGGGATAAAACAAAGTCCTCGCTTGAACTTCTCCAACTTGACTTAAAAGATGATATACGAAGGATATGGAATGCAGTTCCCAAGCCCAGAGACCATGAAAGTACCCTGCTCGAAGAAATTTTCTCTGTGGAAGTAGTTGCTTTGTCCAGTTATGAAGATAAGGAAAAGGAGTTTCAGGAGGAGGTTGCTCAATTGAAGCAACGATTTATCAATTCTACCTCCCCCAGAGGGCTTGCGGGTGGTGACCGAACGGGTGTTCTGCCTGCCTCAGAATTTTCTCATTATGCACAGGAGATTTGGACACAAATAAAAAAGAACAGGGACCTGGATATTGCTTCCCACAAGAAATCGATTGCCGCTTTTCGGTGTGAAGAGATTGCCAACAAGAAATTCTTGCAGCTCAGCTTTGATGAGAATTGGTTGGCACTAGAAACGACTGTTCAAACTGATCTTGTAAGGGATTTTGGTGGAAAGCTCAGCTCAATTCTAAACATTTACATTTCTGGATATGACAAGGAGACAGAGTACTTTGATGAACTTATTAGGAGCTCGAAACGGCATCAACTGTTGGTGACAAAGGCATTCGATTTGTTTCGACCTGCATACGCCATCATGCTGAGACACCTCCGTTCCGAAGCACTTGAAAATTTTAAAATAAAACTGGAGCGGTCATTAGTCCAAAGAGAAGGGTTTGCTTCATCCGTTATTGCCCACACTGAATCTTGCATGCGTGAGTTTGACCAAGCATGTGCAGATGCTTCGATAGATGCTGCCATAAATTCTAATGGGAATTCATATGGCGTTCATGAGGAAAAAGCCAAAAGGAAACTTAAGCGCGATGTAGAAGCATATGGATCATCTCAACTTGACGAACAATTGTCAAGATTGAAACTGGACTATGAGGAGACTCAGAGACGAAATAACGCGCCACATGACTCAGAATTTAAACCATATATGGGGATAATTGTGGTTATCATAGGGTTGTTGTCAGCCTACGGAATCCCTAACGCCGCATCTTTTGTACCTGAAATTCTAGGACTGCTGCTGCCACGCTTAAGAAGTCAAGCTCTTACTTTTTCAGCCATATTTCTAGCTGTGCAGAAATTCGCAGAGGCCTACAGAAATTGGCGCTCGCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

800

Amino Acids

90.12

Weight (kDa)

8.24

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 55 - 79 8.4e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH_4 PF18044 59 - 79 5.6e-07 CCCH-type zinc finger
zf-CCCH PF00642 187 - 213 1.3e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
RHD3_GTPase PF05879 296 - 531 3.5e-96 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 574 - 721 1.8e-19 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
Acc36I ACCTGC 3 cut(s) 213, 301, 1867
AccB1I GGYRCC 4 cut(s) 433, 935, 972, 1146
AccBSI CCGCTC 1 cut(s) 1927
AccII CGCG 2 cut(s) 2097, 2186
AciI CCGC 7 cut(s) 359, 858, 903, 1496, 1617, 1927, 2271
AclI AACGTT 1 cut(s) 568
AclWI GGATC 5 cut(s) 220, 233, 265, 319, 2122
AcoI YGGCCR 1 cut(s) 1032
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 7 cut(s) 737, 741, 1027, 1131, 1363, 1787, 2283
AfiI CCNNNNNNNGG 5 cut(s) 272, 392, 1488, 1623, 2216
AflII CTTAAG 3 cut(s) 49, 2090, 2314
AgsI TTSAA 8 cut(s) 349, 808, 1019, 1286, 1456, 1703, 1904, 2149
AjiI CACGTC 1 cut(s) 467
AjnI CCWGG 3 cut(s) 55, 559, 1584
AjuI GAANNNNNNNTTGG 2 cut(s) 1634, 1666
Alw21I GWGCWC 2 cut(s) 914, 1811
Alw26I GTCTC 8 cut(s) 347, 688, 1084, 1344, 1772, 1873, 2159, 2167
AlwI GGATC 5 cut(s) 220, 233, 265, 319, 2122
AoxI GGCC 6 cut(s) 53, 682, 726, 861, 1032, 2373
ApeKI GCWGC 7 cut(s) 248, 771, 1172, 1657, 2030, 2302, 2305
ArsI GACNNNNNNTTYG 2 cut(s) 138, 170
AseI ATTAAT 1 cut(s) 1247
AspLEI GCGC 5 cut(s) 893, 1102, 2097, 2188, 2391
AspS9I GGNCC 6 cut(s) 290, 495, 682, 726, 861, 1582
AsuHPI GGTGA 6 cut(s) 224, 812, 863, 868, 1514, 1843
AvaII GGWCC 3 cut(s) 290, 495, 1582
BaeGI GKGCMC 1 cut(s) 1052
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BalI TGGCCA 1 cut(s) 1034
BanI GGYRCC 4 cut(s) 433, 935, 972, 1146
BanII GRGCYC 1 cut(s) 1811
Bbv12I GWGCWC 2 cut(s) 914, 1811
BbvI GCAGC 7 cut(s) 235, 758, 1159, 1669, 2017, 2289, 2292
BccI CCATC 6 cut(s) 277, 394, 890, 946, 1049, 1877
BceAI ACGGC 1 cut(s) 1832
BcgI CGANNNNNNTGC 2 cut(s) 2001, 2035
BciT130I CCWGG 3 cut(s) 57, 561, 1586
BciVI GTATCC 1 cut(s) 664
BcoDI GTCTC 8 cut(s) 347, 688, 1084, 1344, 1772, 1873, 2159, 2167
BfaI CTAG 5 cut(s) 446, 1002, 1688, 2294, 2351
BfmI CTRYAG 4 cut(s) 220, 249, 648, 2377
BfoI RGCGCY 2 cut(s) 894, 2392
BfrI CTTAAG 3 cut(s) 49, 2090, 2314
BfuAI ACCTGC 3 cut(s) 213, 301, 1867
BfuI GTATCC 1 cut(s) 664
BisI GCNGC 9 cut(s) 249, 772, 1173, 1617, 1658, 2031, 2271, 2303, 2306
BlpI GCTNAGC 2 cut(s) 1661, 1736
BlsI GCNGC 9 cut(s) 250, 773, 1174, 1618, 1659, 2032, 2272, 2304, 2307
BmcAI AGTACT 1 cut(s) 1787
Bme1390I CCNGG 3 cut(s) 57, 561, 1586
Bme18I GGWCC 3 cut(s) 290, 495, 1582
BmgBI CACGTC 1 cut(s) 467
BmgT120I GGNCC 6 cut(s) 290, 495, 682, 726, 861, 1582
BmrFI CCNGG 3 cut(s) 57, 561, 1586
BmsI GCATC 7 cut(s) 305, 369, 1203, 1828, 2005, 2017, 2282
BpmI CTGGAG 2 cut(s) 105, 1943
Bpu1102I GCTNAGC 2 cut(s) 1661, 1736
BpuEI CTTGAG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 1610
BsaAI YACGTR 1 cut(s) 739
BsaBI GATNNNNATC 1 cut(s) 316
BsaI GGTCTC 2 cut(s) 688, 1344
BsaJI CCNNGG 3 cut(s) 56, 94, 1035
BsaWI WCCGGW 1 cut(s) 938
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 1769, 1799
Bsc4I CCNNNNNNNGG 5 cut(s) 272, 392, 1488, 1623, 2216
Bse118I RCCGGY 1 cut(s) 728
Bse1I ACTGG 5 cut(s) 122, 437, 1407, 1926, 2157
Bse8I GATNNNNATC 1 cut(s) 316
BseBI CCWGG 3 cut(s) 57, 561, 1586
BseCI ATCGAT 1 cut(s) 1610
BseDI CCNNGG 3 cut(s) 56, 94, 1035
BseGI GGATG 7 cut(s) 10, 68, 631, 1045, 1060, 1081, 1958
BseJI GATNNNNATC 1 cut(s) 316
BseLI CCNNNNNNNGG 5 cut(s) 272, 392, 1488, 1623, 2216
BseMII CTCAG 9 cut(s) 504, 908, 939, 1542, 1675, 1750, 1868, 2183, 2211
BseNI ACTGG 5 cut(s) 122, 437, 1407, 1926, 2157
BseRI GAGGAG 2 cut(s) 1454, 2177
BseSI GKGCMC 1 cut(s) 1052
BseXI GCAGC 7 cut(s) 235, 758, 1159, 1669, 2017, 2289, 2292
BseYI CCCAGC 1 cut(s) 815
BsgI GTGCAG 5 cut(s) 318, 447, 595, 2031, 2378
Bsh1236I CGCG 2 cut(s) 2097, 2186
BshFI GGCC 6 cut(s) 55, 684, 728, 863, 1034, 2375
BshNI GGYRCC 4 cut(s) 433, 935, 972, 1146
BshVI ATCGAT 1 cut(s) 1610
BsiHKAI GWGCWC 2 cut(s) 914, 1811
BsiSI CCGG 2 cut(s) 729, 939
BsiWI CGTACG 1 cut(s) 739
BslFI GGGAC 5 cut(s) 159, 162, 508, 611, 1595
BslI CCNNNNNNNGG 5 cut(s) 272, 392, 1488, 1623, 2216
BsmAI GTCTC 8 cut(s) 347, 688, 1084, 1344, 1772, 1873, 2159, 2167
BsmBI CGTCTC 1 cut(s) 2167
BsmFI GGGAC 5 cut(s) 159, 162, 508, 611, 1595
BsmI GAATGC 6 cut(s) 41, 817, 860, 1216, 1336, 1841
BsnI GGCC 6 cut(s) 55, 684, 728, 863, 1034, 2375
Bso31I GGTCTC 2 cut(s) 688, 1344
Bsp1286I GDGCHC 3 cut(s) 914, 1052, 1811
Bsp1407I TGTACA 1 cut(s) 1025
Bsp143I GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
Bsp1720I GCTNAGC 2 cut(s) 1661, 1736
Bsp19I CCATGG 2 cut(s) 94, 1035
BspACI CCGC 7 cut(s) 359, 858, 903, 1496, 1617, 1927, 2271
BspANI GGCC 6 cut(s) 55, 684, 728, 863, 1034, 2375
BspCNI CTCAG 9 cut(s) 503, 907, 940, 1541, 1674, 1749, 1869, 2182, 2210
BspDI ATCGAT 1 cut(s) 1610
BspFNI CGCG 2 cut(s) 2097, 2186
BspHI TCATGA 1 cut(s) 2065
BspMAI CTGCAG 3 cut(s) 224, 253, 652
BspMI ACCTGC 3 cut(s) 213, 301, 1867
BspPI GGATC 5 cut(s) 220, 233, 265, 319, 2122
BspQI GCTCTTC 1 cut(s) 902
BspT107I GGYRCC 4 cut(s) 433, 935, 972, 1146
BspTI CTTAAG 3 cut(s) 49, 2090, 2314
BspTNI GGTCTC 2 cut(s) 688, 1344
BsrBI CCGCTC 1 cut(s) 1927
BsrFI RCCGGY 1 cut(s) 728
BsrGI TGTACA 1 cut(s) 1025
BsrI ACTGG 5 cut(s) 122, 437, 1407, 1926, 2157
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 3 cut(s) 56, 94, 1035
BssMI GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
BssT1I CCWWGG 2 cut(s) 94, 1035
Bst2UI CCWGG 3 cut(s) 57, 561, 1586
Bst4CI ACNGT 6 cut(s) 528, 602, 800, 827, 1699, 1827
Bst6I CTCTTC 2 cut(s) 902, 1626
BstAFI CTTAAG 3 cut(s) 49, 2090, 2314
BstAUI TGTACA 1 cut(s) 1025
BstBAI YACGTR 1 cut(s) 739
BstDSI CCRYGG 2 cut(s) 94, 1035
BstEII GGTNACC 1 cut(s) 1502
BstF5I GGATG 7 cut(s) 10, 68, 631, 1045, 1060, 1081, 1958
BstFNI CGCG 2 cut(s) 2097, 2186
BstH2I RGCGCY 2 cut(s) 894, 2392
BstHHI GCGC 5 cut(s) 893, 1102, 2097, 2188, 2391
BstKTI GATC 7 cut(s) 215, 241, 260, 314, 344, 1711, 2117
BstMAI GTCTC 8 cut(s) 347, 688, 1084, 1344, 1772, 1873, 2159, 2167
BstMBI GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
BstMWI GCNNNNNNNGC 6 cut(s) 367, 844, 909, 1007, 1056, 1106
BstNI CCWGG 3 cut(s) 57, 561, 1586
BstNSI RCATGY 4 cut(s) 460, 1172, 1990, 2010
BstPI GGTNACC 1 cut(s) 1502
BstSCI CCNGG 3 cut(s) 55, 559, 1584
BstSFI CTRYAG 4 cut(s) 220, 249, 648, 2377
BstSLI GKGCMC 1 cut(s) 1052
BstSNI TACGTA 1 cut(s) 739
BstUI CGCG 2 cut(s) 2097, 2186
BstV1I GCAGC 7 cut(s) 235, 758, 1159, 1669, 2017, 2289, 2292
BstX2I RGATCY 1 cut(s) 257
BstXI CCANNNNNNTGG 2 cut(s) 1042, 1157
BstYI RGATCY 1 cut(s) 257
Bsu15I ATCGAT 1 cut(s) 1610
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 6 cut(s) 55, 684, 728, 863, 1034, 2375
BsuTUI ATCGAT 1 cut(s) 1610
BtgI CCRYGG 2 cut(s) 94, 1035
BtgZI GCGATG 1 cut(s) 2112
BtrI CACGTC 1 cut(s) 467
BtsCI GGATG 7 cut(s) 10, 68, 631, 1045, 1060, 1081, 1958
BtsIMutI CAGTG 2 cut(s) 945, 1974
BveI ACCTGC 3 cut(s) 213, 301, 1867
CciI TCATGA 1 cut(s) 2065
CfoI GCGC 5 cut(s) 893, 1102, 2097, 2188, 2391
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 6 cut(s) 290, 495, 682, 726, 861, 1582
ClaI ATCGAT 1 cut(s) 1610
Csp6I GTAC 7 cut(s) 736, 740, 1026, 1130, 1362, 1786, 2282
CviQI GTAC 7 cut(s) 736, 740, 1026, 1130, 1362, 1786, 2282
DpnI GATC 7 cut(s) 214, 240, 259, 313, 343, 1710, 2116
DpnII GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
DraI TTTAAA 3 cut(s) 610, 1912, 2206
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 2 cut(s) 902, 1626
EarI CTCTTC 2 cut(s) 902, 1626
Ecl136II GAGCTC 1 cut(s) 1809
Eco105I TACGTA 1 cut(s) 739
Eco130I CCWWGG 2 cut(s) 94, 1035
Eco147I AGGCCT 1 cut(s) 2375
Eco24I GRGCYC 1 cut(s) 1811
Eco31I GGTCTC 2 cut(s) 688, 1344
Eco47I GGWCC 3 cut(s) 290, 495, 1582
Eco53kI GAGCTC 1 cut(s) 1809
Eco91I GGTNACC 1 cut(s) 1502
EcoICRI GAGCTC 1 cut(s) 1809
EcoO109I RGGNCCY 3 cut(s) 682, 861, 1582
EcoO65I GGTNACC 1 cut(s) 1502
EcoRI GAATTC 1 cut(s) 2050
EcoRII CCWGG 3 cut(s) 55, 559, 1584
EcoT14I CCWWGG 2 cut(s) 94, 1035
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 1811
ErhI CCWWGG 2 cut(s) 94, 1035
Esp3I CGTCTC 1 cut(s) 2167
FalI AAGNNNNNCTT 8 cut(s) 32, 64, 831, 863, 899, 931, 1637, 1669
FaqI GGGAC 5 cut(s) 159, 162, 508, 611, 1595
FauI CCCGC 3 cut(s) 851, 896, 1489
FauNDI CATATG 4 cut(s) 475, 868, 2056, 2110
Fnu4HI GCNGC 9 cut(s) 249, 772, 1173, 1617, 1658, 2031, 2271, 2303, 2306
FokI GGATG 7 cut(s) 17, 75, 618, 1052, 1067, 1088, 1945
FriOI GRGCYC 1 cut(s) 1811
Fsp4HI GCNGC 9 cut(s) 249, 772, 1173, 1617, 1658, 2031, 2271, 2303, 2306
FspBI CTAG 5 cut(s) 446, 1002, 1688, 2294, 2351
GlaI GCGC 5 cut(s) 892, 1101, 2096, 2187, 2390
GluI GCNGC 9 cut(s) 249, 772, 1173, 1617, 1658, 2031, 2271, 2303, 2306
GsaI CCCAGC 1 cut(s) 819
GsuI CTGGAG 2 cut(s) 105, 1943
HaeII RGCGCY 2 cut(s) 894, 2392
HaeIII GGCC 6 cut(s) 55, 684, 728, 863, 1034, 2375
HapII CCGG 2 cut(s) 729, 939
HhaI GCGC 5 cut(s) 893, 1102, 2097, 2188, 2391
Hin6I GCGC 5 cut(s) 891, 1100, 2095, 2186, 2389
HinP1I GCGC 5 cut(s) 891, 1100, 2095, 2186, 2389
HindIII AAGCTT 3 cut(s) 78, 716, 845
HinfI GANTC 8 cut(s) 355, 791, 922, 942, 1979, 2167, 2195, 2259
HpaII CCGG 2 cut(s) 729, 939
HphI GGTGA 6 cut(s) 224, 812, 863, 868, 1514, 1843
Hpy166II GTNNAC 2 cut(s) 290, 470
Hpy188III TCNNGA 9 cut(s) 273, 446, 551, 808, 1163, 1436, 1764, 2066, 2142
Hpy8I GTNNAC 2 cut(s) 290, 470
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 6 cut(s) 120, 411, 717, 963, 1314, 1940
HpyCH4III ACNGT 6 cut(s) 528, 602, 800, 827, 1699, 1827
HpyCH4IV ACGT 3 cut(s) 466, 568, 738
HpyF10VI GCNNNNNNNGC 6 cut(s) 367, 844, 909, 1007, 1056, 1106
HpySE526I ACGT 3 cut(s) 466, 568, 738
HspAI GCGC 5 cut(s) 891, 1100, 2095, 2186, 2389
Kzo9I GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
LguI GCTCTTC 1 cut(s) 902
LmnI GCTCC 4 cut(s) 124, 203, 1806, 1924
Lsp1109I GCAGC 7 cut(s) 235, 758, 1159, 1669, 2017, 2289, 2292
LweI GCATC 7 cut(s) 305, 369, 1203, 1828, 2005, 2017, 2282
MaeI CTAG 5 cut(s) 446, 1002, 1688, 2294, 2351
MaeII ACGT 3 cut(s) 466, 568, 738
MaeIII GTNAC 4 cut(s) 674, 874, 1502, 1831
MalI GATC 7 cut(s) 214, 240, 259, 313, 343, 1710, 2116
MbiI CCGCTC 1 cut(s) 1927
MboI GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
MboII GAAGA 4 cut(s) 919, 1385, 1427, 1643
MfeI CAATTG 3 cut(s) 756, 1451, 2135
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 3 cut(s) 914, 1052, 1811
MlsI TGGCCA 1 cut(s) 1034
MluNI TGGCCA 1 cut(s) 1034
MlyI GAGTC 4 cut(s) 349, 785, 2161, 2189
MmeI TCCRAC 1 cut(s) 1318
Mox20I TGGCCA 1 cut(s) 1034
Mph1103I ATGCAT 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1034
MslI CAYNNNNRTG 3 cut(s) 917, 950, 1040
Msp20I TGGCCA 1 cut(s) 1034
MspCI CTTAAG 3 cut(s) 49, 2090, 2314
MspI CCGG 2 cut(s) 729, 939
MspR9I CCNGG 3 cut(s) 57, 561, 1586
MunI CAATTG 3 cut(s) 756, 1451, 2135
Mva1269I GAATGC 6 cut(s) 41, 817, 860, 1216, 1336, 1841
MvaI CCWGG 3 cut(s) 57, 561, 1586
MvnI CGCG 2 cut(s) 2097, 2186
MwoI GCNNNNNNNGC 6 cut(s) 367, 844, 909, 1007, 1056, 1106
NcoI CCATGG 2 cut(s) 94, 1035
NdeI CATATG 4 cut(s) 475, 868, 2056, 2110
NdeII GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
NmuCI GTSAC 4 cut(s) 674, 874, 1502, 1831
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 4 cut(s) 460, 1172, 1990, 2010
PaeI GCATGC 3 cut(s) 460, 1172, 1990
PagI TCATGA 1 cut(s) 2065
PceI AGGCCT 1 cut(s) 2375
PciSI GCTCTTC 1 cut(s) 902
PctI GAATGC 6 cut(s) 41, 817, 860, 1216, 1336, 1841
PfeI GAWTC 4 cut(s) 922, 942, 1979, 2259
Pfl23II CGTACG 1 cut(s) 739
PkrI GCNGC 9 cut(s) 250, 773, 1174, 1618, 1659, 2032, 2272, 2304, 2307
PleI GAGTC 4 cut(s) 349, 785, 2161, 2189
PpsI GAGTC 4 cut(s) 349, 785, 2161, 2189
Ppu21I YACGTR 1 cut(s) 739
PpuMI RGGWCCY 1 cut(s) 1582
PshBI ATTAAT 1 cut(s) 1247
PsiI TTATAA 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 1811
Psp1406I AACGTT 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 3 cut(s) 55, 559, 1584
PspEI GGTNACC 1 cut(s) 1502
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 3 cut(s) 55, 559, 1584
PspLI CGTACG 1 cut(s) 739
PspPI GGNCC 6 cut(s) 290, 495, 682, 726, 861, 1582
PspPPI RGGWCCY 1 cut(s) 1582
PstI CTGCAG 3 cut(s) 224, 253, 652
PsuI RGATCY 1 cut(s) 257
RsaI GTAC 7 cut(s) 737, 741, 1027, 1131, 1363, 1787, 2283
RsaNI GTAC 7 cut(s) 736, 740, 1026, 1130, 1362, 1786, 2282
RseI CAYNNNNRTG 3 cut(s) 917, 950, 1040
SacI GAGCTC 1 cut(s) 1811
SapI GCTCTTC 1 cut(s) 902
SatI GCNGC 9 cut(s) 249, 772, 1173, 1617, 1658, 2031, 2271, 2303, 2306
Sau3AI GATC 7 cut(s) 212, 238, 257, 311, 341, 1708, 2114
Sau96I GGNCC 6 cut(s) 290, 495, 682, 726, 861, 1582
ScaI AGTACT 1 cut(s) 1787
SchI GAGTC 4 cut(s) 349, 785, 2161, 2189
ScrFI CCNGG 3 cut(s) 57, 561, 1586
SduI GDGCHC 3 cut(s) 914, 1052, 1811
SfaNI GCATC 7 cut(s) 305, 369, 1203, 1828, 2005, 2017, 2282
SfcI CTRYAG 4 cut(s) 220, 249, 648, 2377
SinI GGWCC 3 cut(s) 290, 495, 1582
SmiMI CAYNNNNRTG 3 cut(s) 917, 950, 1040
SmlI CTYRAG 4 cut(s) 49, 390, 2090, 2314
SmoI CTYRAG 4 cut(s) 49, 390, 2090, 2314
SnaBI TACGTA 1 cut(s) 739
SphI GCATGC 3 cut(s) 460, 1172, 1990
SseBI AGGCCT 1 cut(s) 2375
SsiI CCGC 7 cut(s) 359, 858, 903, 1496, 1617, 1927, 2271
SspI AATATT 1 cut(s) 1251
SspMI CTAG 5 cut(s) 446, 1002, 1688, 2294, 2351
SstI GAGCTC 1 cut(s) 1811
StuI AGGCCT 1 cut(s) 2375
StyD4I CCNGG 3 cut(s) 55, 559, 1584
StyI CCWWGG 2 cut(s) 94, 1035
TaaI ACNGT 6 cut(s) 528, 602, 800, 827, 1699, 1827
TaiI ACGT 3 cut(s) 469, 571, 741
TaqI TCGA 9 cut(s) 157, 789, 1162, 1371, 1610, 1811, 1845, 1855, 2021
TaqII GACCGA 1 cut(s) 1521
TatI WGTACW 3 cut(s) 1025, 1129, 1785
TauI GCSGC 2 cut(s) 1619, 2273
TfiI GAWTC 4 cut(s) 922, 942, 1979, 2259
TscAI CASTG 2 cut(s) 952, 1981
TseFI GTSAC 4 cut(s) 674, 874, 1502, 1831
TseI GCWGC 7 cut(s) 248, 771, 1172, 1657, 2030, 2302, 2305
Tsp45I GTSAC 4 cut(s) 674, 874, 1502, 1831
TspGWI ACGGA 4 cut(s) 1067, 1877, 1951, 2271
TspRI CASTG 2 cut(s) 952, 1981
Vha464I CTTAAG 3 cut(s) 49, 2090, 2314
VpaK11BI GGWCC 3 cut(s) 290, 495, 1582
VspI ATTAAT 1 cut(s) 1247
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 4 cut(s) 460, 1172, 1990, 2010
XspI CTAG 5 cut(s) 446, 1002, 1688, 2294, 2351
ZrmI AGTACT 1 cut(s) 1787
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.